A5085046409- Immune cells in cancer
- Immune Cell Function and Interaction
- Liver Disease Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Phagocytosis and Immune Regulation
- Peroxisome Proliferator-Activated Receptors
- Galectins and Cancer Biology
- Drug Transport and Resistance Mechanisms
- Chemotherapy-related skin toxicity
- Liver physiology and pathology
- Drug-Induced Adverse Reactions
- Nanoplatforms for cancer theranostics
- Monoclonal and Polyclonal Antibodies Research
- Endoplasmic Reticulum Stress and Disease
- Cancer, Lipids, and Metabolism
- Nanoparticle-Based Drug Delivery
- Cholesterol and Lipid Metabolism
- Mesenchymal stem cell research
- Extracellular vesicles in disease
- Lung Cancer Treatments and Mutations
- Biomarkers in Disease Mechanisms
- Cardiovascular Function and Risk Factors
- Autophagy in Disease and Therapy
- Microfluidic and Bio-sensing Technologies
- Diabetes, Cardiovascular Risks, and Lipoproteins
Instituto de Investigación Sanitaria de Santiago
2024-2025
Center for Research in Molecular Medicine and Chronic Diseases
2022-2024
Universidade de Santiago de Compostela
2024
Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol
2013-2023
Cancer Center Amsterdam
2021
Amsterdam UMC Location Vrije Universiteit Amsterdam
2021
University Medical Center
2021
University Hospital and Clinics
2021
Amsterdam University Medical Centers
2021
Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas
2017-2019
Undesired immune responses have drastically hampered outcomes after allogeneic organ transplantation and cell therapy, also lead to inflammatory diseases autoimmunity. Umbilical cord mesenchymal stem cells (UCMSCs) powerful regenerative immunomodulatory potential, their secreted extracellular vesicles (EVs) are envisaged as a promising natural source of nanoparticles increase in control diseases. However, poor EV preparations containing highly-abundant soluble proteins may mask genuine...
Macrophages exert potent effector functions against invading microorganisms but constitute, paradoxically, a preferential niche for many bacterial strains to replicate. Using model of infection by Salmonella Typhimurium, we have identified molecular mechanism regulated the nuclear receptor LXR that limits host macrophages through transcriptional activation multifunctional enzyme CD38. agonists reduced intracellular levels NAD+ in CD38-dependent manner, counteracting pathogen-induced changes...
CD5L (CD5 molecule-like) is a secreted glycoprotein that controls key mechanisms in inflammatory responses, with involvement processes such as infection, atherosclerosis, and cancer. In macrophages, promotes an anti-inflammatory cytokine profile response to TLR activation. the present study, we questioned whether able influence human macrophage plasticity, drive its polarization toward any specific phenotype. We compared CD5L-induced phenotypic functional changes those caused by IFN/LPS,...
CD5L (CD5 molecule-like) is a secreted glycoprotein that participates in host response to bacterial infection. influences the monocyte inflammatory surface molecules lipopolysaccharide (LPS) and lipoteichoic acid (LTA) by inhibiting TNF secretion. Here we studied intracellular events lead macrophage inhibition human CD5L. To accomplish this goal, performed functional analyses with monocytic THP1 macrophages, as well peripheral blood monocytes. Inhibition of phosphatidylinositol 3-kinase...
CD5-like (CD5L) is a soluble scavenger cysteine-rich protein that modulates inflammatory responses. We studied the involvement of CD5L in liver cancer. Immunohistochemistry (IHC) 60 hepatocellular carcinomas and 34 adjacent nontumor livers, showed staining was higher tumor than tissue (Mann-Whitney test; P = 0.0039). High correlated with elevated proliferation (Ki67, linear regression; < 0.0001) lower patient event-free survival (log-rank; 0.0185). Accordingly, expression detected cancer...
