A5100780297- Prenatal Screening and Diagnostics
- Genomic variations and chromosomal abnormalities
- Reproductive Biology and Fertility
- Cardiac electrophysiology and arrhythmias
- Sperm and Testicular Function
- Genetic Syndromes and Imprinting
- Fetal and Pediatric Neurological Disorders
- Molecular Biology Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Assisted Reproductive Technology and Twin Pregnancy
- Ion channel regulation and function
- Reproductive System and Pregnancy
- Cancer Genomics and Diagnostics
- Atrial Fibrillation Management and Outcomes
- Genomics and Rare Diseases
- Renal and related cancers
- Ovarian function and disorders
- Cardiovascular Effects of Exercise
- Receptor Mechanisms and Signaling
- Congenital heart defects research
- Antiplatelet Therapy and Cardiovascular Diseases
- Cardiac Imaging and Diagnostics
- Soft Robotics and Applications
- Cardiomyopathy and Myosin Studies
- Regional Development and Environment
Shanghai Jiao Tong University
2016-2025
Southwest Minzu University
2024-2025
Chongqing Medical University
2024
State Key Laboratory of Microbial Metabolism
2024
Huazhong University of Science and Technology
2020
Union Hospital
2020
Philips (United States)
2020
Technical Solutions (United States)
2020
State Key Laboratory of Information Engineering in Surveying Mapping and Remote Sensing
2020
Taihe Hospital
2019
This study examined the role of CRH-induced ovarian cell apoptosis in restraint stress (RS)-induced impairment oocyte competence. Oocyte percentages apoptotic cumulus cells (CCs) did not differ between stressed and control mice before vitro maturation (IVM) but became significantly higher after IVM without serum, growth factor, hormone. The level Bcl2 mRNA decreased mural granulosa (MGCs) homogenates RS. Whereas estradiol, testosterone, IGF1 decreased, cortisol progesterone increased...
Type 2 diabetes is associated with abnormal electrical conduction and sudden cardiac death, but the pathogenic mechanism remains unknown. This study describes electrophysiological alterations in a diet-induced pre-diabetic rat model examines underlying mechanism. Sprague–Dawley rats were fed either high-fat diet fructose water or normal chow for 6 weeks. The properties of whole heart was analyzed by vivo surface ECG recordings, as wells ex Langendorff perfused hearts during baseline,...
Objectives At present, most genetic tests or carrier screening are performed with blood samples, and the known rate of disease-causing variants is also derived from blood. For semen donors, what really passed on to offspring pathogenic variant in their sperm. This study aimed determine whether identified sperm young donors present blood, matching results for consistent
As a significant component of China's historical architectural heritage, traditional dwellings in the Liangshan region offer wealth ecological construction experience. However, due to age-old limitations techniques, indoor thermal environment these during winter is salient issue. This paper investigates current status using multifaceted research method that incorporates Climate Consultant climate analysis software, on-site measurements, numerical simulation and theoretical analysis. The...
Despite the gradual application of third-generation long read sequencing (LRS) or reference embryo establishment to preimplantation genetic testing for structural rearrangement (PGT-SR) without familial involvement, there are still limitations their extensive clinical yet. This study developed a novel virtual NGS-based method (ViLR) and preliminarily evaluated its feasibility breakpoint characterization direct SNP haplotyping de novo chromosomal rearrangements (CSR). A total 10 families with...
This electronic investigates the wind environment optimization of office build-ing clusters in hot-summer and cold-winter regions through computation-al fluid dynamics simulations under dual-season conditions. A three-dimensional nu-merical model encompassing a 1200-meter extended area was established, em-ploying high-precision meshing advanced turbulence models to analyze speed distribution at pedestrian height winter northwest prevailing winds summer southeast wind-induced surface...
Abstract STUDY QUESTION Is there an efficient and cost-effective detection platform for different genetic conditions about embryos? SUMMARY ANSWER A multifunctional haplotyping-based preimplantation testing was provided detecting conditions. WHAT IS KNOWN ALREADY Genetic disease chromosomal rearrangement have been known to significantly impact fertility development. Therefore, aneuploidy (PGT-A), monogenic disorders (PGT-M) structural rearrangements (PGT-SR), a part of ART, has presented...
