A5100737515- Advanced biosensing and bioanalysis techniques
- Cancer Genomics and Diagnostics
- Electrocatalysts for Energy Conversion
- Genomics and Phylogenetic Studies
- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- Advanced Breast Cancer Therapies
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Melanoma and MAPK Pathways
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Entomopathogenic Microorganisms in Pest Control
- Advanced battery technologies research
- Fuel Cells and Related Materials
- Electrochemical Analysis and Applications
- Radical Photochemical Reactions
- CRISPR and Genetic Engineering
- Biosensors and Analytical Detection
- Molecular Sensors and Ion Detection
- Single-cell and spatial transcriptomics
- Microfluidic and Capillary Electrophoresis Applications
- Gene expression and cancer classification
- Plant tissue culture and regeneration
- Catalytic Processes in Materials Science
Changchun Institute of Applied Chemistry
2014-2025
Chinese Academy of Sciences
2012-2025
Shanghai Jiao Tong University
2017-2025
State Key Laboratory of Electroanalytical Chemistry
2025
Fujian Agriculture and Forestry University
2007-2025
Xiyuan Hospital
2025
China Academy of Chinese Medical Sciences
2025
Laboratoire de Synthèse Organique
2025
State Key Laboratory of Oncogene and Related Genes
2022-2024
The University of Texas MD Anderson Cancer Center
2014-2024
We present a colorimetric and ratiometric fluorescent probe Cy–N3 that exhibits selective response to H2S. The employs near-infrared cyanine as fluorophore, is equipped with an operating azide unit. It readily employed for assessing intracellular H2S levels, confocal imaging achieved successfully.
We have developed a new reversible fluorescence probe MPhSe–BOD for the redox cycle process between hypochlorous acid and hydrogen sulfide in solution living cells. Confocal microscopy imaging using RAW264.7 cell lines shows that has good membrane permeability, can monitor intracellular HClO/H2S cycles continuously.
Circulating tumor cells (CTCs) contain molecular information on the primary and can be used for predictive cancer diagnostics. Capturing rare live CTCs their quantification in whole blood remain technically challenging. Here we report an aptamer-trigger clamped hybridization chain reaction (atcHCR) method situ identification subsequent cloaking/decloaking of by porous DNA hydrogels. These decloaked were then cell analysis. In our design, a staple strand with aptamer-toehold biblocks...
The high occurrence of prostate cancer in men makes the prostate-specific antigen (PSA) screening test really important. More importantly, recurrence rate after radical prostatectomy is high, whereas traditional PSA immunoassay does not possess sufficient sensitivity for post-treatment detection. In these assays, uncontrolled and random orientation capture antibodies on surface largely reduces their activity. Here, by exploiting rapidly emerging DNA nanotechnology, we developed a...
Sensitive detection of cancer cells plays a critically important role in the early and metastasis. However, because circulating tumor are extremely rare peripheral blood, with high analytical sensitivity specificity remains challenging. Here, we have demonstrated simple, sensitive specific four cells, which is lower than cutoff value respect to correlation survival outcomes as well predictive metastatic disease clinical diagnostics. We re-engineered hybridization chain reaction (HCR)...
Abstract Protein-protein interactions are spatially regulated in living cells to realize high reaction efficiency, as seen naturally existing electron-transfer chains. Nevertheless, arrangement of chemical/biochemical components at the artificial device interfaces does not possess same level control. Here we report a tetrahedral DNA framework-enabled bulk enzyme heterojunction (BEH) strategy program multi-enzyme catalytic cascade interface electrochemical biosensors. The construction...
Understanding the behavior of biomolecules on nanointerface is critical in bioanalysis, which great challenge due to instability and difficulty control orientation loading density biomolecules. Here, we investigated thermodynamics kinetics DNA hybridization gold nanoparticle, with aim improve efficiency speed analysis. We achieved precise quantitative surface by applying a recently developed poly adenines (polyA)-based assembly strategy nanoparticles (DNA-AuNPs). PolyA served as an effective...
Cutaneous warts caused by human papillomavirus are benign proliferative lesions that occur at any ages in lives. Updated, comprehensive and systematic evidence-based guidelines to guide clinical practice urgently needed.We collaborated with multidisciplinary experts formulate this guideline based on evidences of already published literature, focusing 13 questions elected a panel experts. We adopted Grading Recommendations Assessment, Development Evaluation (GRADE) system form classification...
Abstract One major challenge in the design of highly multiplexed PCR primer sets is large number potential dimer species that grows quadratically with primers to be designed. Simultaneously, there are exponentially many choices for multiplex sequence selection, resulting systematic evaluation approaches being computationally intractable. Here, we present and experimentally validate Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE), a stochastic algorithm minimize...
Abstract Single‐atom catalysts (SACs) with high metal loadings are highly desirable but still challenging for large scale synthesis. Here we report a new technique named as dry‐solid‐electrochemical synthesis (DSES) general large‐scale of SACs in an energy‐conservation and environment‐friendly way. With it, series pure carbon‐supported (Platinum up to 35.0 wt %, Iridium 21.2 Ruthenium 20.4 % Iron 17.6 %) were obtained. Particularly, Pt SAC 23.9 remarkable oxygen reduction reaction (ORR)...
We present the design, synthesis, spectroscopy, and biological applications of PyBor, a new type fluorescent probe for peroxynitrite detection in aqueous solution living cells. The employs pyrene as fluorophore, is equipped with chemically responsive unit boronate. can selectively detect fluorimetric determination high-performance liquid chromatography analyses RAW264.7 cells intracellular free extracts. also study our to time dependent release from 3-morpholinylsydnonimine hydrochloride....
A novel (hetero)arylation of unactivated alkenes has been developed<italic>via</italic>1,4(5)-(hetero)aryl migration triggered by radical alkene azidation, trifluoromethylation, or phosphonylation.
Abstract Combined MEK and CDK4/6 inhibition (MEKi + CDK4i) has shown promising clinical outcomes in patients with NRAS-mutant melanoma. Here, we interrogated longitudinal biopsies from a patient who initially responded to MEKi CDK4i therapy but subsequently developed resistance. Whole-exome sequencing functional validation identified an acquired PIK3CAE545K mutation as conferring drug We demonstrate that preexisted rare subpopulation was missed by both research testing, revealed upon...
The fixed dynamic range of traditional biosensors limits their utility in several real applications. For example, viral load monitoring requires the spans orders magnitude; whereas, drugs extremely narrow range. To overcome this limitation, here, we devised tunable biosensing interface using allosteric DNA tetrahedral bioprobes to tune biosensors. Our strategy takes advantage readily and flexible structure design predictable geometric reconfiguration nanotechnology. We reconfigured by...
Abstract Dynamic interactions of enzymes, including programmable configuration and cycling play important roles in the regulation cellular metabolism. Here, we constructed a super DNA‐enzymes molecule (SDEM) that comprises at least two cascade enzymes multiple linked DNA strands to control detect We found SDEM, which glucose oxidase (GOx) horseradish peroxidase (HRP), has 20‐fold lower detection limit 1.6‐fold higher reaction rate than free enzymes. An SDEM can be assembled disassembled...