A5028758045- Glioma Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cancer Cells and Metastasis
- Cancer, Stress, Anesthesia, and Immune Response
- Chemokine receptors and signaling
- Cancer Immunotherapy and Biomarkers
- Extracellular vesicles in disease
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Nanoplatforms for cancer theranostics
- Aluminum toxicity and tolerance in plants and animals
- CAR-T cell therapy research
- Phagocytosis and Immune Regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Mesenchymal stem cell research
- Neuroblastoma Research and Treatments
- Inflammatory mediators and NSAID effects
- Brain Metastases and Treatment
- Hedgehog Signaling Pathway Studies
- Monoclonal and Polyclonal Antibodies Research
Lund University
2015-2025
KU Leuven
2011
Abstract The DNA repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) is commonly overexpressed in cancers and implicated the development of chemoresistance. use drugs inhibiting MGMT has been hindered by their haematologic toxicity inefficiency. As a different strategy to inhibit we investigated cellular regulators expression multiple cancers. Here show significant correlation between Wnt signalling with origin confirm findings bioinformatic analysis immunofluorescence. We...
Bone marrow-derived multipotent mesenchymal stroma cells (MSCs) have emerged as cellular vectors for gene therapy of solid cancers. We implanted enhanced green fluorescent protein-expressing rat MSCs directly into malignant gliomas to address their migratory capacity, phenotype, and effects on tumor neovascularization animal survival. A single intratumoral injection infiltrated the majority invasive glioma extensions (72 +/- 14%) a substantial fraction distant microsatellites (32 6%). MSC...
Medulloblastomas are the most common malignant brain tumors in children. They express high levels of COX-2 and produce PGE2, which stimulates tumor cell proliferation. Human cytomegalovirus (HCMV) is prevalent human population encodes proteins that provide immune evasion strategies promote oncogenic transformation oncomodulation. In particular, HCMV induces expression; STAT3 phosphorylation; production vascular endothelial growth factor, IL-6; formation vivo. Here, we show a large proportion...
Glioblastoma multiforme (GBM) is the most aggressive form of brain tumor. In general, tumor growth requires disruption tissue microenvironment, yet how this affects glioma progression unknown. We studied program death-ligand (PD-L)1 in neurons and gliomas tumors from GBM patients associated findings with clinical outcome. Remarkably, we found that upregulation PD-L1 by tumor-adjacent (TABT) positively patient survival, whereas lack neuronal expression was high unfavorable prognosis. To...
Abstract Aluminium-based adjuvants (ABA) are the predominant used in human vaccinations. While a consensus is yet to be reached on aetiology of biological activities ABA several studies have identified shape, crystallinity and size as critical factors affecting their adjuvanticity. In spite recent advances, fate following administration remains unclear. Few if any demonstrated unequivocal presence intracellular ABA. Herein we demonstrate for first time identification within monocytic T...
Primary brain tumors, in particular glioblastoma (GBM), remain among the most challenging cancers. Like malignant GBM is characterized by hypoxic stress that triggers paracrine, adaptive responses, such as angiogenesis and macrophage recruitment, rescuing cancer cells from metabolic catastrophe conventional oncological treatments. The unmet need of strategies to efficiently target tumor "stressness" represents a strong clinical motivation better understand underlying mechanisms adaptation....
Abstract Background Glioblastoma, IDH wildtype is the most common primary malignant brain tumor in adults. Despite best available treatment, prognosis remains poor. Current standard therapy consists of surgical removal followed by radiotherapy and chemotherapy with alkylating agent temozolomide. Antisecretory factor (AF), an endogenous protein, may potentiate effect temozolomide alleviate cerebral edema. Salovum® egg-yolk powder enriched for AF classified as a medical food European Union....
Abstract Several chemotherapeutic drugs are now considered to exert anti-tumour effects, by inducing an immune-promoting inflammatory response. Cisplatin is a potent agent used in standard medulloblastoma but not glioblastoma protocols. There no clear explanation for the differences clinical efficacy of cisplatin between medulloblastomas and glioblastomas, despite fact that effective vitro against latter. Systemic toxicity often dose limiting could tentatively be reduced intratumoral...
Significance Here we describe and utilize a model of medulloblastoma, malignancy accounting for 20% all childhood brain cancers. We used iPS-derived neural stem cells with familial mutation causing aberrant SHH signaling. show that these cells, when transplanted into mouse cerebellum, form tumors mimics SHH-driven demonstrating the development cancer from healthy in vivo. Our results reprogramming somatic carrying mutations can be to initiation progression cancer.
Immunotherapy has shown effectiveness against experimental malignant brain tumors, but the clinical results have been less convincing most likely due to immunosuppression. Prostaglandin E2 (PGE2 ) is key immunosuppressive product of cyclooxygenase-2 (COX-2) and increased levels PGE2 COX-2 in several tumor types, including tumors. In current study, we report enhanced cure rate mice with established mouse GL261 tumors when immunized granulocyte macrophage-colony stimulating factor (GM-CSF)...
Glioblastomas (GBM) are therapy-resistant tumors with a profoundly immunosuppressive tumor microenvironment. Chemotherapy has shown limited efficacy against GBM. Systemic delivery of chemotherapeutic drugs is hampered by the difficulty achieving intratumoral levels as systemic toxicity dose-limiting factor. Although some its effects might be mediated immune reactivity, chemotherapy can also inhibit induced or spontaneous antitumor reactivity. Convection-enhanced temozolomide (CED-TMZ)...
The current study was designed to critically evaluate the notion that cancer stem cell (CSC)-like cells constitute a subpopulation of within experimental gliomas. Virtually all N29 and N32 rat glioma models homogenously expressed CD133, stem/progenitor marker nestin as well neural lineage markers glial fibrillary acidic protein, betaIII-tubulin, CNPase in vitro. phenotype largely retained on exposure conditions promoting differentiation vitro after intracranial implantation tumor into...
Glioblastoma presents a formidable clinical challenge because of its complex microenvironment. Here, we characterized tumor-associated foam cells (TAFs), type lipid droplet–loaded macrophage, in human glioblastoma. Through extensive analyses patient tumors, together with vitro and vivo investigations, found that TAFs exhibit distinct protumorigenic characteristics related to hypoxia, mesenchymal transition, angiogenesis, impaired phagocytosis, their presence correlates worse outcomes for...
We were the first to demonstrate that combined immunotherapy with GM-CSF producing GL261 cells and recombinant IFNgamma of preestablished gliomas could cure 90% immunized mice. To extend these findings uncover underlying mechanisms, ensuing experiments undertaken. hypothesized immunizations combining both systemically would increase number immature myeloid cells, which then mature differentiate into dendritic (DCs) macrophages, thereby augmenting tumor antigen presentation T-cell activation....
Glioblastoma are highly aggressive tumors with an average survival time of 12 months currently available treatment. We have previously shown that specific embryonic neural progenitor cells (NPC) the potential to target glioma growth in CNS rats. The cell treatment can cure approximately 40% animals malignant gliomas no trace a tumor burden 6 after finishing experiment. Furthermore, NPCs been respond signals from environment resulting migration towards tumor. Based on these results we wanted...