A5042197769- Renal Diseases and Glomerulopathies
- Monoclonal and Polyclonal Antibodies Research
- Reproductive Biology and Fertility
- Advanced Radiotherapy Techniques
- Reproductive System and Pregnancy
- Endometrial and Cervical Cancer Treatments
- IL-33, ST2, and ILC Pathways
- Inflammatory Bowel Disease
- Sperm and Testicular Function
- Biosimilars and Bioanalytical Methods
- Chronic Lymphocytic Leukemia Research
- Protein Degradation and Inhibitors
- Chronic Kidney Disease and Diabetes
- Breast Cancer Treatment Studies
- Urticaria and Related Conditions
- Prostate Cancer Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Amino Acid Enzymes and Metabolism
- Multiple Myeloma Research and Treatments
- Protein purification and stability
- Reproductive Physiology in Livestock
- Agriculture Sustainability and Environmental Impact
- Management of metastatic bone disease
- Lymphoma Diagnosis and Treatment
- Breast Implant and Reconstruction
Eli Lilly (United States)
2024-2025
Hokkaido University
2015-2024
Sapporo University
2024
Keio University
1995-2024
University of Edinburgh
2022
University of Pittsburgh
2022
Tokyo Metropolitan Children's Medical Center
2018-2021
Osaka University
2006-2020
Takarazuka City Hospital
2016-2020
Otsuka (Japan)
2020
Abstract The escalating threat of infections caused by drug-resistant bacteria poses a significant global health challenge, with projections estimating 10 million annual deaths 2050. While the development conventional antibiotics has stagnated since late 1990s, antimicrobial peptides (AMPs), short amino acid sequences exhibiting potent activity, have emerged as promising alternative, demonstrating efficacy even against bacteria. However, despite identification numerous AMPs, their...
Drug-resistant infections pose a global health challenge and necessitate the rapid development of novel antibiotics. Although high-speed high-accuracy in silico drug discovery methods using AI have been established, only few approaches that specifically target antibiotic developed. This gap significantly limits our ability to rapidly discover effective antibacterials against emerging resistant pathogens. Here, we developed BaCNet, an system accurately predicts binding affinity between...
Mirikizumab, a p19-directed antibody against interleukin-23 (IL-23), is administered by subcutaneous (SC) injection. Injection site pain (ISP) associated with citrate buffers may negatively affect patient adherence to SC-administered treatments. We assessed the bioequivalence and safety of citrate-free (CF) original formulations mirikizumab. The were in three phase 1, two-arm, randomized, single-dose, parallel design studies healthy participants: study A (NCT04548219), B (NCT05515601), C...
The purpose of this study is to report our clinical outcomes using intensity-modulated radiation therapy (IMRT) for adjuvant treatment cervical cancer, compared with three-dimensional conformal (3DCRT), in terms tumor control, complications and dose-volume histogram (DVH) parameters.Between March 2008 February 2014, 62 patients were treated concurrent nedaplatin-based chemotherapy whole-pelvic external beam (RT). Of these patients, 32 (52%) received 3DCRT 30 (48%) IMRT.The median follow-up...
Significant advancement of chemoproteomics has contributed to uncovering the mechanism action (MoA) small-molecule drugs by characterizing drug–protein interactions in living systems. However, cell-membrane proteins such as G protein-coupled receptors (GPCRs) and ion channels, due their low abundance unique biophysical properties associated with multiple transmembrane domains, can present challenges for proteome-wide mapping drug–receptor interactions. Herein, we describe development novel...
Significance Statement Tertiary lymphoid structures (TLSs) form in organs exhibiting chronic inflammation. Immune responses to infections, autoimmune responses, or allergic may trigger TLS development. We observed urine–urothelium barrier alterations the renal pelvis of a mouse model and human subjects with nephritis, without infection. Furthermore, urine leaked from lumen into parenchyma nephritic mice. This stimulated production cytokines/chemokines stromal cells, resulting development...
Toll-like receptors (Tlrs) are sensors of danger signals which promote the activation immune cells and intrinsic renal cells. Podocytes, glomerulus, continuously exposed to various plasma solutes due their unique location in glomerulus. Herein, we show that Tlr9 is overexpressed podocytes mechanisms cause its injury development membranoproliferative glomerulonephritis (MPGN) model BXSB/MpJ-Yaa (Yaa) mice. Yaa mice developed typical lesions MPGN showed strong expression mRNA throughout...
Abstract This study evaluated endothelial cells and podocytes, both being primary components of the glomerular filtration barrier, in progression membranoproliferative glomerulonephritis (MPGN) using modified scanning electron microscopy (mSEM) analysis. BXSB/MpJ- Yaa model mice exhibited autoimmune-mediated MPGN characterised by elevated serum autoantibody levels, albuminuria, renal dysfunctional parameters, decreased fenestrations (EF) podocyte foot process (PFP) effacement with immune...
