A5082125789- Antibiotic Resistance in Bacteria
- Enterobacteriaceae and Cronobacter Research
- Viral gastroenteritis research and epidemiology
- Clostridium difficile and Clostridium perfringens research
- Antibiotic Use and Resistance
- Salmonella and Campylobacter epidemiology
- Nosocomial Infections in ICU
- Respiratory viral infections research
- Bacterial Identification and Susceptibility Testing
- Antibiotics Pharmacokinetics and Efficacy
- Viral Infections and Immunology Research
- Vibrio bacteria research studies
- Urinary Tract Infections Management
- Escherichia coli research studies
- Pneumonia and Respiratory Infections
- Bacterial biofilms and quorum sensing
- SARS-CoV-2 and COVID-19 Research
- Toxin Mechanisms and Immunotoxins
- COVID-19 and healthcare impacts
- Parasitic Infections and Diagnostics
- Influenza Virus Research Studies
- Streptococcal Infections and Treatments
- Infections and bacterial resistance
- Virus-based gene therapy research
- Pharmaceutical and Antibiotic Environmental Impacts
Hospital Universitario La Paz
2016-2025
Instituto de Salud Carlos III
2005-2025
Centro de Investigación Biomédica en Red
2023-2025
Hospital La Paz Institute for Health Research
2012-2025
Centre for Biomedical Network Research on Rare Diseases
2024
Laboratoire des Sciences de l'Ingénieur, de l'Informatique et de l'Imagerie
2022
Southwest College of Naturopathic Medicine & Health Sciences
2016-2021
Urology Foundation
2021
NIHR Birmingham Biomedical Research Centre
2021
University of Birmingham
2021
ABSTRACT The aim of this study was to determine the impact carbapenemase-producing Enterobacteriaceae (CPE) in Spain 2013 by describing prevalence, dissemination, and geographic distribution CPE clones, their population structure antibiotic susceptibility. From February May 2013, 83 hospitals (about 40,000 hospital beds) prospectively collected nonduplicate using screening cutoff recommended EUCAST. Carbapenemase characterization performed phenotypic methods confirmed PCR sequencing....
CARB-ES-19 is a comprehensive, multicenter, nationwide study integrating whole-genome sequencing (WGS) in the surveillance of carbapenemase-producing K. pneumoniae (CP-Kpn) and E. coli (CP-Eco) to determine their incidence, geographical distribution, phylogeny, resistance mechanisms Spain.In total, 71 hospitals, representing all 50 Spanish provinces, collected first 10 isolates per hospital (February May 2019); CPE were identified according EUCAST (meropenem MIC > 0.12 mg/L with...
Bat coronaviruses (CoV) are putative precursors of the severe acute respiratory syndrome (SARS) CoV and other that crossed species barrier from zoonotic reservoirs into human population. To determine presence distribution in Iberian bats, 576 individuals 26 different bat were captured 13 locations Spain. We report for first time 14 9 species. Phylogenetic analysis a conserved genome region (RdRp gene) shows wide diversity alpha betacoronavirus Interestingly, although some these viruses...
ObjectivesWe describe clinical and microbiological features of infections caused by OXA-48-producing Klebsiella pneumoniae (O48KP) in the setting a prolonged, hospital-wide outbreak detected January 2011.
Abstract Objectives NDM carbapenemases have spread worldwide. However, little information exists about the impact of NDM-producing Enterobacteriaceae in Spain. By WGS, we sought to elucidate population structure NDM-like-producing Klebsiella pneumoniae and Escherichia coli Spain determine plasmids harbouring blaNDM-like genes. Methods High-resolution SNP typing, core-genome MLST plasmid reconstruction (PlasmidID) were performed on 59 K. 8 E. isolated over an year period Results Five major...
