A5059454424- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Cancer, Hypoxia, and Metabolism
- Nanoplatforms for cancer theranostics
- Estrogen and related hormone effects
- RNA Interference and Gene Delivery
- Radiopharmaceutical Chemistry and Applications
- Thyroid Cancer Diagnosis and Treatment
- Adipose Tissue and Metabolism
- bioluminescence and chemiluminescence research
- Nanoparticle-Based Drug Delivery
- Immune cells in cancer
- Medical Imaging Techniques and Applications
- S100 Proteins and Annexins
- Metabolism, Diabetes, and Cancer
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Kruppel-like factors research
- Cancer, Stress, Anesthesia, and Immune Response
- Cytomegalovirus and herpesvirus research
- Neonatal Respiratory Health Research
- Biochemical Acid Research Studies
- RNA modifications and cancer
- Radiation Therapy and Dosimetry
Daegu-Gyeongbuk Medical Innovation Foundation
2017-2024
Kyungpook National University Hospital
2013-2023
Gyeongbuk Marine Bio-Industry Research Institute
2021
Institute of Laboratory Animal Science
2020
Kyungpook National University
2009-2019
Government of the Republic of Korea
2019
Pohang University of Science and Technology
2014
Kyung Hee University
2014
Chosun University
2014
Michigan United
2013
Abstract Central serotonin (5-HT) is an anorexigenic neurotransmitter in the brain. However, accumulating evidence suggests peripheral 5-HT may affect organismal energy homeostasis. Here we show regulates white and brown adipose tissue function. Pharmacological inhibition of synthesis leads to lipogenesis epididymal (WAT), induction browning inguinal WAT activation adaptive thermogenesis (BAT). Mice with inducible Tph1 KO tissues exhibit a similar phenotype as mice which inhibited...
Triple-negative breast cancer (TNBC) has a high risk of relapse and there are few chemotherapy options. Although 5-hydroxytryptamine (5-HT, serotonin) signaling pathways have been suggested as potential targets for anti-cancer drug development, the mechanism responsible action 5-HT in TNBC remains unknown.Quantitative real-time polymerase chain reaction (qRT-PCR) Western blotting were used to measure mRNA protein levels, respectively. Cell proliferation was measured using CellTiter 96...
CXCR4, the stromal cell-derived factor-1 receptor, plays an important role in migration of hematopoietic progenitor/stem cells to injured and inflamed areas. Noninvasive cell tracking methods could be useful for monitoring fate. Therefore, this study, we evaluated efficacy intravenous infusion genetically engineered mesenchymal stem (MSCs) overexpressing CXC chemokine receptor 4 (CXCR4) home tumor, by optical imaging. We constructed a retroviral vector containing CXCR with dual reporter...
Radionuclide-embedded gold nanoparticles (RIe-AuNPs) were developed as a highly sensitive and stable nuclear optical imaging agent for efficient dendritic cell (DC)-based immunotherapy tracking of DC-migration to lymph nodes. The RIe-AuNPs synthesized via simple straightforward DNA-based radiolabeling chemistry additional Au shell formation strategies, leading high radiosensitivity excellent in vivo stability. exert no adverse effects on the biological functions DCs, labeled DCs show strong...
The metabolism of glutamine and glucose is recognized as a promising therapeutic target for the treatment cancer; however, targeted molecules that mediate in cancer cells have not been addressed. Here, we show restricting supply hepatoma cells, including HepG2 Hep3B markedly increased expression retinoic acid‐related orphan receptor alpha (RORα). Up‐regulation RORα glutamine‐deficient resulted from an increase level cellular reactive oxygen species nicotinamide adenine dinucleotide...
The development of highly sensitive, stable, and biocompatible imaging agents allowing visualization dendritic cell (DC) migration is one the essential factors for effective DC-based immunotherapy. Here, we used a novel efficient synthesis approach to develop radioiodine-124-labeled tannic acid gold core–shell nanoparticles (124I-TA-Au@AuNPs) DC labeling in vivo tracking their using positron emission tomography (PET). 124I-TA-Au@AuNPs were produced within 40 min high yield via...
Abstract Triple-negative breast cancer (TNBC) is a highly metastatic with poor prognosis. In the present study, we demonstrated that Src, non-receptor tyrosine kinase, might provide an effective therapeutic strategy to overcome TNBC invasion and metastasis, which are mediated via synergistic action of lysosomal enzyme cathepsin S (CTSS) gelatinase MMP-9. Knock-down MMP-9 CTSS using siRNAs resulted in suppression MDA-MB-231 cell invasion, was similarly observed pharmacological inhibitors....
Isocitrate dehydrogenase 2 (IDH2) is an NADP
Abstract We sought to evaluate the feasibility of molecular imaging using human sodium iodide symporter (hNIS) gene as a reporter, in addition enhanced firefly luciferase (effluc) gene, for tracking dendritic cell (DCs) migration living mice. A murine line (DC2.4) co-expressing hNIS and effluc genes (DC/NF) was established. For DC-tracking study, mice received either parental DCs or DC/NF cells left right footpad, respectively combined I-124 PET/CT bioluminescence (BLI) were performed. In...
In fasting or diabetes, gluconeogenic genes are transcriptionally activated by glucagon stimulation of the cAMP-protein kinase A (PKA)–CREB signaling pathway. Previous work showed pyruvate dehydrogenase (PDK) inhibition in skeletal muscle increases oxidation, which limits availability substrates liver. However, this study found upregulation hepatic PDK4 promoted glucagon-mediated expression genes, whereas knockdown caused opposite effect on gene and decreased glucose production....