The immune system is known to affect reproductive function, and maternal-fetal tolerance essential for a successful pregnancy. To investigate the relationship between autoimmune disease female we performed comparative analysis of ovarian phenotypes C57BL/6 mice, disease-prone MRL/MpJ (MRL/+) mice congenic MRL/MpJ-Fas(lpr) (MRL/lpr) harboring mutation in Fas gene that speeds onset. Both MRL-background strains showed earlier vaginal opening than mice. estrous cycle became irregular by 6 12...
Lupus nephritis (LN) is a common complication in young patients and the most predominant cause of glomerulonephritis. Infiltrating immune cells presence immunocomplexes kidney are hallmarks LN, which closely associated with renal lesions (RLs). However, their regulatory mechanism remains unclear, valuable for prevention RL development. Here, we show development vasculature-associated lymphoid tissue (VALT) related to inflammatory cytokines, indicating that VALT unique tertiary tissue....
The aim of this study was to assess the feasibility planning dose-volume histogram (DVH) parameters in computed tomography-based 3D image-guided brachytherapy for locally advanced cervical cancer. In a prospective multi-institutional study, 60 patients with stage IIA2-IVA cancer from eight institutions were treated external beam radiotherapy using central shielding and intracavitary or hybrid (combined intracavitary/interstitial) (HBT). dose constraints set as cumulative linear quadratic...
Gestational diabetes mellitus (GDM) in human patients disrupts glucose metabolism post-pregnancy, affecting fetal development. Although obesity and genetic factors increase GDM risk, a lack of suitable models impedes comprehensive understanding its pathology. To address this, we administered streptozotocin (STZ, 75 mg/kg) to C57BL/6N mice for two days before pregnancy, establishing convenient model. Pregnant exposed STZ (STZ-pregnant) were compared with STZ-injected virgin (STZ-virgin),...
Riboflavin (vitamin B2) plays a role in various biochemical oxidation-reduction reactions. Flavin mononucleotide (FMN) and FAD, the biologically active forms, are made from riboflavin. transporters (RFVTs), RFVT1-3/Slc52a1-3, have been identified. However, roles of human (h)RFVTs FMN FAD homeostasis not yet fully clarified. In this study, we assessed contribution each hRFVT to riboflavin, uptake efflux using vitro studies. The transfection hRFVTs increased cellular riboflavin concentrations....
In Japan, breast-conserving surgery with closed cavity has generally been performed for breast cancer patients, and accelerated partial irradiation (APBI) is considered difficult because Asian females have smaller sizes than Western females. Therefore, common identification of target treatment plan method in APBI required. A prospective multicenter study was conducted Japan to determine institutional compliance using high-dose-rate interstitial brachytherapy (ISBT) designed Japanese female...
2.3-month-old (Case 1), one-month-old 2) and 6-month-old 3), Japanese Black calves presented with mild to severe wheezing. All had histories of dystocia at birth breech presentation. Physical examination, thoracic radiography, endoscopy or computed tomography indicated wheezing associated tracheal collapse stenosis caused by perinatal rib fractures. Partial resection the fractured first second ribs was performed on all calves. The respiration in Cases 1 2 immediately improved after surgery,...
We investigated spatiotemporal changes in host tissues during foreign body reactions. Silicone tube was subcutaneously embedded into ICR mice, and tissue surrounding silicone (TSS) observed at 2, 7, 14, 21, 28, 43, 70 days (D) postsurgery. The thin layer (TL) loose connective (LCTs) (inside outside the TSS) developed until D21 densified afterward. Neutrophils infiltrated TSS D14 formed neutrophil extracellular traps (NETs) TL D7-21. In LCTs, mast cell counts increased D21, macrophage numbers...
Between August 15th and 19th, 2011, eight 5th-year medical students from the Keio University School of Medicine had opportunity to visit Peking hold a discussion session titled “What is most effective way educate people for survival in an acute disaster situation (before mental health care stage)?” During session, we discussed following six points: basic information regarding Sichuan Earthquake East Japan Earthquake, differences preparedness earthquakes, government actions, acceptance rescue...
PhiC31 integrase has the potential to achieve long-term transgene expression because of site-specific and unidirectional recombination. In this study, plasmid DNA (pDNA) encoding Gaussia luciferase (Gluc) cDNA was constructed optimal condition for gene using phiC31 in mouse liver after hydrodynamic injection investigated. Gluc is secreted thus allows quantification its level blood easy determination time-course. Mice were co-transfected with 25 µg donor pDNA (pORF-Gluc/attB) different...
Objective: To evaluate the changing trends of standards and practices for postoperative radiotherapy (RT) patients with prostate cancer in Japan.