A5100639154- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Bacteriophages and microbial interactions
- Hepatitis B Virus Studies
- HIV, Drug Use, Sexual Risk
- Animal Virus Infections Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Hepatitis C virus research
- Sex work and related issues
- vaccines and immunoinformatics approaches
- Viral gastroenteritis research and epidemiology
- Blood groups and transfusion
- Virology and Viral Diseases
- Amino Acid Enzymes and Metabolism
- Identification and Quantification in Food
- Medicinal Plant Research
- Liver Disease Diagnosis and Treatment
- HIV-related health complications and treatments
- Cytomegalovirus and herpesvirus research
- Influenza Virus Research Studies
Beijing Center for Disease Prevention and Control
2014-2024
Center for Disease Control
2024
National Center for AIDS/STD Control and Prevention,China CDC
2021
National Institute for Viral Disease Control and Prevention
2010-2014
Beijing University of Technology
2013-2014
National Institute for Communicable Disease Control and Prevention
2005-2006
Guangzhou Center for Disease Control and Prevention
2006
Chinese Center For Disease Control and Prevention
2005-2006
University of Hong Kong
2006
Abstract Epidemiologic investigations showed that 2 of 4 patients with severe acute respiratory syndrome (SARS) identified in the winter 2003–2004 were a waitress at restaurant Guangzhou, China, served palm civets as food and customer who ate short distance from animal cages. All 6 positive for SARS-associated coronavirus (SARS-CoV). Partial spike (S) gene sequences SARS-CoV identical to 5 S viral civets. Phylogenetic analysis was most closely related isolates. SARS cases result recent...
ABSTRACT Massive numbers of palm civets were culled to remove sources for the reemergence severe acute respiratory syndrome (SARS) in Guangdong Province, China, January 2004, following SARS coronavirus detection market animals. The virus was identified all 91 and 15 raccoon dogs animal origin sampled prior culling, but not 1,107 later at 25 farms, spread over 12 provinces, which claimed be source traded Twenty-seven novel signature variation residues (SNVs) on spike gene analyzed their...
Abstract Human immunodeficiency virus (HIV) sexual transmission among men who have sex with (MSM) has increased markedly in Beijing, China the past decade. Pre-exposure prophylaxis (PrEP) is a highly efficacious biomedical prevention strategy that remarkably reduces HIV-transmission risk. This study examined PrEP awareness MSM and factors influencing it. From April to July 2021, respondent-driven sampling was used conduct cross-sectional survey China. Demographic, behavior, data regarding...
HIV subtypes convey important epidemiological information and possibly influence the rate of disease progression. In this study, progression in patients infected with CRF01_AE, CRF07_BC, subtype B was compared largest molecular epidemiology study ever done China. A national data set pol sequences assembled by pooling from public databases Beijing laboratory network. Logistic regression used to assess factors associated risk AIDS at diagnosis ([AIDSAD], defined as a CD4 count < 200 cells/µL)...
To explore the epidemic history of HIV-1 CRF01_AE in China, 408 fragments gag gene sequences sampled 2002–2010 were determined from different geographical regions and risk populations China. Phylogenetic analysis indicates that can be grouped into four clusters, suggesting at least genetically independent descendants are circulating which two closely related to isolates Thailand Vietnam. Cluster 1 has most extensive distribution In North cluster 4 mainly transmitted through homosexuality.The...
Objectives: Fifteen years after the roll-out of antiretroviral treatment (ART) in China, there is limited information available on transmitted HIV drug resistance (TDR). This study aimed to characterize epidemiology TDR China. Design: We conducted a prospective cross-sectional observational study. Methods: analyzed demographic, clinical, and virological data individuals with newly diagnosed infection using from Beijing laboratory network collected between 2001 2017. did population-based...
Abstract Background National treatment guidelines of China evolving necessitates population-level surveillance transmitted drug resistance (TDR) to inform or update HIV strategies. Methods We analyzed the demographic, clinical, and virologic data obtained from people with (PWH) residing in 31 provinces who were newly diagnosed between 2018 2023. Evidence TDR was defined by World Health Organization list for mutations. Results Among 22 124 PWH protease reverse transcriptase sequences, 965...
Volume 79, no. 18, p. 11892-11900, 2005. Page 11894, Table 1: The GenBank accession numbers of the indicated strains should read as follows: C029G, AY687372; B033G, AY687362; B040G, AY687364; C017G, AY687367; B039G, AY687363; A013G, AY687355; C019G, AY687369; C014G, AY687366; C028G, AY687371; GD03T0013, AY525636; B012G, AY687359; and SZ3, AY304486.
To investigate the prevalence of HIV-1 genotypic mutations for drug resistance among patients in Beijing, blood samples from 145 newly confirmed (2006-2007), treatment-naive HIV-1-infected individuals were analyzed. Seven subtypes or CRF subsequently determined and scored by Stanford HIV Drug Resistance algorithm: CRF01_AE (27.6%), subtype B' (24.1%), CRF07_BC (21.4%), B (20.7%), CRF08_BC (3.4%), C (2.1%), CRF06_cpx (0.7%). Eleven subjects studied found to harbor strains resistant either...
