A5100721405- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Diabetes and associated disorders
- Asthma and respiratory diseases
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Diabetes Management and Research
- Cytomegalovirus and herpesvirus research
- Adenosine and Purinergic Signaling
- Pancreatic function and diabetes
- Heat shock proteins research
- Pediatric health and respiratory diseases
- Animal Virus Infections Studies
- Allergic Rhinitis and Sensitization
- Atherosclerosis and Cardiovascular Diseases
- Reproductive System and Pregnancy
- Mesenchymal stem cell research
- Pharmacological Effects of Natural Compounds
- Gout, Hyperuricemia, Uric Acid
- Sinusitis and nasal conditions
- thermodynamics and calorimetric analyses
- Virus-based gene therapy research
- Oral and gingival health research
- Ultrasound in Clinical Applications
- Hemodynamic Monitoring and Therapy
Ministry of Agriculture and Rural Affairs
2025
Poultry Research Institute
2025
Key Laboratory of Guangdong Province
2025
Guangdong Academy of Agricultural Sciences
2025
Guang’anmen Hospital
2024
Capital Medical University
2009-2023
Beijing Ditan Hospital
2018-2019
Beijing Tongren Hospital
2008-2018
Beijing Institute of Neurosurgery
2018
China Pharmaceutical University
2009-2014
Diabetes mellitus type 1 (DM1) is an autoimmune disease that gradually destroys insulin-producing beta-cells. We have previously reported mucosal administration of fusion protein HSP65 with tandem repeats P277 (HSP65-6P277) can reduce the onset DM1 in non-obese diabetic (NOD) mice. To deliver large amounts and to enhance long-term immune tolerance effects, present study, we investigated efficacy using orally administrated L. lactis expressing HSP65-6P277 incidence NOD strain NZ9000 was...
The important pathophysiological role of immune dysfunction, especially innate dysfunction in patients with acute-on-chronic liver failure (ACLF), has been investigated recent years, but dysregulation adaptive immunity remains poorly elucidated. aim this study was to (i) determine the CD3(+) T-lymphocyte count and balance between CD4(+) regulatory T (Tregs) conventional cells (Tconv) hepatitis B virus (HBV)-related ACLF patients; (ii) analyse frequencies Tregs subpopulations; (iii) assess...
Background The role of nasal nitric oxide (NO) in the diagnosis allergic rhinitis (AR) is controversial. aim this study was to identify factors that may affect levels NO AR patients and evaluate AR. Methods Seventy‐five 31 healthy controls were enrolled study. symptom scores assessed using visual analog scale. Eosinophil cationic protein (ECP) detected by enzyme‐linked immunoassay, measured a chemiluminescence analyzer, airway resistance (NAR) active anterior rhinomanometry. Results Nasal...
Mycophenolic acid (MPA) is the pharmacologically active compound of immunosuppressive drug mycophenolate mofetil. Dendritic cells (DC) are key to initiating and priming adaptive immune response leading allograft rejection. We investigated effects MPA on human blood myeloid DC (MDC) homeostasis with a particular emphasis their trafficking properties.Isolated peripheral mononuclear from healthy donors were cultured for 48 hr without addition exogenous growth factors. Flow cytometry was used...
Accumulating evidence established a positive association of anti-heat shock protein 60 (HSP60) autoantibodies and the presence atherosclerosis. However, whether anti-P277 (HSP60 437-460) may lead to pathological increase in vascular permeability, leak syndrome (VLS), is unknown. In present study, immunity was effectively induced C57BL/6 mice, causing marked VLS both normal mice those bearing melanoma as well. Further analysis role revealed that B-cell epitopes located P277 played causal...
Background Bone marrow derived mesenchymal stem cells (BMdMSCs) can differentiate into cardiomyocyte-like induced by different inductors individually or collectively. In this study, inducing BMdMSCs with p53 inhibitor (p-fifty three inhibitor-alpha, PFT-α), 5-azacytidine (5-AZA), angiotensin-II (Ang-II) and bone morphogenic protein-2 (BMP-2) we compared the influences of four on differentiation rat caridomyocyte like-cells. Methods were collected from Sprague Dawley rats after fourth...
