A5002306566- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Acute Lymphoblastic Leukemia research
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Outbreaks Research
- COVID-19 Clinical Research Studies
- Autoimmune and Inflammatory Disorders Research
- Gene expression and cancer classification
- Epigenetics and DNA Methylation
- SARS-CoV-2 detection and testing
- Respiratory viral infections research
- Molecular Biology Techniques and Applications
- HIV Research and Treatment
- Influenza Virus Research Studies
- Chronic Lymphocytic Leukemia Research
- Acute Myeloid Leukemia Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Chronic Myeloid Leukemia Treatments
- CRISPR and Genetic Engineering
- Viral Infections and Immunology Research
- Psoriasis: Treatment and Pathogenesis
- Immune cells in cancer
- Lung Cancer Treatments and Mutations
- Biomarkers in Disease Mechanisms
- Immune responses and vaccinations
Griffith University
2017-2024
Global Virus Network
2023
The Kids Research Institute Australia
2005-2009
Children's Cancer and Leukaemia Group
2005-2008
The University of Western Australia
2006-2007
Hospital de São João
1997
The recent emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the underlying cause Disease 2019 (COVID-19), has led to a worldwide pandemic causing substantial morbidity, mortality, and economic devastation. In response, many laboratories have redirected attention SARS-CoV-2, meaning there is an urgent need for tools that can be used in unaccustomed working with coronaviruses. Here we report range SARS-CoV-2 research. First, describe facile single plasmid reverse...
The use of microarray technology to assess gene expression levels is now widespread in biology. validation results using independent mRNA quantitation techniques remains a desirable element any experiment. To facilitate the comparison data between laboratories it essential that methodologies be critically examined. We have assessed correlation scores obtained for 48 human genes oligonucleotide microarrays and same measured by quantitative real-time RT-PCR (qRT-PCR). Correlations with qRT-PCR...
Glucocorticoids (GCs) are among the most important drugs for acute lymphoblastic leukaemia (ALL), yet despite their clinical importance, exact mechanisms involved in GC cytotoxicity and development of resistance remain uncertain. We examined baseline profile a panel T-ALL cell lines to determine factors that contribute without prior drug selection. Transcriptional profiling indicated is associated with proliferative phenotype involving upregulation glycolysis, oxidative phosphorylation,...
SARS-CoV-2 variants, with the threat of increased transmissibility, infectivity, and immune escape, continue to emerge as COVID-19 pandemic progresses. Detailing pathogenesis disease caused by such Delta, is essential better understand clinical emerging variants associated disease.
A Bronquiolite Viral Aguda (BVA) é uma infecção respiratória frequente em lactentes, sendo o Vírus Sincicial Respiratório (VSR) principal agente etiológico. doença pode evoluir para quadros graves, exigindo hospitalização, especialmente grupos de risco. O diagnóstico clínico, com avanços no uso da PCR. tratamento suporte, e novas estratégias, como imunoprofilaxia, estão estudadas. Pesquisas indicam possível relação entre BVA doenças respiratórias futuras, reforçando a necessidade estratégias...
A síndrome metabólica é um conjunto de condições que aumentam o risco doenças cardiovasculares e diabetes tipo 2, caracterizada por obesidade abdominal, resistência à insulina, hipertensão dislipidemia. gestão eficaz envolve intervenções nutricionais, atividade física, farmacoterapia suporte psicológico. adoção mudanças no estilo vida, como dieta equilibrada exercícios, melhora a sensibilidade insulina controle da pressão arterial. O objetivo deste estudo analisar estratégias...
Summary Despite significant improvements in the treatment of childhood acute lymphoblastic leukaemia (ALL), prognosis for relapsing patients remains poor. The aim this study was to generate a transcriptional profile relapsed ALL increase our understanding mechanisms involved therapy failure. RNA extracted from 11 pairs cryopreserved pre‐B bone marrow specimens taken same at diagnosis and relapse, analysed using HG‐U133A microarrays. Relapse overexpressed genes that are with cell growth...
Arthritogenic alphaviruses are positive-strand RNA viruses that cause debilitating musculoskeletal diseases affecting millions worldwide. A recent discovery identified the four-and-a-half-LIM domain protein 1 splice variant (FHL1A) as a crucial host factor interacting with hypervariable (HVD) of chikungunya virus (CHIKV) nonstructural 3 (nsP3). Here, we show acute and chronic disease in humans correlates elevated levels FHL1. We generated FHL1-/- mice, which when infected CHIKV or...
To develop COVID-19 countermeasures, powerful research tools are essential. We produced a SARS-COV-2 reverse genetic (RG) infectious clone toolkit that will benefit variety of investigations.
Our previous investigation of the nucleolar localisation sequence (NoLS) chikungunya virus (CHIKV) capsid protein demonstrated role in CHIKV virulence. Mutating NoLS led to development a unique live-attenuated vaccine candidate, termed CHIKV-NoLS. CHIKV-NoLS-immunised mice developed long-term immunity from infection after single dose. To further evaluate CHIKV-NoLS attenuation and suitability as vaccine, we examined footpad inoculated for underlying CHIKV-NoLS-induced immunopathology by...
