A5057254530- Cancer-related molecular mechanisms research
- RNA regulation and disease
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Chromatin Remodeling and Cancer
- Cancer Mechanisms and Therapy
- Reproductive biology and impacts on aquatic species
- Angiogenesis and VEGF in Cancer
- Glioma Diagnosis and Treatment
- DNA Repair Mechanisms
- Mycobacterium research and diagnosis
- Silymarin and Mushroom Poisoning
- Aquaculture Nutrition and Growth
- Developmental Biology and Gene Regulation
- Nutrition and Health in Aging
- Natural product bioactivities and synthesis
- Circular RNAs in diseases
- Invertebrate Immune Response Mechanisms
- Fungal Plant Pathogen Control
- Microtubule and mitosis dynamics
- Renin-Angiotensin System Studies
- Quinazolinone synthesis and applications
- Chemokine receptors and signaling
- Cancer Cells and Metastasis
Universidade de São Paulo
2016-2024
National Council for Scientific and Technological Development
2020-2021
Universidade Estadual Paulista (Unesp)
2021
Universidade Federal do Paraná
2015-2021
Clinics Hospital of Ribeirão Preto
2021
Massachusetts General Hospital
2018
Harvard University
2018
Universidade Federal de Minas Gerais
1996-2014
The University of Texas Health Science Center at San Antonio
2008-2010
Max Delbrück Center
2010
Abstract Most insects have a gut lined with peritrophic membrane (PM) consisting of chitin and proteins, mainly peritrophins that chitin‐binding domains. The PM is proposed to originate from mucus‐forming mucins (Mf‐mucins), which acquired domain interlocked chitin, replacing mucus in function. We evaluated the expression Mf‐mucins by RNA‐sequencing (RNA‐seq) throughout midgut four distantly related insects. were identified as proteins high o‐glycosylation series uninterrupted Pro/Thr/Ser...
Inflammation and angiogenesis are key components of fibrovascular tissue growth, a biological event underlying both physiological (wound healing) pathological conditions (tumor development, chronic inflammation). We investigated these in three frequently used mouse strains (Swiss, Balb/c C57BL/6J) to verify the influence genetic background on kinetics inflammatory cell recruitment/activation, neovascularization, extracellular matrix deposition, cytokine production polyether-polyurethane...
The transcription-associated cyclin dependent kinases (CDKs) 12 and 13 play important roles in the expression of RNAs involved DNA damage repair ribosomal proteins, yet their if any, regulation are unclear. Here we show that these CDKs critical processing degradation noncoding nucleolus inhibition function leads to upregulation unprocessed RNA (rRNA) transcripts intergenic spacer (IGS) regions between subunits. These upregulated aberrantly polyadenylated by TRAMP complex polymerase TENT4,...
// Lucas Goedert 1, 2, * , Cristiano G. Pereira Jason Roszik 3, Jessica R. Plaça 4 Cibele Cardoso 1 Guo Chen 3 Wanleng Deng Vashisht Gopal Yennu-Nanda Wilson A. Silva Jr. 5 Michael Davies Enilza M. Espreafico Department of Cell and Molecular Biology, Faculty Medicine Ribeirão Preto, University São Paulo, Brazil 2 National Institute Science Technology in Stem Therapy Center for Cell-Based Therapy, Melanoma Medical Oncology, The Texas MD Anderson Cancer Center, Houston,...
RMEL3 is a recently identified lncRNA associated with BRAFV600E mutation and melanoma cell survival. Here, we demonstrate strong moderate upregulation in BRAF NRAS mutant cells, respectively, compared to melanocytes. High expression also more frequent cutaneous than acral/mucosal melanomas, analysis of an ICGC dataset showed that mutations locus are preponderantly C > T substitutions at dipyrimidine sites including CC TT, typical UV signature. does not correlate levels, but poor patient...
This study aimed to evaluate the in vivo antitumor actions and toxicity of dichloromethane fraction (F1B) Moquiniastrum polymorphum subsp. floccosum (formerly Gochnatia polymorpha ssp. floccosa), composed sesquiterpene lactones, against Walker-256 carcinosarcoma rats.Male Wistar rats received 100 mg kg(-1) F1B per day orally for 16 days after subcutaneous inoculation cells pelvic limb. The tumor progression was monitored, treatment, weight, oxidative stress, plasma biochemistry, inflammatory...
The present study evaluated the effects of acute treatment with silymarin, an extract that is obtained from Silybum marianum, on angiogenesis, oxidative stress, and inflammation in normoglycemic diabetic mice. Diabetes was induced by streptozotocin (80 mg/kg, intraperitoneal) male Swiss mice, 6 weeks age. A polyether-polyurethane sponge surgically implanted back mice as a model healing both animals were treated oral silymarin or water for 10 days. pancreas, liver, kidneys, blood, sponges...
