A5101493141- Cancer, Hypoxia, and Metabolism
- Bone Metabolism and Diseases
- Bone health and treatments
- Immune Cell Function and Interaction
- Immune cells in cancer
- Biochemical and Molecular Research
- Cytokine Signaling Pathways and Interactions
- Endoplasmic Reticulum Stress and Disease
- T-cell and B-cell Immunology
- vaccines and immunoinformatics approaches
- Phytochemistry and biological activities of Ficus species
- Immunotherapy and Immune Responses
- Bone and Joint Diseases
- Psoriasis: Treatment and Pathogenesis
- Ginkgo biloba and Cashew Applications
- Neurogenetic and Muscular Disorders Research
- PI3K/AKT/mTOR signaling in cancer
- Quinazolinone synthesis and applications
- Autophagy in Disease and Therapy
- IL-33, ST2, and ILC Pathways
- Bone and Dental Protein Studies
- Ginseng Biological Effects and Applications
- Fibroblast Growth Factor Research
- interferon and immune responses
- RNA Interference and Gene Delivery
Universitätsklinikum Erlangen
2018-2024
Friedrich-Alexander-Universität Erlangen-Nürnberg
2016-2024
Shenyang Pharmaceutical University
2023-2024
First Affiliated Hospital of Zhengzhou University
2020
Dalian Medical University
2019-2020
Nankai University
2011-2014
State Key Laboratory of Medicinal Chemical Biology
2012
Wuhan University
2011
Anhui Medical University
2011
University of Science and Technology of China
2011
Hypoxia-inducible factors (HIFs) are key elements for controlling immune cell metabolism and functions. While HIFs known to be involved in T cells macrophages activation, their functions B lymphocytes poorly defined. Here, we show that hypoxia-inducible factor-1α (HIF-1α) contributes IL-10 production by cells. HIF-1α regulates expression, HIF-1α-dependent glycolysis facilitates CD1dhiCD5+ expansion. Mice with cell-specific deletion of Hif1a have reduced number IL-10-producing cells, which...
Abstract Screening of the entire let-7 family microRNAs (miRNA) by in situ hybridization identified let-7g as only member, diminished expression which was significantly associated with lymph node metastasis and poor survival breast cancer patients. Abrogation otherwise nonmetastatic mammary carcinoma cells elicited rapid from orthotopic location, through preferential targets, Grb2-associated binding protein 2 (GAB2) fibronectin 1 (FN1), consequent activation p44/42 mitogen-activated kinase...
To elucidate the function of oxidative phosphorylation (OxPhos) during B cell differentiation, we employ CD23Cre-driven expression dominant-negative K320E mutant mitochondrial helicase Twinkle (DNT). DNT-expression depletes DNA maturation, reduces abundance respiratory chain protein subunits encoded by DNA, and, consequently, super-complexes in activated cells. Whereas development DNT mice is normal, proliferation, germinal centers, class switch to IgG, plasma and T cell-dependent as well...
Objectives To investigate the effect of L-arginine metabolism on arthritis and inflammation-mediated bone loss. Methods was applied to three models (collagen-induced arthritis, serum-induced human TNF transgenic mice). Inflammation assessed clinically histologically, while changes were quantified by μCT histomorphometry. In vitro, effects osteoclast differentiation analysed RNA-seq mass spectrometry (MS). Seahorse, Single Cell ENergetIc profilIng Translation inHibition transmission electron...
Metabolic changes are crucially involved in osteoclast development and may contribute to bone degradation rheumatoid arthritis (RA). The enzyme aconitate decarboxylase 1 (Acod1) is known link the cellular function of monocyte-derived macrophages their metabolic status. As osteoclasts derive from monocyte lineage, we hypothesised a role for Acod1 its metabolite itaconate differentiation arthritis-associated loss.
Methamphetamine (METH) leads to nervous system toxicity. Long-term exposure METH results in damage dopamine neurons the ventral tegmental area (VTA), and depression-like behavior is a clinical symptom of this The current study was designed investigate whether antioxidant tertiary butylhydroquinone (TBHQ) can alleviate neurotoxicity through both antioxidative stress antiapoptotic signaling pathways VTA. Rats were randomly divided into control group, METH-treated group (METH group),...
In the bone marrow, B cells and bone-resorbing osteoclasts colocalize form a specific microenvironment. How functionally influence architecture is poorly understood. Using genetically modified mice high-throughput analyses, we demonstrate that prolonged HIF-1α signaling in leads to enhanced RANKL production osteoclast formation. addition, deletion of prevents estrogen deficiency-induced loss mice. Mechanistically, controls protein stabilization through HSP70-mediated degradation marrow...
Although it is known that psoriasis strongly associated with obesity, the mechanistic connection between diet and skin lesions not well established. Herein, we showed only dietary fat, carbohydrates or proteins, exacerbates psoriatic disease. Enhanced inflammation was changes in intestinal mucus layer microbiota composition by high-fat (HFD). Change of vancomycin treatment effectively blocked activation HFD, inhibited systemic interleukin-17 (IL-17) response, led to increased mucophilic...
