A5012895759- Astrophysics and Cosmic Phenomena
- Spinal Cord Injury Research
- Gamma-ray bursts and supernovae
- MicroRNA in disease regulation
- TGF-β signaling in diseases
- Particle Detector Development and Performance
- Dark Matter and Cosmic Phenomena
- Extracellular vesicles in disease
- Pancreatic and Hepatic Oncology Research
- Immune cells in cancer
- Nerve injury and regeneration
- Bone Metabolism and Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Autophagy in Disease and Therapy
- RNA modifications and cancer
- Spine and Intervertebral Disc Pathology
- Cancer, Hypoxia, and Metabolism
- Hippo pathway signaling and YAP/TAZ
- Cell Adhesion Molecules Research
- Phytochemical Studies and Bioactivities
- Neutrino Physics Research
- Epigenetics and DNA Methylation
- Heat shock proteins research
- Polysaccharides and Plant Cell Walls
- Cancer-related molecular mechanisms research
Nanjing Medical University
2016-2025
Jiangsu Province Hospital
2018-2025
Liaoning University
2024-2025
Jiangnan University
2025
Audie L. Murphy Memorial VA Hospital
2024
Purple Mountain Observatory
2024
Chinese Academy of Sciences
2024
Laboratoire AstroParticule et Cosmologie
2024
Shandong University
2023-2024
Université Paris Cité
2024
Spinal cord injury (SCI) can lead to severe motor and sensory dysfunction with high disability mortality. In recent years, mesenchymal stem cell (MSC)-secreted nano-sized exosomes have shown great potential for promoting functional behavioral recovery following SCI. However, MSCs are usually exposed normoxia in vitro, which differs greatly from the hypoxic micro-environment vivo. Thus, main purpose of this study was determine whether derived under hypoxia (HExos) exhibit greater effects on...
Approximately 10% of bone fractures do not heal satisfactorily, leading to significant clinical and socioeconomic implications. Recently, the role macrophages in regulating marrow stem cell (BMSC) differentiation through osteogenic pathway during fracture healing has attracted much attention. Methods: The tibial monocortical defect model was employed determine critical macrophage scavenger receptor 1 (MSR1) intramembranous ossification (IO) vivo. potential functions mechanisms MSR1 were...
Subtalar osteoarthritis (STOA) is often secondary to chronic ankle sprains, which seriously affects the quality of life patients. Due its etiology and pathogenesis was not studied equivocally yet, there currently a lack effective conservative treatments. Although they have been used for tissue repair, platelet-rich plasma-derived exosomes (PRP-Exo) disadvantage low retention short-lived therapeutic effects. This study aimed determine whether incorporation PRP-Exo in thermosensitive hydrogel...
Abstract Drug delivery systems with high content of drug can minimize excipients administration, reduce side effects, improve therapeutic efficacy and/or promote patient compliance. However, engineering such is extremely challenging, as their loading capacity inherently limited by the compatibility between molecules and carrier materials. To mitigate drug-carrier limitation towards therapeutics encapsulation, we developed a sequential solidification strategy. In this strategy, precisely...
Within mineralized bone, osteocytes form dendritic processes that travel through canaliculi to make contact with other and cells on the bone surface.This three-dimensional syncytium is thought be necessary maintain viability, cell-to-cell communication, mechanosensation.E11/gp38 earliest osteocyte-selective protein expressed as osteoblast differentiates into an osteoid cell or osteocyte, first appearing forming of these cells.Bone extracts contain large amounts E11, but immunostaining only...
Abstract Osteocytes are thought to be mechanosensory cells that respond mechanical stress by sending signals other bone initiate remodeling. An osteocyte-like cell line MLO-Y4 provides a model system examine whether gap junctions participate in the regulation of osteocyte function and signaling stress. In this study, we show coupled junction channels mediate coupling. Biochemical analyses connexin 43 (Cx43) is major protein expressed approximately 5% Cx43 phosphorylated. were exposed using...
BackgroundOsteosarcoma (OS) is a malignant tumor mainly occurring in young people. Due to the limited effective therapeutic strategies, OS patients cannot achieve further survival improvement. G-protein-coupled receptors (GPCRs) constitute largest family of cell membrane and consequently hold significant promise for imaging targeted therapy. We aimed explore biological functions Sphingosine 1-phosphate receptor 3 (S1PR3), one members GPCRs family, possibility S1PR3 as an target treatment...
High-risk human papillomavirus oncoproteins E6 and E7 are the major etiological factors of cervical cancer but insufficient for malignant transformation cancer. Dysregulated alternative splicing, mainly ascribed to aberrant splicing factor levels activities, contributes most hallmarks. However, do regulate expression factors? Does acts as an "accomplice" E6E7 promote progression? Here, we identified that SRSF10, which promotes tumorigenesis cervix, was upregulated by via E2F1 transcriptional...
Disruption of the blood-spinal cord barrier (BSCB) leads to inflammatory cell infiltration and neural death, thus, contributing poor functional recovery after spinal injury (SCI). Previous studies have suggested that Sirtuin 1 (SIRT1), an NAD+-dependent class III histone deacetylase, is abundantly expressed in endothelial cells promotes homeostasis. However, role SIRT1 BSCB function SCI remains poorly defined. Here, we report highly cells, its expression significantly decreases SCI. Using...
