A5011807009- Pharmacogenetics and Drug Metabolism
- Pharmacological Effects and Toxicity Studies
- Pharmaceutical studies and practices
- Pharmaceutical Practices and Patient Outcomes
- Drug Transport and Resistance Mechanisms
- Atrial Fibrillation Management and Outcomes
- Pharmacovigilance and Adverse Drug Reactions
- Anesthesia and Sedative Agents
- Biosimilars and Bioanalytical Methods
- Antibiotics Pharmacokinetics and Efficacy
- Pain Management and Opioid Use
- Cardiac electrophysiology and arrhythmias
- Inflammatory mediators and NSAID effects
- Antiplatelet Therapy and Cardiovascular Diseases
- Cancer Treatment and Pharmacology
- Renal Transplantation Outcomes and Treatments
- Drug-Induced Hepatotoxicity and Protection
- Patient Safety and Medication Errors
- Computational Drug Discovery Methods
- Anesthesia and Pain Management
- HIV/AIDS drug development and treatment
- HIV-related health complications and treatments
- Health, Medicine and Society
- COVID-19 Clinical Research Studies
- Pharmacology and Obesity Treatment
University Hospital of Geneva
2016-2025
University of Geneva
2015-2025
Geneva College
2013-2025
Swiss Centre for Applied Human Toxicology
2012-2024
HES-SO University of Applied Sciences and Arts Western Switzerland
2021
Hôpital Beau-Séjour
2004-2019
Ibero American University
2015
GTx (United States)
2012
Opioids are mainly used to treat both acute and chronic pain. Several opioids metabolized some extent by CYP2D6 (codeine, tramadol, hydrocodone, oxycodone, methadone). Polymorphisms in have been studied for an association with the clinical effect safety of these drugs. Other genes that their opioid or adverse events include OPRM1 (mu receptor) COMT (catechol‐O‐methyltransferase). This guideline updates expands 2014 Clinical Pharmacogenetics Implementation Consortium (CPIC) genotype codeine...
It is unclear whether warfarin protective or harmful in patients with ESRD and atrial fibrillation. This state of equipoise raises the question alternative anticoagulants may have a therapeutic role. We aimed to determine apixaban pharmacokinetics at steady on hemodialysis. Seven received 2.5 mg twice daily for 8 days. Blood samples were collected before after administration days 1 (nondialysis days). Significant accumulation drug was observed between 2.5-mg dose. The area under...
Abstract Clinical question What is the comparative effectiveness of available therapies for chronic pain associated with temporomandibular disorders (TMD)? Current practice TMD are second most common musculoskeletal disorder after low back pain, affecting 6-9% adults globally. jaw and structures may present headaches, earache, clicking, popping, or crackling sounds in joint, impaired mandibular function. clinical guidelines largely consensus-based provide inconsistent recommendations....
Background and purpose: The major drug‐metabolizing enzymes for the oxidation of oxycodone are CYP2D6 CYP3A. A high interindividual variability in activity these because genetic polymorphisms and/or drug–drug interactions is well established. possible role an active metabolite pharmacodynamics has been questioned importance CYP3A‐mediated effects on pharmacokinetics poorly explored. Experimental approach: We conducted a randomized crossover (five arms) double‐blind placebo‐controlled study...
Abstract Advanced liver fibrosis in nonalcoholic steatohepatitis (NASH) is often accompanied by a reduction hepatic fat to the point of complete loss (burnt-out NASH), but mechanisms behind this phenomenon have not been elucidated. Adiponectin raised cirrhosis any cause and has potent antisteatotic activity. In study we examined 65 patients with advanced biopsy-proven NASH (fibrosis stage 3-4) 54 mild disease 0-1) determine if disappearance steatosis correlated changes serum adiponectin. All...
Background and purpose: There is high interindividual variability in the activity of drug‐metabolizing enzymes catalysing oxidation oxycodone [cytochrome P450 (CYP) 2D6 3A], due to genetic polymorphisms and/or drug–drug interactions. The effects CYP2D6 CYP3A modulation on pharmacokinetics remains poorly explored. Experimental approach: A randomized crossover double‐blind placebo‐controlled study was performed with 10 healthy volunteers genotyped for [six extensive (EM), two deficient (PM/IM)...
