A5036231024- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- RNA modifications and cancer
- Microfluidic and Bio-sensing Technologies
- Cancer-related molecular mechanisms research
- Ferroptosis and cancer prognosis
- Epigenetics and DNA Methylation
- Lung Cancer Diagnosis and Treatment
- Brain Metastases and Treatment
- Glioma Diagnosis and Treatment
- Phagocytosis and Immune Regulation
- Microfluidic and Capillary Electrophoresis Applications
- Cancer-related gene regulation
- Immune cells in cancer
- Radiomics and Machine Learning in Medical Imaging
- Glycosylation and Glycoproteins Research
- Nanoparticle-Based Drug Delivery
- Innovative Microfluidic and Catalytic Techniques Innovation
- Bone Tissue Engineering Materials
- ATP Synthase and ATPases Research
- Metabolomics and Mass Spectrometry Studies
- Lung Cancer Research Studies
- Inhalation and Respiratory Drug Delivery
- COVID-19 diagnosis using AI
- Genetic Syndromes and Imprinting
Dalian Medical University
2016-2025
Second Affiliated Hospital of Dalian Medical University
2016-2025
Harbin Medical University
2024
Fourth Affiliated Hospital of Harbin Medical University
2024
Metastasis is a complex pathophysiological process. As the main cause of cancer mortality in humans it represents serious challenge to both basic researchers and clinicians. Here we report design construction multi-organ microfluidic chip that closely mimics vivo microenvironment lung metastasis. This multi-organs-on-a-chip includes an upstream "lung" three downstream "distant organs", with polydimethylsiloxane (PDMS) layers two thin PDMS microporous membranes bonded form parallel...
Epidermal growth factor receptor (EGFR) genotype is crucial for treatment decision making in lung cancer, but it can be affected by tumour heterogeneity and invasive biopsy during gene sequencing. Importantly, not all patients with an EGFR mutation have good prognosis EGFR-tyrosine kinase inhibitors (TKIs), indicating the necessity of stratifying EGFR-mutant genotype. In this study, we proposed a fully automated artificial intelligence system (FAIS) that mines whole-lung information from CT...
Abstract CD47 is overexpressed in many human cancers, its level positively correlates with tumor invasion and metastasis. However, it largely unknown whether overexpression drives metastasis how lead to non-small cell lung cancer (NSCLC). In this study, we analyzed NSCLC specimens lines revealed that expressed at a higher than tumor-free control samples. Furthermore, increased expression correlated clinical staging, lymph node distant order understand the molecular mechanisms underlying...
Abstract The mechanism by which tumor-associated macrophages (TAMs) affect cancer progression is not fully understood. This study developed a microfluidic-based co-culture device to mimic the tumor microenvironment assess TAM effects on invasion and metastasis in NSCLC. results showed lung carcinoma cells could cause show M2 (a TAM-like) phenotype, these promoted cell EMT invasion. Proteomic analysis iTRAQ quantitation strategy GO ontology of indicated that αB-Crystallin (CRYAB) might be...
Abstract Background Platinum‐based chemotherapy is effective in inducing shrinkage of primary lung cancer lesions; however, it shows finite therapeutic efficacy patients suffering from brain metastasis (BM). The intrinsic changes BM cells, which contribute to the poor results remain unknown. Methods Platinum drug‐sensitivity was assessed by utilizing a preclinical model PC9 adenocarcinoma cells vitro and vivo. High consumption glutathione (GSH) two associated upregulated proteins (GPX4...
Abstract Cancer-associated fibroblasts (CAFs) contribute to tumour epithelial-mesenchymal transition (EMT) via interaction with cancer cells. However, the molecular mechanisms underlying tumour-promoting EMT of CAFs in lung adenocarcinoma (ADC) remain unclear. Here, we observed that isolated from ADC promoted production stromal cell-derived factor-1 (SDF-1) conditioned medium (CM). CAF-derived SDF-1 enhanced invasiveness and by upregulating CXCR4, β-catenin, PPARδ, while downregulating these...
Sox2 and Oct4 are transcription factors with the characteristics of regulating self-renewal differentiation embryonic stem cell. The aim this study was to detect expression analyze their clinical significance in human non-small-cell lung cancer (NSCLC). Expression were assayed tissues corresponding paracancerous from 44 patients NSCLC 21 benign tumors using immunohistochemistry, Western blot, reverse polymerase chain reaction (RT-PCR). correlation between tumor type, grade prognosis utility...
Tumor stroma and growth factors provide a survival environment to tumor cells can modulate their chemoresistance by dysregulating several signal pathways. In this study, we fabricated three-dimensional (3D) microfluidic chip using polydimethylsiloxane (PDMS) investigate the impact of hepatocyte factor (HGF) from cancer-associated fibroblasts (CAF) on Met/PI3K/AKT activation, glucose regulatory protein (GRP78) expression paclitaxel-induced A549 cell apoptosis. With concentration gradient...