The aim of this study was to investigate the feasibility nuclear molecular imaging using human sodium iodide symporter (hNIS) as a reporter gene monitor macrophage migration toward inflammatory foci. <b>Methods:</b> A stable cell line coexpressing hNIS and green fluorescent protein (GFP) genes (RAW264.7/hNIS-GFP R<sub>NIS</sub> cell) established from an immortalized (RAW264.7 cells). <sup>125</sup>I uptake determined (for functional activity), flow cytometry analysis (to examine GFP...
In addition to their degradative role in protein turnover, proteases play a key as positive or negative regulators of signal transduction pathways and therefore dysregulation contributes many disease states. Regulatory roles include hormone-like triggering G protein-coupled signaling (Protease-Activated-Receptors); shedding ligands such EGF, Notch Fas; events that lead apoptotic cell death. Dysregulated activation apoptosis by the caspase family has been linked diseases cancer, autoimmunity...
New imaging probes with high sensitivity and stability are urgently needed to accurately detect sentinel lymph nodes (SLNs) for successful cancer diagnosis. Herein, the use of highly sensitive stable PEGylated radionuclide-embedded gold nanoparticles (PEG-RIe-AuNPs) is reported detection SLNs by combined positron emission tomography Cerenkov luminescence (PET/CLI). PEG-RIe-AuNPs show both in vitro vivo, not toxic normal ovarian immune cells. In vivo PET/CLI clearly reveals as early 1 h post...
Abstract Plasmonic nanostructure-mediated photothermal therapy (PTT) has proven to be a promising approach for cancer treatment, and new approaches its effective delivery tumor lesions are currently being developed. This study aimed assess macrophage-mediated of PTT using radioiodine-124-labeled gold nanoparticles with crushed shells ( 124 I-Au@AuCBs) as theranostic nanoplatform. I-Au@AuCBs exhibited conversion effects both in vitro vivo were efficiently taken up by macrophages without...
Emerging evidence suggest that transcription factors play multiple roles in the development of pancreatitis, a necroinflammatory condition lacking specific therapy. Estrogen-related receptor γ (ERRγ), pleiotropic factor, has been reported to vital role pancreatic acinar cell (PAC) homeostasis. However, ERRγ PAC dysfunction remains hitherto unknown. Here, we demonstrated both mice models and human cohorts pancreatitis is associated with an increase gene expression via activation STAT3....
Sorafenib (BAY 43-9006) was developed as a multi-kinase inhibitor to treat advanced renal cell, hepatocellular, and thyroid cancers. The cytotoxic effect of sorafenib on cancer cells results from not only inhibiting the MEK/ERK signaling pathway (the on-target effect) but also inducing oxidative damage off-target effect). inhibitory system Xc
Reliable and sensitive imaging tools are required to track macrophage migration provide a better understating of their biological roles in various diseases. Here, we demonstrate the possibility radioactive iodide-embedded gold nanoparticles (RIe-AuNPs) as cell tracker for nuclear medicine imaging. To this utility, monitored carrageenan-induced sites acute inflammation living subjects visualized effects anti-inflammatory agents on process. Macrophage labeling with RIe-AuNPs did not alter...
Radioactive isotope-labeled gold nanomaterials have potential biomedical applications. Here, we report the synthesis and characterization of PEGylated crushed shell-radioactive iodide-124-labeled core nanoballs (PEG-124I-Au@AuCBs) for in vivo tumor imaging applications through combined positron emission tomography Cerenkov luminescent (PET/CLI). PEG-124I-Au@AuCBs showed high stability sensitivity various pH solutions, serum, conditions were not toxic to tested cells. Combined PET/CLI clearly...
Recently, 18F-tetrafluoroborate (TFB) was used as a substrate for the human sodium/iodide symporter (hNIS) reporter gene. This study evaluated feasibility of performing molecular-genetic imaging by using new radiotracer (18F-TFB) hNIS gene, to track dendritic cell (DC) migration in live mice. A murine line (DC2.4) co-expressing and effluc genes (DC/NF) established. To confirm functional cellular expression both NIS inoculated DC/NF cells bio-medical imaging, combined bioluminescence (BLI)...
Abstract Purpose: New strategies to restore sodium iodide symporter (NIS) expression and function in radioiodine therapy–refractive anaplastic thyroid cancers (ATCs) are urgently required. Recently, we reported the regulatory role of estrogen-related receptor gamma (ERRγ) ATC cell NIS function. Herein, identified DN200434 as a highly potent (functional IC50 = 0.006 μmol/L), selective, orally available ERRγ inverse agonist for enhancement ATC. Experimental Design: We sought identify better...
Background Advanced cancer therapy is targeted at primary tumors and also recurrent or metastatic cancers. Combinational treatment has recently shown high efficiency against In this study, we synthesized a thermal responsive hybrid nanoparticle (TRH) containing FimH, an immune stimulatory recombinant protein, for the induction of combination photothermal (PTT) immunotherapy its metastasis. Methods The was incorporated with near-infrared (NIR) absorbent, indocyanine green, decorated FimH on...
AbstractFirefly luciferase (Fluc) has been widely used as a reporter gene. The aim of this study was to investigate immune response protein after an intradermal injection pcDNA3.1-Fluc in immunocompetent BALB/c mice. We observed bioluminescence at sites from 1 day 7 days post-injection when intradermally injected into ear-pinnae. To observe induced response, the percentages CD8+IFN-γ+ cells draining lymphoid mice immunized by were measured. And tumor growths CT26/Fluc group monitored...