Stimulators of angiogenesis hold potential in promoting the development collateral circulation ischaemic tissue and accelerating wound healing, but promote pathological vasoformation angiogenesis-dependent diseases (solid tumours, atherosclerosis). The renin-angiotensin system is implicated both beneficial vascular growth. We investigated angiogenic activity angiotensin II (All) a sponge implant model mice; this peptide enhanced angiogenesis, as well glycosaminoglycan (GAG, chondroitin...
Injury of skeletal abdominal muscle wall is a common medical condition and implantation synthetic or biological material procedure to repair musculofascial defects. We proposed characterize the dynamics inflammatory cell recruitment, newly formed blood vessels, cytokine production fibrogenesis in response polyether-polyurethane sponge implants mice. At 2, 4, 7 10days after tissue underneath matrix was removed for assessment angiogenic (hemoglobin content, vascular endothelial growth factor...
This study evaluated the antitumour activity of mesoionic compound sydnone 1 (Syd-1) against Walker-256 carcinosarcoma. Tumour cells were subcutaneously inoculated in hind limb male Wistar rats. The animals orally treated for 12 days with Syd-1 (75 mg/kg) or vehicle. At end treatment, considerable decreases tumour volume and weight observed animals. Samples these tumours presented increases apoptotic bodies pro-apoptotic protein expression (Bax p53), while anti-apoptotic Bcl-2 was reduced. A...
Glioblastoma (GBM) is the most lethal and frequent type of brain tumor, leading patients to death in approximately 14 months after diagnosis. GBM treatment consists surgical removal followed by radio chemotherapy. However, tumors commonly relapse promotes only a slight increase patient survival. Thus, uncovering cellular mechanisms involved resistance utmost interest, use cell lines has been shown be an extremely important tool. In this work, exploration RNAseq data from different revealed...
Orthotopic cell transplantation models are important for a complete understanding of cell–cell interactions as well tumor biology. In published studies orthotopic in the mouse adrenal gland, human neuroblastoma cells have been shown to invade and occupy adrenal, but these investigations true model was not established. Here we show an which transplanted retained within gland by formation fibrin clot. To establish appropriate technique, used brightly fluorescent 10 μm polystyrene microspheres...
Summary Natriuretic peptide receptor‐C activation by the synthetic ligand C‐ ANP ‐ 4–23, a specific agonist for this receptor, has been shown to inhibit key events of angiogenic cascade, such as migration, proliferation and vascular endothelial growth factor ( VEGF ) production. In present study we investigated whether 4–23 could also angiogenesis in sponge model vivo . To end, evaluated effects on inflammatory components fibrovascular tissue induced polyether polyurethane implants mice....
Tonin is a serine proteinase of the kallikrein family that can produce angiotensin II directly from angiotensinogen. To clarify importance this enzyme for central nervous control cardiovascular system, we generated transgenic mice, TGM(rTon), express rat tonin in astrocytes. These mice present high levels mRNA and activity specifically brain. As consequence, TGM(rTon) develop increased blood pressure water intake. Lisinopril, an ACE inhibitor, less hypotensive than animals. The AT(1)...
Brain aromatase is a key enzyme exclusively expressed in fish radial glial cells that convert androgens into estrogens, thus controlling neuroendocrine functions and neurogenesis. As an important step characterizing the systems of Rhamdia quelen (jundiá), partial cDNA sequence (1045 bp) brain (cyp19a1b) was cloned sequenced. At nucleotide level found to be 88% identical cyp19a1b two species catfish, Ictalurus punctatus Silurus meridionalis. The predicted amino acid between 80 91% similar...
Expression of vitellogenin gene (vtg) is used as a biomarker for the evaluation exposure estrogenic substances in fish. However, scientific information regarding this Neotropical fish limited. In study, 760 bp partial sequence vtg mRNA from liver catfish Rhamdia quelen was cloned, representing almost 20% full-length vtg. The phylogenetic tree analysis recovered R. inside clade Siluriformes. alignment deduced amino acid with other species confirmed that described specific. Also, cloned...
BACKGROUND: Expression dysregulation of HOX homeobox genes has been observed in several cancers, including head and neck squamous cell carcinoma (HNSC). Although characterization gene roles HNSC development reported, there is still a need to better understand their real contribution tumorigenesis. OBJECTIVE: The present study aimed evaluate the protein-coding ( HOXA10, HOXC9, HOXC10, HOXC13) cellular processes related carcinogenesis progression HNSC. METHODS: was analyzed RNA-Seq data from...
Abstract PIMREG expression strongly correlates with cellular proliferation in both malignant and normal cells. Throughout embryo development, is prominent at the central nervous system. Recent studies have described high levels of transcripts different types tumors correlated patient survival tumor aggressiveness. Given emerging significance carcinogenesis its putative role context system, we investigated function gliomas, most common primary brain tumors. We performed an extensive analysis...