A5101825846- Antibiotic Resistance in Bacteria
- Cancer-related gene regulation
- Antimicrobial Resistance in Staphylococcus
- Microbial Inactivation Methods
- Horticultural and Viticultural Research
- Autophagy in Disease and Therapy
- Gene Regulatory Network Analysis
- Plant biochemistry and biosynthesis
- Immunotherapy and Immune Responses
- Circular RNAs in diseases
- Antibiotics Pharmacokinetics and Efficacy
- Plant Gene Expression Analysis
- Immune cells in cancer
- Clostridium difficile and Clostridium perfringens research
- Bacterial Identification and Susceptibility Testing
- Ubiquitin and proteasome pathways
- CRISPR and Genetic Engineering
- Liver Disease Diagnosis and Treatment
- Fermentation and Sensory Analysis
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Advanced Proteomics Techniques and Applications
- Cancer-related Molecular Pathways
- Wnt/β-catenin signaling in development and cancer
Renji Hospital
2021-2024
Shanghai Jiao Tong University
2020-2024
First Affiliated Hospital Zhejiang University
2016-2024
Zhejiang Wanli University
2021-2024
Zhejiang University
2016-2023
Blood Center of Zhejiang Province
2015-2022
Chinese Academy of Medical Sciences & Peking Union Medical College
2022
Northeastern University
2017-2021
Huazhong University of Science and Technology
2021
Northeast Forestry University
2021
Tumor‐associated macrophages (TAMs) are recognized as antitumor suppressors, but how TAMs behave in the hypoxic environment of hepatocellular carcinoma (HCC) remains unclear. Here, we demonstrated that hypoxia inducible factor 1α induced increased expression triggering receptor expressed on myeloid cells‐1 (TREM‐1) TAMs, resulting immunosuppression. Specifically, TREM‐1‐positive (TREM‐1 + ) abundant at advanced stages HCC progression indirectly impaired cytotoxic functions CD8 T cells and...
Multi-drug resistance is the major cause of chemotherapy failure in hepatocellular carcinoma (HCC). YAP, a critical effector Hippo pathway, has been shown to contribute progression, metastasis and invasion cancers. However, potential role YAP mediating drug remains obscure.RT-qPCR western blot were used assess expression HCC cell lines. CCK-8 assays, flow cytometry, xenograft tumour model, immunochemistry GFP-mRFP-LC3 fusion proteins utilized evaluate effect on multi-drug resistance,...
The decrease of microRNA-452 (miR-452) in gliomas promoted stem-like features and tumorigenesis. However, the role miR-452, especially regulating cancer stem cells (CSCs) hepatocellular carcinoma (HCC) remains ambiguous. We enriched HCC by serial passages hepatospheres with chemotherapeutic agents. Stem-like characteristics including capabilities chemo-resistance, stemness-related gene expression profiling, self-renewal, tumorigenicity metastasis formation were detected. MiR-452 was markedly...
Holliday junction recognition protein (HJURP) has been implicated in many cancers including hepatocellular carcinoma (HCC). However, the underlying mechanism by which HJURP promotes HCC cell proliferation remains unclear.RT-qPCR and immunohistochemistry were used to detect expression adjacent tumor tissues lines. The localization of p21 determined immunofluorescence western blot. Co-immunoprecipitation blot validate stability signaling pathways affected HJURP. effects on assessed both vivo...
Staphylococcus aureus (SA) is a relatively uncommon cause of urinary tract infections (UTIs) in the general population. Although rare, S. aureus-induced UTIs are prone to potentially life-threatening invasive such as bacteremia. To investigate molecular epidemiology, phenotypic characteristics, and pathophysiology UTIs, we analyzed non-repetitive 4,405 isolates collected from various clinical sources 2008 2020 hospital Shanghai, China. Among these, 193 (4.38%) were cultivated midstream urine...
Background & Aims: Regulatory T cells (Tregs) are an obstacle to PD-1 blockade-mediated antitumor efficacy. However, the behaviors of Tregs response anti-PD-1 in HCC and characteristics tissue adaptation from peripheral lymphoid tissues tumor still unclear. Approach Results: Here, we determine that monotherapy potentially augments accumulation CD4 + Tregs. Mechanistically, mediates proliferation rather than tumor. Increased burden replenishes intratumoral Tregs, raising ratio CD8 cells....
