A5072520782- Neurogenesis and neuroplasticity mechanisms
- Genetic and phenotypic traits in livestock
- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- RNA regulation and disease
- Congenital heart defects research
- Cellular transport and secretion
- Developmental Biology and Gene Regulation
- Zebrafish Biomedical Research Applications
- Microtubule and mitosis dynamics
- Genetic Mapping and Diversity in Plants and Animals
- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
- Autism Spectrum Disorder Research
- Genomics and Rare Diseases
- MicroRNA in disease regulation
- Vestibular and auditory disorders
- RNA modifications and cancer
- Muscle Physiology and Disorders
- Ubiquitin and proteasome pathways
- Neurobiology and Insect Physiology Research
- Mitochondrial Function and Pathology
- Pluripotent Stem Cells Research
- Histone Deacetylase Inhibitors Research
National Center of Neurology and Psychiatry
2016-2025
National Livestock Breeding Center
2016-2024
Niigata University
2022-2024
Cleveland Clinic
2024
Waseda University
2016-2018
Tottori University
2006
Cerebellar granule cell precursors (GCPs) and cells (GCs) represent good models to study neuronal development. Here, we report that the transcription factor myeloid ectopic viral integration site 1 homolog (Meis1) plays pivotal roles in regulation of mouse GC We found Meis1 is expressed lineage astrocytes cerebellum during Targeted disruption gene specifically resulted smaller cerebella with disorganized lobules. Knock-down/knock-out (KO) experiments for vitro assays showed binds an upstream...
Cerebrospinal fluid-contacting neurons (CSF-cNs) are enigmatic mechano- or chemosensory cells lying along the central canal of spinal cord. Recent studies in zebrafish larvae and lampreys have shown that CSF-cNs control postures movements via connections. However, structures, connectivity, functions mammals remain largely unknown. Here we developed a method to genetically target mouse highlighted structural connections functions. We first found intracerebroventricular injection...
The aim of the present study was to compare power single nucleotide polymorphism (SNP)-based genome-wide association (GWAS) and haplotype-based GWAS for quantitative trait loci (QTL) detection, detect novel candidate genes affecting economically important traits in a purebred Duroc population comprising seven-generation pedigree. First, we performed simulation analysis using real genotype data this (based on null hypothesis) two methods. We then both methods phenotype 52 traits, which...
Impairments in synapse development are thought to cause numerous psychiatric disorders. Autism susceptibility candidate 2 (AUTS2) gene has been associated with various disorders, such as autism and intellectual disabilities. Although roles for AUTS2 neuronal migration neuritogenesis have reported, its involvement regulation remains unclear. In this study, we found that excitatory synapses were specifically increased the Auts2-deficient primary cultured neurons well Auts2 mutant forebrains....
Duchenne muscular dystrophy (DMD) is an X-linked genetic disorder characterized by progressive weakness because of the loss dystrophin. Extracellular Ca2+ flows into cytoplasm through membrane tears in dystrophin-deficient myofibers, which leads to muscle contracture and necrosis. Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) takes up cytosolic sarcoplasmic reticulum, but its activity decreased dystrophic muscle. Here, we show that allosteric SERCA activator, CDN1163, ameliorates...
Abstract In the central nervous system, astrocytes enable appropriate synapse function through glutamate clearance from synaptic cleft; however, it remains unclear how astrocytic transporters at peri-synaptic contact. Here, we report that Down syndrome cell adhesion molecule (DSCAM) in Purkinje cells controls formation and developing cerebellum. Dscam -mutant mice show defects CF translocation as is observed loss of mutations transporter GLAST expressed Bergmann glia. These impaired...
Abstract AUTS2 syndrome is characterized by intellectual disability and microcephaly, often associated with autism spectrum disorder, but the underlying mechanisms, particularly concerning remain incompletely understood. Here, we analyze mice mutated for transcriptional regulator AUTS2, which recapitulate microcephaly. Their brains exhibit reduced division of intermediate progenitor cells (IPCs), leading to fewer neurons decreased thickness in upper-layer cortex. Increased expression target...
