A5100431722- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Glycation End Products research
- Transcranial Magnetic Stimulation Studies
- Nigella sativa pharmacological applications
- Neurological Disorders and Treatments
- Epigenetics and DNA Methylation
- Renal and related cancers
- Berberine and alkaloids research
- Renal cell carcinoma treatment
- Neurological disorders and treatments
- Neurological Disease Mechanisms and Treatments
- Ion Channels and Receptors
- Wnt/β-catenin signaling in development and cancer
- Barrier Structure and Function Studies
- MicroRNA in disease regulation
- Galectins and Cancer Biology
- Signaling Pathways in Disease
- Mass Spectrometry Techniques and Applications
- Cell Adhesion Molecules Research
- Neuroscience of respiration and sleep
- Pain Management and Treatment
- Brain Tumor Detection and Classification
- Functional Brain Connectivity Studies
- Pharmacological Effects of Natural Compounds
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Jianghan University
2023-2024
Beckman Research Institute
2023
Hebei Medical University
2023
City of Hope
2023
First Affiliated Hospital of Xi'an Jiaotong University
2012-2023
Institute of Biomedical Science
2023
Daping Hospital
2023
Army Medical University
2017-2023
Harbin Medical University
2022
First Affiliated Hospital of Harbin Medical University
2022
Abstract Background The phosphoinositide 3-kinase (PI3K)/Akt pathway plays a fundamental role in cell proliferation and survival human tumorigenesis, including gastric cancer. PIK3CA mutations amplification are two major causes of overactivation this cancers. However, until work, there was no sound investigation on the association with clinical outcome cancer, particularly latter. Methods Using direct sequencing real-time quantitative PCR, we examined amplification, their clinicopathological...
Thymoquinone (TQ), the major active compound isolated from black seed oil (Nigella sativa), has been reported to exhibit anti-inflammatory and anticancer abilities. However, exact molecular mechanism underlying effect of TQ is still poorly understood, especially in regard TQ's on endoplasmic reticulum stress-mediated apoptosis.The cytotoxicity T24 253J bladder cancer cells was analyzed by MTT assay, colony formation assay Annexin V-FITC/PI staining, while SV-HUC-1, SV-40 immortalized human...
Neural stem/progenitor cells (NSPCs) are an important source of for cell replacement therapy after nerve injury. How to induce NSPCs differentiation towards neurons and oligodendrocytes is a challenging issue in neuroscience research. In the present study, we polarized microglia into M1 M2 phenotype, used their supernatants differentiation, investigated effects different phenotypes on mechanisms. We discovered that, exposure phenotype supernatant, differentiated fewer Tuj-1+ Olig2+ cells,...
Generally, the emergence of Internet Things enabled applications inspired world during last few years, providing state-of-the-art and novel-based solutions for different problems. This evolutionary field is mainly lead by wireless sensor network, radio frequency identification, smart mobile technologies. Among others, IoT plays a key role in form medical devices wearables, with ability to collect varied longitudinal patient-generated health data, at same time also offering preliminary...
The C allele of rs11136000 variant in the clusterin (CLU) gene represents third strongest known genetic risk factor for late-onset Alzheimer's disease. However, whether this single-nucleotide polymorphism (SNP) is functional and what underlying mechanisms are remain unclear. In study, CLU SNP identified as a by small-scale CRISPR-Cas9 screen. Astrocytes derived from isogenic induced pluripotent stem cells (iPSCs) carrying "C" or "T" exhibit different expression levels. TAR DNA-binding...
We tested the effect of vagus nerve stimulation in endotoxin-induced intestinal tight junction injury mice challenged with lipopolysaccharide (LPS) and examined role α7 nicotinic acetylcholine receptors (α7nAchR) this process. Endotoxemia was induced by intraperitoneal injection LPS (10 mg/kg) male Balb/c mice. Samples were collected 12 h after treatment. associated barrier dysfunction, as evidenced increased amount fluorescein isothiocyanate–dextran circulation. Western blot...
The recurrence of bladder cancer after surgery with or without chemotherapy remains a major challenge in treatment. Previous studies have shown that transient receptor potential vanilloid 1 (TRPV1) acts as tumor suppressor through inducing apoptosis cells. However, whether activation TRPV1 has any synergistic effects pirarubicin (THP), one main drugs used urinary instillation to improve chemotherapeutic efficacy remained elusive. present study verified was differentially expressed cell...
