A5107117868- Infectious Diseases and Tuberculosis
- Tuberculosis Research and Epidemiology
- Ocular Diseases and Behçet’s Syndrome
- Mycobacterium research and diagnosis
- Diagnosis and treatment of tuberculosis
- Orthopedic Infections and Treatments
- Retinal and Optic Conditions
- Ocular Infections and Treatments
- Systemic Lupus Erythematosus Research
- Cytomegalovirus and herpesvirus research
- Bacterial Infections and Vaccines
- Sarcoidosis and Beryllium Toxicity Research
- Vasculitis and related conditions
- Pneumocystis jirovecii pneumonia detection and treatment
- Biosensors and Analytical Detection
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Histiocytic Disorders and Treatments
- Hematological disorders and diagnostics
- Retinal Diseases and Treatments
- Infectious Diseases and Mycology
- Medical Imaging and Pathology Studies
- Advanced Sensor and Energy Harvesting Materials
- Agricultural Economics and Practices
- Seed and Plant Biochemistry
- Immune Cell Function and Interaction
Post Graduate Institute of Medical Education and Research
2015-2024
Collaborative Group (United States)
2024
Dr. Ram Manohar Lohia Avadh University
2023
Dr. Sarvepalli Radhakrishnan Rajasthan Ayurved University
2023
Banasthali University
2023
Jeju National University
2022-2023
Swami Keshwanand Rajasthan Agricultural University
2023
Maharana Pratap University of Agriculture and Technology
2023
Indian Institute of Engineering Science and Technology, Shibpur
2023
Indira Gandhi Medical College
2014
Abstract Background The objective of this study was to report the use multi-targeted polymerase chain reaction (PCR) in diagnosis presumed tubercular uveitis. Multi-targeted PCR using three targets specific for Mycobacterium tuberculosis , i.e., IS6110 MPB64 and protein b performed on intraocular fluid samples 25 subjects. Nine had uveitis, six inflammation secondary a nontubercular etiology (disease controls), ten no evidence (normal controls). As described previously, response...
Purpose: To analyze the role of polymerase chain reaction (PCR) ocular fluids in management tubercular (TB) anterior, intermediate, posterior, and panuveitis.Methods: In Collaborative Ocular Tuberculosis Study (COTS)-1 (25 centers, n = 962), patients with TB-related uveitis were included. 59 undergoing PCR intraocular (18 females; 53 Asian Indians) included.Results: (6.13%) COTS-1 underwent analysis. was positive for Mycobacterium TB 33 (23 males; all Indians). 26 negative males). Eight had...
Purpose: To report epidemiology of uveitis in a tertiary-care referral institute North India.Methods: Hospital records patients with between June 2011 and September 2014 were retrospectively analyzed.Results: Of the 1912 patients, 56.64% males. Anterior (43.04%) was most common manifestation followed by posterior (24.58%), panuveitis (16.21%), intermediate (10.66%). Specific etiologies could be ascertained 60.56%. Tuberculosis (22.9%) infectious HLA-B27-associated (9.46%) among...
Methods: Eighty-eight patients were prospectively enrolled into 3 groups: 28 with so-called Eales’ disease (group A); 30 positive controls specific uveitis syndromes B), and negative C). The undiluted vitreous humor samples collected subjected to real-time PCR assay for MPB64 gene of Mycobacterium tuberculosis (MTB) load quantified.
Purpose: To contribute a global description of the spectrum choroidal involvement in tubercular uveitis (TBU).Methods: Retrospective cohort study TBU patients with from 25 centers between January 2004 and December 2014. Medical records minimum follow-up 1 year were reviewed.Results: 245 included. The phenotypic variations included serpiginous-like choroiditis (SLC) (46%), tuberculoma (13.5%), multifocal (MFC) (9.4%), ampiginous (9%), among others. 219 treated anti-tubercular therapy (ATT) (n...
Mycobacterium tuberculosis, the causative agent of accounts for 1.5 million human deaths annually worldwide. Despite efforts to eradicate it still remains a deadly disease. The two best characterized strains M. virulent H37Rv and avirulent H37Ra, provide unique platform investigate biochemical signaling pathways associated with pathogenicity. To delineate biomolecular dynamics that may account pathogenicity attenuation virulence in we compared proteome phosphoproteome profiles H37Ra strains....