Measurement of cell-free fetal DNA (cffDNA) is an indispensable process for non-invasive prenatal screening (NIPS). According to recent studies, cffDNA in maternal plasma can be enriched various lengths fragments, and a sufficient amount effectively eliminate background interference on the part DNA. Therefore, we developed simple effective separation method, improved NIPS (iNIPS), that enriches fraction improves accuracy aneuploid detection. We adopted novel strategy achieve enrichment...
Manual semen evaluation methods are subjective and time-consuming. In this study, a deep learning algorithmic framework was designed to enable non-invasive multidimensional morphological analysis of live sperm in motion, improve current clinical morphology testing methods, significantly contribute the advancement assisted reproductive technologies. We improved FairMOT tracking algorithm by incorporating distance angle same head movement adjacent frames, as well target detection frame IOU...
The non-invasive and rapid assessment of the developmental potential embryos is great clinical importance in assisted reproductive technology (ART). In this retrospective study, we analyzed metabolomics 107 samples provided by volunteers utilized Raman spectroscopy to detect substance composition discarded culture medium 53 resulting successful pregnancies 54 that did not result pregnancy after implantation. from D3 cleavage-stage was collected transplantation a total 535 (107 × 5) original...
It is known that oocytes and cumulus cells (CCs) are more resistant to apoptosis than other compartments of the antral follicle. However, although oocyte-secreted factors (OSFs) have been found be involved in suppressing bovine CC apoptosis, little about intracellular mechanisms by which OSFs render CCs apoptosis. Here, we show coculture with mouse or pig cumulus-denuded oocytes, culture recombinant growth differentiation factor-9 (GDF-9), oocyte-conditioned medium (POCM) significantly...
A recent experimental study investigating patients with lone atrial fibrillation identified six novel mutations in the KCNA5 gene. The mutants exhibited both gain- and loss-of-function of specific ultra-rapid delayed rectifier K+ current, IKur. aim this is to elucidate quantify functional impact these on electrical activity. multi-scale model human atria was updated incorporate detailed data IKur from wild-type mutants. effects action potential rate dependence were investigated at cellular...
Massively parallel sequencing (MPS) combined with bioinformatic analysis has been widely applied to detect fetal chromosomal aneuploidies such as trisomy 21, 18, 13 and sex chromosome (SCAs) by cell-free DNA (cffDNA) from maternal plasma, so-called non-invasive prenatal testing (NIPT). However, many technical challenges, dependency on correct prediction, large variations of Y measurement high sensitivity random reads mapping, may result in higher false negative rate (FNR) positive (FPR)...
With the development and clinical application of genomics, more concern is focused on single-cell sequencing. In process sequencing, whole genome amplification a key step to enrich sample DNA. Previous studies have compared performance different (WGA) strategies Illumina sequencing platforms, but there no related research aimed at Ion Proton platform, which also popular next-generation platform. Here by amplifying cells from six cell lines with karyotypes, we estimated data features four...
This study was aimed to report the clinical characteristics of fetal chromosomal aneuploidy diseases using noninvasive prenatal testing (NIPT) in twin pregnancies and analyze results terms chorionicity, conception, fraction.A total 1160 women with were recruited from 1 October 2015, August 2017. Next-generation sequencing technology used detect aneuploidies, such as trisomy 21, 18, 13 X.Aneuploidy detected NIPT 26 fetuses, among which 18 aneuploidies occurred only one fetus twins. The rate...
G protein-coupled inwardly rectifying K⁺ channels (GIRK) are important in the regulation of heart rate and atrial electrophysiology. GIRK activated by receptors, including muscarinic M₂ receptors adenosine A₁ receptors. The aim this study was to characterize compare electrophysiological effects acetylcholine (ACh) on rat atria. Action potential duration at 90% repolarization (APD₉₀), effective refractory period (ERP), resting membrane (RMP) were investigated isolated atria intracellular...