A5112077872- Neuroscience and Neuropharmacology Research
- Atherosclerosis and Cardiovascular Diseases
- Nerve injury and regeneration
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Neurogenesis and neuroplasticity mechanisms
- Neural dynamics and brain function
- T-cell and B-cell Immunology
- Lipid Membrane Structure and Behavior
- Cell Adhesion Molecules Research
- Adenosine and Purinergic Signaling
- Renal Diseases and Glomerulopathies
- Neuropeptides and Animal Physiology
- Systemic Lupus Erythematosus Research
- Axon Guidance and Neuronal Signaling
- Neurotransmitter Receptor Influence on Behavior
- Genetic Associations and Epidemiology
- Genetic Syndromes and Imprinting
- Single-cell and spatial transcriptomics
- Calpain Protease Function and Regulation
- Pain Mechanisms and Treatments
- Galectins and Cancer Biology
- Glaucoma and retinal disorders
- Visual perception and processing mechanisms
- Cytokine Signaling Pathways and Interactions
University of Coimbra
2002-2025
University of Geneva
2014-2022
Foundation for laboratory medicine
2020-2022
University Hospital of Geneva
2019
Radboud University Medical Center
2017
Radboud Institute for Molecular Life Sciences
2017
Institute of Social and Preventive Medicine
2017
Radboud University Nijmegen
2017
Columbia University Irving Medical Center
2013-2016
Universidade Estadual Paulista (Unesp)
2009-2012
Increased levels of the urinary albumin-to-creatinine ratio (UACR) are associated with higher risk kidney disease progression and cardiovascular events, but underlying mechanisms incompletely understood. Here, we conduct trans-ethnic (n = 564,257) European-ancestry specific meta-analyses genome-wide association studies UACR, including ancestry- diabetes-specific analyses, identify 68 UACR-associated loci. Genetic correlation analyses score associations in an independent electronic medical...
Brain-derived neurotrophic factor (BDNF) plays an important role in synaptic plasticity the hippocampus, but mechanisms involved are not fully understood. The neurotrophin couples activation to changes gene expression underlying long term potentiation and short plasticity. Here we show that BDNF acutely up-regulates GluR1, GluR2, GluR3 α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor subunits 7-day tropomyosin-related kinase vitro cultured hippocampal neurons. increase...
The nervous system transmits signals between neurons via neurotransmitter release during synaptic vesicle fusion. In order to observe uptake and from individual presynaptic terminals directly, we designed fluorescent false neurotransmitters as substrates for the monoamine transporter. Using these probes image dopamine in striatum, made several observations pertinent plasticity. We found that fraction of vesicles releasing per stimulus was dependent on frequency. A kinetically distinct...
(1) Background: Monocytes and nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome orchestrate lipid-driven amplification of vascular inflammation promoting the disruption fibrous cap. The components NLRP3 are expressed in macrophages foam cells within human carotid atherosclerotic plaques VSMCs hypertension. Whether monocytes activation direct triggers VSMC phenotypic switch plaque need to be investigated. (2) Methods: effect oxLDL-activated co-cultured...
Localization of Trk neurotrophin receptors is an important factor in directing cellular communication developing and mature neurons. One potential site action lipid raft membrane microdomains. Although have been localized to rafts, little known about how these are directed there or localization microdomains regulates signaling. Here, we report that the TrkB brain-derived neurotrophic (BDNF) receptor specifically intracellular rafts cortical hippocampal membranes response BDNF this process...
We recently introduced fluorescent false neurotransmitters (FFNs) as optical tracers that enable the visualization of neurotransmitter release at individual presynaptic terminals. Here, we describe a pH-responsive FFN probe, FFN102, which polar dopamine transporter substrate selectively labels cell bodies and dendrites in ventral midbrain dopaminergic synaptic terminals dorsal striatum. FFN102 exhibits greater fluorescence emission neutral than acidic environments, thus affords means to...
Neurotrophins, such as nerve growth factor and brain-derived neurotrophic factor, activate Trk receptor tyrosine kinases through dimerization at the cell surface followed by autophosphorylation recruitment of intracellular signaling molecules. The pathways used neurotrophins share many common protein substrates that are other (RTK), Shc, Grb2, FRS2, phospholipase C-γ. Here we describe a novel RTK mechanism involves 220-kilodalton membrane tetraspanning protein, ARMS/Kidins220, which is...
Brain-derived neurotrophic factor (BDNF) signaling is critical for neuronal development and transmission. Recruitment of TrkB receptors to lipid rafts has been shown be necessary the activation specific pathways modulation neurotransmitter release by BDNF. Since are known modulated adenosine A<sub>2A</sub> receptor activation, we hypothesized that could influence localization among different membrane microdomains. We found agonists increased levels in raft fraction cortical membranes...
Background: Atherosclerosis is a chronic inflammatory disease where macrophages participate in the progression of disease. However, role resident-like (res-like) atherosclerotic aorta not completely understood. Methods: A single-cell RNA sequencing analysis CD45+ leukocytes apolipoprotein E–deficient (Apoe−/−) mice on normal cholesterol diet (NCD) or high (HCD), respecting side-to-specific predisposition to atherosclerosis, was performed. population res-like expressing hyaluronan receptor...
(1) Background: Vascular smooth muscle cells (VSMCs) undergo a complex phenotypic switch in response to atherosclerosis environmental triggers, contributing disease progression. However, the heterogeneity of VSMCs and how VSMC dedifferentiation affects human carotid artery (CAD) risk has not been clearly established. (2) Method: A single-cell RNA sequencing analysis CD45- derived from atherosclerotic aorta Apolipoprotein E-deficient (Apoe-/-) mice on normal cholesterol diet (NCD) or high...
Abstract Brain‐derived neurotrophic factor (BDNF) modulates glutamatergic excitatory transmission in hippocampal primary cultures by acting at a presynaptic locus. Although it has been suggested that BDNF also adult hippocampus transmission, this remains matter of controversy. To clarify putative role for neurotrophin the modulation glutamate release we applied exogenous to isolated rat nerve terminals. BDNF, 100 ng/ml, potentiated 25% K + ‐evoked [ 3 H]glutamate from synaptosomes. The small...
B cell functions in the process of atherogenesis have been investigated but several aspects remain to be clarified.
Background: Atherosclerosis is a chronic inflammatory vascular disease and the main cause of death morbidity. Emerging evidence suggests that ubiquitination plays an important role in pathogenesis atherosclerosis including control inflammation, smooth muscle cell (VSMC) function atherosclerotic plaque stability. Peli1 type E3 ubiquitin ligase has emerged as critical regulator innate adaptive immunity, however, its remains to be elucidated. Methods: Apoe−/− mice Peli1-deficient Peli1−/− were...