A5061884600- Reproductive Biology and Fertility
- Ovarian function and disorders
- Sperm and Testicular Function
- Coronary Interventions and Diagnostics
- Renal and related cancers
- Prenatal Screening and Diagnostics
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive System and Pregnancy
- Peripheral Artery Disease Management
- Cardiac Imaging and Diagnostics
- Genetic Syndromes and Imprinting
- Acute Myocardial Infarction Research
- Molecular Biology Techniques and Applications
- Viral Infections and Vectors
- Gynecological conditions and treatments
- Streptococcal Infections and Treatments
- Tissue Engineering and Regenerative Medicine
- Reproductive Health and Technologies
- Hematological disorders and diagnostics
- Cerebrovascular and Carotid Artery Diseases
- Sexual function and dysfunction studies
- Atherosclerosis and Cardiovascular Diseases
- Urinary Bladder and Prostate Research
Ljubljana University Medical Centre
2025
Vrije Universiteit Brussel
2009-2023
KU Leuven
2019
Universitair Ziekenhuis Brussel
2006-2012
Massachusetts General Hospital
2012
Codarts Rotterdam
2012
University of the Basque Country
2011
Ayuntamiento de Bilbao
2011
Assaf Harofeh Medical Center
2011
OLVG
2011
The purpose of this document is to make the output International Working Group for Intravascular Optical Coherence Tomography (IWG-IVOCT) Standardization and Validation available medical scientific communities, through a peer-reviewed publication, in interest improving diagnosis treatment patients with atherosclerosis, including coronary artery disease. optical coherence tomography (IVOCT) catheter-based modality that acquires images at resolution ∼10 μm, enabling visualization blood vessel...
Epidemiological changes and the ongoing expansion of diagnostic armamentarium warrant a regular update spectrum diseases that present as prolonged febrile illnesses.We prospectively collected series 290 immunocompetent patients referred to our university hospital between 1990 1999 with illness (temperature >38.3 degrees C) uncertain cause, lasting at least 3 weeks. Patients were categorized in 4 groups according timing diagnosis: early diagnosis (within in-hospital days or outpatient...
Gene expression of cumulus cells (CC) could predict oocyte developmental quality. Knowledge the genes involved in determining quality is scanty. The aim was to correlate clinical and biological characteristics during ovarian stimulation with 10 selected CC. Sixty-three ICSI patients were stimulated GnRH-agonist plus highly purified hMG (n = 35) or recombinant FSH 28). Thirteen variables analyzed: Age, BMI, duration stimulation, serum concentrations progesterone, 17β-estradiol, LH on day hCG,...
BACKGROUNDCumulus cell (CC) gene expression is suggested as a non-invasive analysis method to predict oocyte competence. There are, however, important between-patient differences in CC expression. These can be compensated when results are combined with patient and cycle characteristics using multiple regression model.
This study addresses the role of cAMP hydrolytic isoenzyme phosphodiesterase type 3 (PDE 3) modulation on human oocyte maturation in vitro. Presence A 3A) mRNA was confirmed germinal vesicle-stage (GV) oocytes. Making use a selective PDE inhibitor, Org 9935 (10 μM), oocytes retrieved from immature follicles were arrested prophase I with high efficiency for up to 72 h. Cumulus complexes (COCs) follicular phase cycle before or after exposure endogenous LH hCG administration vivo and randomly...
High-risk acute pulmonary embolism (PE) is a life-threatening condition necessitating hemodynamic stabilization and rapid restoration of perfusion. In this context, evidence regarding the benefit advanced circulatory support recanalization strategies still limited. observational study, we assessed data 1060 patients treated for high-risk PE with 991 being included in target trial emulation to investigate all-cause in-hospital mortality estimates different treatment strategies. The four...
A reproducible two-step culture system for isolated mouse ovarian follicles smaller than 100 microm (type 3a follicles) was designed. First, were grown in single droplets of alpha-minimal essential medium (MEM) without (deoxy)ribonucleosides at a lower concentration fetal bovine serum (FBS; 1%) 6 days with mechanical prohibition thecal cell attachment. Growing reaching least transferred to alpha-MEM enriched higher (5%) FBS allow attachment and cultured subsequently an additional 12 days....
Imprinted genes are differentially methylated during gametogenesis to allow parental-specific monoallelic expression of genes. During mouse oogenesis, DNA methylation at imprinted is established the transition from primordial antral follicle stages. Studies in human and suggest aberrant imprinting oocytes following vitro maturation after superovulation with high doses gonadotrophines. The exact mechanisms leading unknown. We examined status regions key (by bisulphite sequencing) metaphase II...
Imprinted genes are differentially methylated during gametogenesis to allow parent-of-origin-specific monoallelic expression. We previously demonstrated establishment of normal imprinting at four key imprinted in mouse metaphase II oocytes after vitro follicle culture. Commercially available culture media feature a wide range methyl donor levels. The aim the present study was examine effect low (methionine, vitamin B(12), folic acid, choline, and B(6)) levels on acquisition DNA methylation...
Oocyte cytoplasmic maturation is influenced by the quantity of synthesized RNA and proteins accumulated stored during growth. Transcriptional repression degradation transcripts occur oocyte nuclear maturation, prolonged transcriptional arrest might compromise stores for early development. quantification key genes in oocytes be valuable when setting up vitro cultures that lack normal hormonal interplay found vivo. This study quantifies gene expression levels relation to follicle culture time...