Abstract Background Carbapenem-resistant Enterobacteriaceae (CRE) are an emerging problem in the paediatric population worldwide with high mortality rates bloodstream infection (BSI). Objectives To evaluate predictors of 30 day CRE BSI a cohort. Methods A retrospective observational single-centre study (December 2005–August 2018) was conducted. Cases children 0 to 16 years were included. Microbiological identification (MALDI Biotyper) and antimicrobial susceptibility testing (Vitek2®...
The aim is determining the impact of non-pharmaceutical measures (NPIs) against SARS-CoV-2 in incidence and prevalence gastrointestinal viruses (GV) children. Demographic, analytical, clinical data children from which samples were received at Hospital Universitario La Paz (Madrid, Spain) that had a infection with positive sample through multiplex-PCR for GV collected. time periods included prepandemic (P1): March 14, 2019 to 2020 pandemic (P2): 15, 2021. global prevalence, relative (RI, per...
Objetive: The aim of this study was to describe the impact non-pharmaceutical interventions (NPIs) against SARS-CoV-2 in patients with symptoms enteric protozoa (EP), including Blastocystis spp., Dientamoeba fragilis, Giardia lamblia, Cryptosporidium Entamoeba histolytica, and Cyclospora cayetanensis, overall population who were consulted at a National Referral Center for Imported Tropical Diseases (NRCITD patients) from healthcare area Madrid (Spain). Method: Data on positive RT-PCR results...
Abstract Background Community-acquired (CA) and healthcare-associated (HCA) infections caused by carbapenemase-producing Enterobacterales (CPE) are not well characterized. The objective was to provide detailed information about the clinical molecular epidemiological features of nosocomial, HCA CA Klebsiella pneumoniae (CP-Kp) Escherichia coli (CP-Ec). Methods A prospective cohort study performed in 59 Spanish hospitals from February March 2019, including first 10 consecutive patients whom...
There is a critical need for rapid diagnostic methods multidrug-resistant (MDR) pathogens in patients with suspicion of ventilator-associated pneumonia (VAP). The Xpert Carba-R detects 5 targets carbapenemase-producing organisms (blaKPC, blaNDM, blaVIM, blaOXA-48, and blaIMP-1). Our objective was to evaluate the performance this assay directly on bronchial aspirates correlate cycle number positive result (Ct) bacterial count. Bronchial from VAP were spiked dilution 1 4 MDR carrying...
In the context of long-term screening for viruses on Western Palaearctic bats, we tested presence adenovirus 1392 oropharyngeal swabs and 325 stool samples taken from 27 bat species. Adenoviruses were detected in 12 species Vespertilionidae Rhinolophidae families. Fifty positive respiratory 26 studied. Phylogenetic analyses partial hexon protein DNA-dependent DNA polymerase genes indicate that all these adenoviruses belong to genus Mastadenovirus but without constituting a monophyletic...
In this study, we determined the presence of virulence factors in nonoutbreak, high-risk clones and other isolates belonging to less common sequence types associated with spread OXA-48-producing Klebsiella pneumoniae clinical from The Netherlands (n = 61) Spain 53). Most shared a chromosomally encoded core factors, including enterobactin gene cluster, fimbrial fim mrk clusters, urea metabolism genes (ureAD). We observed high diversity K-Locus K/O loci combinations, KL17 KL24 (both 16%),...
In 2022, the United Kingdom reported an increase in drug resistance Shigella sonnei isolates. We report 33 cases Spain genetically related to UK and 4 with similar antimicrobial profiles infected distant strains. Our results suggest circulation of multiple genetic clusters multidrug-resistant S. Spain.
ABSTRACT Clinical research suggests a role for viral load measurement in predicting and monitoring Epstein-Barr virus (EBV)-associated diseases. The aim of this study was to assess the performance recently commercially available quantitative assays EBV based on real-time PCR: RealArt LC PCR kit LightCycler quantification kit. A total 87 samples were analyzed: 67 obtained from transplant recipients patients with EBV-associated diseases, 8 Quality Control Molecular Diagnostics 2002 Proficiency...