The suitability of the capillary dried blood spot (DBS) sampling method was assessed for simultaneous phenotyping cytochrome P450 (CYP) enzymes and P-glycoprotein (P-gp) using a cocktail approach. Ten volunteers received an oral capsule containing low doses probes bupropion (CYP2B6), flurbiprofen (CYP2C9), omeprazole (CYP2C19), dextromethorphan (CYP2D6), midazolam (CYP3A), fexofenadine with coffee/Coke (CYP1A2) on four occasions. They alone (session 1), CYP inhibitors fluvoxamine...
Abstract Clinical question What is the role of medical cannabis or cannabinoids for people living with chronic pain due to cancer non-cancer causes? Current practice Chronic common and distressing associated considerable socioeconomic burden globally. Medical increasingly used manage pain, particularly in jurisdictions that have enacted policies reduce use opioids; however, existing guideline recommendations are inconsistent, remains illegal therapeutic many countries. Recommendation The...
Abstract Clinical question What is the comparative effectiveness and safety of commonly used interventional procedures (such as spinal injections ablation procedures) for chronic axial radicular spine pain that not associated with cancer or inflammatory arthropathy? Current practice Chronic a common, potentially disabling complaint, which clinicians often administer procedures. However, clinical guidelines provide inconsistent recommendations their use. Recommendations For people living (≥3...
Cytochromes P450 (CYP) are subject to important interindividual variability in their activity due genetic and environmental factors some diseases. Limited human data support the idea that inflammation downregulates CYP activities. Our study aimed evaluate impact of orthopedic surgery (acute model) on six CYP. This prospective observational was conducted 30 patients who underwent elective hip at Geneva University Hospitals Switzerland. The phenotyping cocktail containing caffeine, bupropion,...
Coronavirus disease 2019 (COVID‐19), caused by severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection, is a with an underlying inflammatory state. We have previously demonstrated that inflammation modulates cytochromes P450 (CYPs) activity in isoform‐specific manner. therefore hypothesized COVID‐19 might also impact CYP activity, and thus aimed to evaluate the of context SARS‐CoV‐2 infection on six main human CYPs activity. This prospective observational study was conducted...
Introduction Cannabis is the most common recreational drug worldwide and synthetic cannabinoid receptor agonists are currently largest group of new psychoactive substances. The aim this study was to compare clinical features outcomes lone acute cannabis toxicity with agonist in a large series presentations European emergency departments between 2013-2020.
We report a patient who presented non-ST segment elevation myocardial infarction in the context of severe normocytic hypochromic anemia related to gastrointestinal bleeding, three months after switching anticoagulant from vitamin K antagonist acenocoumarol direct oral rivaroxaban. High levels both anti-Xa activity and rivaroxaban plasma concentrations were measured despite withdrawal, suggesting reduced elimination/drug clearance. Estimated half-life was 2 3 times longer than usually...
Background: An LC–MS/MS method has been developed for the simultaneous quantification of P-glycoprotein (P-gp) and cytochrome P450 (CYP) probe substrates their Phase I metabolites in DBS plasma. P-gp (fexofenadine) CYP-specific (caffeine CYP1A2, bupropion CYP2B6, flurbiprofen CYP2C9, omeprazole CYP2C19, dextromethorphan CYP2D6 midazolam CYP3A4) were extracted from (10 µl) using methanol. Analytes separated on a reversed-phase LC column followed by SRM detection within 6 min run time....
Cytochrome P450 (CYP) activity can be assessed using a 'cocktail' phenotyping approach. Recently, we have developed cocktail (Geneva cocktail) which combines the use of low-dose probes with low-invasiveness dried blood spots (DBS) sampling technique and single analytical method for six major CYP isoforms. We previously demonstrated that modulation after pre-treatment inhibitors/inducer could reliably predicted Geneva cocktail. To further validate this cocktail, in study, verified whether...