A5006993257- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Hepatitis B Virus Studies
- Hemoglobinopathies and Related Disorders
- Iron Metabolism and Disorders
- HIV/AIDS drug development and treatment
- Hepatitis Viruses Studies and Epidemiology
- Dermatologic Treatments and Research
- Biochemical and Molecular Research
- Immunotherapy and Immune Responses
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- HIV Research and Treatment
- Glaucoma and retinal disorders
- Facial Rejuvenation and Surgery Techniques
- Genetic Neurodegenerative Diseases
- Folate and B Vitamins Research
- Viral gastroenteritis research and epidemiology
- Genomics and Rare Diseases
- Cancer-related molecular mechanisms research
- Nitric Oxide and Endothelin Effects
- Corneal surgery and disorders
- Hemoglobin structure and function
- Liver Diseases and Immunity
- Ocular Disorders and Treatments
Chinese Academy of Sciences
2008-2025
Shanghai Children's Medical Center
2024-2025
Shanghai Jiao Tong University
2019-2025
Shenzhen Institutes of Advanced Technology
2025
Central South University
2008-2024
King's College London
2022-2024
Second Xiangya Hospital of Central South University
2024
Sun Yat-sen University
2021-2023
Sun Yat-sen Memorial Hospital
2021-2023
PharmacoGenetics (China)
2022
Objective To explore meibomian gland dysfunction (MGD) may determine the severity of dry eye conditions in visual display terminal (VDT) workers. Methodology Prospective, case-control study carried out China.106 eyes 53 patients (VDT work time >4 hour per day) were recruited as Long VDT group; 80 40 control subjects ≤4 served Short group. A questionnaire Ocular Surface Disease Index (OSDI) and multiple tests performed. Three tests: tear film breakup (BUT), corneal fluorescein staining,...
Hemojuvelin (HJV) is a glycosylphosphatidylinositol-linked protein and binds both bone morphogenic proteins (BMPs) neogenin. Cellular HJV acts as BMP co-receptor to enhance the transcription of hepcidin, key iron regulatory hormone secreted predominantly by liver hepatocytes. In this study we characterized role neogenin in HJV-regulated hepcidin expression. Both were expressed Knockdown decreased BMP4-induced mRNA levels 16-fold HJV-expressing HepG2 cells but only about 2-fold transfected...
Hepatitis E virus (HEV) is a serious public health problem. The commonly used tests that are specific for current HEV infection diagnosis include the detection of anti-HEV IgM and RNA. Here, we report an improved enzyme-linked immunosorbent assay (ELISA) method antigen with linear range equivalent to 6.3 × 10(3) 9.2 10(5) RNA copies per ml. monoclonal antibody (MAb) 12F12, high-ability MAb binds virus, was selected as capture from panel 95 MAbs. positive period antigenemia in infected...
Chaihu-Shugan-San (CSS) is a traditional botanical drug formula often prescribed to treat depression in oriental countries, but its pharmacotherapeutic mechanism remains unknown. It was recently reported that CSS alters the composition of intestinal microflora and related metabolites such as bile acids (BAs). Since affects physiological functions brain through gut-microbiota-brain axis, herein we investigated whether altered BA levels, gut microflora, depression-like symptoms chronic...
Tear film breakup time (TFBUT) is an essential parameter used to diagnose dry eye disease (DED). However, a robust method for examining TFBUT in murine models has yet be established. We invented innovative device, namely, the "Smart Eye Camera", which addresses several problems associated with existing methods and capable of evaluating DED model. compared images taken by devices Smart Camera graft-versus-host disease-related observed that quality obtained were sufficient practical use....
Hemojuvelin is a recently identified iron-regulatory protein that plays an important role in affecting the expression of hepcidin, key iron regulatory hormone. Although underlying mechanism this process not clear, several hemojuvelin-binding proteins, including cell surface receptor neogenin and bone morphogenetic (BMP) cytokines, have been identified. The ectodomain composed four immunoglobulin-like (Ig) domains followed by six fibronectin type III-like (FNIII) domains. Here we report...
