Cell death plays an important role in the regulation of inflammation and may be result inflammation. The maintenance tissue homeostasis necessitates both recognition removal invading microbial pathogens as well clearance dying cells. In past few decades, emerging knowledge on cell has enriched our molecular understanding signaling pathways that mediate various programs multiple types inflammatory responses. This review provides overview major related to Modification is likely a logical...

Hemorrhagic shock (HS) often renders patients more susceptible to lung injury by priming for an exaggerated response a second infectious stimulus. Acute (ALI) is major component of multiple organ dysfunction syndrome following HS and regularly serves as cause patient mortality. The vascular endothelium active that has central role in the development ALI through synthesizing releasing number inflammatory mediators. Cell pyroptosis caspase-1-dependent regulated cell death, which features rapid...

Endothelial cells' (EC) injury is a major step for the pathological progression of atherosclerosis. Recent study demonstrated that thymic stromal lymphopoietin (TSLP) exerts protective role in However, effect TSLP and exact molecular mechanism involved EC remains unknown. In present study, we found long noncoding RNA (lncRNA) HOTAIR was much lower from atherosclerotic plaque. Functional assays showed facilitated cell proliferation migration, suppressed apoptosis EC. Moreover, functions...

It has been reported that electromagnetic fields (EMFs) can promote the healing of non-union, osteogenesis and differentiation osteoblasts. However, its mechanism not unravelled. In this study, we detected some response induced by EMF evaluated importance these signals for EMF-induced in bone marrow mesenchymal stem cells (MSCs). We characterized expression markers MSCs, using RT–PCR real-time PCR. Western blot was used to detect signalling pathways. found could along with several osteogenic...

An imbalance in the osteogenesis and adipogenesis of bone marrow-derived stromal cells (BMSCs) is a crucial pathological factor development osteoporosis. Growing evidence suggests that extracellular nucleotide signaling involving P2 receptors plays significant role metabolism. The aim present study was to investigate effects uridine triphosphate (UTP) on osteogenic adipogenic differentiation BMSCs, elucidate underlying mechanisms. BMSCs determined by measuring mRNA protein expression levels...

To investigate the expression of targeting protein for Xenopus kinesin-like 2 (TPX2) in breast cancer tissue and to explore its role proliferation, migration invasion cells.The mRNA expressions TPX2 cell lines were assessed by quantitative RT-PCR Western blot. The effect with RNA interference on cells was observed MTT Transwell assays.Both upregulated tissues compared tumor-adjacent tissue. also lines, interfered small interfering could inhibit inhibiting matrix metalloproteinase-2...

Abstract The mating system transition in polyploid Brassica napus (AACC) from out-crossing to selfing is a typical trait differentiate it their diploid progenitors. Elucidating the mechanism of has profound consequences for understanding speciation and evolution B. . Functional complementation experiment shown that insertion 3.6 kb into promoter self-incompatibility male determining gene, BnSP11-1 leads its loss function inserted fragment was found be non-autonomous Helitron transposon....

Self-incompatibility (SI) is a widespread mechanism in angiosperms that prevents inbreeding by rejecting self-pollen. However, the regulation of SI response Brassica napus not well understood. Here, we report M-locus protein kinase (MLPK) BnaMLPKs, functional homolog BrMLPKs rapa, controls B. napus. We identified four paralogue MLPK genes napus, including BnaA3.MLPK, BnaC3.MLPK, BnaA4.MLPK, and BnaC4.MLPK. Two transcripts BnaA3.MLPKf1 BnaA3.MLPKf2, were generated alternative splicing. Tissue...

Oral squamous cell carcinoma (OSCC) is the most frequent oral malignancy. Recent studies have revealed that long non-coding RNA (lncRNA) PVT1 plays important roles in pathogenesis of various cancers. However, functional OSCC progression and cisplatin resistance not been elucidated.In this study, expression level cisplatin-sensitive cisplatin-resistant tissues lines was determined using qRT-PCR. Gain-of-function loss-of-function assays were performed to explore biological proliferation...

Abstract Electromagnetic field (EMF) stimulation is clinically beneficial for fracture nonunion and a wide range of bone disorders. However, no consensus has been reached on the optimal parameters EMF. The exact mechanism by which EMFs enhance osteogenesis also not defined. In present study, sinusoidal 1 mT EMF at frequencies 10, 30, 50, 70 Hz were administered to rat marrow mesenchymal stromal cells (rBMSCs) in cyclic mode 2 h exposures followed 4 culture without exposure. cell viability,...

Cardiac hypertrophy is characterized by maladaptive tissue remodeling that may lead to heart failure or sudden death. MicroRNAs (miRs) are negative regulators of angiotensin II and the receptor subtype 1 (AGTR1), which two components involved in cardiac hypertrophy. In present study, interaction between (AGTR1) signaling miR-155 was investigated. Rat H9C2 (2-1) cardiomyocytes were transfected with analogues inhibitors, then stimulated induce expression revealed be altered following...

