A5100402811- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Gallbladder and Bile Duct Disorders
- Circular RNAs in diseases
- RNA modifications and cancer
- MicroRNA in disease regulation
- Pediatric Hepatobiliary Diseases and Treatments
- Gastric Cancer Management and Outcomes
- Metastasis and carcinoma case studies
- Pancreatic and Hepatic Oncology Research
- Viral-associated cancers and disorders
- Oral and Maxillofacial Pathology
- Peptidase Inhibition and Analysis
- Liver physiology and pathology
- Tissue Engineering and Regenerative Medicine
- PI3K/AKT/mTOR signaling in cancer
- Cancer Mechanisms and Therapy
- Extracellular vesicles in disease
- Vascular Tumors and Angiosarcomas
- Natural Compounds in Disease Treatment
- Bioactive Compounds and Antitumor Agents
- Xenotransplantation and immune response
- Organ Transplantation Techniques and Outcomes
- CNS Lymphoma Diagnosis and Treatment
Eastern Hepatobiliary Surgery Hospital
2013-2024
Second Military Medical University
2013-2024
State Council of the People's Republic of China
2018
Air Force Medical University
2016
Abstract Background Circular RNAs (circRNAs) have recently been identified as potential functional modulators of the cellular physiology processes. The study aims to uncover clinical value and driving molecular mechanisms circRNAs in gallbladder cancer (GBC). Patients methods We performed RNA sequencing from four GBC paired adjacent normal tissues analyze circRNA candidates. Quantitative real-time polymerase chain reaction (QRT-PCR) was used measure circFOXP1 expression 40 patient tissue...
Abstract Long noncoding RNAs (lncRNA) are being implicated in the development of many cancers. Here, we report discovery a critical role for lncRNA GCASPC determining progression gallbladder cancer. Differentially expressed lncRNAs and mRNAs between cancer specimens paired adjacent nontumor tissues from five patients were identified validated by an expression microarray analysis. Quantitative real-time PCR was used to measure levels 42 patients, confirmed further separate cohort 89 patients....
Gallbladder carcinoma (GBC) is the most common biliary tract malignancy with lowest survival rate, primarily arising from chronic inflammation. To better characterize progression inflammation to cancer metastasis, we performed single-cell RNA sequencing across samples of 6 cholecystitis, 12 treatment-naive GBCs, and matched metastases. Benign epithelial cells inflamed gallbladders displayed resting, immune-regulating, gastrointestinal metaplastic phenotypes. A small amount PLA2G2A+ copy...
Background/Aims: MicroRNAs (miRNAs) play critical roles during carcinogenesis and cancer progression. Down-regulation of miR-204 has been frequently observed in various cancers. In this study, we investigated the mechanisms human intrahepatic cholangiocarcinoma (ICC). Methods: The relative expression ICC tissues cell lines was monitored by qRT-PCR. Effects were studied HuH28 HuCCT1, cells analyzed for proliferation, migration invasion. Expression levels target gene Slug EMT markers...
Tissue sampling of biliary tract carcinomas (BTCs) for molecular characterization is challenging. The aim this study was to investigate the possibility identifying individual actionable mutations derived from bile cell‑free DNA (cfDNA) using targeted deep sequencing. Ten BTC patients, four with gallbladder and six cholangiocarcinomas, were enrolled in present study. Using sequencing a panel 150 tumor‑related genes, paired cfDNA tumor analyzed mutational variants individually then compared....
Transforming growth factor β (TGF-β) plays important roles in tumor metastasis by regulating miRNAs expression. miR-182 is an molecule the regulation of cancer progression. The aim study to assess role TGF-β-induced metastasis. In present study, we found that levels are significantly upregulated GBC tissues compared with normal controls, and expression remarkably increased primary tumors subsequently metastasized, when those did not metastasize. TGF-β induces cells, overexpression promotes...
Background Axl is a receptor tyrosine kinase which plays an important role in multiple human malignancies. Design The expression was examined several hepatocellular carcinoma(HCC) cell lines, paired tumor and nontumorous samples. Then, we growth curve, apoptosis migration SMMC-7721 cells over-expressed with or siRNA against Axl, respectively. Finally, the prognostic value of investigated prospective cohort 246 consecutive HCC patients undergoing curative hepatoectomy. Results We found...
In cell-based therapies for liver injuries, the clinical outcomes are closely related to surrounding microenvironment of transplanted bone marrow mesenchymal stem cells (BM-MSCs). However, whether liver-specific ECM (L-ECM), as one major signals, could regulate therapeutic effect BM-MSCs through changing their biological characteristics is unclear. This study aimed investigate hepatogenicity and underlying mechanism L-ECM well its potential regulative role in MSC-based recovery. was prepared...
