A5102820379- Clostridium difficile and Clostridium perfringens research
- Antibiotic Resistance in Bacteria
- Gut microbiota and health
- Fungal Infections and Studies
- Microscopic Colitis
- Viral gastroenteritis research and epidemiology
- Antifungal resistance and susceptibility
- SARS-CoV-2 and COVID-19 Research
- Vibrio bacteria research studies
- Helicobacter pylori-related gastroenterology studies
- Bacterial Identification and Susceptibility Testing
- COVID-19 Clinical Research Studies
- Bacterial biofilms and quorum sensing
- Medical Research and Treatments
- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- Respiratory viral infections research
- Burkholderia infections and melioidosis
- Pharmacological Effects of Natural Compounds
- Nosocomial Infections in ICU
- Bacteriophages and microbial interactions
- Mass Spectrometry Techniques and Applications
- Pharmaceutical and Antibiotic Environmental Impacts
- Escherichia coli research studies
- Streptococcal Infections and Treatments
National Institute for Communicable Disease Control and Prevention
2015-2024
Xuzhou Medical College
2021
State Key Laboratory of Infectious Disease Prevention and Control
2011-2021
Chinese Center For Disease Control and Prevention
2013-2020
National Health Laboratory Service
2019
Engineering Associates (United States)
2017
Soochow University
2011-2013
First Affiliated Hospital of Soochow University
2011-2013
Vaccine candidate tested in monkeys Global spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to an urgent race develop a vaccine. Gao et al. report preclinical results early vaccine called PiCoVacc, which protected rhesus macaque against SARS-CoV-2 infection when analyzed short-term studies. The researchers obtained multiple strains from 11 hospitalized patients across the world and then chemically inactivated harmful properties virus. Animals were immunized with...
ABSTRACT BACKGROUND The top priority for the control of COVID-19 pandemic currently is development a vaccine. A phase 2 trial conducted to further evaluate immunogenicity and safety SARS-CoV-2 inactivated vaccine (CoronaVac). METHODS We randomized, double-blind, placebo-controlled optimal dose, CoronaVac. total 600 healthy adults aged 18-59 years were randomly assigned receive injections at dose 3 μg/0.5 mL or 6 μg /0.5mL, placebo on Day 0,14 schedule 0,28 schedule. For evaluation, solicited...
Abstract The COVID-19 pandemic caused by SARS-CoV-2 has brought about an unprecedented crisis, taking a heavy toll on human health, lives as well the global economy. There are no SARS-CoV-2-specific treatments or vaccines available due to novelty of this virus. Hence, rapid development effective against is urgently needed. Here we developed pilot-scale production purified inactivated virus vaccine candidate (PiCoVacc), which induced neutralizing antibodies in mice, rats and non-human...
Typically dealing with practical samples very complex matrices, ambient ionization mass spectrometry suffers from low detection sensitivity. In this study, molecular imprinting technology was explored and integrated the membrane electrospray (MESI) method for direct sample analyses. By enriching targeted analytes on molecularly imprinted membranes (MIMs), improvement (by 10- to 50-fold) in limit of quantitation could be achieved, compared conventional nanoelectrospray methods or other...
SUMMARY The phylogenetic and epidemic relationships of 104 clinical isolates Clostridium difficile from three hospitals different geographical population sources in China were investigated by multilocus sequence typing. Twenty-two types (STs) identified, four which, ST117, ST118, ST119 ST129, novel. No geographically specific host population-specific lineages found there was no correlation between origin or strain genotype. ST37 the dominant type our survey but novel STs underline high...
Clostridium difficile infection (CDI) has become a worldwide public health problem causing high mortality and large disease burden. Molecular typing analysis is key important for surveillance control of CDI. However, molecular characterization C. across China extremely rare. To investigate the multicenter data isolates China, toxin profile, types, epidemiological characteristics were analyzed. A total 199 collected from 2010-2015 analyzed using multilocus sequence (MLST), PCR ribotyping,...
Clostridium difficile is a well-known nosocomial infectious pathogen. Research on C. infection has primarily focused strains such as the hypervirulent PCR ribotype 027 (sequence type 1 [ST1]) emerging in Europe and North America. However, other new ribotypes some countries have attracted attention, 17 (ST37) Asia Latin We collected 70 sequenced their toxin genes, tcdA tcdB. Multilocus sequence typing (MLST) was used to study population structure. In addition, and/or tcdB sequences of 25...
The nucleotide sequences of five plasmids from one Klebsiella oxytoca isolate were determined using the PacBio RS II system. Plasmid analysis revealed that blaNDM-1 was carried on an IncX3 plasmid. blaIMP-4 and blaKPC-2 genes located IncN IncP-6 plasmids, respectively. Comparative sequence highlighted successful spread carbapenemase-harboring among different enterobacterial species. We report for first time, to our knowledge, coproducing NDM-1, KPC-2, IMP-4 carbapenemases a K. isolate.
