Background: Although N 6 -methyladenosine (m A) plays a very important role in different biological processes, its function the brain has not been fully explored. Thus, we investigated roles of RNA demethylases Alkbh5/Fto cerebral ischemia-reperfusion injury. Methods: We used rat model and primary neuronal cell culture to study m A cortex ischemic penumbra after Alkbh5-shRNA Lv-Fto ( vitro) regulate expression their regulation Results: found that levels increased consecutive increase Alkbh5...

Cerebral ischemic stroke (IS) is still a difficult problem to be solved; energy metabolism failure one of the main factors causing mitochondrion dysfunction and oxidation stress damage within pathogenesis cerebral ischemia, which produces considerable reactive oxygen species (ROS) opens blood-brain barrier. Dichloroacetic acid (DCA) can inhibit pyruvate dehydrogenase kinase (PDK). Moreover, DCA has been indicated with capability increasing mitochondrial uptake promoting glucose in course...

Previous studies have demonstrated that cardiomyocyte apoptosis, ferroptosis, and inflammation participate in the progress of sepsis-induced cardiomyopathy (SIC). Although Islet cell autoantigen 69 (ICA69) is an imperative molecule could regulate immune response numerous illnesses, its function cardiovascular disease, particularly SIC, still elusive. We confirmed LPS significantly enhanced expression ICA69 wild-type (WT) mice, macrophages, cardiomyocytes. The knockout...

Abstract C‐Jun N‐terminal kinase (JNK) is a pivotal MAPK (mitogen‐activated protein kinase), which activated by ischemia brain injury and plays fairly crucial function in cerebral ischemic injury. Emerging studies demonstrated that JNK‐IN‐8 (a JNK inhibitor with high specificity) regulates traumatic through controlling neuronal apoptosis inflammation. However, the of stroke mechanisms underlying about neuroprotection are not well understood. In this work, male rats were treated after...

Neuroinflammation mediated by microglial pyroptosis is an important pathogenic mechanism of septic encephalopathy (SAE). It has been reported that TRIM45 associated with tumours and inflammatory diseases. However, the role in SAE relationship between are unknown. In this study, we found played regulating molecular mechanism.SAE was induced intraperitoneal injection LPS WT AAV-shTRIM45 mice. BV2 cells were treated LPS/ATP vitro. Cognitive function assessed Morris water maze. Nissl staining...

This study aimed to investigate the relationship between unique weather change and acute ischemic stroke (AIS) in southern Zhejiang Province of China provide evidence for better predicting preventing stroke. We retrospectively collected 14,996 patients data from January 2019 December 2021 China. The correlation risk meteorological factors number AIS daily cases were calculated. Wilcoxon rank sum test was used calculate difference typhoon-affected non-affected periods. A prediction model...

Purpose: This study compares the analgesic effects of Thoracoscopic Direct-view Thoracic Paravertebral Nerve Block (DTPVB) with those Ultrasound-guided (UTPVB), providing a clinical reference. Patients and Methods: Sixty-eight patients undergoing three-port video-assisted thoracic surgery (VATS) general anesthesia were randomly assigned to either DTPVB group (Group D, n = 34) or UTPVB U, 34). Both groups received 10 mL injection 0.75% ropivacaine at T4 T7 interspaces. Primary outcomes...

ABSTRACT Sepsis-induced cardiomyopathy (SIC) is one of the most common complications infection-induced sepsis. An imbalance in inflammatory mediators main factor leading to SIC . N 6 -methyladenosine (m A) closely related occurrence and development reader YTH domain containing 1 (YTHDC1) an m A recognition protein. However, role YTHDC1 remains unclear. Herein, we demonstrated that YTHDC1-shRNA inhibits inflammation, reduces mediators, improves cardiac function a LPS-induced mouse model....

To evaluate whether ferroptosis is involved in hyperoxic acute lung injury (HALI) and its mechanisms through the HALI model.HE staining was used to assess pathology after establishment of neonatal rat model. ELISA detect ROS, GPX4, GSH expression. Prussian blue Western Blot were iron deposition expression ferroptosis-related proteins, respectively.The group showed pathological changes with larger fewer alveoli thicker alveolar septa HE staining. detected significant tissue group. GSH, GSS,...

The treatment options for sepsis-associated encephalopathy caused by systemic inflammation are still not sufficient. Protein kinase C interaction protein 1 (PICK1) has attracted much attention because of its important physiological functions in many tissues. However, role remains elusive. Our study results revealed that the expression levels PICK1 mice with lipopolysaccharide-induced were significantly changed, but deficiency led to excessive activation microglia and Toll-like receptor...

