A5059603836- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Immune Cell Function and Interaction
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Occupational and environmental lung diseases
- Hippo pathway signaling and YAP/TAZ
- Occupational exposure and asthma
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Atherosclerosis and Cardiovascular Diseases
- Colorectal Cancer Treatments and Studies
- Metastasis and carcinoma case studies
- Medicinal Plant Pharmacodynamics Research
- Monoclonal and Polyclonal Antibodies Research
- Genital Health and Disease
- Pulmonary Hypertension Research and Treatments
- COVID-19 and healthcare impacts
- Ferroptosis and cancer prognosis
- Liver physiology and pathology
- Single-cell and spatial transcriptomics
- Neonatal Respiratory Health Research
Jiangsu Province Hospital
2021-2025
Nanjing Medical University
2017-2025
Second Affiliated Hospital of Nanjing Medical University
2018-2021
National Health and Family Planning Commission
2019
Immune functions decline with aging, leading to increased susceptibility various diseases including tumors. Exploring aging-related molecular targets in elderly patients cancer is thus highly sought after. Here we find that an ER transmembrane enzyme, sterol O-acyltransferase 2 (SOAT2), overexpressed regulatory T (Treg) cells from lung squamous cell carcinoma (LSCC), while radiomics analysis of LSCC associates SOAT2 expression reduced immune infiltration and poor prognosis. Mechanically, ex...
Gastric cancer is one of the most common types human cancer, and it additionally leading causes cancer‑associated mortality worldwide. Previous studies have suggested that interleukin (IL)‑10 may contribute to pathogenesis gastric cancer. However, underlying mechanisms remain unclear. In present study, was observed expression IL‑10 significantly upregulated in tumor tissues serum samples patients with Furthermore, increased cell culture supernatant macrophages (CAMs). Treatment from CAMs...
Cholangiocarcinoma (CCA) is the most common biliary tract malignancy, with a low survival rate and limited treatment options. Long non-coding RNAs (lncRNAs) have recently been verified to significant regulatory functions in many kinds of human cancers. It was discovered this study that lncRNA PVT1, whose expression significantly elevated CCA, could be molecular marker CCA. Experiments indicated PVT1 knockdown greatly inhibited cell migration proliferation vitro vivo. According RNA sequencing...
Recently, long noncoding RNAs (lncRNAs) have been shown to play significant regulatory roles in human tumorigenesis. However, the biological function of lncRNAs cholangiocarcinoma (CCA) remains largely unknown. In this study, DANCR was be significantly upregulated CCA. regulated proliferation and migration CCA cells vitro. Moreover, downregulation suppressed vivo. RNA-seq revealed that knockdown preferentially affected genes linked with cell differentiation. Furthermore, mechanistic...
Abstract Cholangiocarcinoma (CCA) is the as most frequently observed biliary tract malignancy, which has low survival rate in addition to constrained treatment options; nevertheless, fundamental molecular phenomenon underlying malignant progression of CCA quite ambiguous. Recently long non-coding RNAs (lncRNAs) have been found significant regulatory functions several human cancers. Herein, we figured out that lncRNA SNHG1 , with substantially enhanced expression CCA, capable acting oncogenic...
Abstract Background Autophagy is a double-edged sword during the initiation and progression of multiple tumors. The Hippo pathway effector YAP has been proved to be involved in autophagy processes. present study aimed investigate how regulates cell proliferation via lung adenocarcinomas (LUAD). Methods Data LUAD chip GSE43458 was obtained from Gene Expression Omnibus (GEO). RT-qPCR Western blot were performed assess expression lines. CCK-8 assay, xenograft tumor model, immunochemistry...
Hepatocellular carcinoma (HCC) is one of the most mortality-causing solid cancers globally and second largest cause death among malignancies. Oxaliplatin, a platinum-based drug, has been widely utilized in treatment malignancies such as colorectal cancer hepatocellular carcinoma, yet its usage limited because severe side effects cytotoxicity to normal tissues. c-Met, receptor tyrosine kinase, expressed aberrantly on surface carcinoma. The purpose this study was synthesize humanized antibody...
Background: Nicotinamide mononucleotide (NMN) is the physiological circulating NAD precursor thought to elevate cellular level of NAD+ and ameliorate various age-related diseases. An inseparable link exists between aging tumorigenesis, especially involving aberrant energetic metabolism cell fate regulation in cancer cells. However, few studies have directly investigated effects NMN on another major ageing-related disease: tumors. Methods: We conducted a series mouse models evaluate...
Background/Aim: The prevalence of idiopathic pulmonary fibrosis (IPF) increases with age and is associated senescence alveolar epithelial cells (AECs). AEC in mediates IPF. We herein aimed to determine if YAP1 gene knockdown, a member the Hippo/YAP signal pathway, bleomycin (BLM)-induced mouse model IPF, inhibits onset AECs alleviates Materials Methods: Adeno-associated viruses (AAVs) expressing Yes-associated protein 1 (YAP1) short hairpin RNA (shRNA) were delivered into lung BLM-induced...
Objectives In a genome-wide association study, we discovered chromosome 12q15 (defined as rs73329476) silica-related pneumoconiosis susceptibility region. However, the causal variants in this region have not yet been reported. Methods We systematically screened eight potentially functional single-neucleotide polymorphism (SNPs) genes near rs73329476 (carboxypeptidase M ( CPM ) and cleavage polyadenylation specific factor 6 CPSF6 )) case–control study including 177 cases with silicosis 204...
