A5057834584- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Crystal structures of chemical compounds
- Organometallic Compounds Synthesis and Characterization
- Metal complexes synthesis and properties
- Advanced Drug Delivery Systems
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Lung Cancer Research Studies
- RNA Research and Splicing
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell death mechanisms and regulation
- Lung Cancer Treatments and Mutations
- Endoplasmic Reticulum Stress and Disease
- Drug Solubulity and Delivery Systems
- Protein Tyrosine Phosphatases
- PARP inhibition in cancer therapy
- RNA Interference and Gene Delivery
- Protein Degradation and Inhibitors
- Immune cells in cancer
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Organometallic Complex Synthesis and Catalysis
- Medicinal Plant Pharmacodynamics Research
Hangzhou Medical College
2021-2024
Zhejiang Academy of Medical Sciences
2008-2018
Chinese Academy of Medical Sciences & Peking Union Medical College
2013
Although the DNA damage response (DDR) is associated with radioresistance characteristics of lung cancer cells, specific regulators and underlying mechanisms DDR are unclear. Here, we identified serine proteinase inhibitor clade E member 2 (SERPINE2) as a modulator radiosensitivity in cancer. Cells exhibiting after ionizing radiation show upregulation SERPINE2, SERPINE2 knockdown improves tumor vitro vivo. Functionally, deficiency causes reduction homologous recombination repair, rapid...
Hepatocellular carcinoma(HCC) is one of the most common tumors in world. Human insulin-like growth factor 2(IGF2) mRNA binding protein 2(IGF2BP2) plays an important role progression hepatocellular carcinoma. Additionally, long non-coding RNA(lncRNA) has been confirmed as a key regulator carcinoma occurrence. However, function TRPC7-AS1 not verified The research results revealed that high IGF2BP2 expression was associated with decreased survival rate patients Furthermore, knockdown inhibited...
Abstract Conventional oral therapy for ulcerative colitis (UC) is associated with premature release or degradation of drugs in the harsh gastrointestinal environment, resulting reduced therapeutic effectiveness. Consequently, present study aims to develop a dual‐targeted delivery system nanoparticle‐in‐microparticle (nano‐in‐micro) structure. The prepared Asiatic Acid‐loaded (AA/CDM‐BT‐ALG) has pH‐sensitive properties. Cellular uptake evaluation confirms that nanoparticles exhibit targeted...
Immune-checkpoint blockade (ICB) therapies have been widely used in clinical treatment of cancer patients, but only 20–30% patients benefit from immunotherapy. Therefore, it is important to decipher the molecular mechanism resistance ICB and develop new combined strategies. PD-L1 up-regulation tumor cells contributes occurrence immune escape. Increasing evidence shows that its transcription level affected by multiple factors, which limits objective response rate ICB. Fibroblast growth factor...
Human WBSCR22 gene is involved in tumor metastasis, cell growth and invasion, however, its role chemosensitivity to antitumor agents remains unknown. In this study, we analyzed the TCGA cohort found expression of was significantly elevated human colorectal cancer (CRC) tissue. could be served as an independent risk predictor for overall survival (OS), up-regulated predict unfavorable OS CRC patients. Knockdown sensitized cells oxaliplatin vitro vivo, while overexpression led cellular...
Oral absorption of peptides/proteins is usually compromised by various gastrointestinal tract barriers. To improve delivery efficiency, chitosan-conjugated deoxycholic acid (CS-DCA) coupled with sodium alginate (ALG) was prepared to load insulin into pH-sensitive nanoparticles. The insulin-loaded chitosan-deoxycholic acid/alginate nanoparticles (CDA NPs) were characterized size (143.3 ± 10.8 nm), zeta potential (19.5 1.6 mV), entrapment efficiency (61.14 1.67%), and drug loading (3.36...
