A5100687183- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Genetic factors in colorectal cancer
- Genomics and Rare Diseases
- Evolution and Genetic Dynamics
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Lung Cancer Treatments and Mutations
- Cancer-related gene regulation
- Genomics and Phylogenetic Studies
- Cancer-related Molecular Pathways
- interferon and immune responses
- Cancer Research and Treatments
- Chromosomal and Genetic Variations
- MicroRNA in disease regulation
- Protease and Inhibitor Mechanisms
- Circular RNAs in diseases
- Cancer, Hypoxia, and Metabolism
- Congenital limb and hand anomalies
- Ginseng Biological Effects and Applications
- Entomopathogenic Microorganisms in Pest Control
- Protein Degradation and Inhibitors
- Plant responses to water stress
Shenyang Pharmaceutical University
2025
Ontario Institute for Cancer Research
2012-2023
Shanghai Jiao Tong University
2023
Shanghai Ninth People's Hospital
2023
The People's Hospital of Guangxi Zhuang Autonomous Region
2013-2022
Linyi University
2022
Inner Mongolia Agricultural University
2021
Institute of Grassland Research
2021
Chinese Academy of Agricultural Sciences
2021
Guangzhou University of Chinese Medicine
2021
Abstract Background High-throughput sequencing allows the detection and quantification of frequencies somatic single nucleotide variants (SNV) in heterogeneous tumor cell populations. In some cases, evolutionary history population frequency subclonal lineages cells present sample can be reconstructed from these SNV measurements. But automated methods to do this reconstruction are not available conditions under which is possible have been described. Results We describe uniquely or multiple...
Abstract In cancer, the primary tumour’s organ of origin and histopathology are strongest determinants its clinical behaviour, but in 3% cases a patient presents with metastatic tumour no obvious primary. Here, as part ICGC/TCGA Pan-Cancer Analysis Whole Genomes (PCAWG) Consortium , we train deep learning classifier to predict cancer type based on patterns somatic passenger mutations detected whole genome sequencing (WGS) 2606 tumours representing 24 common types produced by PCAWG...
Abstract Sex differences have been observed in multiple facets of cancer epidemiology, treatment and biology, most cancers outside the sex organs. Efforts to link these clinical specific molecular features focused on somatic mutations within coding regions genome. Here we report a pan-cancer analysis whole genomes 1983 tumours 28 subtypes as part ICGC/TCGA Pan-Cancer Analysis Whole Genomes (PCAWG) Consortium. We both confirm results exome studies, also uncover previously undescribed...
The successes of targeted drugs with companion predictive biomarkers and the technological advances in gene sequencing have generated enthusiasm for evaluating personalized cancer medicine strategies using genomic profiling. We assessed feasibility incorporating real-time analysis somatic mutations within exons 19 genes into patient management. Blood, tumor biopsy archived samples were collected from 50 patients recruited four centers. Samples analyzed three technologies: exon Pacific...
Long non-coding RNAs (lncRNAs) refer to longer than 200 nucleotides in length.Numerous studies have confirmed that lncRNAs are involved almost all biological processes [7].As for tumors, play an important regulatory role tumor proliferation, survival, metastasis, and so on [8][9][10][11].Recently, the Genetics Epigenetics Research Laboratory of University Texas has demonstrated closely regulating various human cancers [12].The "star molecules" such as MALAT1, HOTAIR, H19 function oncogenes...
In this study, to identify an appropriate macroporous resin for the purification of crude extract derived from Simiao Yong’an Decoction (SMYAD), five distinct resins were evaluated based on their adsorption and desorption performance. Through comparative analysis, D101 was determined be most suitable candidate. Investigations into mechanism revealed that process followed pseudo-second-order kinetics well described by Langmuir isotherm model. Thermodynamic analysis further indicated...
In response to arterial injury, medial vascular smooth muscle cells (VSMCs) proliferate and migrate into the intima, contributing development of occlusive disease. The LIM protein cysteine-rich (CRP) 2 associates with actin cytoskeleton may maintain cytoarchitecture. CRP2 also interacts transcription factors in nucleus mediate SMC gene expression. To test hypothesis that be an important regulator or function we generated Csrp2 (gene symbol mouse gene)-deficient (Csrp2(-/-)) mice by targeted...
Abstract Background Recommendations for perioperative therapy in head and neck cancer are not explicit recurrence occurs frequently. Circulating tumor DNA is an emerging biomarker, but has been extensively explored detection of cancer. Methods Patients diagnosed with squamous cell carcinoma were recruited into the study protocol. Tumors sequenced to identify patient‐specific mutations. Mutations then identified plasma circulating from pre‐treatment blood samples longitudinally during...
Berberine, a quaternary amine and isoquinoline alkaloid, has been proposed to have antimetastatic effects on many types of tumor cells; however, its exact inhibitory mechanisms with bladder cancer cells remain unclear. We used berberine siRNA technology interfere the expression heparanase in T24 cells, detected mRNA protein by reverse transcription PCR Western blot respectively, investigated their migration invasion using transwell chamber. Our results showed that both were highly expressed...