The role of lncRNAs in gallbladder cancer (GBC) remains poorly understood. In this study, we explored the function functional intergenic repeating RNA element (FIRRE) GBC. Whole transcriptome resequencing was performed three pairs GBC tissues and adjacent non-tumor tissues. lncRNA FIRRE expression verified by real-time PCR. evaluated experiments vitro vivo. mechanism investigated via fluorescent situ hybridization, pull-down, dual luciferase reporter assays, immunoprecipitation. level...
The aim of this study was to investigate histopathologic prognostic factors in patients with intrahepatic cholangiocarcinoma (ICC) whose tumors were resected determine the optimal surgical strategies.One hundred and two ICC who underwent laparotomy from July 1998 December 2000 followed up successfully. Histopathologic variables selected for univariate multivariate analyses evaluate their influence on outcome.The 1-, 3-, 5-year survival rates after surgery 56.9%, 25.5%, 16.9%, respectively....
BACKGROUND: Currently, the most effective treatment for intrahepatic cholangiocarcinoma (ICC) is complete hepatic tumour excision. OBJECTIVE: To identify clinical parameters associated with survival duration ICC patients following hepatectomy, and to construct a mathematical model predicting duration. METHODS: Demographic data variables 102 diagnosed ICC, who underwent exploratory laparotomy at single centre from July 1998 December 2000 were followed an average of 24 months, collected in...
The present study aimed to investigate the effects of microRNA expression in intrahepatic cholangiocarcinoma (ICC). It was identified that microRNA‑26b‑5p (miR‑26b‑5p) downregulated ICC tissues compared with matched adjacent non‑tumor tissues. Furthermore, miR‑26b‑5p metastatic tumor and invasive cell line subpopulations non‑metastatic tissue parental cells. In vitro studies demonstrated transfection an mimic inhibited proliferation, migration invasion RBE HCCC‑9810 cells, whereas inhibitor...
Cell-free DNA (cfDNA) exists in various types of bodily fluids, including plasma, urine, bile, and others. Bile cfDNA could serve as a promising liquid biopsy for biliary tract cancer (BTC) patients, bile directly contacts tumors the system. However, there is no commercial kit or widely acknowledged method extraction. In this study, we established silica-membrane-based method, namely 3D-BCF, isolation, exhibiting effective recovery fragments spike-in assay. We then compared 3D-BCF with four...
Bile cell-free DNA (cfDNA) has been reported as a promising liquid biopsy tool for cholangiocarcinoma (CCA), however, the whole-genome mutation landscape and structural variants (SVs) of bile cfDNA remains unknown. Here we performed sequencing on analyzed correlation between characteristics clinical prognosis. TP53 KRAS were most frequently mutated genes, RTK/RAS, homologous recombination (HR), HIPPO top three pathways containing gene mutations. Ten overlapping putative driver genes found in...
The aim of this study was to investigate the effects verapamil on pharmacokinetics pristimerin in rats. A sensitive and reliable LC-MS/MS method developed applied for determination rat plasma. orally administered with without pretreatment investigated. results indicated that, when rats were pretreated verapamil, peak concentration increased from 189.13 277.53 ng/mL (46.7%), AUC0-t by approximately 82.0%. Additionally, absorption metabolic stability investigated using Caco-2 cell transwell...
The prognostic benefits and safety of extended lymphadenectomy for hilar cholangiocarcinoma remain uncertain. available evidence is still insufficient concerning its retrospective aspect. aim this study to explore the clinical effect compared regional in patients with cholangiocarcinoma. Relay-HC trial a prospective, multicenter, randomized controlled trial. Seven hundred thirty-four eligible resectable perihilar across 15 tertiary hospitals China will be randomly assigned (1:1) receive...
Background: Intrahepatic cholangiocarcinoma has heterogeneous outcomes after resection. There remains a need for broadly applicable recurrence-specific tool offering precise evaluation on curativeness of
To study clinical features, imaging findings, and prognosis of hepatic epithelioid hemangioendothelioma (HEHE) in Eastern Hepatobiliary Surgical Hospital to improve the level recognition preoperative diagnosis HEHE. This is a retrospective study. The was conducted at Shanghai, China from 1999 2012. Clinical data 15 pathology-confirmed HEHE patients admitted our hospital 1999-2010 were collected analyzed retrospectively. Besides 7 cases abdominal discomfort, this cohort does not show common...
Objective. In this study, α-Gal epitope-deficient (GGTA1 knockout (GTKO)) mice were used to assess the immunological risks of xenogeneic dural patch by comparing with raw material. Methods. The (T2) was prepared from bovine pericardium (T1, material) through decellularization and carboxymethyl chitosan (CMCS) coating. Transmission electron microscopy (TEM) scanning (SEM) characterize collagen fibers surface microstructural changes in T1 T2 samples. remnant epitopes DNA implants detected...