A5079819963- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Cancer, Hypoxia, and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer Immunotherapy and Biomarkers
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Cancer Research and Treatments
- Toxoplasma gondii Research Studies
- interferon and immune responses
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Architecture and Cultural Influences
- Cellular Mechanics and Interactions
- Extracellular vesicles in disease
- vaccines and immunoinformatics approaches
- Multiple Sclerosis Research Studies
- Ferroptosis and cancer prognosis
University of Calgary
2016-2024
Icahn School of Medicine at Mount Sinai
2023-2024
Mount Sinai Health System
2024
Cancer Institute (WIA)
2024
Tisch Cancer Institute
2024
Tehran University of Medical Sciences
2011-2020
Ontario Brain Institute
2018-2020
Allen Institute for Brain Science
2019
University of Tehran
2014-2018
Islamic Azad University of Birjand
2013
Abstract Remyelination following CNS demyelination restores rapid signal propagation and protects axons; however, its efficiency declines with increasing age. Both intrinsic changes in the oligodendrocyte progenitor cell population extrinsic factors lesion microenvironment of older subjects contribute to this decline. Microglia monocyte-derived macrophages are critical for successful remyelination, releasing growth clearing inhibitory myelin debris. Several studies have implicated delayed...
The migration of leukocytes into the CNS drives neuropathology multiple sclerosis (MS). This penetration likely utilizes energy resources that remain to be defined. Using experimental autoimmune encephalomyelitis (EAE) model MS, we determined macrophages within perivascular cuff post-capillary venules are highly glycolytic as manifested by strong expression lactate dehydrogenase A (LDHA) converts pyruvate lactate. These expressed prominent levels monocarboxylate transporter-4 (MCT-4)...
Microglia and macrophages are the largest component of inflammatory infiltrate in glioblastoma (GBM). However, whether there differences their representation activity prognostically-favorable isocitrate dehydrogenase (IDH)-mutated compared to -wild type GBMs is unknown. Studies on human specimens untreated IDH-mutant rare given they comprise 10% all often present at lower grades, receiving treatments prior dedifferentiation that can drastically alter microglia macrophage phenotypes. We were...
Oncogenic signaling by NOTCH is elevated in brain tumor-initiating cells (BTIC) malignant glioma, but the mechanism of its activation unknown. Here we provide evidence that tenascin-C (TNC), an extracellular matrix protein prominent increases activity BTIC to promote their growth. We demonstrate proximal localization TNC and human glioblastoma specimens orthotopic murine xenografts implanted intracranially. In tissue culture, was superior amongst several proteins enhancing sphere-forming...
Background Inflammation-exacerbated secondary brain injury and limited tissue regeneration are barriers to favourable prognosis after intracerebral haemorrhage (ICH). As a regulator of inflammation lipid metabolism, Liver X receptor (LXR) has the potential alter microglia/macrophage (M/M) phenotype, assist repair by promoting cholesterol efflux recycling from phagocytes. To support clinical translation, benefits enhanced LXR signalling examined in experimental ICH. Methods...
Dendritic cells are important in initiating immune responses; therefore, a range of dendritic cell–based approaches have been established to induce response against cancer cells. However, the presence immunosuppressive mediators such as adenosine tumor microenvironment reduces efficacy immunotherapy. In this study, we investigated whether blockade A2A receptor with selective antagonist and CD73 inhibitor may increase vaccine. According findings, therapeutic combination reduced growth,...
By rejuvenating the deficient activity of myeloid cells in glioblastoma, niacin improves treatment otherwise incurable brain tumors.
Glioblastomas (GBM) are aggressive brain tumors with extensive intratumoral heterogeneity that contributes to treatment resistance. Spatial characterization of GBMs could provide insights into the role tumor microenvironment in regulating heterogeneity. Here, we performed spatial transcriptomic and single-cell analyses mouse human GBM dissect impact distinct anatomical regions brains on GBM. In a syngeneic model, transcriptomics revealed numerous extracellular matrix (ECM) molecules,...
Lactobacillus crispatus is one of the most predominant species in healthy vagina microbiota. Nevertheless, interactions between this commensal bacterium and immune system are largely unknown. Given importance dendritic cells ( DC s) regulation immunity, study was performed to elucidate influence vaginal isolated L. SJ ‐3C‐ US from Iranian women on s, either directly by exposure s ultraviolet‐inactivated UVI ) heat‐killed HK or indirectly its cell‐free supernatant CFS ), outcomes response. In...
PD-1 on glioblastoma cells promotes brain tumor growth, and therapeutic antibodies cannot suppress its proliferative effects.
Abstract Glioblastomas (GBMs) are highly aggressive, recurrent, and lethal brain tumors that maintained via tumor‐initiating cells (BTICs). The aggressiveness of BTICs may be dependent on the extracellular matrix (ECM) molecules enriched within GBM microenvironment. Here, we investigated expression ECM in patients by mining transcriptomic databases also staining human specimens. RNA levels for fibronectin, brevican, versican, heparan sulfate proteoglycan 2 (HSPG2), several laminins were high...
Different cells and mediators in the tumor microenvironment play important roles progression of breast cancer. The aim this study was to determine composition during order discover new related biomarkers potentials for targeted therapy.In study, cancer biopsies from four different stages, control were collected. Then, mRNA expression several markers CD4+ T cell subsets including regulatory (Treg), helper (Th) type 1, 2 17 determined. In addition, we investigated two inflammatory cytokines...
Background Capparis spinosa, Rosa canina, Securidaca securigera, Silybum marianum, Urtica dioica, Trigonella foenum-graecum and Vaccinium arctostaphylos are used traditionally as an herbal combination for treatment of diabetic patients in Iran. Despite the clinical evidence supporting their use solitary form, no controlled human study has determined efficacy safety patients.Methods A total 150 type II both sexes under oral anti-hyperglycemic drugs (maximum 10 mg glyburide 1000 metformin...
Context: Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by autoreactive antibodies. Recent findings revealed the importance of innate immune responses, especially Toll-like receptors (TLRs) in pathogenesis SLE.Objective: In this study, level TLR9 expression on peripheral blood mononuclear cells (PBMCs) was analyzed. The levels produced IFN-α were also measured supernatant PBMCs from SLE patients and healthy controls after stimulation with CpG ODN2216 which...
Imagining ways to prevent or treat glioblastoma (GBM) has been hindered by a lack of understanding its pathogenesis. Although overexpression platelet derived growth factor with two A-chains (PDGF-AA) may be an early event, critical details the core biology GBM are lacking. For example, existing PDGF-driven models replicate microscopic appearance, but not genomic architecture. Here we report model that overcomes this barrier authenticity.Using method developed establish neural stem cell...
Abstract We reported previously that microglia decreased the growth of human brain tumor-initiating cells (BTICs). Through microarray analyses BTICs exposed in vitro to microglia, we found induction several genes ascribed have roles cell cycle arrest, reduced proliferation and differentiation. Herein, tested hypothesis one these genes, arrest specific 1 (Gas1), is a novel reduction factor induced by microglia. increased expression Gas1 transcript protein glioblastoma patient-derived BTIC...
Iron deposition in the brain begins early multiple sclerosis (MS) and continues unabated. Ferrous iron is toxic to neurons, yet therapies used MS do not counter neurotoxicity. Extracts of Hibiscus sabdariffa (HS) are many cultures for medicinal purposes. We collected a distinct HS extract found that it abolished killing neurons by culture; medications were ineffective when similarly tested. Neuroprotection was due chelation or anthocyanin content. In free radical scavenging assays,...