Candida tropicalis is considered to be the leading pathogen causing nosocomial fungemia and hepatosplenic fungal infections in patients with cancer, particularly those leukemia. Microsatellite-based typing methods using sets of genetic markers have been developed reported for population structure analysis C. albicans, glabrata, parapsilosis, but no studies published tropicalis. The objective this study was develop new microsatellite loci that ability distinguish among isolates. DNA sequences...
The nasopharyngeal microbiota is composed of a variety not only the true commensal bacterial species but also two-face pathobionts, which are one harmless and other highly invasive deadly pathogen. In previous study, we found that diversity was lost in severe influenza patients.
Candia tropicalis is an increasingly important human pathogen, causing nosocomial fungemia among patients with neutropenia or malignancy (Guinea, 2014). However, limited research has been published concerning its pathogenicity. Based on the phenotypes of C. in our previous study, we selected nine representative strains different activities virulence factors (adhesion, biofilm formation, secreted aspartic proteinases and hemolysins), one reference strain, ATCC750. The present study aimed to...
Melioidosis is a severe infectious disease caused by gram-negative, facultative intracellular pathogen Burkholderia pseudomallei (B. pseudomallei). Although cases are increasing reported from other parts of the world, it an illness tropical and subtropical climates primarily found in southeast Asia northern Australia. Because 40% mortality rate, this life-threatening poses public health risk endemic area. Early detection B. infection vital for prognosis melioidosis patient. In study, novel...
Escherichia coli sequence type 131 (ST131) is well known for its multidrug resistance profile. Carbapenems have been considered the treatment of choice E. ST131 infections, and to carbapenems emerging due acquisition carbapenemase-encoding genes. In this study, 45 carbapenem-resistant strains were collected in a hospital. The mechanisms, plasmid profiles, genetic relatedness these determined. Phylogenetic relationships between assessed by molecular profiling aligned with patient clinical...
The objective of this study was to construct a novel strategy for the detection severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants using multiplex PCR-mass spectrometry minisequencing technique (mPCR-MS minisequencing). Using nucleic acid sequence SARS-CoV-2 nonvariant and synthetic variant-carrying plasmid, matrix-assisted laser desorption ionization-time flight mass (MALDI-TOF MS) method based on single-base probe extension PCR amplification products established detect 9...
An outbreak of coronavirus disease 2019 (COVID-19) Omicron BA.2 emerged in Jilin City since March 3, 2022, which involved 27,036 cases by April 12. The vaccination program with inactivated COVID-19 vaccines has been implemented the beginning 2021.The incidences moderate, severe, and critical whole population group 0+1 dose were 1.82-, 9.49-, 3.85-fold higher than those 2 doses, 5.03-, 44.47-, ∞-fold received 3 doses vaccination. For ≥60 years, 29.92, 9.62, 4.27 per 100,000, showing 4.13-,...
Abstract Background Invasive fungal infections caused by uncommon fungi have increased in recent years. Hospitalized low-birth-weight infants are at high risk for neonatal infections. Pichia fabianii is a rare pathogen causing blood infection, which has reportedly only 4 cases of fungemia and 1 case endocarditis worldwide. Here, we describe the first P . infection premature infant China. Case presentation On July 28 th , (LBW, 1760 g) female born 33 +4 weeks gestation was admitted to...
Abstract Background It has been performed worldwidely to explore the potential of animals that might be a reservoir for community associated human infections Clostridioides difficile . Several genetically undistinguished PCR ribotypes C. from and have reported, illustrating transmission between them. Pig calf were considered as main origins with predominant RT078 RT033, respectively. As more investigations involved, great diversity molecular types pig reported in Europe, North American...
This study developed a new single-tube multiplex real-time PCR method for detecting toxigenic C. difficile directly from fecal samples using tcdA, tcdB, cdtB, and internal gene tpi as targets, which could be performed on kinds of polymerase chain reaction device including point-of-care testing (POCT), with improved detection efficiency. The specificity, sensitivity, repeatability each was evaluated 69 isolates 74 samples. Results were compared established PCR, qPCR, ELISA methods....
// Min Yuan 1 , Hai Chen 2 Xiong Zhu Jiao Feng 3 Zhe Zhan Defu Zhang Xia Xiaofei Zhao Jinxing Lu Jianguo Xu Dongsheng Zhou and Juan Li State Key Laboratory of Infectious Diseases Prevention Control, Collaborative Innovation Center for Diagnosis Treatment Disease, National Institute Communicable Disease Control Prevention, Chinese Beijing 102206, China Department Clinical Laboratory, People's Hospital Sanya, Hainan 572000, Pathogen Biosecurity, Microbiology Epidemiology, 100071,...
Mobile genetic elements play a key role in the continuing evolution of Clostridium difficile , resulting emergence new phenotypes for individual isolates. On basis whole-genome sequencing analysis, we comprehensively explored transposons, CRISPR, prophage, and sites drug resistance within clade 4 C. isolates with different sequence types. Great diversity MGEs high rate multidrug were found this clade, including Tn 4453a/b aac(6 ′ ) aph(2 ′′ instead catD relatively prophage-carried CRISPR...