ABSTRACT Among the leguminous trees native to Uruguay, Parapiptadenia rigida (Angico), a Mimosoideae legume, is one of most promising species for agroforestry. Like many other legumes, it able establish symbiotic associations with rhizobia and belongs group known as nitrogen-fixing trees, which are major components agroforestry systems. Information about rhizobial symbionts this genus scarce, thus, aim work was identify characterize associated P. . A collection Angico-nodulating isolates...
Reprogramming of immunosuppressive tumor-associated macrophages (TAMs) presents an attractive therapeutic strategy in cancer. The aim this study was to explore the role macrophage CD5L protein TAM activity and assess its potential as a target.Monoclonal antibodies (mAbs) against recombinant were raised by subcutaneous immunization BALB/c mice. Peripheral blood monocytes isolated from healthy donors stimulated with IFN/LPS, IL4, IL10, conditioned medium (CM) different cancer cell lines...
Apoptosis inhibitor of macrophages (AIM), a scavenger protein secreted by tissue macrophages, is transcriptionally regulated the nuclear receptor Liver X Receptor (LXR) and Retinoid (RXR) heterodimer. Given that LXR exerts protective immune response against M. tuberculosis, here we analyzed whether AIM involved in this response. In an experimental murine model serum levels peaked dramatically early after infection with providing vivo biological link to disease. We therefore studied...
Acute‐on‐chronic liver injury is characterized by an important inflammatory response frequently associated with endotoxemia. In this context, acute‐phase proteins such as Pentraxin‐3 (PTX3) are released; however, little known about their role in chronic disease. The aim of study was to elucidate the PTX3 injury. evaluated cultured human cells, tissue slices, and mice acute‐on‐chronic expression assessed serum samples from 54 patients alcoholic hepatitis. up‐regulated animal models strongly...
Chronic hepatic inflammation leads to liver fibrosis, which may progress cirrhosis, a condition with high morbidity. Our aim was assess the as yet unknown role of innate immunity protein CD5L in fibrosis.CD5L measured by ELISA plasma samples from cirrhotic (n = 63) and hepatitis 39) patients, healthy controls 7), immunohistochemistry tissue 12), quantitative RT-PCR mouse cell subsets isolated sorting. Recombinant (rCD5L) administered into murine model CCl4-induced damage, fibrosis immune...
ABSTRACT AIM is expressed by macrophages in response to agonists of the nuclear receptors LXR/RXR. In mice, it acts as an atherogenic factor protecting from apoptotic effects oxidized lipids. humans, detected atherosclerotic lesions, but no role related atherosclerosis has been reported. This study aimed investigate whether hAIM extends beyond inhibiting lipid-induced apoptosis. To accomplish this goal, functional analysis with human monocytic THP1 cells and differentiated peripheral blood...
Scavenger receptors comprise a large family of structurally diverse proteins that are involved in many homeostatic functions. They recognize wide range ligands, from pathogen-associated molecular patterns (PAMPs) to endogenous, as well modified host-derived molecules (DAMPs). The liver deals with blood micro-organisms and DAMPs released injured organs, thus performing vital metabolic clearance functions require the uptake nutrients toxins. Many cell types, including hepatocytes Kupffer...
Abstract The current spotlight of cancer therapeutics is shifting towards personalized medicine with the widespread use monoclonal antibodies (mAbs). Despite their increasing potential, mAbs have an intrinsic limitation related to inability cross cell membranes and reach intracellular targets. Nanotechnology offers promising solutions overcome this limitation, however, formulation challenges remain. These are limited loading capacity (often insufficient achieve clinical dosing), complex...
The Kirsten Rat Sarcoma Virus (KRAS) oncoprotein, one of the most prevalent mutations in cancer, has been deemed undruggable for decades. hypothesis this work was that delivering anti-KRAS monoclonal antibody (mAb) at intracellular level could effectively target KRAS oncoprotein. To reach goal, we designed and developed tLyP1-targeted palmitoyl hyaluronate (HAC16)-based nanoassemblies (HANAs) adapted association bevacizumab as a model mAb. Selected candidates with adequate physicochemical...
