A5023061311- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Transplantation: Methods and Outcomes
- Celiac Disease Research and Management
- Microscopic Colitis
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- Gastrointestinal motility and disorders
- Hematopoietic Stem Cell Transplantation
- SARS-CoV-2 and COVID-19 Research
- Digestive system and related health
- RNA Interference and Gene Delivery
- Drug-Induced Adverse Reactions
- Reproductive System and Pregnancy
- Congenital gastrointestinal and neural anomalies
- Infant Nutrition and Health
- Psoriasis: Treatment and Pathogenesis
- Dermatology and Skin Diseases
- vaccines and immunoinformatics approaches
- CAR-T cell therapy research
- Cytomegalovirus and herpesvirus research
- Pulmonary Hypertension Research and Treatments
- Immunodeficiency and Autoimmune Disorders
Policlinico San Matteo Fondazione
2018-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2018-2024
University of Pavia
2018-2020
The identification of CD4+ T cell epitopes is instrumental for the design subunit vaccines broad protection against coronaviruses. Here, we demonstrate in COVID-19-recovered individuals a robust response to naturally processed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein and nucleoprotein (N), including effector, helper, memory cells. By characterizing 2943 S-reactive clones from 34 individuals, found that receptor-binding domain (RBD) highly immunogenic 33%...
Background: Growing evidence suggests that an altered microbiota composition contributes to the pathogenesis and clinical features in celiac disease (CD). We performed a comparative analysis of gut adulthood CD evaluate whether: (i) dysbiosis anticipates mucosal lesions, (ii) gluten-free diet restores eubiosis, (iii) refractory has peculiar microbial signature, (iv) salivary fecal communities overlap one. Methods: This is cross-sectional study where total 52 patients, including 13 active CD,...
Enteric glial cells (EGCs), the major components of enteric nervous system (ENS), are implicated in maintenance gut homeostasis, thereby leading to severe pathological conditions when impaired. However, due technical difficulties associated with EGCs isolation and cell culture that results a lack valuable vitro models, their roles physiological contexts have been poorly investigated so far. To this aim, we developed for first time, human immortalized EGC line (referred as ClK clone) through...
Abstract Background CLAD (Chronic Lung Allograft Dysfunction) remains a serious complication following lung transplantation. Some evidence shows that portions of Extracorporeal Photopheresis (ECP)-treated patients improve/stabilize their graft function. In spite that, data concerning molecular mechanisms are still lacking. Aims our study were to assess whether ECP effects mediated by Mononuclear Cells (MNCs) modulation in term microRNAs (miRNAs) expression and growth factors release. Methods...
The limited efficacy of Natural Killer (NK) cell-based immunotherapy results in part from the suboptimal expansion and persistence infused cells. Recent reports suggest that generation NK cells with memory-like properties upon vitro activation defined cytokines might be an effective way ensuring long-lasting cell function vivo. Here, we demonstrate IL-12, IL-15 IL-18 followed by a one-week culture optimal doses Interleukin (IL-2) generates substantial numbers able to persist for at least...
Clinical evidence suggests an improvement or stabilization of lung function in a fraction patients with bronchiolitis obliterans syndrome (BOS) treated by extracorporeal photopheresis (ECP); however, few studies have explored the epigenetic and molecular regulation this therapy. The aim present study was to evaluate whether specific set miRNAs were significantly regulated ECP. Total RNA isolated from serum established BOS grade 1–2 prior start after 6 months ECP treatment. We observed...
Introduction: Allele-level donor–recipient match at HLA-A, HLA-B, HLA-C and HLA-DRB1 loci impacts the outcome after cord blood transplantation for hematologic malignancies modifies strategy of donor selection. High definition both class I II HLA time listing is a way to improve attractiveness bank inventories, reducing search procurement simplifying choice, in particular, patients non-European heritage. Methods: In 2014, Luminex ® xMAP technology was introduced our laboratory practice...
Abstract Background and Aims Different population genetics studies showed that interactions between killer‐cell immunoglobulin‐like receptors (KIR) HLA play a role in viral disease outcome, but functional correlates are missing. Building upon our previous work pointing to regulatory for KIR3DL1/DS1 hepatitis C virus (HCV) infection, we analysed whether its expression may affect natural killer (NK) cell function the presence or absence of principal ligand HLA‐Bw4 KIR haplotype A B carriers,...
Duodenal villous atrophy is due not only to coeliac disease and its complications but also other rare enteropathies unrelated gluten consumption, defined as noncoeliac enteropathies. The diagnosis of remains challenging, HLA typing has been widely used exclude if DQ2 DQ8 alleles are absent. However, the frequency various in still unknown.To describe genetic profile patients affected by who have evaluated at our centres between 2000 2021, investigate diagnostic role typing.Genomic DNA was...