A5044280079- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Muscle Physiology and Disorders
- Urinary Bladder and Prostate Research
- Mechanical Circulatory Support Devices
- Bone Tissue Engineering Materials
- Molecular Biology Techniques and Applications
- Polymer Surface Interaction Studies
- Cancer Genomics and Diagnostics
- Cell Image Analysis Techniques
- Enzyme Production and Characterization
- Blood properties and coagulation
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Periodontal Regeneration and Treatments
- Telomeres, Telomerase, and Senescence
- Glioma Diagnosis and Treatment
- biodegradable polymer synthesis and properties
- Cancer-related Molecular Pathways
- Alzheimer's disease research and treatments
- Gene expression and cancer classification
- Microtubule and mitosis dynamics
- Electrochemical sensors and biosensors
- Genomics and Rare Diseases
- Prostate Cancer Diagnosis and Treatment
Hospital for Sick Children
2025
Samsung Medical Center
2016-2024
Sungkyunkwan University
2014-2024
Samsung (South Korea)
2023-2024
Ministry of Food and Drug Safety
2004-2008
Seoul National University
1993-1997
Korea Institute of Science and Technology
1993
Testing new ways to identify untapped opportunities for glioblastoma therapies remains highly significant. Amplification and overexpression of MDM2 gene is frequent in disrupting the MDM2-p53 interaction a promising strategy treat cancer. RG7112 first-in class inhibitor recently discovered AMG232 most potent known date. Here, we compared effects these two clinical inhibitors six cell lines ten patient-derived stem cells. Targeted sequencing TP53, genes whole transcriptome analysis were...
Adsorption of proteins (fibrinogen, albumin, and gamma globulin) from plasma onto surface-modified PUs (PU-PEO, PU-SO3, PU-PEO-SO3) was evaluated. Adsorbed fibrinogen at steady state decreased in the order PU-SO3 > PU PU-PEO-SO3 PU-PEO, suggesting that sulfonate groups have specific high affinity to fibrinogen. The intermediate adsorption on can be explained by compensatory effect between low protein binding PEO chain group. In addition, showed a very fast due accessibility group...
Abstract The properties of regenerated cartilage using bone marrow‐derived mesenchymal stem cells (MSCs) and poly lactic‐ co ‐glycolic acid (PLGA) scaffold composites pretreated with TGF‐β3 were investigated compared to the non‐TGF‐β3 treated MSCs/PLGA in a rabbit model. We prepared it for 3 weeks prior transplantation. Then, transplanted osteochondral defect knee. After 12 transplantation, 10 rabbits which showed cartilaginous regeneration. In gross morphology, smooth, flush, transparent...
Abstract Targeted capture massively parallel sequencing is increasingly being used in clinical settings, and as costs continue to decline, use of this technology may become routine health care. However, a limited amount tissue has often been challenge meeting quality requirements. To offer practical guideline for the minimum input DNA targeted sequencing, we optimized evaluated performance depending on amount. First, using various amounts DNA, compared commercially available library...
Extracellular matrix (ECM) components play an important role in maintaining skeletal muscle function, but excessive accumulation of ECM interferes with regeneration after injury, eventually inducing fibrosis. Increased oxidative stress level caused by dystrophin deficiency is a key factor fibrosis Duchenne muscular dystrophy (DMD) patients. Mesenchymal stem cells (MSCs) are considered promising therapeutic agent for various diseases involving In particular, the paracrine factors secreted...
The aim of this study was to evaluate the therapeutic effects and mechanisms Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) in an animal model Duchenne muscular dystrophy (DMD). Mdx mice (3–5 months old) were administered five different doses WJ-MSCs through their tail veins. A week after injection, grip strength measurements, creatine kinase (CK) assays, immunohistochemistry, western blots performed for comparison between healthy mice, mdx control WJ-MSC-injected mice. exerted...
Customized gene-panel tests, based on next-generation sequencing, have demonstrated their usefulness in a plethora of clinical settings. As with other diagnostic techniques, sequencing for purposes requires precise quality control (QC) measures to ensure its reliability. Only detected variants are currently recorded reports; however, identifying whether nondetected variant is true or false negative regarded essential setting and, thus, comprehensive QC measure demand. Conventional metrics,...
Mesenchymal stem cells (MSCs) are safe, and they have good therapeutic efficacy through their paracrine action. However, long-term culture to produce sufficient MSCs for clinical use can result in side-effects, such as an inevitable senescence the reduction of MSCs. In order overcome this, primary conditions be modified simulate cells’ niche environment, resulting accelerated proliferation, achievement target production yield at earlier passages, improvement efficacy. We exposed Wharton’s...
Abstract Lumbrokinase is a potent fibrinolytic enzyme purified from the earthworm, Lumbricus rubellus. We immobilized 18 IU/cm 2 of lumbrokinase to polyurethane using maleic anhydride methylvinyl ether copolymer (MAMEC) as an carrier, and proteolytic activities were assayed. Immobilized retained about 34% its activity, compared with soluble activity. showed stability against thermal inactivation degradation within various pH range. The optimal shifted 1.0 unit upward enzyme. Upon exposure...
