A5073750380- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Metabolism and Genetic Disorders
- Gut microbiota and health
- Advanced NMR Techniques and Applications
- Immune cells in cancer
- Lipid metabolism and biosynthesis
- Advanced MRI Techniques and Applications
- Air Quality and Health Impacts
- Cytomegalovirus and herpesvirus research
- Food composition and properties
- Adenosine and Purinergic Signaling
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Structural Load-Bearing Analysis
- RNA Research and Splicing
- Microbial Metabolites in Food Biotechnology
- Signaling Pathways in Disease
- GABA and Rice Research
- Lysosomal Storage Disorders Research
- Glycogen Storage Diseases and Myoclonus
- Lung Cancer Treatments and Mutations
- Climate Change and Health Impacts
Zhejiang Cancer Hospital
2025
Chinese Academy of Sciences
2025
BGI Group (China)
2025
First Affiliated Hospital of Guangzhou University of Chinese Medicine
2025
University of Kentucky
2017-2024
Markey Cancer Center
2017-2024
Sun Yat-sen University
2017-2022
Guangxi Yuchai Machinery Group (China)
2021
Guilin Medical University
2021
Hospital de Sant Pau
2018
More than 100 distinct chemical modifications to RNA have been characterized so far.However, the prevalence, mechanisms and functions of various remain largely unknown.To provide transcriptome-wide landscapes modifications, we developed RMBase v2.0 (http:// rna.sysu.edu.cn/rmbase/), which is a comprehensive database that integrates epitranscriptome sequencing data for exploration post-transcriptional RNAs their relationships with miRNA binding events, disease-related singlenucleotide...
T cells undergo metabolic rewiring to meet their bioenergetic, biosynthetic and redox demands following antigen stimulation. To fulfil these needs, effector must adapt fluctuations in environmental nutrient levels at sites of infection inflammation. Here, we show that can utilize inosine, as an alternative substrate, support cell growth function the absence glucose vitro. metabolize inosine into hypoxanthine phosphorylated ribose by purine nucleoside phosphorylase. We demonstrate subunit...
Abstract Dissecting the spatial heterogeneity of cancer-associated fibroblasts (CAFs) is vital for understanding tumor biology and therapeutic design. By combining pathological image analysis with proteomics, we revealed two stromal archetypes in hepatocellular carcinoma (HCC) different biological functions extracellular matrix compositions. Using paired single-cell RNA epigenomic sequencing Stereo-seq, fibroblast subsets CAF-FAP CAF-C7, whose enrichment strongly correlated opposing patient...
Abstract Exposure to ambient air particulate matter (PM 2.5 ) is well established as a risk factor for cardiovascular and pulmonary disease. Both epidemiologic controlled exposure studies in humans animals have demonstrated an association between pollution metabolic disorders such diabetes. Given the central role of liver peripheral glucose homeostasis, we exposed mice filtered or PM 16 weeks examined its effect on hepatic pathways using stable isotope resolved metabolomics (SIRM) following...
Although T cell expansion depends on glycolysis, effector differentiation requires signaling via the production of reactive oxygen species (ROS). Because pentose phosphate pathway (PPP) regulates ROS by generating nicotinamide adenine dinucleotide (NADPH), we examined how PPP blockade affects and function. Here, show that genetic ablation or pharmacologic inhibition enzyme 6-phosphogluconate dehydrogenase (6PGD) in oxidative results generation superior CD8
γ-Aminobutyrate (GAB), the biochemical form of (GABA) γ-aminobutyric acid, participates in shaping physiological processes, including immune response. How GAB metabolism is controlled to mediate such functions remains elusive. Here we show that one most abundant metabolites CD4
Metabolic differences among and within tumors can be an important determinant in cancer treatment outcome. However, methods for determining these non-invasively vivo is lacking. Using pancreatic ductal adenocarcinoma as a model, we demonstrate that tumor xenografts with similar genetic background distinguished by their differing rates of the metabolism 13C labeled glucose tracers, which imaged without hyperpolarization using newly developed techniques noise suppression. this method, subtypes...
Polypeptides encoded by long noncoding RNAs (lncRNAs) are a novel class of functional molecules. However, whether these hidden polypeptides participate in the TP53 pathway and play significant biological role is still unclear. Here, we discover that TP53-regulated lncRNAs can encode peptides, two which various human cell lines. Using ribosome profiling RNA-seq approaches HepG2 cells, systematically identified more than 300 further confirmed 15 peptides. Furthermore, several peptides were...
Objective The goal of the study was to identify potential nutrigenetic effects inulin, a prebiotic fiber, in mice with different human apolipoprotein E (APOE) genetic variants. Specifically, we compared responses inulin for modulation systemic metabolism and neuroprotection via gut-brain axis APOE ϵ3 ϵ4 alleles.Method We performed experiments young expressing APOE3 (E3FAD APOE4 gene (E4FAD mice). fed either or control diet 16 weeks starting from 3 months age. determined gut microbiome...
Lipids comprise diverse classes of compounds that are important for the structure and properties membranes, as high-energy fuel sources signaling molecules. Therefore, turnover rates these varied lipids fundamental to cellular function. However, their enormous chemical diversity dynamic range in cells makes detailed analysis very complex. Furthermore, although stable isotope tracers enable determination synthesis degradation complex lipids, numbers distinguishable molecules increase...
Immunomodulatory (IM) metabolic reprogramming in macrophages (Mϕs) is fundamental to immune function. However, limited information available for human Mϕs, particularly response plasticity, which critical understanding the variable efficacy of immunotherapies cancer patients. We carried out an in-depth analysis by combining multiplex stable isotope-resolved metabolomics with reversed phase protein array map dynamic changes IM network and key regulators four donors' Mϕs differential...
Biologically important 2-hydroxy carboxylates such as lactate, malate, and 2-hydroxyglutarate exist in two enantiomeric forms that cannot be distinguished under achiral conditions. The D L (or R, S) enantiomers have different biological origins functions, therefore, there is a need for simple method resolving, identifying, quantifying these enantiomers. We adapted improved chiral derivatization technique nuclear magnetic resonance (NMR), which needs no chromatography enantiomer resolution,...
EZH2 (Enhancer of Zeste Homolog 2), a subunit Polycomb Repressive Complex 2 (PRC2), catalyzes the trimethylation histone H3 at lysine 27 (H3K27me3), which represses expression genes. It also has PRC2-independent functions, including transcriptional coactivation oncogenes, and is frequently overexpressed in lung cancers. Clinically, inhibition can be achieved with FDA-approved drug EPZ-6438 (tazemetostat). To realize full potential blockade, it critical to understand how cell-cell/cell-matrix...
A substantial fraction of common metabolites contains carboxyl functional groups. Their 13C isotopomer analysis by nuclear magnetic resonance (NMR) is hampered the low sensitivity nucleus, slow longitudinal relaxation for lack an attached proton, and relatively chemical shift dispersion carboxylates. Chemoselective (CS) derivatization a means tagging compounds in complex mixture via specific group. 15N1-cholamine has been shown to be useful CS agent carboxylates, producing peptide bond that...