A5050846205- Ion channel regulation and function
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Anorectal Disease Treatments and Outcomes
- Pelvic floor disorders treatments
- Platelet Disorders and Treatments
- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Ion Channels and Receptors
- Electrospun Nanofibers in Biomedical Applications
- Neuroscience and Neuropharmacology Research
- Cardiac Structural Anomalies and Repair
- Osteoarthritis Treatment and Mechanisms
- Protein Kinase Regulation and GTPase Signaling
- Corneal Surgery and Treatments
- Diverticular Disease and Complications
- Receptor Mechanisms and Signaling
- Robotic Path Planning Algorithms
- Colorectal Cancer Surgical Treatments
- Mechanical Circulatory Support Devices
- Cardiac electrophysiology and arrhythmias
- Nitric Oxide and Endothelin Effects
- EEG and Brain-Computer Interfaces
- Robotics and Sensor-Based Localization
- Transplantation: Methods and Outcomes
Universidad Complutense de Madrid
2017-2025
Instituto de Biomedicina y Genética Molecular de Valladolid
2013-2024
Citospin (Spain)
2024
Institut Català d'Oncologia
2024
Universitat de Barcelona
2024
Hospital de Basurto
2024
Institut Català d'Ornitologia
2024
Institut d'Investigació Biomédica de Bellvitge
2024
Universidad de Valladolid
2012-2021
Consejo Superior de Investigaciones Científicas
1996-2021
Osteoarthritis is the most prevalent joint disease and a common cause of pain, functional loss, disability. Conventional treatments demonstrate only modest clinical benefits without lesion reversal. Autologous mesenchymal stromal cell (MSC) have shown feasibility, safety, strong indications for efficacy. We performed randomized, active control trial to assess feasibility safety treating osteoarthritis with allogeneic MSCs, we obtain information regarding efficacy this treatment.We randomized...
Bone marrow mononuclear cells (BMCs) from 20 patients with extensive reperfused myocardial infarction (MI) were used to assess their regenerative capability “in vitro” and effect on postinfarction left ventricular (LV) remodeling. Human BMCs labeled, seeded top of cryoinjured mice heart slices, cultured. showed tropism for ability graft into the damaged mouse cardiac tissue and, after 1 week, acquired a cardiomyocyte phenotype expressed proteins, including connexin43. In clinical trial,...
Degenerative disc disease may cause severe low-back pain, a large public health problem with significant economic and life quality impact. Chronic cases often require surgery, which lead to biomechanical problems accelerated degeneration of the adjacent segments. Cell-based therapies circumvent these have exhibited encouraging results in vitro animal studies. We designed pilot study assess feasibility safety obtain early indications on efficacy treatment mesenchymal stem cells (MSC)...
In Brief Background Osteoarthritis is the most prevalent joint disease and a frequent cause of pain, functional loss, disability. often becomes chronic, conventional treatments have demonstrated only modest clinical benefits without lesion reversal. Cell-based therapies shown encouraging results in both animal studies few human case reports. We designed pilot study to assess feasibility safety osteoarthritis treatment with mesenchymal stromal cells (MSCs) humans obtain early efficacy...
In Brief Background Degenerative disc disease often causes severe low-back pain, a public health problem with huge economic and life quality impact. Chronic cases require surgery, which may lead to biomechanical problems accelerated degeneration of the adjacent segments. Autologous mesenchymal stromal cells (MSC) treatments have shown feasibility, safety strong indications clinical efficacy. We present here randomized, controlled trial using allogeneic MSC, are logistically more convenient...
The role of changes in cytoplasmic free calcium, [Ca2+]i, the responses to platelet-activating factor (PAF) was studied human platelets loaded with fluorescent calcium indicator, quin2. In presence 1 mM external PAF raised [Ca2+]i 8-10-fold a few seconds peak near microM. then declined over several minutes towards basal level. absence there much smaller increase similar pattern. These findings suggest that increases partly by discharge internal Ca2+, but mainly stimulated influx. Blockade...
Objective . To evaluate outcomes of cultivated limbal epithelial transplantation (CLET) for management ocular surface failure due to stem cell deficiency (LSCD). Design Prospective, noncomparative, interventional case series and extensive comparison with recent similar studies. Participants Twenty eyes LSCD underwent CLET (11 autologous; 9 allogeneic) were followed up 3 years. Etiologies divided into prognostic categories: Group 1, chemical injuries (7 eyes); 2, immune-based inflammation (4...
Animal and human studies have suggested the potential of mesenchymal stromal cells (MSCs) to treat systemic lupus erythematosus (SLE). Here, we present results compassionate MSC treatments for three SLE patients provide proof concept a randomized controlled clinical trial. Three different ethnicities who suffer from chronic SLE, presented with class IV active proliferative nephritis confirmed by biopsy, were treated allogeneic MSCs healthy donors. Ninety million infused intravenously into...
We have studied the relation between filling state of intracellular Ca2+ stores and plasma-membrane permeability to Mn2+, used here as a surrogate for channels. Emptying either by incubation in Ca(2+)-free medium or treatment with low concentrations ionophore ionomycin accelerated influx Mn2+. Refilling Ca(2+)-containing restores Mn2+ permeability. This Ca(2+)-store-regulated was inhibited Ni2+ cytochrome P-450 inhibitors. Stimulation platelets thrombin produced release from stores, which...
<h3>Objectives</h3> To assess the efficacy of a single intradiscal injection allogeneic bone marrow mesenchymal stromal cells (BM-MSCs) versus sham placebo in patients with chronic low back pain (LBP). <h3>Methods</h3> Participants were randomised prospective, double-blind, controlled study to receive either or 20 million BM-MSC, between April 2018 and December 2022. The first co-primary endpoint was rate responders defined by improvement Visual Analogue Scale (VAS) for at least 20% mm,...
The necessity for more effective therapies chronic osteoarticular diseases has led to the development of treatments based on mesenchymal stem cells (MSCs), natural precursors musculoskeletal tissue. Treatments with autologous MSCs yielded excellent results, nearly 70% improvement pain and disability in osteoarthritis degenerative disc disease. Using allogeneic is logistically convenient would widen pool eligible patients, but potential immune rejection should be considered. In this context,...
The classic view for hypothalamic regulation of anterior pituitary (AP) hormone secretion holds that release each AP is controlled specifically by a corresponding hypothalamic-releasing (HRH). In this scenario, binding given HRH (thyrotropin-, growth hormone-, corticotropin-, and luteinizing hormone-releasing hormones) to specific receptors in its target cell increases the concentration cytosolic Ca 2+ ([Ca ] i ), thereby selectively stimulating appropriate hormone. However, “paradoxical”...
The aim of this study was to evaluate injectable, in situ cross-linkable elastin-like recombinamers (ELRs) for osteochondral repair. Both the ELR-based hydrogel alone and embedded with rabbit mesenchymal stromal cells (rMSCs) were tested regeneration critical subchondral defects 10 New Zealand rabbits. Thus, cylindrical filled an aqueous solution ELRs animals sacrificed at 4 months histological gross evaluation features biomaterial performance, including integration, cellular infiltration,...