A5112117622- Viral Infections and Immunology Research
- COVID-19 Clinical Research Studies
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Long-Term Effects of COVID-19
- Cardiac electrophysiology and arrhythmias
- RNA regulation and disease
- Cytomegalovirus and herpesvirus research
- Genomics and Phylogenetic Studies
- COVID-19 and healthcare impacts
- Cardiovascular Effects of Exercise
- Posttraumatic Stress Disorder Research
- Tryptophan and brain disorders
- S100 Proteins and Annexins
- Demographic Trends and Gender Preferences
- Cardiac Fibrosis and Remodeling
- Immune responses and vaccinations
- Salmonella and Campylobacter epidemiology
- Single-cell and spatial transcriptomics
- Liver Disease Diagnosis and Treatment
- Insect behavior and control techniques
- Healthcare Systems and Reforms
- Probiotics and Fermented Foods
- Global Maternal and Child Health
- Neurogenesis and neuroplasticity mechanisms
Fifth Affiliated Hospital of Zhengzhou University
2015-2025
China National Research Institute of Food and Fermentation
2024-2025
GTx (United States)
2021
Icahn School of Medicine at Mount Sinai
2018
Zhengzhou University
2014
Rockefeller University
2012
Zhengzhou Central Hospital
2005
Purpose The childhood hand, foot and mouth disease (HFMD) sequelae cohort study (HNHFMDCS) is an ambispective of patients with HFMD based in Henan Province, China, consisting treated a key hospital for the diagnosis treatment Province. aims to investigate long-term survivors provide comprehensive understanding potential harm caused by this infectious disease. Participants In retrospective phase study, children diagnosed from January 2014 2023 were included, clinical demographic information...
Background Large-scale outbreaks of hand-foot-mouth disease (HFMD) have occurred frequently and caused neurological sequelae in mainland China since 2008. Prediction the activity HFMD epidemics a few weeks ahead is useful taking preventive measures for efficient control. Methods Samples obtained from children hospitalized with Zhengzhou, Henan, China, were examined existence pathogens reverse-transcriptase polymerase chain reaction (RT-PCR) 2008 to 2012. Seasonal Autoregressive Integrated...
The identification and quantification of viable bacteria at the species/strain level in compound probiotic products is challenging now. Molecular biology methods, e.g., propidium monoazide (PMA) combination with qPCR, have gained prominence for targeted cell counts. This study endeavors to establish a robust PMA-qPCR method Lacticaseibacillus rhamnosus detection systematically validated key metrics encompassing relative trueness, accuracy, limit quantification, linear, range. inclusivity...
Streptococcus thermophilus is widely used as a starter culture in the production of cheese, yoghurt and various cultured dairy products, which holds considerable significance both research practical applications within food industry. Throughout history, taxonomy S. has undergone several adjustments revisions. In 1984, based on result DNA–DNA hybridization, was reclassified salivarius subsp. . Subsequently, 1991, Schleifer et al proposed revival species also relying hybridization study...
Background Hand-foot-and-mouth disease_(HFMD) is one of the most typical diseases in children that associated with high morbidity. Reliable forecasting crucial for prevention and control. Recently, hybrid models have become popular, wavelet analysis has been widely performed. Better prediction accuracy may be achieved using wavelet-based models. Thus, our aim to forecast number HFMD cases Materials methods We fitted a seasonal autoregressive integrated moving average (SARIMA)–neural network...
Enterovirus (EV) infection has been a serious health issue in Asia-Pacific region. It indicated that the occurrence of fatal hand foot and mouth disease (HFMD) cases following EV71 is mainly attributed to pulmonary edema. However, development disorders after remains largely unknown. To establish an EV71-infected animal model further explore underlying association central nervous system (CNS) invasion with edema, we isolated clinical source strain (ZZ1350) from severe case Henan Province.We...
In order to train more high-level general practitioners (GPs) work in primary care institutions, China launched the 5+3 model 2015 as a way educate GPs nationwide. this study, we investigated awareness of model, career choices after graduation, and influences on GP choice undergraduate medical students from Zhengzhou University. The study population consisted 288 We explored students׳ practitioner mental status by using self-report questionnaire Chinese version 21-item Depression Anxiety...
Background We investigated trans‐generational associations between Post Traumatic Stress Disorder (PTSD) symptoms in World Trade Center (WTC) responders and behavioral problems their children. Methods Participants were WTC responders—8034 police 8352 non‐traditional (eg, construction workers)—with one or more children at the time of first visit to Health Program (WTC‐HP). Self‐report questionnaires administered approximately 4 years after 9/11 attack. Results A total 31.4% 20.0% reported...
