A5013227008- Ferroptosis and cancer prognosis
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- Thyroid Cancer Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- Cancer Genomics and Diagnostics
- Bladder and Urothelial Cancer Treatments
- Lung Cancer Treatments and Mutations
- Glycosylation and Glycoproteins Research
- Hepatocellular Carcinoma Treatment and Prognosis
- Advanced Proteomics Techniques and Applications
- Hydrogen embrittlement and corrosion behaviors in metals
- Protease and Inhibitor Mechanisms
- High Temperature Alloys and Creep
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Microtubule and mitosis dynamics
- Fatigue and fracture mechanics
- Heat shock proteins research
- Circular RNAs in diseases
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2025
Nankai University
2019-2025
China West Normal University
2024
Zhongnan Hospital of Wuhan University
2024
Wuhan University
2024
Zhejiang University
2023-2024
China General Nuclear Power Corporation (China)
2023-2024
University of Science and Technology
2024
National Center for Nanoscience and Technology
2023
Peking Union Medical College Hospital
2013-2022
Background Neutrophil extracellular traps (NETs) were originally thought to be formed by neutrophils trap invading microorganisms as a defense mechanism. Increasing studies have shown that NETs play pivotal role in tumor progression and diffusion. In this case, transcriptome analysis provides an opportunity unearth the association between clinical outcomes of patients with pan-cancer. Methods The sequencing data Cancer Genome Atlas pan-cancer primary focus was obtained from UCSC Xena,...
The extracellular matrix (ECM) molecule osteopontin is implicated in many pathologic processes, including inflammation, cell proliferation, ECM invasion, tumor progression, and metastasis. present study evaluated the clinical biological importance of human lung cancer.Tissue microarrays derived from non-small cancer (NSCLC) patients were analyzed immunohistochemically. Osteopontin protein expression was observed 64.5% (205 318) primary tumors 75.5% (108 143) lymph node metastases, but only...
Early stage lung cancer detection is the first step toward successful clinical therapy and increased patient survival. Clinicians monitor progression by profiling tumor cell proteins in blood plasma of afflicted patients. Blood plasma, however, a difficult protein assessment medium because it rich albumins heterogeneous species. We report herein method to detect released into circulatory system cells. Initially we analyzed components conditioned (CM) primary or organ cultures adjacent normal...
The transmembrane and secreted protein delta-like 1 homolog (DLK1) belongs to the EGF-like family. It is widely accepted that DLK1 plays important roles in regulating cell differentiation, such as adipogenesis osteogenesis. Aberrant expression of has been found various types human cancers, including lung cancer. A previous study this lab revealed associated with tumor invasion, although mechanism still unknown. To explore potential effects might have on was overexpressed or knocked down...
844 Background: Urothelial carcinoma (UC) remains one of the most common urologic cancers, presenting challenges in both initial diagnosis and follow-up due to invasive nature traditional diagnostic procedures. Urinary cell-free DNA (ucfDNA) analysis offers a non-invasive alternative, with methylation patterns providing promising avenue for identifying tumor-derived cfDNA. This study explores utility ucfDNA methylation-based tumor fraction (mTF) early detection monitoring recurrence UC...
846 Background: Traditional diagnostic procedures for urothelial carcinoma (UC) are often limited by patient burden and reduced sensitivity, impacting accuracy. Liquid biopsy-based molecular detection, particularly through fragmentomics analysis, offers a promising supplement to clinical applications. Fragmentomics, which examines the unique fragmentation patterns of tumor DNA, can provide crucial insights into origin behavior. This study aims leverage urinary cell-free DNA (ucfDNA) enhance...
Despite advancements in immunotherapy, particularly regarding programmed cell death protein 1 (PD‑1)/programmed death‑ligand blockades, the clinical outcomes non‑small lung cancer (NSCLC) remain variable with limited predictive biomarkers currently available. The present study investigated respiratory microbiota diversity as a potential biomarker to predict efficacy of PD‑1 blockades patients advanced NSCLC. A retrospective analysis was conducted on 60 treated from May 2019 2023. Clinical...
