A5002472975- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Metabolism, Diabetes, and Cancer
- Cardiovascular Function and Risk Factors
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- RNA regulation and disease
- Angiogenesis and VEGF in Cancer
- Coronary Interventions and Diagnostics
- Protein Kinase Regulation and GTPase Signaling
- MicroRNA in disease regulation
- Peripheral Artery Disease Management
- Phytochemical Studies and Bioactivities
- Sirtuins and Resveratrol in Medicine
- NF-κB Signaling Pathways
- Connexins and lens biology
- Heat shock proteins research
- Muscle Physiology and Disorders
- Long-Term Effects of COVID-19
- Cancer-related Molecular Pathways
- Connective tissue disorders research
- Whipple's Disease and Interleukins
- Sulfur Compounds in Biology
- 14-3-3 protein interactions
Nanjing Medical University
2020-2024
Georgia State University
2016-2024
China Pharmaceutical University
2024
Huazhong University of Science and Technology
2008-2018
Medical College of Wisconsin
2017
Wuhan Union Hospital
2017
University of Oklahoma Health Sciences Center
2015-2016
Zhejiang University
2015-2016
Second Affiliated Hospital of Zhejiang University
2016
Qilu Hospital of Shandong University
2016
Background: FUN14 domain containing 1 (FUNDC1) is a highly conserved outer mitochondrial membrane protein. The aim of this study to examine whether FUNDC1 modulates the mitochondria-associated endoplasmic reticulum (ER) membranes (MAMs), morphology, and function in cardiomyocytes intact hearts. Methods: impacts on MAMs formation cardiac functions were studied mouse neonatal cardiomyocytes, mice with cardiomyocyte-specific Fundc1 gene knockout ( f/Y /Cre αMyHC+/− ), tissues patients heart...
Background: Fundc1 (FUN14 domain containing 1), an outer mitochondrial membrane protein, is important for mitophagy and mitochondria-associated endoplasmic reticulum membranes (MAMs). The roles of MAMs in diabetic hearts remain unknown. aims this study, therefore, were to determine whether the diabetes mellitus–induced expression could increase MAM formation, disruption formation improves cardiac function. Methods: Levels FUNDC1 examined from patients nondiabetic donors. Fundc1-induced heart...
Endoplasmic reticulum stress is an evolutionarily conserved cell response associated with numerous diseases, including cardiac hypertrophy and heart failure. The major endoplasmic signaling pathway causing involves sensor PERK (protein kinase-like kinase) eIF2α-ATF4-CHOP signaling. Here, we describe a non-canonical, AGGF1-mediated regulatory system for increased p-eIF2α ATF4 decreased sXBP1 CHOP. Specifically, see reduced AGGF1 level consistently induction of in mouse models human patients...
Abstract Although angiotensin II (AngII) is known to cause renal injury and fibrosis, the underlying mechanisms remain poorly characterized. Here we show that hypertensive nephropathy (HN) patients AngII-infused mice exhibit elevated levels of circulating miR103a-3p. We observe a positive correlation between miR-103a-3p AngII-induced dysfunction. suppresses expression sucrose non-fermentable-related serine/threonine-protein kinase SNRK in glomerular endothelial cells, glomeruli HN reduced...
Atherosclerotic calcification is highly linked with plaque rapture. How regulated poorly characterized.We sought to determine the contributions of AMP-activated protein kinase (AMPK) in atherosclerotic calcification.Aortic was evaluated aortic roots and brachiocephalic arteries prone ApoE(-/-) mice or dual deficiencies ApoE AMPKα isoforms whole body (ApoE(-/-)/AMPKα1(-/-) ApoE(-/-)/AMPKα2(-/-)) vascular smooth muscle cell (VSMC)-specific macrophage-specific knockout AMPKα1 fed Western diet...
AGGF1 is an angiogenic factor with therapeutic potential to treat coronary artery disease (CAD) and myocardial infarction (MI). However, the underlying mechanism for AGGF1-mediated angiogenesis unknown. Here, we show first time that activates autophagy, a housekeeping catabolic cellular process, in endothelial cells (ECs), HL1, H9C2, vascular smooth muscle cells. Studies Atg5 small interfering RNA (siRNA) autophagy inhibitors bafilomycin A1 (Baf) chloroquine demonstrate required EC...
AMP-activated protein kinase (AMPK) has been reported to play a protective role in atherosclerosis. However, whether AMPKα2 controls atherosclerotic plaque stability remains unknown.The aim of this study was evaluate the impact deletion on advanced atherosclerosis at brachiocephalic arteries and elucidate underlying mechanisms.Features markers for contractile or synthetic vascular smooth muscle cell (VSMC) phenotypes were monitored from Apoe(-/-)AMPKα2(-/-) mice VSMC-specific AMPKα2(-/-) an...
Melatonin (N-acetyl-5-methoxytryptamine) has been shown to have a cardioprotective effect against myocarditis. However, the mechanisms underlying protective role of melatonin (MLT) in sepsis-induced myocarditis are yet be revealed. In this study, MLT was administrated mice, 14 days before cecal ligation puncture surgery. Echocardiography results showed that alleviated cardiac dysfunction Furthermore, reduced inflammation by inhibiting expression Il-1α, Il-1β, Il-6, and Mcp-1 messenger RNA...