Hand, foot and mouth disease (HFMD) is an infectious that affects mostly children. The children with HFMD also have other immune metabolic disorders. However, the association of these disorders severity has not yet been determined. In this study, we used a case-control study design to examine correlation development in 406 mild severe patients were recruited divided into different subgroups based on number days from initial onset time hospitalization (1, 2, 3, 4, ≥5 days). Logistic...
Hand, foot, and mouth disease (HFMD) is a common childhood infectious caused by variety of enteroviruses (EVs). To explore the epidemiological characteristics etiology HFMD in Zhengzhou, China, we conducted systematic analysis surveillance data from Zhengzhou Center for Disease Control Prevention January 2009 to December 2021 (https://wjw.zhengzhou.gov.cn/). Surveillance were collected Cases analyzed according time onset, type diagnosis, characteristics, viral serotype, trends. We found that...
A major barrier to complex experimental design in mouse genetics is the allele problem: combining three or more alleles time-consuming and inefficient. Here, we solve this problem for transgenic animals with a simple modification of existing BAC transgenesis protocols, generate triple-colored ‘prism’ mice which cell types brain: neurons, astrocytes, oligodendrocytes, are each labeled distinct fluorophore. All fluorophores expressed from same locus, yet fluorophore an independent temporal...
Abstract Coxsackievirus A6 (CVA6) is currently considered as a predominant pathogen of hand, foot, and mouth disease (HFMD), occasionally linked to myocardial injury. We first established mouse model CVA6-induced Next, we analyzed the immune cell phenotypes CVA6-infected mice hearts by fluorescence-activated sorting, found that CVA6 led massive neutrophils infiltration, suggesting their potential link with occurrence further used either αGr-1 or αLy6G antibody deplete neutrophils,...
In recent years, the prevalence of hand-foot-mouth disease (HFMD) in China and some other countries has caused worldwide concern. Mild cases tend to recover within a week, while severe may progress rapidly have bad outcome. Since there is no vaccine for HFMD anti-inflammatory treatment not ideal. this study, we aimed establish valid forecasting model using common laboratory parameters.Retrospectively, 77 from Zhengzhou Children's hospital peaking period between years 2013 2015 were...
Abstract Background: Hand-foot-and-mouth disease(HFMD) is one of the most common diseases in children, which has high morbidity. Reliable forecasting significant for prevention and control. Recently, hybrid models have been becoming popular wavelet analysis widely used. Better prediction accuracy may be achieved with wavelet-based models. Thus, our aim to forecast number HFMD cases Materials methods: We fitted a SARIMA(seasonal autoregressive integrated moving average)-NNAR(neural network...
A Gram-stain-positive, strictly anaerobic, endospore-forming and rod-shaped (0.6-0.8×2.7-13.1 µm) bacterium, designated as 5 N-1
Coxsackievirus (CV) A6 has emerged as an important causative agent in global outbreaks of hand, foot, and mouth disease (HFMD), which typically presents a mild illness with large generalized rash, herpes. However, some patients can develop encephalitis, pneumonia, myocarditis liver injury. Our previous study took the view that CVA6 could replicate mouse liver, leading to acute injury; however, precise underlying mechanism remains elusive. 10-day-old wild-type (WT, C57BL/6J) NLRP3 knock-out...
Investigating the causal relationship between circulating immune cells, blood metabolites, and severe COVID-19 revealing role of metabolite-mediated cells in disease onset progression. Genetic variation data 731 1400 from genome-wide association study open-access database (https://gwas.mrcieu.ac.uk) were used as instrumental variables for bidirectional two-step Mendelian randomization analysis. The identified 11 with unidirectional causality to COVID-19. Two-step analysis showed 10...
ABSTRACT Hand, foot, and mouth disease (HFMD) is a serious pediatric infectious that causes immeasurable physical mental health burdens. Currently, there lack of information on the mechanisms HFMD severity early diagnosis. We performed metabolomic profiling sera from 84 Enterovirus A71 (EV-A71) infections 45 control individuals. Targeted metabolomics assays were employed to further validate some differential metabolic molecules. identified significant molecular changes in patients compared...
COVID-19 is a global epidemic caused by severe acute respiratory syndrome coronavirus 2 infection. However, the understanding of long-term diseases in survivors remains limited.To identify incidence and risk factors post-COVID-19 pulmonary fibrosis, comprehensive search relevant studies published before 2nd April 2023 PubMed, Embase, Web Science, Cochrane Library was conducted, 18 papers were eligible for this study. The existing literature evidence showed that about 36.4% may have developed...