Interferon-α (IFN-α) is widely used for the treatment of malignant and viral diseases. Conflicting results IFN-α–mediated effects on dendritic cell (DC) homeostasis have been reported its impact human blood DC largely unknown. We investigated phenotypic, migratory, allostimulatory activities plasmacytoid DCs (PDCs) myeloid (MDCs) upon in vitro exposure to IFN-α without addition exogenous growth factors. IFN-α–exposed PDCs exhibited an increase viability but showed immature phenotype a...
Building embodied agents on integrating Large Language Models (LLMs) and Reinforcement Learning (RL) have revolutionized human-AI interaction: researchers can now leverage language instructions to plan decision-making for open-ended tasks. However, existing research faces challenges in meeting the requirement of open-endedness. They typically either train LLM/RL models adapt a fixed counterpart, limiting exploration novel skills hindering efficacy interaction. To this end, we present...
Abstract Cardiomyocyte pyroptosis is a primary contributor to sepsis-induced cardiac dysfunction (SICD). Recombinant phospholipid transfer protein (PLTP) have been demonstrated possess anti-inflammatory and antiseptic properties. However, the effect of PLTP on SICD remains unknown. In this study, we established in vivo vitro sepsis model with recombinant treatment. The survival rates mice, mouse function, cell viability, level proinflammatory cytokine, lactate dehydrogenase were evaluated....
The association between heat shock protein (HSP) 65 and immune diseases has been investigated for many years. aim of this study was to explore the antitumor effects possible mechanism HSP65. Mice were immunized with HSP65 via subcutaneous injection. Specific IgG antibodies against detected in sera mice by enzyme‑linked immunosorbent assay verified western blot analysis. effectively inhibited growth tumors as well both protective therapeutic immunities melanoma tumor models prolonged survival...
Objective To study the janus kinase-signal transducer and activator of transcription 3 ( STAT3 ) inhibitor (Piceatannol) on growth hepatocellular carcinoma (HCC) in vitro.Methods The present created four groups: control group,primary human HCC cell lines HepG2,Huh7 );study group,control group + Piceatannol; negative group,human cervical line (Hela); positive group,Hela interferon (IFN) -α.The protein levels phosphorylated were detected by Western blotting.The cycle rate apoptotic cells...
To approach the evaluative effect of respiratory variation superior vena cava peak flow velocity measured using transthoracic echocardiography (TTE) on fluid responsiveness in patients with mechanical ventilation.A prospective cohort study was conducted. All ventilated critically ill whose therapy planned due to hypovolemia Department Critical Care Medicine Beijing Tongren Hospital Capital Medical University from April 2011 2013 were enrolled. Volume expansion performed 500 mL Linger...
<title>Abstract</title> Introduction The impact of eosinophil cationic protein (ECP) on nasal epithelial cells remains unclear. Our study investigated whether ECP could activate to secrete interleukin 8 (IL-8). Materials and Methods airway cells, NCI-H292, were cultured separately exposed varying concentrations for 2, 4, 6, 16, or 24 hours. supernatant was collected IL-8 tected using ELISA. Results results showed a significant increase in levels the group compared control at hours...
Objective To investigate the effect of intensive insulin therapy in critically ill elderly patients.Methods Elderly patients ( ≥ 65 years) admitted to ICU Beijing Tongren Hospital from June 2005 December 2007 were divided into Group A glucose control target was 4.4-6.1mmol/L) and B 7.3-8.3mmol/L).Blood level controlled with a computer-assisted protocoL Results total 639 enrolled,of which 280 359 B.The mean blood 2 groups (6.07 ± 0.56) mmol/L (7.52 0.87 ) respectively,both within ranges.The...
Objective To study the effect of mycophenolic acid(MPA)on maturation, endocytotic capacity and allostimulatory properties human myeloid dendritic cell ( MDC ). Methods The peripheral blood mononuclear cells were isolated freshly from healthy volunteers (n = 15). group was treated by MPA. Co-stimulatory adhesion molecular on analyzed flow cytometry. After isolation antigen-1 + BDCA-1 ), cellular uptake FITC-dextran measured mixed lymphocyte reaction, percentage allogenic CD+4 T in G0 phase...