A5083037852- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Trauma and Emergency Care Studies
- Medical History and Research
- Cardiac Arrest and Resuscitation
- Advanced Biosensing Techniques and Applications
- Chronic Myeloid Leukemia Treatments
- Cancer Immunotherapy and Biomarkers
- Sarcoma Diagnosis and Treatment
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Monoclonal and Polyclonal Antibodies Research
- Molecular Biology Techniques and Applications
- Peptidase Inhibition and Analysis
- Oral and Maxillofacial Pathology
- Chronic Lymphocytic Leukemia Research
- Epigenetics and DNA Methylation
- HER2/EGFR in Cancer Research
- Virus-based gene therapy research
- Mast cells and histamine
- Eosinophilic Disorders and Syndromes
- interferon and immune responses
- Bone Tumor Diagnosis and Treatments
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- Abdominal Trauma and Injuries
Ludwig-Maximilians-Universität München
2023-2025
RWTH Aachen University
2015-2024
Universitätsklinikum Aachen
2015-2024
LMU Klinikum
2024
Novem (Netherlands)
2019-2021
Committee on Publication Ethics
2021
FH Aachen
2007-2020
Unfallkrankenhaus Salzburg
2019
Maastricht University
2017
National Institutes of Health
2004-2015
Abstract We have previously shown that topotecan, a topoisomerase I poison, inhibits hypoxia-inducible factor (HIF)-1α protein accumulation by DNA damage-independent mechanism. Here, we report daily administration of topotecan HIF-1α expression in U251-HRE glioblastoma xenografts. Concomitant with inhibition, caused significant tumor growth inhibition associated marked decrease angiogenesis and HIF-1 target genes tissue. These results provide compelling rationale for testing clinical trials...
Formalin-fixed, paraffin-embedded (FFPE) tissue is the most common specimen available for molecular assays on after diagnostic histopathological examination. RNA from FFPE suffers strand breakage and cross-linking. Despite excellent extraction methods, quality material remains variable. To address factors within tissues, we studied quality, isolating individual elements of fixation processing including length in formalin type buffer incorporated fixative. We examined impact cycle as well....
Multiorgan tropism of SARS-CoV-2 has previously been shown for several major organs. We have comprehensively analyzed 25 different formalin-fixed paraffin-embedded (FFPE) tissues/organs from autopsies fatal COVID-19 cases (n = 8), using histopathological assessment, detection RNA polymerase chain reaction and in situ hybridization, viral protein immunohistochemistry, virus particles transmission electron microscopy. was mainly localized epithelial cells across all Next to lung, trachea,...
Abstract Human tumor xenografts have been used extensively for rapid screening of the efficacy anticancer drugs past 35 years. The selection appropriate xenograft models drug testing has largely empirical and not incorporated a similarity to type origin at molecular level. This study is first comprehensive analysis transcriptome large set pediatric xenografts, which are currently preclinical testing. Suitable representing were identified. It was found that characteristic expression patterns...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is characterized by an abundant desmoplastic stroma. Interactions between cancer and stromal cells play a critical role in tumour invasion, metastasis chemoresistance. Therefore, we hypothesized that gene expression profile of the components pancreatic carcinoma different from chronic pancreatitis reflects interaction with tumour. We investigated eleven tissues PDAC, nine cell lines origin using Affymetrix U133 GeneChip set. The tissue samples...
NKG2D (natural killer group 2, member D) is thought to play an important role in mediating the activation of anticancer immune response. Expression ligands (NKG2DLs) pronounced malignancies and heterogeneity NKG2DL expression remains unclear. Here, we investigate clinical significance NKG2DLs cervical cancer. Immunohistochemical analyses MICA/B, ULBP1, ULBP2, ULBP3, RAET1E, RAET1G were performed using tissue microarray analysis 200 cancers, 327 high-grade intraepithelial neoplasias (CINs),...
Formalin-fixed, paraffin-embedded (FFPE) tissue is the most common specimen available for application of diagnostic assays on after microscopic examination. Not only there a substantial archive available, but FFPE remains best method preparation examination in routine clinical environment. Molecular assays, especially reverse transcription and polymerase chain reaction expression array-based offer significant potential as diagnostic, prognostic, predictive tools, require high quality RNA....
Regulation of chromatin is an important aspect controlling promoter activity and gene expression. Posttranslational modifications core histones allow proteins associated with transcription to access chromatin. Closely promoters actively transcribed genes, trimethylation histone H3 at lysine 4 (H3K4me3) a mark set by several protein complexes. Some these complexes contain the trithorax ASH2 combined MLL oncoproteins. We identified human in complex oncoprotein MYC. This finding, together...
