A5101499282- Hepatitis B Virus Studies
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- interferon and immune responses
- Retinal Diseases and Treatments
- Viral Infections and Vectors
- Antibiotic Resistance in Bacteria
- HIV Research and Treatment
- Phosphodiesterase function and regulation
- Adipokines, Inflammation, and Metabolic Diseases
- Advanced biosensing and bioanalysis techniques
- Retinal and Optic Conditions
- Drug Transport and Resistance Mechanisms
- Cholinesterase and Neurodegenerative Diseases
- Immunotherapy and Immune Responses
- Advanced Cellulose Research Studies
- Cerebral Venous Sinus Thrombosis
- Influenza Virus Research Studies
- Advanced Nanomaterials in Catalysis
- MXene and MAX Phase Materials
- Biological Activity of Diterpenoids and Biflavonoids
- Bone Metabolism and Diseases
- RNA regulation and disease
- Bacterial biofilms and quorum sensing
- Glaucoma and retinal disorders
Third Affiliated Hospital of Sun Yat-sen University
2014-2024
Sun Yat-sen University
2014-2024
Guizhou University
2024
Anhui Provincial Hospital
2012
Anhui Medical University
2012
The Fourth People's Hospital
2010
Fourth People's Hospital of Shenzhen
2010
In the past 50 years there have been considerable efforts to identify cellular receptor of hepatitis B virus (HBV). Recently, in vitro evidence from several groups has shown that sodium–taurocholate cotransporting polypeptide (NTCP, which is encoded by SLC10A1 and transports bile acids into hepatic cells enterohepatic recirculation) a strong candidate. particular, p.Ser267Phe variation results loss HBV function. We tested role NTCP as for chronic patients using genetic association study....
Acetaminophen (APAP) overdose is a leading cause of drug-induced liver injury and acute failure, while the detection, prognosis prediction, therapy for APAP-induced (AILI) remain improved. Here, it determined that temporal pattern circulating cell-free DNA (cfDNA) strongly associated with damage inflammation parameters in AILI. CfDNA comparable to alanine aminotransferase (ALT) predicting mortality outperformed ALT when combined The depletion cfDNA or neutrophils alleviates damage, addition...
Hepatitis B virus (HBV) can encode microRNA-HBV-miR-3, which be detected in both HBV-infected cell lines and peripheral blood exosomes of chronic hepatitis (CHB) patients. This study was conducted to further evaluate its relationship with the current viral markers their dynamics during antiviral therapy.We used Stem-loop real time quantitative PCR (RT-qPCR) quantify HBV-miR-3 serum CHB patients by extracting using Supbio exosome separation kit designing primers Taqman probes specific for...
Objectives: To investigate the damage to retinal nerve fiber layer (RNFL) and ganglion cell complex (GCL+) in diabetic patients without microangioma determine kind of more likely indicate early injury. Subjects methods: We included 360 (360 eyes) with type 2 diabetes mellitus 168 healthy volunteers (168 eyes). Patients were excluded by fundus fluorescein angiography (FFA). The parameters around optic disc macular area measured optical coherence tomography (OCT). Results: peripapillary RNFL...
Abstract Background and Aim The herpes virus entry mediator (HVEM) network has become new directions in targeting novel checkpoint inhibitors for cancer therapy. However, the changes of membrane‐bound HVEM (mHVEM) soluble (sHVEM) hepatocellular carcinoma (HCC) are not fully understood. This study aims to mHVEM sHVEM HCC patients. Methods Serum samples were collected from 65 patients, which levels examined by enzyme‐linked immunosorbent assay. Expressions on peripheral lymphocytes 20 patients...
The purpose of the current study was to identify novel mutations in FRMD7 (FERM domain containing 7) gene and characterize clinical features Chinese patients with congenital motor nystagmus. For this purpose, 18 nystagmus were selected from ocular genetic diseases bank Pediatric Genetic Clinic Zhongshan Ophthalmic Center (Guangdong, China). Direct sequencing used analyze exons adjacent introns gene. heteroduplex‑single strand conformation polypeptide method 96 unrelated normal controls...
Chronic hepatitis B (CHB) and metabolic associated fatty liver disease (MAFLD) are both important public health problems. The effect of concomitant MAFLD on patients with CHB is still unclear. This study aimed to explore the influence fibrosis inflammation in different e antigen (HBeAg) status.We retrospectively collected clinical data 399 treatment-naïve who underwent biopsy. All were divided into two groups (HBeAg± group). Logistic regression analysis was used identify factors inflammatory...
Tenofovir alafenamide (TAF) has been available in China for a short time, little is known about its safety and efficacy patients with hepatitis B virus (HBV)-related acute-on-chronic liver failure (HBV-ACLF). We conducted this study to further verify the of TAF these patients. Eighty-eight eligible subjects were included divided into three groups: group, TDF group ETV group. Clinical laboratory test results collected survival status, suppression status renal function improvement observed...