Electroacupuncture (EA) is commonly used to treat cerebrovascular diseases. This study aimed clarify the mechanisms of action treatments cerebral ischemic stroke from perspective gut microecology. We a mouse model and cell cultures investigate effects EA on intestinal microflora in mice models middle artery occlusion (MCAO) underlying antioxidant activities metabolites. Fecal microbiota transplantation (FMT) was validate roles microbiota. Metabolomic analysis performed characterize metabolic...

Sepsis is a systemic inflammatory reaction caused by infection. Multiple organ failure ultimately leads to high morbidity and mortality. Unfortunately, therapies against these responses have been unsuccessful due the insufficient underlying pathophysiological evidence. Protein interacting with C-kinase 1 (PICK1) has received considerable attention because of its important physiological functions in many tissues. However, role sepsis-induced acute lung injury (ALI) unclear. In this study, we...

Ca2+/calmodulin-dependent protein kinase II (CaMKII) is a multifunctional serine/threonine that ubiquitously distributed in the central and peripheral nervous systems. Moreover, its phosphorylated (P-CaMKII) involved memory, mood, pain regulation anterior cingulate cortex (ACC). Electroacupuncture (EA) traditional Chinese therapeutic technique can effectively treat chronic inflammatory pain. However, CaMKII-GluA1 role EA analgesia ACC remains unclear. This study investigated of P-CaMKII...

Postoperative nausea and vomiting (PONV) is one of the most common complications after general anesthesia. The traditional comprehensive management PONV usually uses or two drugs, but this regimen fails to meet requirements latest version guidelines. purpose study was evaluate effect transcutaneous electrical acupoint stimulation (TEAS) on high-risk patients who are undergoing laparoscopic gynecological surgery.In total, 162 were randomly divided into an experimental group (n = 81) a control...

Glycogen synthase kinase-3β (GSK3β), fat mass and obesity-associated protein (FTO), toll-like receptors 4 (TLR4) take on critical significance in different biological processes, whereas their interactions remain unclear. The objective was the investigation of interaction effect cerebral ischemia-reperfusion (I/R) injury.The function cortex mouse middle artery occlusion (MCAO) model (each group n = 6) P12 cells oxygen-glucose deprivation/reoxygenation (OGD/R) analyzed using short hairpin...

Objective . Our present study tested whether flurbiprofen axetil could reduce perioperative sufentanil consumption and provide postoperative analgesia with decrease in emergency agitation systemic proinflammatory cytokines release. Methods Ninety patients undergoing tangential excision surgery were randomly assigned to three groups: (1) preoperative dose of 100 mg a 2 μ g/kg 10 mL placebo by patient‐controlled (PCA) pump, (2) PCA (3) pump. Results Preoperative administration decreased...

Electroacupuncture (EA) is widely used in clinical settings to reduce inflammatory pain. Islet-cell autoantigen 69 (ICA69) has been reported regulate long-lasting hyperalgesia mice. ICA69 knockout led reduced protein interacting with C-kinase 1 (PICK1) expression and increased glutamate receptor subunit 2 (GluR2) phosphorylation at Ser880 spinal dorsal horn. In this study, we evaluated the role of antihyperalgesic effects EA underlying mechanism through regulation GluR2 PICK1 Hyperalgesia...

A previous study has demonstrated that pretreatment with electroacupuncture (EA) induces rapid tolerance to focal cerebral ischemia. In the present study, we investigated whether adenosine receptor 1 (A1 R) is involved in EA pretreatment-induced cognitive impairment after ischemia rats. Two hours pretreatment, was induced by middle artery occlusion for 120 min male Sprague-Dawley The neurobehavioral score, function [as determined Morris water maze (MWM) test], neuronal number, and Bax/Bcl-2...

Reperfusion after tourniquet use can induce inflammation and cause remote organ injury. We evaluated the therapeutic effect of transcutaneous electrical acupoint stimulation (TEAS) on inflammatory mediators lung function in patients receiving lower limb tourniquets. Forty undergoing unilateral extremity surgery with were randomly assigned to two groups: TEAS group ischemia-reperfusion (I/R) group. The C-C motif chemokine ligand 2 (CCL2), C-X-C 8 (CXCL8), interleukin-1 (IL-1), interleukin-6...