Abstract Background Brain metastasis (BM) are a devastating consequence of lung cancer. This study was aimed to screen risk factors for predicting BM. Methods Using an in vivo BM preclinical model, we established series adenocarcinoma (LUAD) cell subpopulations with different metastatic ability. Quantitative proteomics analysis used and identify the differential protein expressing map among subpopulation cells. Q-PCR Western-blot were validate proteins vitro. The candidate measured LUAD...
Acute lung injury (ALI) is a critical inflammatory response syndrome that rapidly develops into acute respiratory distress (ARDS). Currently, no effective therapeutic modalities are available for patients with ALI/ARDS. According to recent studies, inhibiting both the release of pro-inflammatory cytokines and formation reactive oxygen species (ROS) as early possible may be promising therapy ALI.
The Brother of Regulator Imprinted Sites (BORIS, gene symbol CTCFL) has previously been shown to promote colorectal cancer cell proliferation, inhibit apoptosis, and resist chemotherapy. However, it is unknown whether Boris plays a role in the progression situ cancer. Here knockout (KO) mice were constructed. function loss cloned mutation that was retained KO verified by testing its activities lines compared with wild-type gene. reduced incidence severity azoxymethane/dextran sulfate-sodium...
Colorectal cancer (CRC) is one of the cancers with high incidence and mortality rates worldwide. In China, there are approximately 400,000 new CRC cases each year, seriously endangering people's life health. Transforming growth factor β-stimulated clone 22 domain family, member 2 (TSC22D2) widely expression in cancers, but role TSC22D2 still unknown.
Hepatocellular carcinoma (HCC) is one of the most prevalent cancers worldwide with a poor clinical prognosis. Protein phosphatase 1 regulatory subunit 14B (PPP1R14B) an unidentified protein that associated occurrence and development various cancers. Recently, PPP1R14B was found to contribute paclitaxel resistance cell progression in triple-negative breast cancer; however, role HCC unknown. Here, we highly expressed tissues, which suggested Knockdown significantly inhibited survival...
Liver fibrosis is a major global health concern. Management of chronic liver disease severely restricted in clinics due to ineffective treatment approaches. However, lack targeted therapy may aggravate this condition. Asiatic acid (AA), pentacyclic triterpenoid acid, can effectively protect the from hepatic disorders. pharmaceutical application AA limited by low oral bioavailability and poor targeting efficiency. This study synthesized novel liver-targeting material PEG-SA, chemically linked...
Abstract Four new diorganotin(IV) complexes of N ‐(3,5‐dibromosalicylidene)tryptophane, R 2 Sn[3,5‐Br ‐2‐OC 6 H CHNCH(CH Ind)COO] [Ind = 3‐indolyl; Et (1); n ‐Bu (2); Cy (3); Ph (4)], were synthesized and characterized by elemental analysis IR, NMR ( 1 H, 13 C 119 Sn) spectra. The crystal structures 1–3 determined X‐ray single diffraction. tin atom is five‐coordinated its coordination geometry best described as an intermediate between trigonal bipyramidal square pyramidal. Intermolecular...
Brother of regulator imprinted sites (BORIS) is expressed in most cancers and often associated with short survival poor prognosis patients. BORIS inhibits apoptosis promotes proliferation cancer cells. However, its mechanism action has not been elucidated, there no known inhibitor BORIS.A phage display library was used to find the inhibitory peptides BTApep-TAT identified. The RNA sequencing profile BTApep-TAT-treated H1299 cells compared that BORIS-knockdown Antitumor activity evaluated a...
Abstract Four new diorganotin(IV) complexes of N ‐(5‐halosalicylidene)tryptophane, R 2 Sn[5‐X‐2‐OC 6 H 3 CHNCH(CH Ind)COO] [Ind = 3‐indolyl; R, X Et, Cl ( 1 ); Br( n ‐Bu, Br 4 )], were synthesized and characterized by elemental analysis, IR NMR H, 13 C 119 Sn) spectra. The crystal structures – determined X‐ray single diffraction showed that the tin atoms are in a distorted trigonal bipyramidal geometry form five‐ six‐membered chelate rings with tridentate ligand. Intermolecular weak...