Spinal cord injury (SCI) remains a significant health concern, with limited available treatment options. This condition poses medical, economic, and social challenges. SCI is typically categorized into primary secondary injuries. Inflammation, oxidative stress, scar formation, the immune microenvironment impede axon regeneration subsequent functional restoration. Numerous studies have shown that destruction of blood-brain barrier (BBB) microvessels crucial factor in severe injury....
We have observed, in our previous studies, that fluid flow increases gap junction-mediated intercellular coupling and the expression of a junction protein, connexin 43, osteocyte-like MLO-Y4 cells. Interestingly, this stimulation is further enhanced during poststress period, indicating released factor(s) likely to be involved. Here, we report conditioned medium obtained from flow-treated cells increased number functional junctions 43 protein. These changes are similar those observed directly...
Transforming growth factor beta type II receptor (TbetaRII) is a tumor suppressor gene that can be transcriptionally silenced by histone deacetylases (HDACs) in cancer cells. In this report, we demonstrated the mechanism which trichostatin A (TSA), an inhibitor of HDAC, induces expression TbetaRII human pancreatic cell lines modulating transcriptional components bind specific DNA region promoter. This promoter possesses Sp1 and NF-Y binding sites close proximity (located at -102 -83,...
Abstract The role of Smad4 in transforming growth factor β (TGFβ)–mediated epithelial-mesenchymal transition (EMT), invasion, and metastasis was investigated using isogenically matched pancreatic cancer cell lines that differed only expression Smad4. Cells expressing showed an enhanced TGFβ-mediated EMT as determined by increased vimentin decreased β-catenin E-cadherin. invasion suppressed Smad4-intact cells vitro assays, these a reduced orthotopic model cancer. Interestingly, TGFβ inhibited...
Cellular metabolic reprogramming is the main characteristic of cancer cells and identification targets using this pattern extremely important to treat cancers, such as osteosarcoma (OS). In study, SLIT2 ROBO1 were upregulated in OS, higher expression was associated with worse overall survival rate. Furthermore, vitro vivo experiments demonstrated that SLIT2/ROBO1 axis promotes proliferation, inhibits apoptosis, contributes Warburg effect OS cells. Mechanistically, exerted cancer-promoting...
Metastasis significantly reduces the survival rate of osteosarcoma (OS) patients. Therefore, identification novel targets remains extremely important to prevent metastasis and treat OS. In this report, we show that SPARCL1 is downregulated in OS by epigenetic methylation promoter DNA. vitro vivo experiments revealed inhibits metastasis. We further demonstrated SPARCL1-activated WNT/β-catenin signaling physical interaction with various frizzled receptors lipoprotein receptor-related protein...
Abstract Background A sustained inflammatory response following spinal cord injury (SCI) contributes to neuronal damage, inhibiting functional recovery. Macrophages, the major participants in response, transform into foamy macrophages after phagocytosing myelin debris, subsequently releasing factors and amplifying secondary injury. Here, we assessed effect of macrophage scavenger receptor 1 (MSR1) phagocytosis debris SCI explained its possible mechanism. Methods The model was employed...
Aim: Aerobic glycolysis is characteristic of breast cancer. Comprehensive expression profiles key proteins, their prognosis and detailed relationships between miRNAs mRNAs remain unclear. Materials & methods: Oncomine database, Kaplan-Meier overall survival miRNA-mRNA network analysis were performed. A miRNA was identified explored in vitro vivo. Results conclusion: Eleven glycolytic proteins found with higher poor prognosis: GLUT1, SLC2A5, HK1, PFKP, ALDOA, TPI1, GAPDH, PGK1, ENO1, GOT1...
Accumulating evidence indicates that aberrant microRNA (miRNA) expression contributes to osteosarcoma progression. This study aimed elucidate the association between miR-624-5p and (OS) development investigate its underlying mechanism. We analyzed GSE65071 from GEO database found was most upregulated miRNA. The of specific target gene were determined in human OS specimens cell lines by RT-PCR western blot. effects depletion or ectopic on proliferation, migration invasion evaluated vitro...
Spinal cord ischemia-reperfusion (IR) injury (SCIRI) is a significant secondary that causes damage to spinal neurons, leading the impairment of sensory and motor functions. Excessive reactive oxygen species (ROS) production considered one critical mechanism neuron in SCIRI. Nonetheless, molecular mechanisms underlying resistance neurons ROS remain elusive. Our study revealed deletion Git1 mice led poor recovery function after Furthermore, we discovered has beneficial effect on production....
ABSTRACT Problem Endometriosis (EM) is known as a common estrogen‐dependent chronic inflammatory disease. Elevated levels of Forkhead box L2 (FOXL2) have been observed in uterine diseases, including EM. However, the molecular mechanism FOXL2 EM needs to be further illustrated. This study aimed investigate regulatory role rats and isolated ectopic endometrial stromal cells (EC‐ESCs). Method Study knockdown were designed evaluate effects model EC‐ESCs. Hematoxylin‐eosin (HE) staining was used...