A5043672301- Hormonal Regulation and Hypertension
- Sodium Intake and Health
- Blood Pressure and Hypertension Studies
- Ion Transport and Channel Regulation
- Renin-Angiotensin System Studies
- Cardiovascular Health and Disease Prevention
- Atherosclerosis and Cardiovascular Diseases
- Heart Rate Variability and Autonomic Control
- Adipokines, Inflammation, and Metabolic Diseases
- Electrolyte and hormonal disorders
- Cardiovascular Function and Risk Factors
- Cardiac Imaging and Diagnostics
- Hemodynamic Monitoring and Therapy
- Cancer, Stress, Anesthesia, and Immune Response
- Thermoregulation and physiological responses
- Advanced MRI Techniques and Applications
- Nutritional Studies and Diet
- Cancer-related cognitive impairment studies
- Cardiovascular Disease and Adiposity
- Cancer Immunotherapy and Biomarkers
- Adipose Tissue and Metabolism
- Receptor Mechanisms and Signaling
- Dialysis and Renal Disease Management
- Chronic Kidney Disease and Diabetes
- Aortic Disease and Treatment Approaches
Yokohama City University
2016-2025
Duke-NUS Medical School
2020-2024
National University of Singapore
2023
Yokohama City University Hospital
2016-2018
Kanagawa Cardiovascular and Respiratory Center
2017-2018
Red Cross Hospital
2017
International University Of Health And Welfare Atami Hospital
2009
International University of Health and Welfare
2009
Sodium-glucose cotransporter 2 inhibitors are believed to improve cardiac outcomes due their osmotic diuretic potential. The goal of this study was test the hypothesis that vasopressin-driven urine concentration overrides effect glucosuria induced by dapagliflozin treatment. DAPA-Shuttle1 (Hepato-renal Regulation Water Conservation in Heart Failure Patients With SGLT-2 Inhibitor Treatment) a single-center, double-blind, randomized, placebo-controlled trial, which patients with chronic heart...
We have reported earlier that a high salt intake triggered an aestivation-like natriuretic-ureotelic body water conservation response lowered muscle mass and increased blood pressure. Here, we tested the hypothesis similar adaptive occurs in experimental chronic renal failure.In four subsequent experiments Sprague Dawley rats, used surgical 5/6 reduction (5/6 Nx) to induce failure. studied solute excretion 24-hour metabolic cage experiments, pressure by radiotelemetry, adjustment liver...
The kidney is easily affected by aging-associated changes, including glomerulosclerosis, tubular atrophy, and interstitial fibrosis. Particularly, renal tubulointerstitial fibrosis a final common pathway in most forms of progressive disease. Angiotensin II type 1 receptor (AT1R)-associated protein (ATRAP), which was originally identified as molecule that binds to AT1R, highly expressed the kidney. Previously, we have shown ATRAP suppresses hyperactivation AT1R signaling, but does not affect...
The relation between B2 cells and commensal microbes during atherosclerosis remains largely unexplored. Here we show that under hyperlipidemic conditions intestinal microbiota resulted in recruitment ectopic activation of perivascular adipose tissue, followed by an increase circulating IgG, promoting disease development. In contrast, disruption the a broad-spectrum antibiotic cocktail (AVNM) led to attenuation suppressing cells, despite persistence serum lipid abnormalities. Furthermore,...
The intradermal (ID) route for vaccination represents an effective alternative to subcutaneous (SC)/intramuscular administration induce protective immunity. However, a critical issue associated with ID is the precise delivery of solution in upper dermis, which ensures enhanced immunity.We fabricated hollow microneedle unit made poly-glycolic acid by injection molding and bonding, created dedicated prototype injector. To ensure solution, injected site was macroscopically microscopically...
Abnormal angiogenesis plays a major role in the development of early stage diabetic nephropathy. Vascular endothelial growth factor (VEGF) is classical proangiogenic that regulates abnormal glomerular linked to hypertrophy Leucine-rich α -2-glycoprotein-1 (LRG1) was recently reported as novel expressed cells and promotes by modulating transforming factor- β signaling pathway. However, pathophysiology LRG1 nephropathy remains largely unknown. In present study, we investigated intrarenal...