Atrial fibrillation (AF) is the most common cardiac arrhythmia at clinic. Recent GWAS identified several variants associated with AF, but they account for <10% of heritability. Gene-gene interaction assumed to a significant portion missing Among loci only three were replicated in Chinese Han population, including SNP rs2106261 (G/A substitution) ZFHX3, rs2200733 (C/T near PITX2c, and rs3807989 (A/G CAV1. Thus, we analyzed among these AF loci. We demonstrated between independent populations...
Background Peripheral arterial disease (PAD) is a common accounting for about 12% of the adult population, and causes significant morbidity mortality. Therapeutic angiogenesis using angiogenic factors has been considered to be potential treatment option PAD patients. In this study, we assessed new factor AGGF1 therapeutic in critical limb ischemia model mice PAD. Methods Results We generated unilateral hindlimb by ligation right iliac artery femoral artery. Ischemic with intrasmuscular...
Abstract Dioscin has been widely used in clinics for coronary artery disease (CAD) treatment years China. However, the underlying mechanism Dioscin‐mediated cardioprotective effect not elucidated. Here, we showed that significantly rescues cardiac function mouse model of myocardial infarction (MI), accompanied by reduction fibrosis and apoptosis, resulting from elevated angiogenesis. Mechanistically, promotes proliferation migration hypoxic endothelial cells via up‐regulation lncRNA MANTIS ,...
Background— This study tested the hypothesis whether endoplasmic reticulum (ER) stress/C/EBP homologous protein (CHOP) signaling is linked with coxsackievirus B3 (CVB3)–induced acute viral myocarditis (AVMC) in vivo. Methods and Results— AVMC was induced by intraperitoneal injection of 1000 tissue culture infectious dose (TCID 50 ) CVB3 virus mice. In mouse hearts (n=11), ER stress CHOP were significantly activated, to induction proapoptotic including reduction Bcl-2, activation Bax caspase...
Objective— SNRK (sucrose nonfermenting 1-related kinase) is a novel member of the AMPK (adenosine monophosphate-activated protein kinase)-related superfamily that activated in process angiogenesis. Currently, little known about function angiogenesis physiological and pathological conditions. Approach Results— In this study, Snrk global heterozygous knockout mice, retina neovessel formation after hindlimb ischemia were suppressed. Consistently, mice with endothelial cell (EC)-specific...
Macroautophagy/autophagy, a fundamental process for degradation of macromolecules and organelles, occurs constitutively at basal level is upregulated in response to stress. Whether autophagy regulates protein acetylation microtubule stability vascular smooth muscle cells (VSMCs) migration, however, remains unknown. Here, we demonstrate that the histone acetyltransferase KAT2A/GCN5 (lysine 2) binds directly autophagosome MAP1LC3/LC3 (microtubule associated 1 light chain 3) via conserved...
The messenger RNA (mRNA) 3′ untranslated regions (3′ UTRs), as cis-regulated elements bound by microRNAs (miRNAs), affect their gene translation. However, the role of trans-regulation UTRs during heart dysfunction remains elusive. Compared with administration angiogenic factor G-patch and forkhead-associate domains 1 (Aggf1), ectopic expression Aggf1 its UTR significantly suppressed cardiac in angiotensin II-infused mice, upregulated both myeloid cell leukemia (Mcl1). Along UTRs, Mcl1 mRNAs...
Diabetes mellitus (DM) has been demonstrated to have a strong association with heart failure. Conventional echocardiographic analysis cannot sensitively monitor cardiac dysfunction in type I diabetic Akita hearts, but the phenotype of failure is observed molecular levels during early stages.Male (Ins2WT/C96Y) mice were monitored imaging at various ages, and then conventional speckle-tracking based strain analyses.With analyses, showed changes average global radial age 12 weeks, as well...
Myocardial infarction is one of the most severe heart diseases, leading to sudden death. Currently, angiography and stenting are widely performed in clinics, yet more effective treatment still needed. Herein, we presented that dioscin, a natural product, showed protective effect on infarcted hearts via mitochondrial maintenance. Upon dioscin treatment, cardiac dysfunction was alleviated, remodeling prevented. Mechanistically, disocin maintains mitochondria function through maintenance Kreb's...
<b><i>Background/Aims:</i></b> The renal resistive index (RI) is a novel candidate as injury prognostic indicator, but it remains unclear how RI levels correspond to in diabetic nephropathy. <b><i>Methods:</i></b> To examine this issue, we compared 8-week-old male C57BL/6 mice fed with high-fat diet (HFD) versus chow (CHD) for 16 weeks. At 8 and 12 weeks, the glomerular filtration rate (GFR), urinary albumin-to-creatinine ratio (UACR),...
Background The SNRK (sucrose-nonfermenting-related kinase) enzyme is critical for cardiac function. However, the underlying cause heart failure observed in
Abstract Interleukin-27 (IL-27) is an important cytokine in inflammatory diseases, including coronary artery disease (CAD). To explore the precise role of IL-27 CAD, we investigated genetic association between IL27 and CAD GeneID Chinese Han population. A two-stage case control analysis was performed for 3075 cases 2802 controls. Logistic regression used to adjust traditional risk factors CAD. Results showed that a promoter variant, rs153109, tended be marginally associated with discovery...