Santos-García D, Sanjurjo LF, Macías M, Llaneza Carpintero P, de la Fuente-Fernández R. Long-term exposure to duodenal levodopa/carbidopa infusion therapy improves quality of life in relation especially mobility, activities daily living, and emotional well-being. Acta Neurol Scand: 2012: 125: 187–191. © 2011 John Wiley & Sons A/S. Background – Continuous levodopa (DLI) is an effective that (QoL) advanced Parkinson′s disease (PD). However, which aspects improve the patients their QoL has been...
Atherosclerosis, a process in which macrophages play key role, is accelerated diabetes. Elevated concentrations of serum-oxidized low-density lipoproteins (oxLDL) represent common feature both conditions. The main goal this study was to determine the contribution oxLDL inflammatory response exposed diabetic-mimicking THP1 cells and peripheral blood monocytes purified from non-diabetic healthy donors were cultured under normal (5 mM) or high glucose (HG) (15 with oxLDL. Then, foam cell...
The aim of this study was to determine whether plasma concentrations sCD36 (soluble CD36) are associated with the presence type 1 or 2 diabetes. Plasma levels were analysed in 1023 subjects (225 diabetes (T1D) patients, 276 (T2D) and 522 non-diabetic control subjects) using an enzyme-linked immunosorbent assay (ELISA). Multinomial logistic regression models performed evaluate associations its association types. There no significant differences (p = 0.144) among groups, neither head-to-head...
Abstract Galectins are versatile glycan-binding proteins involved in immunomodulation. Evidence suggests that galectins can control the immunoregulatory function of cytokines and chemokines through direct binding. Here, we report on an inverse mechanism which immunomodulatory functions galectins. We show existence several specific galectin-chemokine binding pairs, including galectin-1/CXCL4. NMR analyses CXCL4 induces changes galectin-1 carbohydrate site. Consequently, alters affinity...
This study aimed to determine the association of fatty acid transporter plasma soluble cluster differentiation 36 (sCD36) with subclinical carotid atherosclerosis (SCA). A cross-sectional was conducted in 1023 subjects, 225 type 1 diabetes (T1D), 276 2 (T2D) and 522 who were nondiabetic. Carotid atherosclerotic plaque (CAP) presence determined using B-mode ultrasound imaging. sCD36 analysed by ELISA, CD36 surface receptor mRNA expression measured flow cytometry real-time PCR. Logistic...
Abstract The stroma of solid tumors is populated by myeloid cells, which mostly represent macrophages. Tumor-associated macrophages (TAMs), strongly influenced cancer cell-derived factors, are key drivers immunosuppression and support tumor growth spread to distant sites. Increasing evidence demonstrates their ability hamper patients' response most treatments currently applied in the clinic, including immunotherapy. Therefore, strategies counteract negative effects TAMs nowadays gaining...
<h3>Background</h3> Toxic epidermal necrolysis (TEN) is a rare but severe cutaneous adverse reaction with high rate of mortality and morbidity. <h3>Purpose</h3> To report case TEN after abiraterone zoledronic acid exposure. <h3>Material methods</h3> Retrospective review the electronic medical record (Ianus) records chemotherapy ordered dispensed (Farmis Silicon). A literature search was conducted keyword terms "abiraterone AND toxic necrolysis" "zoledronic in PubMed EudraVigilance database...
<h3>Background</h3> Desensitisation has been used for some decades now so that patients can be treated with the drug causing hypersensitivity reaction when an alternative similar efficacy/safety is not available. <h3>Purpose</h3> To describe pemetrexed desensitisation protocol and our experience in one patient a previous anaphylactic to pemetrexed. <h3>Material methods</h3> A 43-year-old man stage IV lung adenocarcinoma was cisplatin-pemetrexed, followed by monotherapy 880 mg. During...