Barmah Forest virus (BFV) is a mosquito-borne that causes arthralgia with accompanying rash, fever, and myalgia in humans. The mainly found Australia has caused outbreaks associated significant health concerns. As the sole representative of complex within genus
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) continues its significant health and economic impact globally. Despite the success of spike-protein vaccines in preventing severe disease, long-lasting protection against emerging variants prevention breakthrough infections transmission remain elusive. We generate an intranasal live-attenuated SARS-CoV-2 vaccine, CDO-7N-1, using codon deoptimization. CDO-7N-1 shows highly attenuated replication minimal or no lung...
Summary In recent years microarrays have been used extensively to characterize gene expression in acute lymphoblastic leukaemia (ALL). Few studies, however, analysed normal haematopoietic cell populations identify altered ALL. We oligonucleotide compare the profile of paediatric precursor‐B (pre‐B) ALL specimens with two control populations, CD34 + and CD19 IgM − cells, focus on genes linked leukemogenesis. A set eight was identified a ninefold higher average compared cells. All these were...
Arthritogenic alphaviruses cause debilitating inflammatory disease, and current therapies are restricted to palliative approaches. Here, we show that following monocyte-driven muscle inflammation, tissue recovery is associated with the accumulation of CX 3 CR1 + macrophages in muscle. Modulating monocyte infiltration using immune-modifying microparticles (IMP) reduced damage inflammation enhanced formation repair-associated This shows modulating key effectors viral can alter outcome disease...
Chikungunya virus (CHIKV) is the causative pathogen of chikungunya fever, a mosquito-borne viral disease causing highly debilitating arthralgia that can persist for months and progress to chronic arthritis. Our previous studies have identified CHIKV live-attenuated vaccine candidate CHIKV-NoLS. Like most vaccines, attenuated replication CHIKV-NoLS has potential limit scalable production. To overcome production limits, as well other drawbacks we developed an in vivo liposome-RNA delivery...
Arthritogenic alphaviruses are mosquito-borne viruses that a major cause of infectious arthropathies worldwide, and recent outbreaks chikungunya virus Ross River (RRV) infections highlight the need for robust intervention strategies. Alphaviral arthritis can persist months after initial acute disease, is mediated by cellular immune responses. A common strategy to limit inflammation pathology dampen overwhelming inflammatory responses modulating proinflammatory cytokine pathways. Here, we...
Summary The in vitro efficacies of three new drugs – clofarabine (CLOF), nelarabine (NEL) and flavopiridol (FP) were assessed a panel acute lymphoblastic leukaemia (ALL) cell lines. 50% inhibitory concentration (IC50) for CLOF across all lines was 188‐fold lower than that NEL. B‐lineage, but not T‐lineage lines, >7‐fold more sensitive to cytosine arabinoside (ARAC). NEL IC50 25‐fold 113‐fold higher ARAC T‐ respectively. T‐ALL cells eightfold B‐lineage there considerable overlap. FP potent...
Cell lines are important models for drug resistance in acute lymphoblastic leukaemia (ALL), but often criticised as being unrepresentative of primary disease. There also doubts regarding the authenticity many lines. We have characterised a panel ALL cell growth and compared data with that published patient specimens. In contrast to convention highly proliferative, those established our laboratory grow at rates similar estimates leukaemic cells vivo (doubling time 53–442 h). Authenticity was...
Summary Despite high cure rates 25% of children with acute lymphoblastic leukaemia (ALL) relapse and have dismal outcome. Crucially, many are currently stratified as standard risk (SR) additional markers to improve patient stratification required. Here we used diagnostic bone marrow specimens from 101 pre‐B ALL examine the use gene expression profiles (GEP) predictors long‐term clinical Patients were divided into two cohorts for model development validation based on availability specimen...
Arthritogenic alphaviruses, such as Ross River virus (RRV) and chikungunya (CHIKV), particularly affect joints of the extremities can lead to debilitating potentially chronic polyarthritis/polyarthralgia. The innate immune response host plays a crucial role in inducing proinflammatory factors, leading tissue destruction bone loss joints. This study was performed assess how inhibition interleukin-1β (IL-1β) signaling using clinical rheumatoid arthritis drug anakinra influences mice with...
Summary In the last four decades survival of patients with newly diagnosed childhood T‐cell acute lymphoblastic leukaemia (T‐ALL) has improved dramatically. sharp contrast, relapsed T‐ALL continues to confer a dismal prognosis. We sought determine if gene expression profiling could uncover signature outcome for children T‐ALL. Using 12 patient specimens obtained before therapy started, we examined profile by oligonucleotide microarrays. identified three genes, CFLAR , NOTCH2 and BTG3 whose...
Arthritogenic alphaviruses are transmitted via mosquito bites through the skin, potentially causing debilitating diseases. Our understanding of how viral infection starts in skin and virus systemically disseminates to cause disease remains limited.
There is an unmet medical need for effective anti-inflammatory agents the treatment of acute and post-acute lung inflammation caused by respiratory viruses. The semi-synthetic polysaccharide, Pentosan polysulfate sodium (PPS), inhibitor NF-kB activation, was investigated its systemic local effects in a mouse model influenza virus A/PR8/1934 (PR8 strain) mediated infection. Immunocompetent C57BL/6J mice were infected intranasally with sublethal dose PR8 treated subcutaneously 3 or 6 mg/kg PPS...