Follicle-stimulating hormone (FSH) and oocyte-secreted factors influence granulosa cell differentiation follicle development. Whereas FSH stimulates the expression of mural transcripts, regulate specific cumulus genes suppress appearance transcripts. This study addresses extent to which clinically relevant changes in doses applied during antral development vitro could alter oocyte A 12-day culture system mouse ovarian preantral follicles can grow preovulatory was used. The following three...
Within the context of an oocyte in vitro maturation (IVM) program for reproductive treatment, cumulus complexes (COCs) derived from follicles <6 mm patients with PCOS were matured vitro. Key transcripts related to meiotic (FSHR, LHCGR, EGFR, PGR) and competence (AREG, ADAMTS, HAS2, PTGS2) quantified cells (CCs) before after maturation. Control CC samples collected normo-ovulatory who had undergone conventional gonadotrophin stimulation IVF/ICSI. Additional control a non-stimulated condition...
What are the changes in human embryos, terms of morphology and gene expression, upon attachment to endometrial epithelial cells? Apposition adhesion blastocysts cells predominantly initiated at embryonic pole these steps associated with expression extracellular matrix (ECM) genes embryo. Both murine embryos have been co-cultured Ishikawa cells, although has not yet investigated an vitro implantation model. Vitrified were warmed for up 48 h a model cell line receptive epithelium. Six days...
Abstract The present study was carried out to de novo glutathione (GSH) synthesis and evaluate the effect of stimulating GSH during in vitro maturation (IVM) adult prepubertal mouse oocytes on embryo developmental rate. Adult (8 weeks old) mice (24–26 days were primed with 5 IU PMSG retrieved from ovary 48 hr later for IVM. After IVM (18 hr) Cumulus oocyte complexes (COC) fertilized (IVF) culture (IVC) order observe development. medium supplemented with: 0, 25, 50, 100, or 200 μM cysteamine....
Meiotic maturation of the oocyte is a timed sequence events induced by ovulatory LH surge. In vitro oocytes known to alter meiotic time course. This study documented timing meiosis in grown for 12 days, from preantral follicle stage onward, and influence an oil overlay. oil-free culture, stability metaphase II spindle was further explored determine postovulatory aging events. After stimulus, vitro-grown were collected at 2-h intervals spanning period (0-18 h) 3-h during early (18-27 h)....
Cumulus cell (CC) gene expression is being explored as an additional method to morphological scoring choose the embryo with highest chance pregnancy. In 47 ICSI patients single transfer (SET), from which individual CC samples had been stored, 12 genes using QPCR were retrospectively analyzed. The at same occasion also used validate a previously obtained pregnancy prediction model comprising three (ephrin-B2 (EFNB2), calcium/calmodulin-dependent protein kinase ID, stanniocalcin 1). Latter...
Do the mRNA expression levels of zona pellucida (ZP) genes, ZP1, 2, 3 and 4 in oocyte cumulus cells (CC) reveal relevant information on oocyte?The ZP human oocytes is related to maturity, inner layer (IL) retardance fertilization capacity.ZP structure birefringence provide useful cytoplasmic maturation, developmental competence for embryonic growth, blastocyst formation pregnancy. In order understand molecular basis morphological changes ZP, current study, polarized light microscopy (PLM)...
Growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15), and anti-Müllerian hormone (AMH) play an important role in the primary to secondary follicle transition activation vivo. In organ culture of neonatal mouse ovaries, it was observed that significantly fewer follicles develop stage. The objectives this study were: (1) compare ovarian follicular populations between organ-cultured ovaries freshly isolated age-matched control ovaries; (2) quantify RNA levels Gdf9,...
Imprinted genes are differentially methylated during gametogenesis to allow parent-of-origin-specific monoallelic expression. Follicle culture under oil overlay has been associated with altered imprinting establishment in mouse oocytes. We previously demonstrated normal at four key imprinted oocytes grown and matured a long-term vitro follicle system without overlay. Ammonium (300 microM) linked aberrant preimplantation embryo culture. Compared oil, mineral led dramatic increase ammonia...
Extracellular matrix (ECM) formation by cumulus cells is an important process that determines fertilization and embryo quality. Several collagen types are present in the ovarian follicular ECM related to proliferation, steroidogenesis, luteinization. In vitro mouse follicles can optimally grow provide developmentally competent oocytes with 10 IU/L recombinant follicle-stimulating hormone (rFSH). As a model for superovulation, experiments 100 rFSH or highly purified menotropin (HP-hMG)...
Abstract STUDY QUESTION Is there an increase in the total number of metaphase II (MII) oocytes between a conventional ovarian stimulation (OS) and double uninterrupted stimulation? SUMMARY ANSWER There is no MII when comparing one OS to continuous with oocyte aspiration. WHAT IS KNOWN ALREADY Based on concept multiple follicular waves, combination two stimulations same cycle has gained interest patients low reserve. This so-called dual approach usually characterized by discontinuation FSH...
Poliovirus receptor (Pvr), erythrocyte protein band 4.1-like 3 (Epb4.1l3), regulator of G-protein signaling 11 (Rgs11), and oxytocin (Oxtr) expression were quantified in vitro- vivo-grown mouse follicles. The all genes was increased during antral growth vitro-grown cumulus cells, whereas only Rgs11 Oxtr Pvr Epb4.1l3 decreased vivo grown cells. In mural granulosa cells showed the highest Pvr, Rgs11, Oxtr. vitro + theca compartment responded to human chorionic gonadotropinduring early...