A5101578188- Bone and Dental Protein Studies
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Connective Tissue Growth Factor Research
- Crystallization and Solubility Studies
- DNA Repair Mechanisms
- Metal-Organic Frameworks: Synthesis and Applications
- X-ray Diffraction in Crystallography
- Pharmaceutical and Antibiotic Environmental Impacts
- Immune cells in cancer
- Connective tissue disorders research
- Redox biology and oxidative stress
- Carcinogens and Genotoxicity Assessment
- Fluoride Effects and Removal
- Crystal structures of chemical compounds
- Synthesis and Characterization of Heterocyclic Compounds
- Listeria monocytogenes in Food Safety
- Advanced Photocatalysis Techniques
- Salmonella and Campylobacter epidemiology
- Toxic Organic Pollutants Impact
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Ubiquitin and proteasome pathways
Tianjin Normal University
2024-2025
West China Medical Center of Sichuan University
2020-2025
ShanghaiTech University
2020-2024
Yunnan Agricultural University
2024
Changsha Medical University
2008-2023
Suizhou Central Hospital
2023
Sichuan University
2013-2023
Tokyo Metropolitan University
2023
Huazhong University of Science and Technology
2008-2022
Center for Excellence in Molecular Cell Science
2020-2021
Neutrophil extracellular traps (NETs) are important pieces of equipment for neutrophils. Excess NETs play promoting roles in cancer-associated thrombosis (CAT). Therefore, directing formation is a promising therapeutic strategy and related diseases. Diosmetin, an antioxidant flavonoid derived from dietary sources, might be involved CAT. Firstly, the tests cell-free DNA Immunofluorescence were applied to evaluate levels Luminol-based chemiluminescence DCFH-DA probe used detect reactive oxygen...
Classical tooth development theory suggests that dental papilla cells (DPCs) are the precursor of odontoblasts, which responsible for dentin development. However, our previous studies have indicated follicle (DFCs) can differentiate into odontoblasts. To further understanding development, and differences in dentinogenesis between DFCs DPCs, odontogenic differentiation DPCs was characterized vitro vivo. were individually combined with treated matrix (TDM) before they subcutaneously implanted...
This study aims to demonstrate the antibiotic resistance and its association with disinfectant heavy metal in 152 Salmonella isolates recovered from retail foods of animal origins. Susceptibility testing demonstrated that 92.8% were resistant at least one antibiotic, was highest oxytetracycline (80.9%), followed by trimethoprim (64.5%), amoxicillin (28.9%), ampicillin (28.3%), levofloxacin (21.7%), ciprofloxacin (16.4%), gentamicin (10.5%), respectively. The blaTEM tetA genes (44.7%)...
To demonstrate the resistance of antibiotics and disinfectants to Escherichia coli isolates, 255 E. strains were isolated from 328 retail meat samples in this study. Susceptibility testing results showed that 85.5% isolates resistant at least one antibiotic drug. The highest sulfamethoxazole (61.6%), followed by tetracycline (61.2%), ampicillin (48.2%), cefalotin (29.8%), kanamycin (22.4%). minimum inhibitory concentrations cetyltrimethylammonium bromide, N,N-didecyl-N,N-dimethylammonium...
We studied the relationship between 2,2,4,4-tetrabromodiphenyl ether (PBDE-47) and oxidative DNA damage as well mode of interaction PBDE-47 2,2,4,4,5,5-hexachlorobiphenyl (PCB153) by incubating SH-SY5Y cells in four doses (0, 1, 5, 10μM) and/or 5μM PCB153 100μM NAC (N-acetylcysteine) for 24 h. Results showed that reactive oxygen species (ROS) production + 10μM groups were significantly higher than control group (p < 0.05). strand breakage 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels increased...
A novel protocol for synthesizing N-alkyl indoles from readily available N-nitrosoanilines and iodonium ylides through the rhodium(III)-catalyzed C-H bond activation/intramolecular cyclization reaction has been described. This strategy employs nitroso as a traceless directing group. The transformation features powerful reactivity, tolerates various functional groups, proceeds with moderate yields under mild conditions, providing straightforward approach to access structurally diverse...
Abstract The mechanisms underlying fluoride‐induced apoptosis in neurons still remain unknown. To investigate apoptosis, caspase‐3 activity, and mRNA expression of Fas, Fas‐L, caspases (‐3 ‐8) induced by fluoride, human neuroblastoma (SH‐SY5Y) cells were incubated with 0, 20, 40, 80 mg/L sodium fluoride (NaF) for 24 h vitro . data show that cell viability the 40 groups significantly lower than control group. percentages markedly higher those group, they increased increase concentration....
The study aims to demonstrate the antimicrobial and disinfectant resistance phenotypes genotypes of Escherichia coli isolates obtained from giant pandas (Ailuropoda melanoleuca). Antimicrobial testing was performed according standard disk diffusion method. minimal inhibitory concentrations (MICs) disinfectants were determined using agar dilution All screened for presence genes further analysed genetic relatedness by pulse‐field gel electrophoresis (PFGE). Results showed that 46·6% resistant...