The mammalian brain undergoes sexual differentiation by gonadal hormones during the perinatal critical period. However, machinery at earlier stages has not been well studied. We found that Ptf1a is expressed in certain neuroepithelial cells and immature neurons around third ventricle give rise to various several hypothalamic nuclei. show conditional Ptf1a-deficient mice (Ptf1a cKO) exhibit abnormalities sex-biased behaviors reproductive organs both sexes. Gonadal hormone administration...
DSCAM regulates neuronal delamination from the ventricular surface by suppressing RapGEF2/Rap1 and N-cadherin.
Intensive chemotherapeutic regimens with craniospinal irradiation have greatly improved survival in medulloblastoma patients. However, markedly differs among molecular subgroups and their biomarkers are unknown. Through unbiased screening, we found Schlafen family member 11 (SLFN11), which is known to improve response DNA damaging agents various cancers, be one of the top prognostic markers medulloblastomas. Hence, explored expression functions SLFN11 medulloblastoma.SLFN11 for each subgroup...
Abstract The aim of the present study was to detect quantitative trait loci affecting fatty acid composition in back fat and intramuscular a Duroc pig population comprising seventh‐generation pedigrees using genome‐wide association studies ( GWAS ). In total, 305 animals were genotyped single nucleotide polymorphisms SNP s) array five selected SNPs from regions containing known candidate genes related synthesis or metabolism. 24 significant detected 12 traits, 76 suggestive 33 traits. Sus...
Cerebellar granule cells (GCs) are which comprise over 50% of the neurons in entire nervous system. GCs enable cerebellum to properly regulate motor coordination, learning, and consolidation, addition cognition, emotion language. During GC development, maternal progenitors (GCPs) divide produce not only postmitotic but also sister GCPs. However, molecular machinery for regulating proportional production distinct cell types from seemingly uniform GCPs is yet fully understood. Here we report...
Abstract Low‐grade neuroepithelial tumors are major causes of drug‐resistant focal epilepsy. Clinically, these defined as low‐grade epilepsy‐associated (LEATs). The BRAF V600E mutation is frequently observed in LEAT and linked to poor seizure outcomes. However, its molecular role epileptogenicity remains elusive. To understand the mechanism underlying with genetic (BRAF V600E‐LEAT), we conducted RNA sequencing (RNA‐seq) analysis using surgical specimens V600E‐LEAT obtained stored at a single...
A simulation analysis and real phenotype were performed to evaluate the impact of three different relationship matrices on heritability estimation prediction accuracy in a closed-line breeding Duroc pigs. The numerator matrix (NRM), single nucleotide polymorphism (SNP)-based genomic (GRM) (GS ), haplotype-based GRM (GH ) applied this study. We used PorcineSNP60 genotype array data (38 114 SNPs) 831 pigs with four selection traits. In both accuracy, depended number animals records. For...
Abstract Impairments in synapse development are thought to cause numerous psychiatric disorders. Autism susceptibility candidate 2 ( AUTS2 ) gene has been associated with various disorders, such as autism and intellectual disabilities. Although roles for neuronal migration neuritogenesis have reported, its involvement regulation remains unclear. In this study, we found that excitatory synapses were specifically increased the Auts2 -deficient primary cultured neurons well mutant forebrains....
Abstract AUTS2 mutations often exhibit neurodevelopmental disorders and microcephaly. However, how regulates neuron production affects brain size remains unclear. Here, we show that cooperates with the Polycomb complex PRC2 to regulate gene expression cortical neurogenesis. Auts2 mutant mice reduced division of intermediate progenitor cells (IPCs), leading decreased neurons thickness in upper-layer cortex. Expression Robo1 is increased mutants, which turn suppresses IPC division....
Brain arteriovenous malformations (bAVMs) are anomalies forming vascular tangles connecting the arteries and veins, which cause hemorrhagic stroke in young adults. Current surgical approaches highly invasive, alternative therapeutic methods warranted. Recent genetic studies identified KRAS mutations endothelial cells of bAVMs; however, underlying process leading to malformation postnatal stage remains unknown. Here we established a mouse model bAVM developing during early stage. Among 4...
Reproduction, Fertility and Development is an international journal publishing original research , review comment in the fields of reproduction developmental biology humans, domestic animals wildlife