Abstract Recently there has been a more focus on the development of an efficient technique for detection circulating tumor cells (CTCs), due to their significance in prognosis and therapy metastatic cancer. However, it remains challenge because low count CTCs blood. Herein, rapid high-sensitivity approach using integrated microfluidic system, consisting deterministic lateral displacement (DLD) isolating structure, automatic purifying device with CD45-labeled immunomagnetic beads capturing...
Brain metastasis (BM) is common among cases of advanced non-small cell lung cancer (NSCLC) and the leading cause death for these patients. Mesothelin (MSLN), a tumor-associated antigen expressed in many solid tumors, has been reported to be involved progression multiple tumors. However, its potential involvement BM NSCLC underlying mechanism remain unknown. The expression MSLN was validated clinical tissue serum samples using immunohistochemistry enzyme-linked immunosorbent assay. ability...
Invadopodia or invasive feet, which are actin-rich membrane protrusions with matrix degradation activity formed by cancer cells, a key determinant in the malignant progression of tumors and represent an important target for therapies. In this work, we presented microfluidic 3D culture device continuous supplement fresh media via syringe pump. The mimicked tumor microenvironment vivo could be used to assay invadopodia formation study mechanism human lung invasion. With device, investigated...
The lack of non-invasive methods for detection early micro-metastasis is a major cause the poor prognosis non-small cell lung cancer (NSCLC) brain metastasis (BM) patients. Herein, we aimed to identify circulating biomarkers based on proteomics diagnosis and monitoring patients with NSCLC BM.Upregulated proteins were detected by secretory in animal-derived high metastatic line. A well-designed study composed three independent cohorts was then performed verify these blood-based protein...
Abstract It is desirable to have a biomarker which can facilitate low‐dose CT in diagnosis of early stage lung cancer. CTAPIII/CXCL7 reported be potential for In this study, we investigated the serum level patients at different cancer and diagnostic efficacy NSCLC. The plasma was assayed by ELISA. CEA, SCCAg, Cyfra211 were measured using commercial chemiluminescent microparticle immunoassay. A total 419 subjects recruited, including 265 NSCLC 154 healthy individuals. randomly assigned...
Abstract Background Gastric cancer (GC) is the fourth leading cause of cancer‐related death worldwide. Minichromsome maintenance proteins family member 8 (MCM8) assists DNA repair and replication. MCM8 exerts tumor promotor function in multiple digestive system tumors. also considered as a potential therapeutic target. Methods Bioinformatics methods were used to analyze expression clinicopathological significance. was detected by immunohistochemistry (IHC) staining qRT‐PCR. functions GC cell...
Circulating tumor cells (CTCs) represent invasive cell populations and provide a noninvasive solution to the clinical management research of tumors. Characterization CTCs at single-cell resolution enables comprehensive understanding heterogeneity may benefit diagnosis treatment cancer patients. However, most efforts have been made on enumeration detection CTCs, while little focus has directed study. Herein, an integrated microfluidic platform for isolation analysis was established. After...
// Encheng Li 1,* , Zhiyun Xu Hui Zhao 2,* Sun 1 Lei Wang 3 Zhe Guo Yang Zhancheng Gao 4 and Qi Department of Respiratory Medicine, The Second Affiliated Hospital, Dalian Medical University, Dalian, China 2 Physical Examination Center, Liaoning Provincial Key Laboratory for Micro/Nano Technology, University & Critical Care People's Hospital Peking Beijing, * These authors contributed equally to this work Correspondence to: Wang, email: Gao, Keywords : Macrophages, malignant transformation,...
Abstract Background Early lung cancer detection remains a clinical challenge for standard diagnostic biopsies due to insufficient tumor morphological evidence. As epigenetic alterations precede changes, expression of certain imprinted genes could serve as actionable biomarkers malignant lesions. Results Using the previously established quantitative chromogenic gene in situ hybridization (QCIGISH) method, elevated aberrant allelic GNAS , GRB10 SNRPN and HM13 was observed cancers over benign...
Lung adenocarcinoma (LUAD) is still one of the major causes cancer-related mortality across globe. Therefore, there a dire need to identify early specific and sensitive biomarkers or drug targets LUAD for developing improved diagnosis clinical management. We aimed investigate role methyltransferase-like 7B (METTL7B) on tumor development progression in this study.METTL7B's expression was confirmed two independent cohort samples, including tissues microarray (TMA) via immunohistochemistry...
Circulating tumor cells (CTCs) have been considered as an alternative to tissue biopsy for providing both germline-specific and tumor-derived genetic variations. Single-cell analysis of CTCs enables in-depth investigation heterogeneity individualized clinical assessment. However, common CTC enrichment techniques generally limitations low throughput cell damage. Herein, based on micropore-arrayed filtration membrane microfluidic chip, we established integrated isolation platform with...
Abstract Integration and miniaturization are main advantages of microchip‐based systems. Vertical integration the multiple operations within a multiple‐layer chip is expected to satisfy urgent demand for high‐throughput large‐scale applications. This study aimed at establishing double‐layer integrate including cell culture, identification protein detection viability onto platform systematically supplied with flow fresh medium continuously via syringe pump mimic microenvironment in vivo. With...