Abstract Background Osteosarcoma is the most common malignant tumor in bone and its prognosis has reached a plateau past few decades. Recently, metabolic reprogramming attracted increasing attention field of cancer research. In our previous study, P2RX7 been identified as an oncogene osteosarcoma. However, whether how promotes osteosarcoma growth metastasis through remains unexplored. Methods We used CRISPR/Cas9 genome editing technology to establish knockout cell lines. Transcriptomics...

The arachidonic acid-cytochrome P450 2J2-epoxyeicosatrienoic acid (AA-CYP2J2-EET) metabolic pathway has been identified to be protective in the cardiovascular system. This study explored effects of AA-CYP2J2-EET on cardiac fibrosis from perspective fibroblasts and underlying mechanisms. In vivo studies, 8-week-old male CYP2J2 transgenic mice (aMHC-CYP2J2-Tr) littermates were infused with angiotensin II (Ang II) or saline for 2 weeks. Results showed that overexpression increased EET...

Gastric cancer is one of the most lethal malignancies worldwide. The aberrant expression microRNA-31 (miR-31) has been reported in gastric cancer; however, its regulation mechanisms are still unclear. Here, we confirmed that miR-31 was significantly decreased tissue and cell lines. Ectopic potentially suppresses proliferation induced early apoptosis cells. Furthermore, regulated as a result epigenetic mechanisms. downregulation associated with promoter DNA methylation status Moreover, found...

Our previous studies have elucidated a possible connection between long intergenic non-protein coding RNA 2570 (LINC02570) and nasopharyngeal carcinoma (NPC). However, the precise mechanism by which LINC02570 promotes NPC remains unknown. We used quantitative polymerase chain reaction (qPCR) to detect expression in cell lines, tissues, chronic rhinitis tissues. Subcellular localization was confirmed fluorescence situ hybridization (FISH). The effects of stable knockdown overexpression on...

Abstract Background Osteosarcoma is the most common primary malignant bone tumor in adolescents and children prone to develop lung metastasis. Its prognosis has been virtually unimproved over last few decades, especially patients with metastases, who suffer from a dismal survival. Recently, increasing attention devoted monocarboxylate transporters-related (MCTs) metabolic reprogramming. However, role of MCT1 MCT4 osteosarcoma progression underlying mechanisms remain be further elucidated....

Brassica species exhibit both compatible and incompatible pollen-stigma interactions, however, the underlying molecular mechanisms remain largely unknown. Here, RNA-seq technology was applied in a comprehensive time-course experiment (2, 5, 10, 20 30 min) to explore gene expression during compatible/incompatible interactions stigma. Moderate changes of were observed pollination (PC) (PI) within 10 minutes, whereas drastic showed up by especially PI. Stage specific DEGs (Differentially...

ARHI is an imprinted tumor suppressor gene and its methylation suppresses transcription levels to cause the development progression of malignant tumors. Zebularine exerts a demethylation function for genes. Our study aims investigate effect mechanism action zebularine on epigenetic modification gene, whether this may modulate viability apoptosis human osteosarcoma cells. We found that inhibited promoted in potentiated expression at both protein mRNA level. This was related downregulation...

Primary colon adenocarcinoma is responsible for high rates of mortality worldwide. The late diagnosis and lack reliable biomarkers therapeutic targets forms a bottleneck in the treatment cancer. In present study, potential an important microRNA (miR), namely miR‑145, was investigated primary cells. results revealed that expression miR‑145 significantly (P<0.05) downregulated cells ectopic cancer inhibited proliferation by promoting apoptosis SW480 Furthermore, bioinformatics analysis exerts...

Background and Purpose The calcium antagonist amlodipine exerts important cardioprotective effects by modulating smooth muscle endothelial functions. However, the mechanisms underlying these are incompletely understood. Experimental Approach Western blotting was used to compare expression of key genes involved in vascular cell (VSMC) phenotype conversion. Recombinant adeno‐associated virus system regulate miRNA rats via tail vein. Bioinformatics predict transcriptional regulation miR‐21...

Abstract Background In recent years, long non-coding RNAs (lncRNAs) have attracted much attention because of its regulatory role in occurrence and progression tumors, including triple-negative breast cancer (TNBC). LncRNA PITPNA antisense RNA 1 (PITPNA-AS1) has been explored some cancers, whereas function molecular mechanism TNBC remain unclear. Methods PITPNA-AS1 expression tissues cells was determined by RT-qPCR. cell viability, proliferation, migration, invasion were assessed with CCK-8,...

Self-incompatibility (SI) in plants genetically prevents self-fertilization to promote outcrossing and genetic diversity. Its hybrids Brassica have been widely cultivated due the propagation of SI lines by spraying a salt solution. We demonstrated that suppression napus from edible solution treatment was ascribed sodium chloride independent S haplotypes, but it did not obviously change expression SI-related genes. Using isobaric tags for relative absolute quantitation (iTRAQ) technique, we...