Tetrandrine (TET), a traditional Chinese medicine, exerts remarkable anticancer activity on various cancer cells. However, little is known about the effect of TET human prostate cells, and mechanism function has not yet been elucidated. To investigate effects suppression proliferation, induction apoptosis, inhibition migration invasion in cell lines, DU145 PC-3. Inhibition growth was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay clone formation assay, flow...
Renal cell carcinoma (RCC) is known as one of the most lethal malignancies in urological system because its high incidence metastasis. Tetrandrine (Tet), a traditional Chinese herbal medicine, exerts potent anti-cancer effect variety cancer cells. However, anti-metastatic Tet and possible mechanism RCC still unclear. The present study revealed that significantly suppressed migration invasion 786-O 769-P cells vitro. Mechanistically, protein levels matrix metalloproteinases 9 (MMP-9),...
The protein tyrosine phosphatase 1B (PTP1B), a non-transmembrane phosphatase, has been implicated in gastric pathogenesis. Several lines of recent evidences have shown that PTP1B is highly amplified breast and prostate cancers. aim this study was to investigate amplification cancer its association with poor prognosis patients, further determine the role tumorigenesis. Our data demonstrated significantly up-regulated tissues as compared matched normal by using quantitative RT-PCR (qRT-PCR)...
Abstract Oxidative stress leads to melanocyte death and has been implicated in the pathogenesis of vitiligo. The nuclear factor, E2‐related factor 2 (Nrf2), is a critical transcription protecting cells from oxidative damage. High‐mobility group box 1 ( HMGB 1) chromatin‐associated protein an extracellular damage‐associated molecular pattern molecule. Extracellular released activated immune cells, necrotic or injured becomes proinflammatory mediator through binding cell‐surface receptors...
Thymoquinone, isolated from the seeds of Nigella sativa, has exhibited antitumor properties in a variety cancer types. However, few studies have investigated effect thymoquinone (TQ) on migration and invasion renal cell carcinoma (RCC). In present study, our results confirmed that TQ significantly inhibited human RCC 769‑P 786‑O lines, as demonstrated by wound healing Transwell assays. Additionally, upregulated expression E‑cadherin downregulated Snail, ZEB1 vimentin at mRNA protein levels...
A novel type of core–shell imprinted nanocomposite was synthesized for selective extraction and detection testosterone (TSTO) in prostate cancer cell samples.
Abstract Visual impairment in diabetes is a growing public health concern. Apart from the well‐defined diabetic retinopathy, disturbed optic nerve function, which dependent on myelin sheath, has recently been recognized as an early feature of visual diabetes. However, underlying cellular mechanisms remain unclear. Using streptozotocin‐induced mouse model, we observed deficiency along with composition oligodendroglial lineage cells nerve. We found that new deposition, continuous process lasts...
CD82 acts as a tumor suppressor in series of steps malignant progression. Here, we identified novel function on posttranslational regulating E-cadherin prostate cancer. In our study, the declined expression was verified cancer tissues and cell lines compared with normal tissue lines. Functionally, inhibited migration cleavage from membrane cell. Further study proved that disintegrin metalloproteinase ADAM17 an executor mediated inhibitory regulation shedding Specifically, interacted its...
Comparison of survival between adolescent and young adult vs older patients with hepatocellular carcinoma
Abstract Backgrounds: Nei endonuclease VIII-like 3 (NEIL3), a novel tumor-related gene, was differentially expressed and involved in pathophysiological processes multiple tumors. However, the potential biological functions molecular mechanisms of NEIL3 human clear cell renal carcinoma (ccRCC) have not been identified. Methods The expression pattern prognostic value ccRCC patients were analyzed comprehensive databases validated by qRT-PCR, western blotting analysis, immunohistochemistry,...
Ischemic stroke remains the predominant cause of mortality and functional impairment among adult populations globally. Only a minority ischemic patients are eligible to receive intravascular thrombolysis or mechanical thrombectomy therapy within optimal time window. Among those survivors, around two-thirds suffer neurological dysfunctions over an extended period. Establishing stable repeatable experimental model is extremely significant for further investigating pathophysiological mechanisms...
Intravenous thrombolysis is the only treatment that has evidence of evidence-based medicine in acute ischemic stroke. However, narrow time window limited opporttmities many patients. Transcranial ultrasound a very promising thrombolysis-assisted method, and transcranial plus microbubble- assisted research hotspot recent years. At present, more suitable ultrasonic instruments for number ways ultrasound-assisted are being developed. Key words: stroke; thrombolytic therapy; ...