ABSTRACT Background and objective This study aimed to identify miRNA as potential diagnostic biomarkers for silica‐related pulmonary fibrosis (SPF). Methods We first performed a comprehensive miRNA‐seq screening in PBL of eight subjects exposed silica dust (four individuals with SPF four healthy controls). The promising were then evaluated the first‐stage validation using an independent GEO data set (GSE80555) 6 (3 3 controls), followed by second‐stage 120 (60 60 Results Thirty‐five showed...
BACKGROUND:Chemotherapy is widely used in gastric cancer treatment, but multidrug resistance remains a leading cause of chemotherapy failure. Trop2 highly expressed tumor tissues and greatly influences progression. However, little known about the relationship between drug cancer. MATERIAL AND METHODS:In present study, was knocked down BGC823 cells overexpressed HGC27. CCK-8 assay performed to explore expression cell proliferation treated with anticancer drugs. Flow cytometry assess apoptosis...
Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal interstitial pneumonia disease with no cure. Communication between injured cells triggered maintained by complicated network of cytokines their receptors. IL-19 supported increasing evidences for deleterious role in respiratory diseases. However, its potential lung has never been explored.Bioinformatic, immunohistochemistry western blot analysis were used to assess the expression human mouse tissues. CCK-8, transwell flow...
Abstract To investigate the associations between functional single nucleotide polymorphisms (SNPs) in miR‐125 family and survival of non‐small cell lung cancer (NSCLC) patients, we systematically selected six SNPs located three pre‐miRNAs (miR‐125a, miR‐125b‐1, miR‐125b‐2). Cox proportional hazard regression analyses were conducted to estimate crude adjusted ratios (HRs) their 95% confidence intervals (CIs). Reporter gene luciferase assay was performed examine relationship transcriptive...
Background: As reported by numerous research studies, the expression levels of SNHG1 (small nucleolar RNA host gene 1) are increased in different kinds tumours, revealing that is likely to perform a crucial function cancer prevalence and progression. Moreover, mounting degree evidence suggests also has an association with poor medical outcomes among patients. Materials methods: Collection qualifying studies was performed through retrieval keywords PubMed Web Science, up March 20, 2018. This...
Abstract Background: Autophagy is a double-edged sword during the initiation or progression of multiple tumors. Hippo pathway core-factor YAP has been proved to be involved in autophagy processes. The present study aimed identify mechanism underlying modulation via PTEN/AKT/mTOR-mediated lung adenocarcinomas (LUAD).Methods: Data LUAD chip GSE43458 was obtained from Gene Expression Omnibus (GEO). RT-qPCR and Western blot were used assess expression cell lines. CCK-8 assay, flow cytometry,...
In December 2019, there was an outbreak of pneumonia unknown causes in Wuhan, China. The etiological pathogen identified to be a novel coronavirus, named severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), which disease 2019 (COVID-19). number infected patients has markedly increased since the and COVID-19 also proven highly contagious. particular, elderly are among group who most susceptible succumbing within general population. Cross-infection hospital is one important route...
Alzheimer's Syndrome (AD) is a neurodegenerative disease that prevalent in middle-aged and elderly people. As the progresses, patients gradually lose ability to take care of themselves, which brings heavy burden family. There link between leukocyte telomere length (LTL) cognitive ability. To search for possible pathogenic mechanisms potential therapeutic agents, we demonstrated causal LTL AD using Mendelian randomization analysis (MR). The expression target gene NBR2 downstream mRNA GJA1...
Lung cancer is the most prevalent and lethal disease globally, with approximately 80% of cases being non-small cell lung (NSCLC). NSCLC primarily composed squamous carcinoma (LUSC) adenocarcinoma (LUAD). Despite chemotherapy currently primary treatment for NSCLC, resistance remains a significant challenge patients. Recent studies have proposed immunotherapy as promising new avenue treating NSCLC. The association between lysyl oxidase-like 2 (LOXL2) gene was explored using multiple online...
Objective: To produce latent membrane protein 2A (LMP2A) chimeric antigen receptor (CAR)-T cells and detect the lethal effect of LMP2A CAR-T on nasopharyngeal carcinoma (NPC) cells. Methods: The study was conducted from September 2016 to December 2017.Genetic engineering technology used construct anti-LMP2A CAR lentiviral expression vector sequencing identified. in 293T confirmed by western blot. CCK8 assay evaluate cytotoxicity NPC ELISA performed test IL-2 IFN-γ releasing activated...
Lung cancer is one of the most prevalent cancers and leading cause cancer-related deaths worldwide; non-small cell lung (NSCLC) comprises approximately 80% all cases. This study aimed to construct a competing endogenous RNA (ceRNA) network identify prognostic signatures in elderly patients with NSCLC.We extracted data from NSCLC The Cancer Genome Atlas identified differentially expressed (DE) messenger RNAs (mRNAs), microRNAs (miRNAs), long non-coding (lncRNAs). Gene Ontology Kyoto...
High Trop2 expression relates to aggressive tumor behavior and contributes poor overall survival rates in gastric cancer (GC) patients. However, little is known about the molecular mechanism of carcinogenesis GC. We found that over-expressed induced cell proliferation clone formation, inhibited apoptosis S cycle arrest GC lines, meanwhile, knockdown inhibits vitro. Moreover, depletion growth , anti-tumor rate this report being 22.53% vivo. In addition, activated PI3K/Akt signaling pathway...