The early application of fibrinogen could potentially reverse haemodilution-induced coagulopathy, although the impact varying concentrations to dilutional coagulopathy has not been studied in vivo. We postulated that concentration is correlated with blood loss a pig model blunt liver injury.Coagulopathy was induced 18 anaesthetized pigs (32 +/- 1.6 kg body weight) by replacing 80% volume hydroxyethylstarch 130/0.4 and Ringer's lactated solution, re-transfusion erythrocytes. Animals were...
The peripheral airway innervation of the lower respiratory tract mammals is not completely functionally characterized. Recently, we have shown in rats that precision-cut lung slices (PCLS) respond to electric field stimulation (EFS) and provide a useful model study neural responses distal airways. Since are known exhibit considerable species differences, here examined PCLS prepared from mice, rats, guinea pigs, sheep, marmosets humans. Peripheral neurons were activated either by EFS or...
This study was designed to identify and validate potential new biomarkers for prostate cancer distinguish patients with without biochemical relapse. Prostate tissue samples analyzed by 2D-DIGE (two-dimensional difference in gel electrophoresis) mass spectrometry (MS) revealed downregulation of secernin-1 (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mi>P</mml:mi></mml:mrow></mml:math>< 0.044) cancer, while vinculin showed significant upregulation...
Studies in which mesenchymal stromal cells (MSC) from the placenta are compared with multiple MSC types other sources rare. The chorionic plate of human is mainly composed fetal blood vessels embedded stroma tissue, lined by trophoblastic and organized into villi (CV) structures. We comprehensively characterized collected postnatal (CV-MSC) analyzing their growth proliferation potential, differentiation, immunophenotype, extracellular matrix production, telomere length, aging phenotype,...
Programmed death-ligand 1 (PD-L1) expression on tumor cells (TC) or tumor-infiltrating immune (IC) correlated in several studies with PD-L1/programmed death-1 (PD-1) checkpoint inhibitor efficacy. Since June 2018, a positive PD-L1 status is required for atezolizumab pembrolizumab treatment of patients advanced metastasized urothelial bladder cancer, who are ineligible cisplatin-containing therapy. We examined technical comparability and inter-reader agreement four clinically developed assays...
In the event of trauma, emergency reversal anticoagulation therapy may be required. However, no specific agents are routinely available for direct oral anticoagulants such as dabigatran. The authors investigated four-factor prothrombin complex concentrate (PCC) treating dabigatran-induced in a porcine polytrauma model.Dabigatran etexilate was given orally 3 days and intravenously on day 4 to 32 pigs. Animals were randomized 1:1:1:1 PCC (25, 50, or 100 U/kg) saline. Study medication...
Clinical use of non-vitamin K antagonist oral anticoagulants is increasingly well established. However, specific agents for reversal these drugs are not currently available. It was to objective this study investigate the impact activated prothrombin complex concentrate (aPCC) on anticoagulant effects dabigatran in a randomised, controlled, porcine trauma model. Twenty-one pigs received and intravenous dabigatran, resulting supratherapeutic plasma concentrations. Twelve minutes after injury...
PD-L1 is a surface molecule which expressed on different types of cells, including antigen presenting vascular endothelial cells and other human tissues. Expression also found tumor cells. as the ligand to PD1 receptor CD8+ T inhibits its cytotoxic effect cell. The modern target therapy uses this interaction inhibit PD-1 stimulate necrosis. To compare expression differences, twelve frequent malignant tumors with ten patients per group were selected. Immunohistochemical stains antibodies for...
Immune checkpoint inhibitors (ICI) represent a new therapeutic approach in recurrent and metastatic head neck squamous cell carcinoma (HNSCC). The patient selection for the PD-1/PD-L1 inhibitor therapy is based on degree of PD-L1 expression immunohistochemistry reflected by manually determined scores. However, manual scoring shows variability between different investigators influenced cognitive visual traps could therefore negatively influence treatment decisions. Automated facilitate...
Abstract Mast cells develop from the myeloid lineage and are released bone marrow as immature cells, which then differentiate at destination tissue based on cues local environment. In liver, mast recruited in diseased states to fibrogenic surroundings rich TGF-β1. The aim of this study was investigate TGF-β1 signaling primary permanent identify common unique mechanisms. TGF-β receptor repertoire is similar among with high expression type I II receptors very low III (Betaglycan Endoglin)....