Background and Aims . We aimed to ascertain the feasibility safety of NA cessation, status patients after predictive factors for relapse subsequent retreatment. Methods A total 92 were enrolled in this prospective study. Patients monitored every month first 3 months cessation thereafter. Results Sixty-two finished 48 weeks follow-up. None died or developed liver failure, cirrhosis, HCC. The 62 could be divided into 4 categories according 48-week clinical development relapse. Virologic...
Zinc finger antiviral protein (ZAP) has been reported to be expressed in hepatocellular carcinoma (HCC), and ZAP expression is associated with apoptotic signaling cancer cells. This study aimed at investigating the of HCC cells its significance clinical pathology.Real-time quantitative PCR western blot assays were employed detect RNA normal human hepatocytes, cells, five primary cell lines. Immunohistochemistry was performed 147 paraffin-embedded tissues adjacent tissues. The analyzed tissue...
Introduction This study is aimed at the determination of effect immune-regulatory factor NLRX1 on antiviral activity hepatocytes against an external stimuli favoring hepatitis B virus infection, and to explore its mechanism action. Methods A HepG2-NTCP model was established using LV003 lentivirus. Cells were transfected overexpression vector siRNA achieve interference expression (OV-NLRX1, si-NLRX1). Levels HBsAg HBcAg determined Western blotting analysis immunohistochemical analysis. The...
NOD-like receptor X1 (NLRX1) is known for its unique mitochondrial localization and plays a negative role in innate immunity. The initial characterization function of chicken NLRX1 remain unclear. Here, mitochondrial-targeted (chNLRX1) protein was identified. It had relatively conserved domains, N-terminal "X" mitochondrial-targeting domain (MT) 2 highly motifs at positions 510-520 412-421. Furthermore, chNLRX1 53aa N-terminus-MT consistent with to mitochondria. Additionally, observed reduce...
Aim . To evaluate the diagnostic value of systematic ophthalmologic imaging examination in diagnosis embedded optic disc drusen (ODD) adolescents with mild visual impairment. Methods Eleven patients were evaluated through optometric examination, fundus photography, field inspection, optical coherence tomography (OCT), ultrasonography (US), and fluorescein angiography (FFA). Of 11 patients, three also underwent cranial orbital magnetic resonance (MRI). Results All had either no apparent...
Abstract Transmembrane 2 (TMEM2) gene inhibits chronic hepatitis-B virus (HBV) infection, while the underlying molecular mechanisms remain unknown. Transcriptome alterations in HepG2 cells following TMEM2 overexpression or silencing by shRNA were analyzed next-generation sequencing. Both and knockdown of caused wide-spread changes expression cells. Differentially expressed genes altered associated with multiple biological processes linked viral infection various signaling pathways. KEGG...
Aim: TMEM2 has been demonstrated to suppress HBV infection by activating the Jak/STAT pathway. In this study, we sought explore mechanism which effects on Zika virus (ZIKV) replication. Materials & methods: was overexpressed. Selected gene mRNA, p-STAT1 levels and interferon stimulated response element activity were examined qRT-PCR, Western blot luciferase assay respectively. Results: Overexpression of significantly inhibited ZIKV replication, upregulated MDA5 RIG-I expression, increased...
Objective: To study the metabolite composition from Serissa japonica, explore substances that play a role in this medicine, and lay foundation for subsequent pharmacological research. Methods: The different metabolites japonica (Thunb.) Thunb. were detected by UPLC-MS/MS technology. Results: findings indicated total 719 differential Thunb., which are divided into 12 categories, including amino acids derivatives, phenolic acids, nucleotides flavonoids, quinones, lignans coumarins, tannins,...
Antiviral therapy is essential for hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF). No data are available on the long-term prognosis or safety of tenofovir alafenamide (TAF), disoproxil fumarate (TDF), entecavir (ETV) in treating HBV-ACLF globally. This study was conducted to investigate efficacy and three nucleos(t)ide analogs treatment HBV-ACLF. In this prospective, real-world cohort study, patients with were assigned TAF, TDF, ETV groups. A total 199 completed 144-week...
Tyrosinase is a bifunctional enzyme which oxidizes the initial step of melanin biosynthesis, that is, conversion tyrosine to dopa and subsequently dopaquinone. It glycosylated protein major regulator melanogenesis. To date, many approaches have been tried regulate tyrosinase activity content. end, we screened small interfering RNA sequences for sequence-inhibited expression in B16 cells C57BL/6 mice. We analyzed mRNA levels by quantitative real-time PCR determined content at 24, 48, 72 hours...