A5003838811- Medical Imaging Techniques and Applications
- Advanced X-ray and CT Imaging
- Advanced X-ray Imaging Techniques
- Digital Imaging for Blood Diseases
- Advancements in Photolithography Techniques
- Electron and X-Ray Spectroscopy Techniques
- Medical Image Segmentation Techniques
- Integrated Circuits and Semiconductor Failure Analysis
- Microplastics and Plastic Pollution
- Cell Image Analysis Techniques
- Advanced MRI Techniques and Applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Radiation Shielding Materials Analysis
- Immune cells in cancer
- Immune Response and Inflammation
- Radiomics and Machine Learning in Medical Imaging
- Paleontology and Stratigraphy of Fossils
- Ocular Infections and Treatments
- Polymer Surface Interaction Studies
- Caveolin-1 and cellular processes
- Nanomaterials for catalytic reactions
- Chronic Kidney Disease and Diabetes
- Inflammasome and immune disorders
- Autoimmune and Inflammatory Disorders Research
- Bone Tissue Engineering Materials
Fraunhofer Institute for Ceramic Technologies and Systems
2021-2022
Max Planck Institute for the Science of Light
2018-2021
Friedrich-Alexander-Universität Erlangen-Nürnberg
2021
Freie Universität Berlin
2021
Luxembourg Institute of Science and Technology
2021
Flemish Institute for Technological Research
2021
Helmholtz-Zentrum Berlin für Materialien und Energie
2019
Papillon-Lefèvre syndrome (PLS) is characterized by nonfunctional neutrophil serine proteases (NSPs) and fulminant periodontal inflammation of unknown cause. Here we investigated extracellular trap (NET)-associated aggregation cytokine/chemokine-release/degradation normal NSP-deficient human mouse granulocytes. Stimulated with solid or soluble NET inducers, neutrophils formed aggregates both released degraded cytokines/chemokines. With increasing cell density, proteolytic degradation...
Abstract Eye rheum is a physiological discharge, which accumulates at the medial angle of healthy eye soon after opening in morning. Microscopic evaluation revealed presence viable neutrophils, bacteria, epithelial cells, and particles, aggregated by neutrophil extracellular traps. We observed that evening, during closure, high C5a recruited neutrophils to tear film activated them. In this hypoxic area rich CO2, fight microbial aggressors degranulation. Immediately opening, microenvironment...
Nanoparticles occur in various environments as a consequence of man-made processes, which raises concerns about their impact on the environment and human health. To allow for proper risk assessment, precise statistically relevant analysis particle characteristics (such size, shape, composition) is required that would greatly benefit from automated image procedures. While deep learning shows impressive results object detection tasks, its applicability limited by amount representative,...
Abstract The creation of bioartificial tissues is a promising option for the reconstruction large‐volume defects. vascularization tissue engineering constructs, as well material properties carrier matrix, are important factors successful clinical application. In this regard, hydrogels biomaterials, providing an extracellular matrix‐like milieu that enables possibility cell transplantation and de novo formation. Furthermore, biofunctionalization allows certain fine‐tuning angiogenic...
Microplastics (MPs) have been reported in various environmental compartments and their number is continuously increasing because of degradation into smaller fragments down to nanoplastics. Humans are exposed these small-sized MPs through food air with potential health consequences that still need be determined. This requires, the first place, efficient detailed visualization, relocalization, characterization same complementary analytical methods. Here, we show application a correlative...
Key features are presented of two high-resolution EUV imaging tools: the MS-13 Microstepper wafer exposure and RIM-13 reticle microscope. The has been developed for resist testing technology evaluation at 32nm node beyond, while is designed actinic aerial image monitoring blank patterned reticles. Details design architecture, module layout, major subsystems performance both tools.
Podocytes are critical components of the glomerular filtration barrier, sitting on outside basement membrane. Primary and secondary foot processes characteristic for podocytes, but cell that develop in culture were not studied much past. Moreover, protocols diverse visualization methods mostly can only be used one technique, due to differences fixation, drying handling. However, we detected by single-cell RNA sequencing (scRNAseq) analysis cells reveal high variability genes involved...
Hollow mesoporous silica capsules (HMSC) are potential drug transport vehicles due to their biocompatibility, high loading capacity and sufficient stability in biological milieu. Herein, we report the synthesis of ellipsoid-shaped HMSC (aspect ratio ∼2) performed using hematite particles as solid templates that were coated with a conformal shell through cross-condensation reactions. For obtaining hollow capsules, iron oxide core was removed by acidic leaching. Gas sorption studies on...
High-resolution X-ray microscopy (XRM) is gaining interest for biological investigations of extremely small-scale structures. XRM imaging bones in living mice could provide new insights into the emergence and treatment osteoporosis by observing osteocyte lacunae, which are holes bone few micrometres size. Imaging animals at that resolution, however, challenging requires very sophisticated data processing converting raw detector output reconstructed images. This paper presents an open-source,...
Abstract Through silicon vias (TSVs) are a key enabling technology for interconnection and realization of complex three‐dimensional integrated circuit (3D‐IC) components. In order to perform failure analysis without the need destructive sample preparation, x‐ray microscopy (XRM) is rising method analyzing internal structure samples. However, there still lack evaluated scan recipes or best practices regarding XRM parameter settings study TSVs in current state literature. There also an...
<h3>Background:</h3> In ageing societies all over the globe, number of people suffering from bone disease, e.g. osteoporosis (OP) in first place, has increased dramatically. OP considerably impairs patients' life quality, and results high societal costs. However, current understanding is still insufficient due to lack appropriate resolution tools that permit a thorough analysis scale bridging architectures macro nano with statistical significance support development better treatments by...
Abstract Background and Aims Despite increasing use of plastic based products their potential health risks on the humans, very little is known about possible accumulation in food chain further long-term effect human health. Recently, there are reports related to risk polystyrene microplastics respiratory system intestinal epithelia cell line. In this study, we assayed primary microplastic particles kidney cells. To that aim, used podocytes cells four different types including; polyvinyl...
Key features of the RIM-13 EUV actinic reticle imaging microscope are summarised. This is a tool which generates aerial images from blank or patterned masks, emulating illumination and projection optics an exposure tool. Such mask defects, acquired by CCD camera, analysed using software to predict their effect on resist exposure. Optical, mechanical performance reported.
Reconstruction of large volume defects with autologous tissue may be limited by the associated donor site morbidity. The generation bioartificial replacement tissues according to principles engineering represents a promising possibility for reduction vascularization constructs is an important parameter successful clinical application. Surgically induced angiogenesis using arteriovenous (AV) loop can vascularize biomaterials. In addition vascularization, material properties carrier matrix...
Key features are presented of the RIM-13 reticle imaging microscope developed for actinic aerial image monitoring blank and patterned EUV reticles. Details opto-mechanical design, module layout, major subsystems including source performance tool presented.