A5101996211- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Microbial Metabolic Engineering and Bioproduction
- Cancer Immunotherapy and Biomarkers
- Reproductive Biology and Fertility
- Peptidase Inhibition and Analysis
- HVDC Systems and Fault Protection
- Biochemical Acid Research Studies
- Birth, Development, and Health
- Assisted Reproductive Technology and Twin Pregnancy
- Pluripotent Stem Cells Research
- Hippo pathway signaling and YAP/TAZ
- Climate Change and Health Impacts
- Cancer, Hypoxia, and Metabolism
- Amino Acid Enzymes and Metabolism
- Air Quality and Health Impacts
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Biofuel production and bioconversion
- Zebrafish Biomedical Research Applications
- Enzyme Catalysis and Immobilization
- Silk-based biomaterials and applications
- Global Health Care Issues
- Retinoids in leukemia and cellular processes
- Renal and related cancers
Shanghai First Maternity and Infant Hospital
2021-2025
Nanjing Tech University
2019-2023
Australian Regenerative Medicine Institute
2023
Tongji University
2020-2023
Monash University
2023
Southern Medical University
2022
Nanfang Hospital
2022
Affiliated Hospital of Qingdao University
2017-2021
Qingdao University
2017-2021
Second Affiliated Hospital of Zhejiang University
2020
Immune functions decline with aging, leading to increased susceptibility various diseases including tumors. Exploring aging-related molecular targets in elderly patients cancer is thus highly sought after. Here we find that an ER transmembrane enzyme, sterol O-acyltransferase 2 (SOAT2), overexpressed regulatory T (Treg) cells from lung squamous cell carcinoma (LSCC), while radiomics analysis of LSCC associates SOAT2 expression reduced immune infiltration and poor prognosis. Mechanically, ex...
The effects of ambient air pollutants on adverse pregnancy outcomes have been reported. However, studies about exposure and in patients undergoing IVF were limited inconclusive. To date Shanghai has the only city China to implement a compulsory single embryo transfer policy for all their first procedure effective from January 2019. We aimed investigate associations between biochemical live births, identify potential vulnerability characteristics Shanghai, China. A retrospective cohort study...
Serine/arginine-rich splicing factor 9 (SRSF9) is a classical RNA-binding protein that essential for regulating gene expression programs through its interaction with target RNA. Whether SRSF9 plays an role in colorectal cancer (CRC) progression and can serve as therapeutic largely unknown. Here, we highlight new findings on the of CRC elucidate underlying mechanism.CRC cell lines clinical tissue samples were used. qRT-PCR, Western blotting, immunohistochemistry (IHC), gain- loss-of-function...
Background Excessive exposure to PM2.5 can be detrimental reproductive health. The objective of this study was investigate the potential associations between ambient during different periods and negative pregnancy outcomes, such as miscarriage preterm birth, in patients who underwent assisted technology (ART). Methods This retrospective cohort examined outcomes 2,839 infertile women aged ≤ 45 years their first fresh or frozen-thawed embryo transfer at Shanghai First Maternity Infant Hospital...
Abstract Background Autophagy is a double-edged sword during the initiation and progression of multiple tumors. The Hippo pathway effector YAP has been proved to be involved in autophagy processes. present study aimed investigate how regulates cell proliferation via lung adenocarcinomas (LUAD). Methods Data LUAD chip GSE43458 was obtained from Gene Expression Omnibus (GEO). RT-qPCR Western blot were performed assess expression lines. CCK-8 assay, xenograft tumor model, immunochemistry...
Abstract Long noncoding RNAs (lncRNAs) have recently been verified to significant regulatory functions in many types of human cancers. The lncRNA ANRIL is transcribed from the INK4b‐ARF‐INK4a gene cluster opposite direction. Whether can act as an oncogenic molecule cholangiocarcinoma (CCA) remains unknown. Our data show that knockdown greatly inhibited CCA cell proliferation and migration vitro vivo. According results RNA sequencing analysis, dramatically altered target genes associated with...
Increasing evidence suggests that in vitro fertilization (IVF) may be associated with an increased risk of developing obesity and metabolic diseases later life the offspring. Notably, addition melatonin to culture medium improve embryo development prevent cardiovascular dysfunction IVF adult mice. This study aimed determine if supplementation can reverse impaired glucose metabolism mice offspring underlying mechanisms. Blastocysts used for transfer were generated by natural mating (control...
L-Tryptophan (L-Trp) derivatives such as 5-hydroxytryptophan (5-HTP) and 5-hydroxytryptamine (5-HT), N-Acetyl-5-hydroxytryptamine melatonin are important molecules with pharmaceutical interest. Among, 5-HT is an inhibitory neurotransmitter proven benefits for treating the symptoms of depression. At present, depends on plant extraction chemical synthesis, which limits its mass production causes environmental problems. Therefore, it necessary to develop efficient, green sustainable...
Background: Nicotinamide mononucleotide (NMN) is the physiological circulating NAD precursor thought to elevate cellular level of NAD+ and ameliorate various age-related diseases. An inseparable link exists between aging tumorigenesis, especially involving aberrant energetic metabolism cell fate regulation in cancer cells. However, few studies have directly investigated effects NMN on another major ageing-related disease: tumors. Methods: We conducted a series mouse models evaluate...