Purpose This study aimed to set priorities for improving the medical device distribution structure and suggest an innovative improvement plan using analytic hierarchy process (AHP) method, focusing on stakeholders in industry. Methods conducted a survey with 35 specialists AHP which is multiple-criteria decisionmaking methodology, order plans address problems faced by structure. Results The analysis showed that supply stability was most important factor, followed greater transparency,...
Abstract Replicative senescence of mesenchymal stem cells (MSCs) caused by repeated cell culture undermines their potential as a therapy because the reduction in proliferation and therapeutic potential. Glutaminase-1 (GLS1) is reported to be involved survival senescent cells, inhibition GLS1 alleviates age-related dysfunction via removal. In present study, we attempted elucidate association between MSC GLS1. We conducted vitro vivo experiments analyze effect on senolysis effects MSCs....
Abstract Potent and novel fibrinolytic enzymes (lumbrokinase [LK]) were extracted from the earthworm, Lumbricus rubellus . These very stable showed greater antithrombotic activity than other currently used proteins. An LK fraction showing most potent was immobilized onto a polyurethane (PU) surface to investigate its enzymatic activity. A methanol‐extracted PU coated with 3% (wt/vol) maleic anhydride methylvinyl ether copolymer (MAMEC)/tetrahydrofuran (THF) solution, incubated in an...
Aim The current standard diagnostic test to evaluate bladder outlet obstruction (BOO) is pressure-flow study (PFS). penile cuff (PCT) was introduced as a non-invasive alternative PFS determine the isovolumetric pressure and also flow rate. aim of accuracy acceptability PCT, compared those PFS, in assessment BOO men. Methods A total 146 consecutive men with an International Prostate Symptom Score (IPSS) >12 lower urinary tract symptoms (LUTS) for >6 months were included this...
Glioblastoma multiforme (GBM) is recognized as the most common and lethal form of central nervous system cancer. To cure GBM patients, many target-specific chemotherapeutic agents have been developing. However, 2D monolayer cell-based toxicity efficacy tests did not efficiently screen due to pool reflection in vivo microenvironments (cell-to-cell cell-to-extracellular matrix interaction). In this study, we used a 3D cell-based, high-throughput screening method reflecting using micropillar...
A high-throughput clonogenic assay with a micropillar-microwell chip platform is proposed by using the colony area of glioblastoma multiforme (GBM) patient-derived cells (PDCs) from images. Unlike conventional cell lines, PDCs tumor are composed heterogeneous populations, and some populations form colonies during culture while rest die off or remain unchanged, thus causing diverse distribution size. Therefore, area-based analysis total not sufficient to estimate viability toxicity responses....
(1) Objective: To investigate the factors that affect rates of neutralizing antibody production and duration after vaccination using newly developed SARS-CoV-2 POCT. (2) Methods: The immunoglobulin in clinical subjects who completed various vaccines was analyzed POCT, semi-quantitative interpreted by measurement application, quantified titers were ELISA. (3) Results: According to performance analysis sensitivity specificity 96.8% (90/93) 97.7% (167/171), respectively, for S1 RBD IgG...
In tissue engineering and wound-healing applications, dermal substitutes are used to provide fibroblasts with the mechanical support for their growth then facilitate skin formation. this study, three-dimensional porous poly(lactic-co-glycolic acid) (PLGA) 65/35 scaffolds were prepared composites of human fetal fabricated as a tissue-engineered substitute. The function compatibility artificial substitute evaluated at levels gene expression (by RT-PCR) protein (total collagen quantities), well...
Mesenchymal stem cells (MSCs) are effective therapeutic agents that contribute to tissue repair and regeneration by secreting various factors. However, donor-dependent variations in MSC proliferation potentials result variable production yields clinical outcomes, thereby impeding MSC-based therapies. Hence, selection of MSCs with high would be important for application MSCs. This study is aimed at identifying the upregulated genes human Wharton's jelly-derived (WJ-MSCs) potential using mRNA...
Mesenchymal stem cells (MSCs) have emerged as a promising tool for the treatment of Alzheimer’s disease (AD). Previous studies suggested that coculture human MSCs with AD in an vitro model reduced expression amyloid-beta 42 (Aβ42) medium well overexpression amyloid-beta- (Aβ-) degrading enzymes such neprilysin (NEP). We focused on role primed (human Wharton’s jelly-derived mesenchymal (WJ-MSCs) exposed to cell line via system) reducing levels Aβ and inhibiting death. demonstrated mouse...
Fibrinogen adsorptions to surface modified polyurethanes (PU, PU-PEO, and PU-PEO-SO3) were studied from plasma in vitro. PU PU-PEO surfaces demonstrated that initial adsorption increases with increasing concentration kinetic profiles time as a function of concentration, but after the plateau is reached, its amount decreases (0.2-2.0%) (1-120 min) increase, respectively. In contrast, PU-PEO-SO3 showed almost same regardless time, which due high affinity sulfonate group fibrinogen. All...