The interaction between atherosclerosis and commensal microbes through leaky gut syndrome (LGS), which is characterized by impaired intestinal permeability the introduction of undesired pathogens into body, has not been fully elucidated. Our aim was to investigate potential role a ClC-2 chloride channel activator, lubiprostone, reported have beneficial effects on LGS, in development apolipoprotein E–deficient (ApoE-/-) mice. After 15-week feeding period Western diet (WD), ApoE-/- mice were...
Atherosclerotic diseases, such as coronary artery disease and peripheral disease, are systemic disorders among the leading causes of mortality morbidity throughout world. However, exact pathophysiological mechanisms underlying development atherosclerosis remain unknown; currently, is thought to involve an inflammatory process. Systemic reactions accumulation immune cells in atherosclerotic lesions situ considered essential. We have comprehensively analyzed autoantibodies patients with by...
Abstract Enhancement of AT1 receptor-associated protein (ATRAP) in adipose tissue improves high fat diet (HFD)-induced visceral obesity and insulin resistance, suppresses oxidative stress. However, HFD loading is not a direct stimulatory factor for receptor. In the present study, we investigated effect chronic, low-dose angiotensin II (Ang II) stimulation on glucose lipid metabolism mice functional role ATRAP. ATRAP expression was higher (5–10-fold) skeletal muscle (approximately 1.6-fold)...
Abstract Epithelial sodium channels (ENaCs) play critical roles in the maintenance of fluid and electrolyte homeostasis their genetic abnormalities cause one type hereditary salt-sensitive hypertension, Liddle syndrome. As we reported previously, both human rodent Nedd4L/Nedd4-2 showed molecular diversity, with without a C2 domain N-terminal. isoforms are hypothesized to be related closely ubiquitination ENaCs. We generated Nedd4-2 knockout mice. demonstrate here that loss isoform causes...
Abstract The underlying pathogenesis of chronic kidney disease involves an activated renin-angiotensin system and systemic inflammation which ultimately develop renal injury. Rikkunshito (RKT) has been reported to exert anti-fibrotic anti-inflammatory effects through enhancement ghrelin signaling pathway. In this study, we investigated the RKT on fibrosis in angiotensin II (Ang II)-induced injury model. Ang II-infused mice exhibited hypertension, cardiac hypertrophy, increases blood urea...
Renin belongs to a family of aspartyl proteases and is the rate-limiting enzyme in synthesis potent vasoactive peptide angiotensin II. Processing renal renin has been extensively investigated juxtaglomerular granular cells, which prorenin active are present secretory condensed granules. Previous studies demonstrated alternative transcription rat adrenal glands. Different reported novel intracellular forms deduced from 5′ variants derived mRNA both mice humans. Comprehensive detailed...
Background We have previously shown that ATRAP (angiotensin II receptor-associated protein; Agtrap) interacts with AT1R type 1 receptor) and promotes constitutive internalization of AT 1R so as to inhibit hyperactivation its downstream signaling. In response angiotensin , systemic deficiency exacerbates -mediated hypertension via renal tubular 1R. Although expression is abundant in proximal tubules, little known about the actual function tubule angiotensin-mediated hypertension. Methods...
Acute kidney injury (AKI) is a global public health concern with high mortality and morbidity. In ischemic reperfusion (IRI), main cause of AKI, the brush border membrane S3 proximal tubules (PT) lost to tubular lumen. How injured reconstitute lipids during renal recovery not known. Here, we identified Mfsd2a, sodium-dependent lysophosphatidylcholine (LPC) transporter, be expressed specifically in basolateral PT. Using an vivo activity probe for transport was found specific Mice...
Epithelial sodium channels (ENaC) are ion transporters in the aldosterone-sensitive distal nephron that play an important role reabsorption terminal nephron. Our study of inbred C57Bl6/J mice given a high-sodium diet showed increased ENaC expression accompanied by tubular renin activation on qRT-PCR laser-captured tubule specimens and Western blotting membrane proteins, despite inhibition aldosterone. Treatment with angiotensin-converting-enzyme inhibitor (ACEI) or angiotensin receptor...