The establishment of ungulate embryonic stem cells (ESCs) has been notoriously difficult via a conventional approach. We combined traditional ESC culture method with reprogramming factors to assist the porcine naive-like ESCs (nESCs). Pig fibroblasts were transfected tetracycline-inducible vector carrying 4 classic mouse factors, followed by somatic cell nuclear transfer and culturing blastocyst stage. Then, inner mass was isolated seeded in medium. had characteristic verys similar those...
Abstract SIX1 and SIX4 genes play critical roles in kidney development. We evaluated the effect of these on pig development by generating −/− / foetuses using CRISPR/Cas9 somatic cell nuclear transfer. obtained 3 16 at different developmental stages. The showed a migration block left smaller size for both kidneys. ureteric bud failed to form normal branching collecting system. Abnormal expressions development‐related (downregulation PAX2, PAX8, BMP4 upregulation EYA1 SALL1) were also...
D-1,2,4-Butanetriol (BT) has attracted much attention for its various applications in energetic materials and the pharmaceutical industry. Here, a synthetic pathway biosynthesis of BT from d-arabinose was constructed optimized Escherichia coli. First, E. coli Trans1-T1 selected synthesis BT. Considering different performance enzymes organisms when expressed coli, optimized. After screening two dehydrogenases (ARAs), d-arabinonate dehydratases (ADs), four 2-keto acid decarboxylases (ADXs),...
Abstract Background Hepatocellular carcinoma (HCC) is one of the most common and malignant tumors in digestive tract. Tumor Suppressor Candidate 3 (TUSC3) subunit endoplasmic reticulum Oligosaccharyl transferase (OST) complex, which plays an important role N-glycosylation during protein folding process. However, TUSC3 initiation progression HCC has not been mentioned yet. In present study, we aim to investigate effects on HCC. Methods Immunohistochemical assay qRT-PCR were used detect...
The present study was designed to investigate the chemical constituents of Lindera nacusua and their antitumor activities. A new phenolic glycoside, namely 1-O-3-hydroxyphenyl-5-methoxyphenol-(6'-O-vanilloyl)-β-d-glucopyranoside (1), together with five known glycosides (2-6), two anthraquinones (7, 8) γ-butanolides (9, 10), isolated, its structure elucidated by spectroscopic methods. Compounds 1-10 were screened for in vitro cytotoxicities against HL-60, SMMC-7721, A549, MCF-3 SW480 cell...
Abstract Background: Autophagy is a double-edged sword during the initiation or progression of multiple tumors. Hippo pathway core-factor YAP has been proved to be involved in autophagy processes. The present study aimed identify mechanism underlying modulation via PTEN/AKT/mTOR-mediated lung adenocarcinomas (LUAD).Methods: Data LUAD chip GSE43458 was obtained from Gene Expression Omnibus (GEO). RT-qPCR and Western blot were used assess expression cell lines. CCK-8 assay, flow cytometry,...
Embryo vitrification is a standard procedure in assisted reproductive technology. Previous studies have shown that frozen embryo transfer associated with an elevated risk of adverse maternal and neonatal outcomes. This study aimed to explore the effects mouse blastocyst on phenotype vitrified-warmed blastocysts, their intrauterine postnatal development, long-term metabolic health derived offspring. The blastocysts (IVF + VT group) exhibited reduced mitochondrial activity, increased apoptotic...
T-cell-based immunotherapy and immune checkpoint blockade have been successfully used to treat several human solid cancers. The present study attempted investigate the feasibility efficacy of antitumor effect adoptive cell therapy along with programmed death protein 1 (PD-1) inhibitor on triple-negative breast cancer (TNBC). Tumor infiltration lymphocytes (TILs) from TNBC mouse tumor tissues were isolated expanded, TILs for (TILs-ACT) applied in combination a PD-1 model. pre- post-therapy...
This retrospective cohort study aimed to compare clinical outcomes following fresh or frozen embryo transfer (FET) in women with advanced reproductive age (ARA). Women aged 35–45 years who underwent their first autologous cleavage stage cycle the Centre for Assisted Reproduction of Shanghai First Maternity and Infant Hospital between January 2016 December 2020 were included. The primary outcome was live birth after vitro fertilization (IVF) cycle. Multiple covariates used propensity score...
Abstract T-cell-based immunotherapy and immune checkpoint blockade have been successfully used to treat several human solid cancers. The present study attempted investigate the feasibility efficacy of antitumour effect adoptive cell therapy along with programmed death protein 1 (PD-1) inhibitor on triple-negative breast cancer (TNBC). We isolated expanded tumour infiltration lymphocytes (TILs) from TNBC mouse tissues TIL transfusion (TILs-ACT) was applied in combination a PD-1 model. pre-...
Background and Aims: Embryo vitrification is widely applied as a routine procedure of assisted reproductive technology. Cohort studies showed frozen embryo transfer (FET) was associated with an elevated risk adverse maternal neonatal outcomes compared fresh transfer. This study aimed to explore the effects blastocyst on development long-term metabolic health in mice. Method: Mitochondrial function, apoptotic levels cell numbers were examined blastocysts with/without vitrification....
Objective To investigate the effect of paired related homoeobox 1 (Prrx1) on epithelial-mesenchymal transition (EMT) and migration ability breast cancer cells. Methods Breast cell lines (including BT-549, MDA-MB-468 MCF-7) were screened by real-time quantitative polymerase chain reaction (Real-time PCR) to obtain cells with lowest expression Prrx1. Then transfected Prrx1 over-expression letiviral vectors. The morphologic changes observed after transfection was verified be accomplished...