To assess choroidal vascular changes among patients with tubercular multifocal serpiginoid choroiditis (TB MSC) using previously validated techniques.Patients TB MSC (n = 18) and healthy controls 30) underwent enhanced-depth imaging optical coherence tomography (EDI-OCT) imaging. Using algorithm of image binarization, EDI-OCT scans were segmented to derive total area, luminal stromal vascularity index (CVI).There was a statistically significant difference in the CVI between (66.90 ± 1.77%)...
Delay in diagnosing osteoarticular tuberculosis (OATB) contributes significantly to morbidity by causing disfiguration and neurological sequelae. The delay caused conventional culture the expertise expense involved other nucleic acid based tests, make LAMP (loop-mediated isothermal amplification) assay a favorable middle path. We evaluated using IS6110 MPB64 for rapid diagnosis of OATB comparing with PCR culture. was performed on 140 synovial fluid pus samples (10 culture-positive proven...
To study the safety and efficacy of intravitreal injection dexamethasone implant in management posterior segment involvement tubercular uveitis (TBU).In this study, retrospective analysis for various indications such as cystoid macular edema (CME), vitritis, or paradoxical worsening TB-related intermediate uveitis, retinal vasculitis, multifocal serpiginoid choroiditis (MSC) was performed.The included 17 patients (19 eyes; 7 males). IOP increased from 14.88 ± 2.68 mm Hg to 16.4 5.82 (p =...
Background: Tuberculosis (TB) is a major infection that can affect the eye as first and sole presentation without features of systemic disease. Controversy exists regarding diagnosis management tubercular uveitis (TBU), further compounded by regional variations in disease expression. Purpose: Collaborative Ocular Study (COTS)-1 aims to address knowledge deficits through collaboration amongst specialists across globe sharing data patients with TBU presented at participating centers from...
<ns3:p>The pathogenesis of Tuberculous meningitis (TBM) is poorly understood, but contemporary molecular biology technologies have allowed for recent improvements in our understanding TBM. For instance, neutrophils appear to play a significant role the immunopathogenesis TBM, and either paucity or an excess inflammation can be detrimental Further, severity HIV-associated immunosuppression important determinant inflammatory response; patients with advanced (CD4+ T-cell count <150 cells/μL)...
Background Truenat MTB Plus assay was evaluated for diagnosing ocular tuberculosis (OTB) and detecting multi-drug resistant (MDR) extremely-drug (XDR) OTB.Methods A total of 75 vitreous fluid specimens [five confirmed OTB, 40 clinically suspected OTB 30 controls] were subjected to Plus, multiplex PCR, Xpert Ultra. Chips used rifampicin, isoniazid, fluoroquinolone bedaquiline resistance. The performance compared against culture, composite reference standard, gene sequencing.Results overall...
Background: Rapid and specific diagnosis of gastrointestinal tuberculosis (GITB) is utmost importance. Aim: To evaluate Multiplex PCR (MPCR) using MPB64 IS6110 primers for M. rapid GITB. Materials Methods: MPCR was performed on colonoscopy biopsy specimens 11 GITB confirmed (culture/AFB/histopathology positive), 29 suspected 30 Non (control group) patients. Results: had sensitivity specificity 90% 100% cases. In clinically diagnosed but unconfirmed cases, positive in 72.41%. negative all...
Data on the use of Xpert Mtb/Rif for diagnosis intestinal tuberculosis is sparse. We report utility testing (ITB) in patients with ileocecal ulcers.We performed a retrospective analysis ulcers and suspected to have ITB whom tissue was performed. The were divided into two groups: those final other diagnosis. These compared clinical features presentation. sensitivity, specificity, positive predictive value, negative value calculated.Of 40 studied, 23 women mean age 32.92 ± 12.78 years....
Background: Rapid and specific diagnosis of tubercular meningitis (TBM) is utmost importance. Aim: To evaluate polymerase chain reaction (PCR) using protein b primers directed against M. tuberculosis for the tuberculous meningitis. Materials Methods: PCR was performed in ten patients with confirmed TBM (culture positive), 60 clinically suspected 40 no (control group). Results: Protein had a sensitivity 90% specificity 100% TBM. In diagnosed patients, positive 49 (81.7%) patients. The overall...
The identification of anaerobes, Mycobacterium and Nocardia species, moulds by MALDI-TOF-MS remains a challenge. This study aimed to evaluate the performance MALDI-TOF in these organisms.