Plant homeodomain finger protein 8 (PHF8) serves an activator of epithelial-mesenchymal transition (EMT) and is implicated in various tumors. However, little known about PHF8 roles hepatocellular carcinoma (HCC) regulating E-cadherin expression.PHF8 expression pattern was investigated by informatic analysis verified RT-qPCR immunochemistry HCC tissues cell lines. CCK8, xenograft tumor model, transwell assay, tandem mCherry-GFP-LC3 fusion assay were utilized to assess the effects on...
Holliday Junction Recognition Protein (HJURP) is involved in various cancers including hepatocellular carcinoma (HCC).Current studies have showed that HJURP correlated with HCC proliferation.However, the role of Epithelial-to-Mesenchymal Transition remains unclear.In this study, we found knockdown significantly reduced migration and invasion abilities cells both vivo vitro by interacting Sphingosine kinase1 (SPHK1).Conversely, overexpression enhanced these biological abilities.Moreover, high...
Bacterial membrane vesicles (MVs) have recently gained much attention and been shown to carry a wide diversity of secreted bacterial components. However, it is poorly understood whether MV carriage an indispensable requirement for cargo's function. Bacteriocins as weapons warfare shape the composition microbial communities. Many bacteriocins pronounced hydrophobicity that imposed by their mechanism action, but how they diffuse through aqueous environments reach target competitors not known....
Abstract Background Monoterpenes are among the most important volatile aromatic compounds contributing to flavor and aroma of grapes wine. However, molecular basis monoterpene biosynthesis has not yet been fully elucidated. Results In our study, transcriptomics gas chromatography-mass spectrometry (GC-MS) were used mine candidate genes transcription factors involved in between high-monoterpene zero-monoterpene table grape cultivars. We found that was positively correlated by expression five...
Abstract Diverse post-translational modifications have been shown to play important roles in regulating protein function eukaryotes. By contrast, the of bacteria are not so well understood, particularly as they relate pathogenesis. Here, we demonstrate modification by covalent addition lactate lysine residues (lactylation) human pathogen Staphylococcus aureus . Lactylation is dependent on concentration and specifically affects alpha-toxin, which a single lactylated required for full activity...
Starting in the early 2000s, sophisticated technologies have been developed for rational construction of synthetic genetic networks that implement specified logical functionalities. Despite impressive progress, however, scaling necessary order to achieve greater computational power has hampered by many constraints, including repressor toxicity and lack large sets mutually orthogonal repressors. As a consequence, typical circuit contains no more than roughly seven repressor-based gates per...
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Nanosecond pulsed electric fields (nsPEFs) is emerged as a potential curative modality to ablate hepatocellular carcinoma (HCC). The application of local ablation usually limited by insufficiency liver function. While baicalin, flavonoid isolated from Scutellaria baicalensis Georgi, has been proven possess both anti-tumor and protective effects. Our study aimed estimate different responses hepatic cancer cells hepatocytes the combination nsPEFs baicalin. Cell viability, apoptosis necrosis,...
Many proteoforms-arising from alternative splicing, post-translational modifications (PTM), or paralogous genes-have distinct biological functions, such as histone PTM proteoforms. However, their quantification by existing bottom-up mass-spectrometry (MS) methods is undermined peptide-specific biases. To avoid these biases, we developed and implemented a first-principles model (HI
Aroma is one of the most important indicators grape quality. Girdling and foliar fertilization with K (K2O) are common agronomic practices applied to improve berry quality in production. However, little known about its effect on accumulation biosynthesis entire aromatic profile. Our study was aimed explore influences girdling (alone or combination) general properties, phenolic composition, volatile free aroma compounds, spatial temporal expression terpene-related genes sensory properties...
To investigate the potential oncogene promoting recurrence of hepatocellular carcinoma (HCC) following liver transplantation (LT), throughput RNA sequencing was performed in a subgroup HCC patients. The up-regulated FAM83D tissues found and further verified 150 patients by real-time PCR immunohistochemistry. overexpression significantly correlated with high rate LT poor characteristics such as AFP, differentiation. Of cancer stem cells (CSCs) markers, CD44 expression effectively suppressed...
Abstract Objectives Fosfomycin resistance has become a clinical concern. In this study, we analysed the dynamic change of fosfomycin MIC in epidemic Staphylococcus aureus lineages teaching hospital Shanghai for 12 years and sought to elucidate major underlying mechanisms. Methods MLST was conducted 4580 S. isolates recovered from 2008 2019. determined by agar dilution method. The genome data 230 lineage were acquired next-generation sequencing (NGS) platform. Gene deletion corresponding...