The impact of metabolic stress induced by obesity on the bone marrow melanoma niche is largely unknown. Here we employed diet obese mice model, where received high-fat (HFD) or normal (ND) for 6 weeks before challenge with B16F10 cells. Tumor size, loss and osteoclasts numbers were assessed histologically in tibial bones. For defining molecular pathway, osteopontin knock-out mice, interleukin neutralizing antibody Janus kinase 2 inhibition carried out same model. Mechanistic studies such as...
Coactivators are a heterogeneous family of transcriptional regulators that essential for modulation outcomes and fine-tune numerous cellular processes. The aim the present study was to evaluate role coactivator peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) in pathogenesis systemic sclerosis (SSc).Expression PGC-1α analysed by real-time PCR, western blot immunofluorescence. Modulation autophagy reporter studies expression autophagy-related genes. effects knockdown...
Background Human malignant melanoma is a highly aggressive, heterogeneous and drug-resistant cancer. Due to high number of clones, harboring various mutations that affect key pathways, there an exceptional level phenotypic variation intratumor heterogeneity (ITH) in melanoma. This poses significant challenge personalized cancer medicine. Hitherto, it remains unclear what extent the affects immune microenvironment. Herein, we explore interaction between tumor host response cohort utilizing...
Abstract Germinal centers where B cells undergo clonal expansion and antibody affinity maturation are hypoxic microenvironments. However, the function of hypoxia-inducible factor (HIF)-1α in immunoglobulin production remains incompletely characterized. Here, we demonstrated that lacking HIF-1α exhibited significantly lower glycolytic metabolism impaired IgA production. Loss affects IgA-producing B-cell differentiation exacerbates dextran sodium sulfate (DSS)-induced colitis. Conversely,...
<h2>Summary</h2><h3>Background</h3> Hand hygiene is a critical strategy for infection prevention in all healthcare settings. Automated electronic monitoring systems are expected to improve hand performance. <h3>Aim</h3> To investigate the impact of Internet Things (IoT) management system on compliance among medical staff an emergency intensive care unit (EICU). <h3>Methods</h3> This retrospective observational study was conducted between July 1<sup>st</sup>, 2017 and February...
We have previously demonstrated that DCB-3503, a tylophorine analogue, has an anti-inflammatory property in murine models for autoimmune diseases. However, its mechanism remains unknown. Here, we synthesized 34 derivatives of DCB-3503 and investigated their effects on T cells differentiation TNF-α production. Six (4, 9, 13, 19, 31, 32) could significantly promote the expression Foxp3. Among these, IC50 31 32 was about 500 μM. Eight analogues (1, 2, 4, 12, 18, 21) showed anti-TNF-α effect Raw...
Alzheimer’s disease (AD) is a common neurodegenerative disease. Abundant evidence demonstrates that oxidative stress may be not only an early event in this disease, but also key factor the pathogenesis of AD. Ginkgo biloba extract (EGb) has strong ability to scavenge oxygen free radicals and supply hydrogen. The present study aims investigate effects EGb on Neuro 2A cells transfected with Swedish mutant APP (APPsw). Stably cell lines expressing human wild-type (APP695), APPsw, or empty...
To investigate how the mucosal barrier in intestine influences development of arthritis, considering that metabolic changes intestinal epithelium influence its function.
Abstract A novel tylophorine analog W-8 enhanced Foxp3 expression through both transcriptional and epigenetic programs, its ameliorated TNBS induced colitis. Tylophorine analogs are phenanthroindolizidine alkaloids, several of which have been reported to anticancer, antiviral, anti-inflammatory properties. However, their function in the immune system remains widely unknown. Transcription factor is critical for development Treg, down-regulates maintains tolerance self-antigens. In present...
Although many cancer vaccines have been developed against type I MAGE (melanoma antigen) genes owing to their shared tumour-specific expression properties, studies about and functions are relatively limited. In the present study, we first identify a non-testis-specific gene, Mageb18 antigen family B 18). Mouse is also expressed in digestion- immune-related tissues as well testis, its testis age-dependent. mouse-derived cell lines, DNA demethylation histone acetylation mediate reactivation of...
AD-2 (20(R)-dammarane-3β, 12β, 20, 25-tetrol, 25-OH-PPD) was structurally modified to introduce additional amino groups, which can better exert its anti-tumor effects in MCF-7, A549, LoVo, HCT-116, HT -29, and U-87 cell lines. We investigated the cellular activity of 15 different acid derivatives on HepG2 cells possible mechanism action superior derivative 6b. An MTT assay used detect cytotoxicity derivatives. Western blotting study signaling pathways. Flow cytometry apoptosis ghost pen...
Abstract The function of mitochondrial respiration during B cell fate decisions and differentiation remains equivocal. This study reveals that selection for fitness occurs activation is essential subsequent plasma differentiation. By expressing a mutated helicase in transitional cells, we depleted DNA maturation, resulting reduced oxidative phosphorylation. Although no changes follicular development were evident, germinal centers, class switch recombination to IgG, generation humoral...