ABSTRACT Chromatin structure has a major influence on the cell-specific density of somatic mutations along cancer genome. Here, we present pan-cancer study in which searched for putative cell-of-origin 2,550 whole genomes, representing 32 types by matching their mutational landscape to regional patterns chromatin modifications ascertained 104 normal tissue types. We found that, almost all types, can be predicted solely from DNA sequences. Our analysis validated hypothesis that high-grade...
Drought is a major limiting factor in foraging grass yield and quality. Medicago ruthenica ( M. ) high-quality forage legume with drought resistance, cold tolerance, strong adaptability. In this study, we integrated transcriptome, small RNA, degradome sequencing identifying response genes, microRNAs (miRNAs), key miRNA-target pairs under rewatering treatment conditions. A total of 3,905 genes 50 miRNAs (45 conserved 5 novel miRNAs) were significantly differentially expressed three test...
Our previous studies have elucidated a possible connection between long intergenic non-protein coding RNA 2570 (LINC02570) and nasopharyngeal carcinoma (NPC). However, the precise mechanism by which LINC02570 promotes NPC remains unknown. We used quantitative polymerase chain reaction (qPCR) to detect expression in cell lines, tissues, chronic rhinitis tissues. Subcellular localization was confirmed fluorescence situ hybridization (FISH). The effects of stable knockdown overexpression on...
Major histocompatibility complex class I-related chains A (MICA), a ligand of Natural killer group 2, member D (NKG2D) receptor, is broadly upregulated in epithelial originated tumor cells. MICA plays critical role the immune surveillance against cells and associated with prognosis several malignancies. The aim this study to evaluate clinical biological significance clear cell renal carcinoma (ccRCC). expression was analyzed by quantitative real-time PCR (qRT-PCR) immunohistochemistry (IHC)....
MicroRNAs (miRNAs) are key regulators during tumor initiation and progression. MicroRNA-375 (MiR-375) has been proven to play a tumor-suppressive role in various types of human malignancies; however, its biological clear cell renal carcinoma (ccRCC) remains unclear. The purpose this study was explore the biologic as well underlying mechanism miR-375 ccRCC progression.Quantitative polymerase chain reaction (qPCR) applied test expression tissues lines by t-test. Functional experiments were...
Using sequencing information to guide clinical decision-making requires coordination of a diverse set people and activities. In genomics, the process typically includes sample acquisition, template preparation, genome data generation, analysis identify confirm variant alleles, interpretation significance, reporting clinicians. We describe software application developed within genomics study, support this entire process. The tracks patients, samples, genomic results, decisions reports across...
To study the differentiated expression of proto-oncogene Pokemon in nasopharyngeal carcinoma (NPC) cell lines and tissues, mRNA protein levels CNE1, CNE2, CNE3 C666-1 were detected separately by reverse transcription polymerase chain reaction (RT-PCR), real-time PCR Western-blotting. The immortalized epithelial line NP69 was used as a control. level biopsy specimens from chronic rhinitis patients undifferentiated non keratinizing NPC determined Western-blotting arranged high to low:...
Oxidative stress can cause the excessive generation of reactive oxygen species (ROS) and has various adverse effects on muscular mitochondria. Qiangji Jianli decoction (QJJLD) is an effective traditional Chinese medicine (TCM) that widely applied to improve muscle weakness, it active constituents prevent mitochondrial dysfunction. To investigate protective mechanism QJJLD against hydrogen peroxide‐ (H 2 O ‐) mediated dysfunction in L6 myoblasts. Cell viability was determined with MTT assay....
Cysteine-rich protein (CRP)2 is a member of the LIM-only CRP family that expressed in vascular smooth muscle cells (VSMC). To gain insight into transcription CSRP2 (gene name for CRP2) VSMC, we analyzed 5′-flanking sequence gene. We showed previously 4,855 bp gene directed lacZ reporter expression, primarily VSMC transgenic mice. further define regulatory sequences important expression series promoter constructs containing deletions upstream nuclear-localized were generated and analyzed....
Although high expression of ZBTB7A is positively relative to metastasis in nasopharyngeal carcinoma (NPC) patients, the association between its low and NPC remains unclear. The present study aimed definitely identify association.The level was effectively knocked down by stable transfection short hair RNA plasmid cell lines CNE2 5-8F (shRNA-CNE2 shRNA-5-8F), compared with cells that stably transfected empty (NC-CNE2 NC-5-8F). levels were assessed real-time polymerase chain reaction Western...
The present study aimed to determine the association between changes in Zbtb7a expression levels and heterogeneity of nasopharyngeal carcinoma (NPC) CNE3 sublines. sublines were established by screening serial dilution continuous passage. Proliferative ability tumorigenicity analyzed separately soft-agar colony formation mouse studies. NPC tissues from mice histological evaluation immunohistochemistry. mRNA protein quantitative reverse transcription polymerase chain reaction western...