To investigate the origin and evolutionary history of spread HIV-1 subtype B in China, a total 409 sequences pol gene sampled from 1994 to 2012 29 provinces across China was subjected phylogenetic Bayesian molecular clock analyses. The study reveals that strains are genetically diverse can be classified into four distinct subgroups, namely B′ (Thai-B), BJ-B (Beijing-B), Pan-B (Pandemic-B), TW-B (Taiwan-B), according sequences. reported for first time. Phylogeographic analysis exhibits...
Beijing is a national and international hub potentially containing broad diversity of HIV variants. Previous studies on molecular epidemiology in pooled together samples from residents non-residents. Pooling non-residents has introduced bias undermined good assessment the intervention among autochthonous population. Here, we aimed to define subtype investigate TDR exclusively.We analyzed demographic, clinical, virological data collected between 2001 2016 Beijing. A population-based...
The purpose of this study was to investigate the genetic diversity HIV-1 circulating in Beijing and its molecular epidemiological linkages with regard risk factors viral transmission. from plasma samples 280 diagnosed individuals (2006–2007) characterized. gene fragments gag, pol, env infected were amplified by reverse transcriptase polymerase chain reaction (RT-PCR), sequenced, phylogenetically analyzed. From analyzed, a total 496 sequences successfully genes. Nine group M subtypes or CRF...
In China, few molecular epidemiological data on hepatitis C virus (HCV) are available and all previous studies were limited by small sample sizes or specific population characteristics. Here, we report characterization of the epidemic history transmission dynamics HCV strains in China. We included sequences individuals belonging to three surveillance programs: 1) patients diagnosed with HIV infection at Beijing laboratory network, most whom people who inject drugs former paid blood donors,...
Beijing has seen a rising epidemic of HIV among students. However, little information was known about the molecular epidemiologic data HIV-infected In this study, diversity and prevalence transmitted drug resistance (TDR) in pol sequences derived from 237 students were analyzed. TDR mutations found five men who have sex with (MSM) population The overall 2.1%, comprising 1.3% protease inhibitors 0.8% non-nucleoside reverse transcriptase inhibitors. Our finding indicates low-level Beijing.
CRF01_AE is one of the four dominant HIV-1 strains circulating in China. In this study, we performed genetic and phenotypic analyses using a total 60 full-length envelope gene clones from 14 HIV-1-infected individuals Beijing area. Among sequences analyzed, 32 have complete open reading frame (ORF), whereas others contain premature stop codons. The phylogenetic tree analysis suggested that all maintained close relationship with strain. Most potential N-linked glycosylation sites (PNGS) were...
HIV-1 CRF01_AE and CRF07_BC have been two mainly circulating strains in the men who sex with (MSM) population Beijing for years. These subtypes together were accounting 78.1% of positive cases newly diagnosed Beijing, 2016. In this study, we report a novel CRF01_AE/CRF07_BC second-generation unique recombinant form (URF) (named DT1427_NFLG) identified MSM population. The near full-length genome DT1427_NFLG is about 8.8 kb four fragments inserted into backbone. China, several URFs reported...
Little is known about the molecular and biological properties of HIV-1 intersubtype B'/C in Beijing. To fill gap, we sequenced analyzed gag-pol genes from 39 recombinant infectors Beijing, China during 2007. The results show that 36 CRF07_BC 2 CRF08_BC isolates have a structural profile identical or nearly to according sequences regions. circulating injecting drug users (IDUs) heterosexuals forms diverse phylogenetic tree most homosexuals cluster together. However, all strains were...
In China, CRF01_AE and CRF07_BC are the most circulating strains of HIV-1, more second-generation recombinant forms have been isolated around two strains. The same situation happened in Beijing recent years. our study, we a new form HIV-1 from male patient who was infected by homosexual behavior. Polygenetic analysis revealed that sequence includes five fragments four fragments. is skeleton which inserted with emergency such manifests diversity epidemic. Consequently, further investigation...
Abstract Background In 2016, China has implemented the World Health Organization's “treat all” policy. We aimed to assess impact of significant improvements in 95-95-95 targets on population-level human immunodeficiency virus (HIV) transmission dynamics and incidence. Methods focused 3 steps HIV care continuum: diagnosed, antiretroviral therapy, achieving viral suppression. The molecular clusters were inferred using HIV-TRACE. New infections estimated incidence method European Centre for...
Objective To construct recombinant MVA vaccine encoding codon-optimized HIV-1 CRF01_AE gp145 gene and evaluate its immunogenicity in mice. Methods The intracellular region of codon-modified consensus env obtained our preliminary study was removed to get gene. The modified cloned into shuttle plasmid pSC11.BHK-21 cells infected with wild-type advance were transfected the plasmid. Then expressing rMVA-AEgp145 by homologous recombination.After expression Gp145 confirmed WB assay, BALB/c...