Article13 August 2020Open Access Source DataTransparent process Dynamics of Staphylococcus aureus Cas9 in DNA target Association and Dissociation Siqi Zhang School Life Science Technology, ShanghaiTech University, Shanghai, China Shanghai Institute Biochemistry Cell Biology, Chinese Academy Sciences, University Beijing, Search for more papers by this author Qian orcid.org/0000-0002-9187-4277 Xi-Miao Hou College Northwest A&F Yangling, Lijuan Guo Fangzhu Wang State Key Laboratory Reproductive...
Abstract Bloom syndrome protein (BLM) is a conserved RecQ family helicase involved in the maintenance of genome stability. BLM has been widely recognized as “caretaker” that processes structured DNA. In contrast, our knowledge how behaves on single‐stranded (ss) DNA still limited. Here, we demonstrate possesses intrinsic ability for phase separation and can co‐phase separate with ssDNA to form dynamically arrested protein/ssDNA co‐condensates. The introduction ATP potentiates capability...
Abstract The mechanisms underlying the neurotoxicology of endemic fluorosis still remain obscure. To explore lactate dehydrogenase (LDH) leakage, intracellular Ca 2+ concentration ([Ca ] i ) and reactive oxygen species (ROS) production induced by fluoride, human neuroblastoma (SH‐SY5Y) cells were incubated with sodium fluoride (NaF, 20, 40, 80 mg/L) for 24 h, 40 mg/L NaF 3, 6, 12, 18, N ‐acetyl‐ L ‐cysteine (NAC), ethyleneglycol‐bis‐(β‐aminoethyl ether)‐ , ′, ′‐tetraacetic acid (EGTA),...
Abstract The tripartite ParABS system mediates chromosome segregation in a wide range of bacteria. Dimeric ParB was proposed to nucleate on parS sites and spread neighboring DNA. However, how properly distributed dimers further compact chromosomal DNA into higher-order nucleoprotein complex for partitioning remains poorly understood. Here, using single-molecule approach, we show that tens Bacillus subtilis (Spo0J) proteins can stochastically multimerize stably bind nonspecific introduction...
Osteogenesis and senescence of BMSCs play great roles in age-related bone loss. However, the causes these dysfunctions remain unclear. In this study, we identified a differentially expressed ASPH gene middle-aged elderly-aged groups which were obtained from GSE35955. Subsequent analysis various databases, such as TCGA, GTEx CCLE, revealed that had positive correlations with several osteogenic markers. The depletion mouse Asph suppressed capacity differentiation marrow mesenchymal stem cells...
Single-stranded guanine-rich RNA sequences have a propensity to fold into compact G-quadruplexes (RG4s). The conformational transitions of these molecules provide an important way regulate their biological functions. Here, we examined the stability and conformation RG4-forming sequence identified near end human telomerase RNA. We found that proximal single-stranded (ss) significantly impairs RG4 at physiological K+ concentrations, resulting in reduced rupture force ∼ 24.4 pN easier...
Wisp3 gene mutation was shown to cause spondyloepiphyseal dysplasia tarda with progressive arthropathy (SRDT-PA), but the underlying mechanism is not clear. To clarify this mechanism, we constructed wild and mutated expression vectors transfected into human chondrocytes lines C-20/A4; proteins subcellular localization, cell proliferation, apoptosis, Wisp3-mediated were determined, dynamic secretion of collagen in analyzed by (14)C-proline incorporation experiment. Mutated protein increased...
Located in the southeastern China and northern Vietnam, Red River is an important international trans-boundary river that has experienced rapid deforestation environmental changes over past decades. We conducted attribution analysis of impacts various on streamflow sediment load. The contribution reclassified to total change load was separated. Land cover based climate-induced human-induced indicators were defined. found land main factor affecting Changes more pronounced dry season than wet...
Ethylene (E) copolymers with isoprene (IP) possessing higher glass-transition temperatures (Tg values, −7.1 to 29.2 °C) than those prepared by the reported catalysts below −18 have been in copolymerization using half-titanocene containing phenoxide ligand, Cp′TiCl2(O-2,6-iPr2-4-R-C6H2) [Cp′ = C5Me5 (Cp*), R H (1), SiEt3 (2)]. Their microstructural analysis NMR spectra revealed that contained cyclopentane (major) and cyclohexane units, formed cyclization after IP subsequent ethylene...
Abstract The tripartite ParABS system mediates chromosome segregation in the majority of bacterial species. Typically, DNA-bound ParB proteins around parS sites condense chromosomal DNA into a higher-order multimeric nucleoprotein complex for ParA-driven partition. Despite extensive studies, molecular mechanism underlying dynamic assembly partition remains unclear. Herein, we demonstrate that Bacillus subtilis (Spo0J), through multimerization its N-terminal domain, forms phase-separated...