A5014157846- Long-Term Effects of COVID-19
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- HIV Research and Treatment
- COVID-19 Clinical Research Studies
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Infant Development and Preterm Care
- Vaccine Coverage and Hesitancy
- Virus-based gene therapy research
- Childhood Cancer Survivors' Quality of Life
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Infectious Encephalopathies and Encephalitis
- Space Exploration and Technology
- COVID-19 and Mental Health
- Renal and related cancers
- Viral Infections and Vectors
- RNA Research and Splicing
- Cerebral Palsy and Movement Disorders
- Genetics, Aging, and Longevity in Model Organisms
- Spaceflight effects on biology
- Phytochemistry and Biological Activities
- Infectious Diseases and Tuberculosis
University of Nebraska Medical Center
2020-2024
Children's Hospital & Medical Center
2020-2023
Tohoku Medical and Pharmaceutical University
2005
Icahn School of Medicine at Mount Sinai
2004
Thomas Jefferson University
2000
University of Pennsylvania
1992-1994
The Wistar Institute
1992
California University of Pennsylvania
1992
University of Illinois Chicago
1992
National Institute on Aging
1990
Abstract The accumulation of myeloid suppressor cells (MSCs) is associated with immune suppression in tumor-bearing mice and cancer patients. suppressive activity MSC correlates the expression markers Gr-1, CD115 (macrophage colony-stimulating factor receptor), F4/80. Gr-1+CD115+ MSCs, addition to being able suppress T-cell proliferation vitro, can induce development Foxp3+ T regulatory (Treg) vivo, which are anergic suppressive. Furthermore, secretion interleukin (IL)-10 transforming growth...
Recently, immunization techniques in which DNA constructs are introduced directly into mammalian tissue vivo have been developed. In theory, gene inoculation should result the production of antigenic proteins a natural form immunized host. Here we present use such technique for mice with human immunodeficiency virus type 1 (HIV-1) envelope construct (pM160). Mice were injected intramuscularly pM160 and subsequently analyzed their anti-HIV immune responses. Antisera collected from inoculated...
Most research to understand postacute sequelae of SARS-CoV-2 infection (PASC), or long COVID, has focused on adults, with less known about this complex condition in children. Research is needed characterize pediatric PASC enable studies underlying mechanisms that will guide future treatment.
Abstract In previous reports, systemic administration of a stimulatory monoclonal antibody directed against the 4‐1BB receptor had no effect on survival or tumor burden in mice inoculated with poorly immunogenic B16‐F10 melanoma. We combined IL‐12 gene transfer costimulation to explore previously noted cooperative anti‐tumor this model tumor. hypothesize that innate immune response mediated by IL‐12‐activated natural killer (NK) cells initiates activation system, leading priming T cells,...
Abstract IMPORTANCE Prior studies have demonstrated the effectiveness of COVID-19 vaccines in children and adolescents. However, benefits vaccination these age groups with prior infection remain underexplored. OBJECTIVE To evaluate preventing reinfection various Omicron subvariants (BA.1/2, BA.4/5, XBB, later) among 5- to 17-year-olds SARS-CoV-2 infection. DESIGN A target trial emulation through nested designs distinct study periods. SETTING The utilized data from Research COVID Enhance...
Poor bioavailability, rapid degradation, antigenicity, and high cost often limit the use of proteinaceous pharmaceuticals. One goal structural biochemistry is reduction complex molecules to small functional units that are amenable high-resolution analysis modification. The dissection proteins into synthetic conformationally restricted components an important step in design low molecular weight nonpeptides mimic activity native protein. We have developed a reverse-turn mimetic system explore...
The CD4 protein expressed on helper T lymphocytes is a restriction element for major histocompatibility class II immune responses. This molecule also used by the human immunodeficiency virus as its specific cellular receptor facilitating binding of to cells. As soluble forms inhibit HIV infection in tissue culture, attention has focused this molecule. Bacterially produced would facilitate studies biology However, bacterially must be refolded assumption interaction with conformationally...
Abstract A major cytopathic effect seen upon in vitro infection of CD4+ human T cells by the HIV is cell-to-cell fusion that results giant cell (or syncytium) formation. Membrane required for cellfree virions and syncytium We report here surface molecule, CD7, important HIV-1 process. CD7 a roughly 40-kDa glycoprotein member Ig supergene family expressed early ontogeny thymocytes on majority peripheral blood cells, as well NK small subpopulation B cells. Anti-CD7 mAbs inhibited HIV-1-induced...
The human immunodeficiency virus (HIV)-1 envelope glycoprotein is synthesized as a precursor (gp160) and subsequently cleaved to generate the external gp120 transmembrane gp41 glycoproteins. Both have been demonstrated mediate critical functions of HIV, including viral attachment fusion with cell membrane. antigenic variability HIV-1 has presented significant problem in design appropriate successful vaccines offers one explanation for ability HIV evade immune surveillance. Therefore,...
BACKGROUND Anti-TNF-alpha therapies were the first class of biologics to be used in treatment moderate severe IBD. Immunosuppression status that develops from using anti-TNF-a increases risk serious and opportunistic infections. We present here a rare case infection developed an IBD patient while on anti-TNF therapy. CASE REPORT Our was 14-year-old boy with history chronic sinusitis ulcerative colitis who had been infliximab therapy for last 3 years. He presented facial swelling worsening...
We examined the effect of PGE1 on expression plasminogen activator inhibitor-1 (PAI-1) mRNA induced by tumor necrosis factor-alpha (TNF-alpha) in human mesangial cells, because PAI-1 is one major factors for progression glomerulosclerosis. The was increased after stimulation with TNF-alpha, and it diminished pre-incubation PGE1. Next, we phosphorylation mitogen activated protein kinase (MAPK) family Akt. TNF-alpha p44/42 MAPK, p38 SAPK/JNK Akt cells. inhibited Akt, but not MAPK MAPK....
The polymerase chain reaction (PCR) allows rapid amplification of DNA known sequence. In many situations, part a genetic sequence is known, but adjacent sequences interest are unknown. This common in investigations antigen receptor genes from B and T lymphocytes, which composed constant region variable region, for the may not be known. Herein described method to amplify when information available only one primer. procedure utilizes primer conjunction with mixture short random primers....