A5072885240- Cervical and Thoracic Myelopathy
- Spine and Intervertebral Disc Pathology
- Heterotopic Ossification and Related Conditions
- Bone health and osteoporosis research
- Spinal Fractures and Fixation Techniques
- Bone health and treatments
- TGF-β signaling in diseases
- Hip and Femur Fractures
- Cancer-related molecular mechanisms research
- Bone Metabolism and Diseases
- Cancer Diagnosis and Treatment
- Spinal Cord Injury Research
- Connective tissue disorders research
- Congenital Diaphragmatic Hernia Studies
- Kruppel-like factors research
- Sirtuins and Resveratrol in Medicine
- Genomics, phytochemicals, and oxidative stress
- Bone Tissue Engineering Materials
- Liver Disease Diagnosis and Treatment
- Spinal Hematomas and Complications
- Heat shock proteins research
- Endometriosis Research and Treatment
- Nanoparticle-Based Drug Delivery
- Developmental Biology and Gene Regulation
- Hepatocellular Carcinoma Treatment and Prognosis
Peking University
2014-2024
Peking University Third Hospital
2014-2024
Ministry of Education of the People's Republic of China
2021-2024
Anhui Business College
2023-2024
Beijing Haidian Hospital
2020
First Affiliated Hospital of Bengbu Medical College
2017-2019
Abstract The autonomic nervous system plays a crucial role in regulating bone metabolism, with sympathetic activation stimulating resorption and inhibiting formation. We found that fractures lead to increased tone, enhanced osteoclast resorption, decreased osteoblast formation, thus hastened systemic loss ovariectomized (OVX) mice. However, the combined administration of parathyroid hormone (PTH) β-receptor blocker propranolol dramatically promoted formation osteoporotic fracture healing OVX...
AIB1, a member of the steroid receptor coactivator (SRC) family that participates in gene transcriptional activation by nuclear receptors and other transcription factors, is required for animal growth reproductive development implicated breast carcinogenesis. The mechanisms underlying AIB1 pleiotropic functions are not fully understood neither regulation its activity. Here, we showed was sumoylated protein sumoylation attenuated transactivation activity which contrast to paralogs, GRIP1...
Ossification of the ligamentum flavum (OLF) is a disorder heterotopic ossification spinal ligaments and main cause thoracic canal stenosis. Previous studies suggested that miR-132-3p negatively regulates osteoblast differentiation. However, whether involved in process OLF has not been investigated. In this study, we investigated effect its target genes forkhead box O1 (FOXO1), growth differentiation factor 5 (GDF5) SRY-box 6 (SOX6) on osteogenic (LF) cells. We demonstrated was down-regulated...
Oxeiptosis is a reactive oxygen species (ROS)-induced pathway of cell death. The involvement circular RNAs (circRNAs) has been confirmed in the incidence and progression intervertebral disc degeneration (IVDD). However, whether oxeiptosis occurs IVDD how circRNAs regulate still unclear. In this study, we discovered that could be induced nucleus pulposus cells (NPCs), circFOXO3 was significantly upregulated after induction. Transfection using small interfering RNA (siRNA) inhibited NPCs....
Abstract Intervertebral disc degeneration (IDD) is an important pathological basis for degenerative spinal diseases and involved in mitophagy dysfunction. However, the molecular mechanisms underlying regulation IDD remain unclear. This study aimed to clarify role of DJ-1 regulating during pathogenesis. Here, we showed that mitochondrial localization nucleus pulposus cells (NPCs) first increased then decreased response oxidative stress. Subsequently, loss- gain-of-function experiments...
Low-surface-density streams are important tracers to study the formation and evolution of Milky Way. Using accurate astrometric measurements from Gaia mission, we discover a low-surface-density stream in northern hemisphere, with length ∼110 degree width $1.23$ kpc. The vertical velocity dispersion perpendicular is around $22.4$ km s^-1. spectral data member candidate stars LAMOST DESI shows large metallicity range -1.8 -0.7. Based on those properties, claim that originated dwarf galaxy....
Obesity is a risk factor for thoracic ossification of ligament flavum (TOLF) that characterized by ectopic bone formation in the spinal ligaments. Hyperleptinemia common feature obese people, and leptin, an adipocyte-derived cytokine with proliferative osteogenic effects several cell types, believed to be important pathogenesis TOLF. However, how leptin might stimulate differentiation TOLF not totally understood. We reported here leptin-induced effect cells associated activation signaling...
Abstract Ossification of the ligamentum flavum (OLF) is a pathology almost only reported in East Asian countries. The leading cause OLF thoracic spinal canal stenosis and myelopathy. In this study, role miR‐199b‐5p jagged 1 (JAG1) primary cell osteogenesis was examined. MiR‐199b‐5p found to be down‐regulated during osteogenic differentiation cells, while overexpression inhibited differentiation. addition, JAG1 up‐regulated knockdown via RNA interference caused an inhibition Notch signalling...
Mechanical stress is considered to be an important factor in the progression of thoracic ossification ligament flavum (TOLF). To elucidate mechanism underlying mechanical stress-induced TOLF, we investigated effect stretching on cultured cells derived from TOLF and non-TOLF patients. We found that mRNA expression alkaline phosphatase (ALP), osteocalcin, Runx2, osterix, but not Dlx5 Msx2, was significantly increased by cells. In addition, seems finely tuned stretching-triggered activation...
Mechanical stress and genetic factors play important roles in the occurrence of thoracic ossification ligament flavum (TOLF), which can occur at one, two, or multiple levels spine. It is unclear whether single- multiple-level TOLF differ terms osteogenic differentiation potency osteogenesis-related gene expression under cyclic mechanical stress. This was addressed present study using patients with non‑TOLF single‑ multiple‑level (n=8 per group). Primary cells were cultured osteogenesis...
ABSTRACT This study investigated the pathological process of Notch signaling in osteogenesis ligamentum flavum tissues and cells, associated regulatory mechanisms. receptors, ligands, target genes were identified by quantitative polymerase chain reaction (qPCR) cells immunohistochemistry sections from ossification (OLF) patients controls. The temporospatial expression patterns JAG1/Notch2/HES1 human during osteogenic differentiation determined qPCR. Lentiviral vectors for Notch2...
Background Ossification of ligamentum flavum (OLF) is an insidious and debilitating heterotopic ossifying disease with etiological heterogeneity undefined pathogenesis. Obese individuals predispose to OLF, whereas the underlying connections between obesity phenotype OLF pathomechanism are not fully understood. Therefore, this study aims explore distinct obesity-related genes their functional signatures in OLF. Methods The transcriptome sequencing data related were downloaded from GSE106253...
Thoracic ossification of the ligamentum flavum (TOLF) is a rare heterotopic spinal ligaments, which major cause thoracic canal stenosis and myelopathy.In this study, roles miR-490-3p forkhead box O1 (FOXO1) in osteogenesis human cells were investigated.MiR-490-3p was found to be down-regulated during osteogenic differentiation cells, while their overexpression inhibited differentiation.In addition, analysis target prediction dual luciferase reporter assays supported that directly targeted...
A basic experimental study.To elucidate the role and mechanism of interleukin (IL)-17A in thoracic ossification ligamentum flavum (TOLF).TOLF is characterized by replacement with ossified tissue one leading causes spinal stenosis. IL-17A an important member IL-17 family that has received widespread attention for its key contributions to regulation bone metabolism heterotopic ossification. However, it unclear whether involved TOLF.Cell counting kit-8 assay 5-ethynyl-2'-deoxyuridine staining...