Ubiquitin-specific peptidase 24 (USP24), a member of the deubiquitinase family, plays an important role in tumor regulation. However, USP24 Hepatocellular carcinoma（HCC）is unknown. The aim our study was to explore HCC seek new therapeutic targets for HCC. In this study, we found that aberrantly upregulated tissues and predicted poor prognosis. markedly promoted proliferation progression vitro vivo. Mechanistically, binds necrosis factor receptor-associated 2(TRAF2) inhibits its degradation,...
AZD9291 is a novel, irreversible epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI), which administered orally. It has been proven effective in non-small cell lung cancer (NSCLC) patients, with both EGFR-sensitizing and EGFR T790M mutations preclinical models. However, the potential therapeutic effects of combined other modalities, including ionizing radiation, are not well understood. The presence significantly increases cell-killing radiation PC-9-IR cells secondary...
In order to study the liver targeting of N-galactosylated chitosan (GC) polymer in liver, we first conjugated lactobionic acid with (CS) obtain carrier GC different degree substitution lactosyl group. Western blot was performed detect expression levels asialoglycoprotein receptors (ASGPR) cell lines HepG2, SMMC-7721, and HL-7702. The protein level ASGPR lower HepG2 compared HL-7702 SMMC-7721. Although all treated by CS, viabilities did not experience any significant drop, while viability...
Synaptopodin 2 (SYNPO2) plays a pivotal role in regulating tumor growth, development and progression bladder urothelial Carcinoma (BLCA). However, the precise biological functions mechanisms of SYNPO2 BLCA remain unclear. Based on TCGA database‑derived RNA sequencing data, survival analysis prognosis indicate that elevated expression was associated with poor outcomes. Notably, exogenous significantly promoted invasion migration by upregulating vimentin cell lines. Enrichment revealed...
Notch1 is a type-1 transmembrane receptor which has been demonstrated to be involved in proliferation various organisms. A number of studies have proposed that Notch signaling may aberrantly activated, thus contributing development, invasion and metastasis variety human cancers. In the present study, function mechanism prostate cancer (PCa) LNCaP cells vitro was investigated. cleaved-Notch1 expression were evaluated PCa cell lines, including LNCaP, PC-3 DU 145, epithelial RWPE-1 line....
5-Fluorouracil (5-FU) has been widely applied in treating cancers. However, its usage is largely limited hepatocellular carcinoma (HCC), due to acquired resistance. Here, we aim identify target proteins and investigate their roles 5-FU sensitivity of HCC cells. Mass spectrometry (MS) proteomics was performed on 5-FU-resistant cell line (BEL7402/5-FU) parental (BEL7402) with treatment. In order potential targets, compared the between two groups used bioinformatics tools select hub from all...
Abstract Two new dinuclear phenyltin(IV) complexes derived from N , ′‐ bis (2‐hydroxybenzyl)‐1,2‐ethane (dithiocarbamate) ligand, [2‐HOC 6 H 4 CH 2 N(CS SnPh 3 )CH ] ( 1 ) and SnClPh have been synthesized characterized by elemental analysis, IR NMR H, 13 C 119 Sn) spectra. The crystal structures of were determined X‐ray single diffraction show that the dithiocarbamate ligand is coordinated to tin atom in anisobidentate manner five‐coordinated. coordination geometry best described as an...
Merm1/Wbscr22 is a novel metastasis promoter that has been shown to be involved in tumor metastasis, viability and apoptosis. To the best of our knowledge, there are currently no studies suggesting possible correlation between expression cells chemosensitivity antitumor agents. In present study, two human non‑small cell lung cancer lines, H1299 H460, were used investigate whether affects agents, including cisplatin (CDDP), doxorubicin (ADM), paclitaxel (PTX), mitomycin (MMC),...