A5011044774- Cancer Genomics and Diagnostics
- Lymphoma Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Chronic Lymphocytic Leukemia Research
- RNA modifications and cancer
- HER2/EGFR in Cancer Research
- Viral-associated cancers and disorders
- Reproductive tract infections research
- Cancer-related Molecular Pathways
- Pancreatic and Hepatic Oncology Research
- Cervical Cancer and HPV Research
- Neuroendocrine Tumor Research Advances
- Immunodeficiency and Autoimmune Disorders
- Preterm Birth and Chorioamnionitis
- Monoclonal and Polyclonal Antibodies Research
- Prenatal Screening and Diagnostics
- Neonatal Respiratory Health Research
- Bladder and Urothelial Cancer Treatments
- Epigenetics and DNA Methylation
- Renal cell carcinoma treatment
- Tumors and Oncological Cases
- Gestational Trophoblastic Disease Studies
- Neuroblastoma Research and Treatments
- Glycosylation and Glycoproteins Research
- Gastric Cancer Management and Outcomes
Vita-Salute San Raffaele University
2015-2025
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2015-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2015-2025
IRCCS Ospedale San Raffaele
2018-2023
Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia
2021
San Raffaele University of Rome
2008-2020
University of Siena
1997-2009
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"
2007
Centro di Riferimento Oncologico
2007
Ospedale Policlinico San Martino
2003
Chromosomal translocations that encode fusion oncoproteins have been observed consistently in leukemias/lymphomas and sarcomas but not carcinomas, the most common human cancers. Here, we report t(2;3)(q13;p25), a translocation identified subset of thyroid follicular results DNA binding domains transcription factor PAX8 to A F peroxisome proliferator–activated receptor (PPAR) γ1. PAX8-PPARγ1 mRNA protein were detected 5 8 carcinomas 20 adenomas, 10 papillary or multinodular hyperplasias....
Myoepithelial cells (MCs) constitute the basal cell layer of normal mammary epithelia, and their identification is particular diagnostic value because they are retained in most benign lesions while being lost malignancy. Several MC immunocytochemical markers currently available for purposes, with special reference to smooth muscle-related antigens. p63 a member p53 gene family, its germline mutations associated severe developmental defects both rodents humans. Different isoforms have been...
Abstract BACKGROUND Syndecan‐1 is a transmembrane heparan sulphate proteoglycan that involved in cell–cell adhesion, organization of cell–matrix and regulation growth factor signaling. METHODS Specimens from 254 consecutive breast carcinoma (BC) cases (110 N0, 144 N1/2) with long‐term follow‐up (median, 95 months) were immunostained for syndecan‐1, estrogen receptor (ER), progesterone (PgR), p53; 154 cases, c‐erbB‐2 status was known. mRNA protein expression also evaluated 20 tissue samples...
To detect the presence of MYD88 L265P mutation in aqueous humor patients with cytologically proven vitreoretinal lymphoma.Eight consecutive bilateral lymphoma (16 eyes) were prospectively evaluated. Genomic DNA was extracted from samples after paracentesis and vitreous diagnostic vitrectomy. codon 265 investigated by both amplification-refractory system polymerase chain reaction approach pyrosequencing assay all 6 patients. A control group 8 age-matched established diagnosis noninfectious...
Chlamydia psittaci (Cp) has been associated to ocular adnexal lymphomas (OAL) with variable geographic distribution. Herein, we used multiple detection tools identify Cp elementary bodies-containing cell and assess prevalence in both nodal extranodal lymphomas.TETR-PCR, immunohistochemistry, immunofluorescence, electron microscopy, laser-capture microdissection were done 35 OALs define their effect and, moreover, the cellular carrier. was screened by TETR-PCR 205 extraorbital 135...
Multiple myeloma develops primarily inside the bone marrow microenvironment, that confers pro-survival signals and drug resistance. 3D cultures reproduce multiple myeloma-bone interactions are needed to fully investigate pathogenesis response drugs. To this purpose, we exploited Rotary Cell Culture System bioreactor technology for co-cultures in gelatin scaffolds. The model was validated with cell lines that, as assessed by histochemical electron-microscopic analyses, engaged contacts...
ABSTRACT Spitz lesions display a set of genetic alterations that differ from classical melanocytic lesions: examples include mutations in HRAS and fusions involving ALK , ROS1 MET MAP3K8 BRAF the NTRK genes. We present spindle cell/Reed nevus with atypical junctional features an NTRK2 translocation patient unusual age. The was 61‐year‐old man pigmented brown flat 6 mm lesion growing on skin over left scapula. composed spindled epithelioid melanocytes arranged nests some scattered focal...
Ocular adnexal MALT lymphoma (OAML) is linked to Chlamydophila psittaci (Cp) infection. Viability and infectivity of Cp, demonstrated by growth in culture, has not been yet investigated these patients. We conducted a single-center prospective case-control study assess the prevalence, viability Cp 20 OAML patients 42 blood donors registered 6-month period. The presence conjunctival swabs peripheral mononuclear cells (PBMC) was assessed TETR-PCR vitro cultures. From an epidemiological point...
The ability to identify the broadest range of targetable gene fusions is crucial facilitate personalized therapy selection for advanced lung adenocarcinoma (LuADs) patients harboring receptor tyrosine kinase (RTK) genomic alterations. In order evaluate most effective testing approach LuAD fusion detection, we analyzed 210 NSCLC selected clinical samples, comparing in situ (Fluorescence Situ Hybridization, FISH, and ImmunoHistoChemistry, IHC) molecular (targeted RNA Next-Generation...
Abstract Malignant Mesothelioma is a highly aggressive cancer, which difficult to diagnose and treat. Here we describe the molecular, cellular morphological characterization of syngeneic system consisting murine AB1, AB12 AB22 mesothelioma cells injected in immunocompetent BALB/c mice, allows study interplay tumor with immune system. Murine cells, like human ones, respond exogenous High Mobility Group Box 1 protein, Damage-Associated Molecular Pattern that acts as chemoattractant for...
Assessment of HRD status is now essential for ovarian cancer patient management. A relevant percentage high-grade serous carcinoma (HGSC) characterized by HRD, which caused genetic alterations in the homologous recombination repair (HRR) pathway. Recent trials have shown that not only patients with pathogenic/likely pathogenic BRCA variants, but also BRCAwt/HRD patients, are sensitive to PARPis and platinum therapy. The most common test Myriad MyChoice CDx, there a pressing need offer an...
Renal-cell carcinoma (RCC) constitutes a heterogeneous group of tumors with specific chromosome aberrations. Recently, new small RCC, occurring in children and young adults, has been described as characterized by t(6;11)(p21;q12). It shown that this translocation results the fusion 5' portion ALPHA gene (11q12) transcription factor TFEB (6p21). Herewith, we report first complete cytogenetic molecular characterization t(6;11)-positive RCC an adult patient, 54-year-old woman. The tumor was...