Rad9 is conserved from yeast to humans and plays roles in DNA repair (homologous recombination repair, base-pair excision repair) cell cycle checkpoint controls. It has not previously been reported whether involved mismatch (MMR). In this study, we have demonstrated that both human mouse interacts physically with the MMR protein MLH1. Disruption of interaction by a single-point mutation leads significantly reduced activity. This disruption does affect S/M control first round G(2)/M control,...
HFE2 (hemochromatosis type 2 gene) is highly expressed in skeletal muscle and liver hepatocytes. Its encoded protein, hemojuvelin (HJV), a co-receptor for the bone morphogenetic proteins 4 (BMP2 BMP4) enhances BMP-induced hepcidin expression. Hepcidin central iron regulatory hormone predominantly secreted from HJV also binds neogenin, membrane protein widely many tissues. Neogenin required processing release of cells. The role that neogenin plays trafficking was investigated, using HepG2...
Abstract Pterygium is a very common disease in an eye clinic characterized by benign proliferation of local conjunctiva that often crosses the limber cornea and extends into corneal surface. Variety studies has showed pterygium able to result ocular discomfort change surface environment, such as dry eye. However, link between abnormal tear film function controversial. Meibomian gland dysfunction (MGD) cause but neglected, which may be missing pterygium. In this study, our data firstly...
Chronic graft-versus-host disease (cGVHD) is one of the most frequent complications experienced after allogeneic hematopoietic stem cell transplantation. Reportedly, dysbiosis and severe damage to microbiome are also closely associated with GVHD. Herein, we aimed elucidate positive negative effects administration various antibiotics in a murine model cGVHD. For bone marrow transplantation (allo-BMT), from B10.D2 mice were transplanted BALB/c induce The cGVHD orally administered ampicillin,...
Uveal melanoma (UVM) is the most common primary ocular malignancy in adults and involves several types of regulated cell death. Cuproptosis a novel method regulating death by binding lipoylated TCA cycle proteins. There still no research on relationship between cuproptosis-related genes (CRGs) UVM. Here, we aimed to develop prognostic CRG signature for After was constructed, determined immune infiltration, bioinformatics analysis experimental validation. Finally, three-gene (CRTG) which...
Regulatory T cells (Tregs) are involved in the maintenance of immune homeostasis and regulation. Clinical trials on adoptive transfer Tregs have been ongoing for > 10 years. However, many unresolved issues remain production readymade Treg products selection patients. Hence, this study aimed to develop a method expand off-the-shelf derived from umbilical cord blood (UCB-Tregs) vitro without changing their phenotype inhibitory function. In addition, intended design an approach precisely select...
The ARCN1 gene encodes the delta subunit of coatomer protein complex I (COPI), which is essential for mediating transport from Golgi to endoplasmic reticulum. Variants in are associated with clinical features such as microcephaly, microretrognathia, intrauterine growth restriction, short rhizomelic stature, and developmental delays. We present a case patient exhibiting preterm birth, micrognathia, central precocious puberty. Whole-exome sequencing identified novel splice-site variant,...
Crystallographic studies of the catalytic core domain avian sarcoma virus integrase (ASV IN) have provided most detailed picture so far active site this enzyme, which belongs to an important class targets for designing drugs against AIDS. Recently, crystals inactive D64N mutant were obtained under conditions identical those used native enzyme. Data collected at different pH values and in presence divalent cations. also low ASV IN domain. In structures 6.0 below, as well all mutants, side...
Retroviral integration results in the stable and coordinated insertion of two termini linear viral DNA into host genome. An vitro concerted two-end reaction catalyzed by Moloney murine leukemia virus (M-MuLV) integrase (IN) was used to investigate binding coordination ends. Comparison strand transfer assays indicates that zinc is required for efficient utilizing plasmid as target. Complementation using a pair nonoverlapping domains, consisting HHCC domain core/C-terminal region, yielded...