A5049091862- Liver physiology and pathology
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Phagocytosis and Immune Regulation
- Circular RNAs in diseases
- Genetic and Kidney Cyst Diseases
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Liver Disease Diagnosis and Treatment
- Microtubule and mitosis dynamics
- Immune cells in cancer
- Erythrocyte Function and Pathophysiology
- MicroRNA in disease regulation
- Neuropeptides and Animal Physiology
- Antimicrobial Peptides and Activities
- Cancer Genomics and Diagnostics
- Endoplasmic Reticulum Stress and Disease
- Cell Adhesion Molecules Research
- Nuclear Receptors and Signaling
- Hydrogen's biological and therapeutic effects
- Adrenal Hormones and Disorders
- Obesity, Physical Activity, Diet
- Autophagy in Disease and Therapy
- Macrophage Migration Inhibitory Factor
- Single-cell and spatial transcriptomics
Montreal Clinical Research Institute
2016-2024
Army Medical University
2021
Hubei Engineering University
2020
VIB-KU Leuven Center for Cancer Biology
2019
KU Leuven
2019
Huzhou University
2015-2018
Zhongshan Hospital
2018
Fudan University
2006-2018
Affiliated Hospital of Hangzhou Normal University
2015
Shanghai Medical College of Fudan University
2007-2013
Hepatic stellate cells (HSCs) activation is an initial event in liver fibrosis. MicroRNAs (miRNAs) have been found to play essential roles cell differentiation, proliferation, and fat metabolism. In this study, we showed that down‐regulation of two over‐expressed miRNAs, miR‐27a 27b allowed culture‐activated rat HSCs switch a more quiescent HSC phenotype, with restored cytoplasmic lipid droplets decreased proliferation. Mechanistically, retinoid X receptor α was confirmed be the target 27b....
Liver coordinates a series of metabolic adaptations to maintain systemic energy balance and provide adequate nutrients for critical organs, tissues cells during starvation. However, the mediator(s) implicated in orchestrating these fasting-induced adaptive responses underlying molecular mechanisms are still obscure. Here we show that hepatic growth differentiation factor 15 (GDF15) is regulated by IRE1α-XBP1s branch promotes fatty acids β-oxidation ketogenesis upon fasting. GDF15 expression...
Blockade of the inhibitory checkpoint SIRPα-CD47 promotes phagocytosis cancer cells by macrophages and is a promising avenue in anti-cancer therapy. Productive strictly predicated on co-engagement pro-phagocytic receptors—namely, Fc receptors (FcRs), integrin CD11b, or SLAMF7—by their ligands cells. Here, we examine whether additional could be harnessed to broaden scope phagocytosis. Inflammatory stimuli, including multiple cytokines Toll-like receptor (TLR) ligands, augment efficiency fully...
CD2 is largely described to promote T cell activation when engaged by its ligands, CD48 in mice and CD58 humans, that are present on antigen-presenting cells (APCs). However, both also expressed cells. By generating new knockout mouse strains lacking or the C57BL/6 background, we determined whereas was necessary for activation, ligand not required APCs. Rather, needed One exception during cytotoxicity, which Fluorescence resonance energy transfer (FRET) studies nonimmune provided evidence...
Background: 5-aza-2'-deoxycytidine (5Aza), a DNA methyltransferase (DNMT) inhibitor, could activate tumor adaptive immunity to inhibit progression. However, the molecular mechanisms by which 5Aza regulates immune microenvironment are still not fully understood. Methods: The role of in peritoneal carcinomatosis (PC) colorectal cancer (CRC) was investigated. effects on macrophage activation were studied flow cytometry, real-time PCR, Western blotting assays, and Drug Affinity Responsive Target...
Ciliogenesis is a fundamental biological process central to human health. Precisely how this coordinated with the cell cycle remains an open question. We report that nephrocystin-5 (NPHP5/IQCB1), positive regulator of ciliogenesis, stable and low turnover protein subjected cycles ubiquitination deubiquitination. NPHP5 directly binds deubiquitinating enzyme USP9X/FAM two E3 ubiquitin ligases BBS11/TRIM32 MARCH7/axotrophin. undergoes K63 in dependent manner K48/K63 upon USP9X depletion or...
Infection of mice by mouse cytomegalovirus (MCMV) triggers activation and expansion Ly49H+ natural killer (NK) cells, which are virus specific considered to be "adaptive" or "memory" NK cells. Here, we find that signaling lymphocytic molecule family receptors (SFRs), a group hematopoietic cell-restricted receptors, essential for the cells after MCMV infection. This activity is largely mediated CD48, an SFR broadly expressed on displaying augmented expression It also dependent CD48...
Hepatic stellate cells (HSCs) play a key role in liver fibrosis. CCAAT/enhancer-binding proteins-alpha (C/EBP-alpha) can inhibit HSCs activation vitro, as described our previous study. However, little is known about the vivo effect of C/EBP-alpha gene hepatic fibrosis.Male BALB/c mice were injected by hydrodynamic protocol with pIRES2-EGFP-C/EBPalpha expression vector from first to fourth week (early intervention) or ninth 12th (late after CCl(4) injection, respectively. Successful...
Expression of the signaling lymphocytic activation molecule (SLAM)–associated protein (SAP) is critical for germinal center (GC) reaction and T cell–dependent antibody production. However, when SAP expressed normally, role associated SLAM family receptors (SFRs) in these processes nebulous. Herein, we established that presence SAP, SFRs suppressed expansion GC but facilitated generation antigen-specific B cells antibodies. favored antigen-reactive antibodies by boosting expression...
Summary Adrenomedullin (AM), a peptide isolated from human pheochromocytoma, can be produced and secreted by various types of cells including hepatic stellate (HSCs), its possible role in HSCs is not clear now. In the present study, interactive regulation between transforming growth factor (TGF)‐β1 AM effect on TGF‐β1‐induced matrix metalloproteinase (MMP)‐2 expression were investigated. TGF‐β1 inhibited gene transcript level mutually (real‐time reverse transcription‐polymerase chain...
Thymocyte-expressed, positive selection-associated 1 (Tespa1) plays an important role in both T cell receptor (TCR)-driven thymocyte development and the FcεRI-mediated activation of mast cells. Herein, we show that lack Tespa1 does not impair B but dampens vitro proliferation cells induced by cell-dependent (TD) antigens, significantly reduces serum antibody concentrations vivo impairs germinal center formation aged TD antigen-immunized mice. We also provide evidence dysregulated signaling...
Aim: To investigate whether fenretinide, a clinically proved apoptosis‐inducing chemopreventive agent in tumor cells, can induce apoptosis hepatic stellate cells (HSCs) and resolve fibrosis. Methods: CCl 4 ‐induced liver fibrosis mice rat activated (HSC‐T6) as well hepatocytes (BRL‐3A) were studied. Results: The duplex staining of proliferating cell nuclear antigen α‐ smooth muscle actin or terminal deoxynucleotidyl transferase‐mediated deoxyuridine triphosphate nick‐end labeling...
Abstract Introduction: Improved understanding of the interplay between immune system and cancer would lead to more adequate response prediction immune-checkpoint blockade (ICB) treatment. Furthermore, insight into interactions allow identifying biomarkers for ICB. Single-cell RNA sequencing has emerged as a powerful technology characterize heterogeneity in large population cells opens up opportunities predict therapy. Purpose: Tracking effect single dose Pembrolizumab on tumor...