A5041683100- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Drug-Induced Adverse Reactions
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- Endoplasmic Reticulum Stress and Disease
- Pregnancy and Medication Impact
- Heat shock proteins research
- Mast cells and histamine
- Autoimmune Bullous Skin Diseases
- Transgenic Plants and Applications
- Reproductive System and Pregnancy
- Adolescent and Pediatric Healthcare
- Eosinophilic Disorders and Syndromes
- Eosinophilic Esophagitis
- Wireless Sensor Networks for Data Analysis
- Atherosclerosis and Cardiovascular Diseases
- Geriatric Care and Nursing Homes
- Food Allergy and Anaphylaxis Research
- Business Strategy and Innovation
- RNA regulation and disease
- Plant Virus Research Studies
- Complement system in diseases
- Mosquito-borne diseases and control
University of Fribourg
2022
University Hospital of Bern
2012-2021
University of Bern
2020-2021
University of St. Gallen
2008-2012
Kantonsspital St. Gallen
2006-2008
Seiko Holdings (Japan)
2004
ABSTRACT Viral infection of the liver can lead to severe tissue damage when high levels viral replication and spread in organ are coupled with strong induction inflammatory responses. Here we report an unexpected correlation between expression a functional X domain encoded by hepatotropic mouse hepatitis virus strain A59 (MHV-A59), high-level production cytokines, acute mice. X-domain (also called macro domain) proteins possess poly-ADP-ribose binding and/or ADP-ribose-1′′-phosphatase (ADRP)...
Allopurinol (ALP) hypersensitivity is a major cause of severe cutaneous adverse reactions and strongly associated with the HLA-B*58:01 allele. However, it can occur in absence this allele identical clinical manifestations. The immune mechanism ALP-induced poorly understood, T cell-reactivity pattern patients or without not known. To understand interactions among drug, HLA, TCR, we generated cell lines that react to ALP its metabolite oxypurinol (OXP) from HLA-B*58:01(+) HLA-B*58:01(-) donors...
Severe acute respiratory syndrome (SARS) is a zoonotic disease caused by SARS-related coronavirus (SARS-CoV) that emerged in 2002 to become global health concern. Although the original outbreak was controlled classical public measures, there real risk another SARS-CoV could re-emerge from its natural reservoir, either form or as more virulent pathogenic strain; which case, virus would be difficult control absence of any effective antiviral drugs vaccines. Using well-studied isolate...
Several plus-strand RNA viruses encode proteins containing macrodomains. These domains possess ADP-ribose-1″-phosphatase (ADRP) activity and/or bind poly(ADP-ribose), poly(A) or poly(G). The relevance of these activities in the viral life cycle has not yet been resolved. Here, we report that genetically engineered mutants severe acute respiratory syndrome coronavirus (SARS-CoV) and human 229E (HCoV-229E) expressing ADRP-deficient macrodomains displayed an increased sensitivity to antiviral...
The antiretroviral drug abacavir (abc) elicits severe hypersensitivity reactions in HLA ‐ B *5701 + individuals. To understand the abc‐specific activation of CD 8 T cells, we generated ‐cell clones (abc‐ TCC s). Abc reactivity could not be linked to metabolism and/or processing drug, since abc metabolizing enzymes were expressed immune cells and inhibition proteasome APC s did affect reactivity. Ca 2+ influx assays revealed different patterns abc‐ s. While all reacted presented on molecules,...
Abacavir hypersensitivity is a severe reaction which occurs exclusively in carriers of the HLA-B*57∶01 allele. In vitro culture PBMC with abacavir results outgrowth abacavir-reacting CD8+ T cells, release IFNγ and are cytotoxic. How this immune response induced what recognized by these cells still matter debate. We analyzed conditions required to develop an abacavir-dependent cell vitro. The reactivity was independent co-stimulatory signals, as neither DC maturation nor inflammatory...
This study highlights the key role of polycytotoxic CD8 + T cells in severity toxic epidermal necrolysis.
A stringent quality control process selects misfolded polypeptides generated in the endoplasmic reticulum (ER) for ER-associated degradation (ERAD). Here we assessed maintenance of efficient glycoprotein folding cells with defective ERAD caused by lack adaptation intralumenal level ER degradation-enhancing alpha-mannosidase-like protein (EDEM) to an increase cargo load. When these were converted into factories production high levels human beta-secretase, maturation this N-glycosylated...
Adverse drug reactions are one of the leading causes morbidity and mortality in health care worldwide. Human leukocyte antigen (HLA) alleles have been strongly associated with hypersensitivities, causative drugs shown to stimulate specific T cells at sites autoimmune destruction. The structural elements recognized by drug-specific cell receptors (TCRs) vivo poorly defined. Drug-stimulated express TCRs for peptide/HLA complexes, but characteristics peptides (sequence, or endogenous exogenous...
In breastfeeding patients with chronic inflammatory rheumatic diseases, a postpartum flare may require the use of biologics. However, data on safety biologics during lactation are scarce, potentially impeding decision-making process. We report two cases women in whom treatment monoclonal IgG antibody (rituximab or canakinumab) was indicated period. both cases, continued, and drug levels mother's serum, serial breast milk samples infant's serum were measured. Both rituximab canakinumab showed...
Oral Abstracts O1 Functionally distinct HMGB1 isoforms correlate with physiological processes in drug-induced SJS/TEN Daniel F. Carr, Wen-Hung Chung, Rosalind E. Jenkiins, Mas Chaponda, Gospel Nwikue, Elena M. Cornejo Castro, J. Antoine, Munir Pirmohamed O2 Hypersensitivity reactions to beta-lactams, does the t cell recognition pattern influence clinical picture? Natascha Wuillemin, Dolores Dina, Klara K. Eriksson, Yerly O3 Specific binding characteristics of HLA alleles associated...
Malaria affects the poorer regions of world and is tremendous health economic burden for developing countries. Extracellular vesicles (EVs) are small released by almost any cells in human body, including malaria infected red blood cells. Recent evidence shows that EVs might contribute to pathogenesis malaria. In addition, hold considerable value biomarker discovery. However, there still significant gaps our understanding EV biology. So far most knowledge about comes from vitro work. More...
Abstract Rationale: Abacavir (ABC) hypersensitivity is mediated by specific CD8+ T cells that interact with ABC in HLA-B*5701+ individuals. To understand how presented and recognized cells, we generated cell clones (ABC-TCC) analysed the kinetic of recognition. Methods: ABC-TCC from naïve subjects were limiting dilution. TCC activation was measured means Ca2+ influx analyses Annexin V staining. Results: presence autologous antigen-presenting (APC) reacted within a short time (3-25 min) to...
Abstract Rationale: The antiretroviral drug abacavir (abc) elicits a strong CD8-mediated immune response in HLA-B*5701+ subjects. We used this model to study the interaction of small chemicals with system. Methods: generated abc-specific T-cell clones (abc-TCC) from donors and analyzed their specific activation Ca2+ influx assays by annexin V staining. Results: Abc-metabolizing enzymes were absent cells inhibition proteasome antigen presenting did not affect TCC reactivity. Five out 50...