Abstract PURPOSE: Expression of targeting protein for Xklp2 (TPX2), a microtubule-associated protein, is tightly cell cycle regulated. Abnormally expressed TPX2 has been reported in various malignancies, but less known lung cancer. The present study appraised the significance aberrant expression tumorigenesis and progression human squamous carcinoma (SCC) lung. EXPERIMENTAL DESIGN AND RESULTS: expressive status was firstly examined with cancer (L, PAa, PG) immortalized bronchial epithelial...
<h2>Abstract</h2> As sorafenib is a first-line drug for treating advanced hepatocellular carcinoma, resistance has historically attracted attention. However, most of this attention been focused on series mechanisms related to arising after treatment. In study, we used proteomic techniques explore the potential by which pretreatment factors affect resistance. The degree redundant pathway PI3K/AKT activation, biotransformation capacity, and autophagy level in carcinoma patients prior treatment...
The early diagnosis of lung cancer is an effective approach to reduce the mortality caused by malignancy. To explore serum biomarkers at stage, M-BE, a SV40T-transformed human bronchial epithelial cell line with phenotypic features tumorigenesis high passage, was cultured in conditioned media collect its secretory proteins. proteins secreted from different passage M-BE cells were extracted and then separated two-dimensional electrophoresis (2-DE). MALDI-TOF/TOF mass spectrometry adopted...
The purpose of the present investigation is to determine whether centrosome amplifications are in breast tumor cells, there differences amplification between benign lesions and carcinomas, centrosomal analysis can be value diagnosis prognosis carcinoma.Using immunofluorescence with an antibody against gamma-tubulin, we analyzed abnormalities fine-needle aspirations 100 (25 cases 75 carcinomas).We found that amplifications, including numerical structural amplification, were most tumors. Cells...
Abstract Background As an indicator of tumor invasiveness, microvascular invasion (MVI) is a crucial risk factor for postoperative relapse, metastasis, and unfavorable prognosis in hepatocellular carcinoma (HCC). Nevertheless, the genetic mechanisms underlying MVI, particularly Chinese patients, remain mostly uncharted. Methods We applied deep targeted sequencing on 66 HCC samples. Focusing telomerase reverse transcriptase (TERT) promoter (TERTp) TP53 co‐mutation (TERTp+/TP53+) group, gene...
Hypothetically, intratumoral genomic heterogeneity has the potential to foster tumor-infiltrating lymphocyte (TIL) diversity; however, no study directly tested this hypothesis by simultaneously investigating somatic mutations, TIL diversity, and immune response activity. Thus, we performed whole-exome sequencing, repertoire sequencing gene expression on ten spatially separated tumor samples obtained from two masses excised a glioblastoma multiforme (GBM) patient, included peripheral blood as...
Breast cancer is a heterogeneous disease, and although advances in molecular subtyping have been achieved recent years, most strategies target individual genes independent of one another primarily concentrate on proliferative markers. The contributions biological processes immune patterns neglected breast subtype stratification.We performed gene set variation analysis to simplify the information using hallmark terms decompose cell data 985 invasive ductal/lobular carcinoma RNAseq samples...
Circulating tumour cell (CTC) behaviours are distinct from those of bulk tissues. Thus, treatments to eliminate CTCs differ the regimens followed reduce primary and its metastases. Accordingly, comprehensively deciphering single nucleotide variant (SNV) profiles in CTCs, which partially determine CTC behaviours, is a priority. Using viable isolated with oHSV1‑hTERT‑GFP virus coupled fluorescence‑activated sorting (FACS), whole genome was amplified using multiple annealing looping‑based...
Abstract T cells and cell receptors (TCRs) play pivotal roles in adaptive immune responses against tumors. The development of next-generation sequencing technologies has enabled the analysis TCRβ repertoire usage. Given scarce investigations on TCR lung cancer tissues, this study, we analyzed repertoires tissues matched distant non-tumor (normal tissues) from 15 patients. Based our results, general distribution clones was similar between normal tissues; however, proportion highly expanded...