A5085665210- Pituitary Gland Disorders and Treatments
- Glioma Diagnosis and Treatment
- Hedgehog Signaling Pathway Studies
- Adrenal and Paraganglionic Tumors
- Cancer, Hypoxia, and Metabolism
- Head and Neck Surgical Oncology
- Epigenetics and DNA Methylation
- Growth Hormone and Insulin-like Growth Factors
- Immune cells in cancer
- Thyroid Cancer Diagnosis and Treatment
- Peroxisome Proliferator-Activated Receptors
- Fibroblast Growth Factor Research
- Neuroblastoma Research and Treatments
- Chromatin Remodeling and Cancer
- Neurofibromatosis and Schwannoma Cases
- Genetic Syndromes and Imprinting
- Histone Deacetylase Inhibitors Research
- NF-κB Signaling Pathways
- Intracerebral and Subarachnoid Hemorrhage Research
- Wnt/β-catenin signaling in development and cancer
- Brain Metastases and Treatment
- Genetic and Kidney Cyst Diseases
- TGF-β signaling in diseases
- Connective tissue disorders research
- Protein Degradation and Inhibitors
Friedrich-Alexander-Universität Erlangen-Nürnberg
2007-2024
Universitätsklinikum Erlangen
2010-2024
Comprehensive Cancer Center Erlangen
2024
Klinikum Coburg
2020
Institute of Neuroimmunology of the Slovak Academy of Sciences
2015
Universität Hamburg
2013
University Medical Center Hamburg-Eppendorf
2013
International Neuroscience Institute
2011-2013
Neurology, Inc
2007
Craniopharyngiomas (CP) are rare epithelial tumors of the sellar region. Two subtypes, adamantinomatous (adaCP) and papillary CP (papCP), were previously identified based on histomorphological epidemiological aspects. Recent data indicates that both variants defined by specific genetic alterations, influenced distinct molecular pathways particular origins. The fact is an uncommon tumor entity renders studies large cohorts difficult exceptional. In order to achieve further insights...
Adamantinomatous craniopharyngiomas (ACPs) are clinically challenging tumours, the majority of which have activating mutations in CTNNB1. They histologically complex, showing cystic and solid components, latter comprised different morphological cell types (e.g. β-catenin-accumulating cluster cells palisading epithelium), surrounded by a florid glial reaction with immune cells. Here, we carried out RNA sequencing on 18 ACP samples integrated these data an existing transcriptomic dataset. No...
The term atypical pituitary adenoma (APA) was revised in the 2004 World Health Organization (WHO) classification of tumors. However, two four parameters required for diagnosis APAs were formulated rather vaguely (i.e., “extensive” nuclear staining p53; “elevated” mitotic index). Based on a case-control study using representative cohort typical adenomas and selected from German Pituitary Tumor Registry, we aimed to obtain reliable cut-off values both p53 index. In addition, analyzed impact...
Constitutive Wnt signaling caused by mutations in the β-catenin gene is a molecular hallmark of adamantinomatous craniopharyngiomas (adaCP) and promotes infiltration into adjacent brain tissue. Herein, we studied pathogenic role epidermal growth factor receptor (EGFR) activation adaCP whether tumor cell migration can be inhibited tyrosine kinase inhibitor gefitinib.EGFR expression [phosphorylated EGFR (EGFR-P)] was examined cohort 25 surgical samples PCR Western blotting. Regional cellular...
Activating beta-catenin (CTNNB1) mutations can be identified in the majority of adamantinomatous craniopharyngiomas (adaCP), suggesting an aberrant Wnt signaling pathway this histopathologically peculiar tumor entity. However, there is no proven evidence that nuclear translocation associated with CTNNB1 and target gene activation. We performed a laser-microdissection-based study comparing accumulating vs. non-accumulating cells. Mutational analysis expression profiling using real-time...
Aims The differential diagnosis of cystic epithelial masses the sellar region, especially histopathological differentiation craniopharyngiomas and R athke's cleft cysts, poses a challenge even to experienced diagnosticians. Recently, BRAF V 600 E mutations have been described as genetic hallmark papillary craniopharyngiomas. We investigated series 33 cysts determine frequency its suitability an additional diagnostic marker for lesions region. Methods Thirty‐three 18 were analysed mutational...
Adamantinomatous craniopharyngiomas (adaCP) cause hypothalamic pituitary dysfunction. Elucidation of pathomechanisms underlying tumor progression is essential for the development targeted chemotherapeutic treatment options. In order to study mechanisms outgrowth, we implanted human primary adaCP tissue from three different surgical specimens stereotactically into brain immunodeficient mice (n = 20). Three months after inoculation, magnetic resonance imaging and histology confirmed...
Claudins are tight junction proteins expressed in epithelial tissues that play important roles cell polarity and adhesion. Altered distribution of claudin-1(CLDN1) affects mobility tumor invasiveness. Craniopharyngiomas (CPs) represent tumors the sellar region, consisting adamantinomatous (adaCP) papillary (papCP) variants. Their tendency to infiltrate surrounding brain structures complicates successful surgery. Reliable markers required predict behavior establish individualized treatment...
Abstract Hedgehog (HH) signaling is known to be essential during the embryonal development of pituitary gland but knowledge about its role in adult and associated tumors sparse. In this report we investigated effect excess Hh activation murine explants analyzed HH status human adenopituitary lobes a large cohort adenomas. Our data show that led increased proliferation Sox2 + Sox9 stem cells elevated expression levels adrenocorticotropic hormone (Acth), growth (Gh) prolactin (Prl) gland....
Abstract Gliomas are the most common primary tumors of central nervous system, with glioblastomas as malignant entity. Rapid proliferation and diffuse brain invasion these likely to determine unfavorable prognosis. Considering its promigratory properties, transforming growth factor-β (TGF-β) signaling pathway has become a major therapeutic target. Analyses resected glioma tissues revealed an intriguing correlation between tumor grade expression TGF-β1-3 well their receptors I II. Here, we...
A 6-year old patient presented with an intra and suprasellar cystic lesion accompanied impairment of the hypothalamic-pituitary axis partial hypopituitarism. The most likely cause sellar lesions in this age group are adamantinomatous craniopharyngioma (adaCP) or Rathke´s cleft cysts (RCCs). AdaCP characterized by
To assess the clinical relevance of transgenic and patient-derived xenograft models adamantinomatous craniopharyngioma (ACP) using serial magnetic resonance imaging (MRI) high resolution post-mortem microcomputed tomography (μ-CT), with correlation histology human ACP imaging. The growth patterns radiological features tumors arising in Hesx1
Abstract Enhancer of zeste homolog 2 (EZH2) is a core epigenetic regulator, playing crucial role in cell cycle regulation. The protein known to be associated with proliferation and worse outcome several tumor entities. In this study, we immunohistochemically investigated the expression pattern EZH2 large cohort pituitary tumors. These results were correlated clinical features double immunofluorescence stainings (DIS) conducted evaluate co-expression marker Ki-67. Furthermore, analyzed effect...
OBJECTIVE In this study, the authors investigated underlying mechanisms responsible for high tumor recurrence rates of adamantinomatous craniopharyngioma (ACP) after radiotherapy and developed new targeted treatment protocols to minimize recurrence. ACPs are characterized by activation receptor tyrosine kinase epidermal growth factor (EGFR), known mediate radioresistance in various entities. The impact inhibitors (TKIs) gefitinib or CUDC-101 on radiation-induced cell death associated...
Abstract The epithelial cell adhesion molecule (EpCAM) is a type I glycoprotein located on the surface of cells. It strongly expressed in many neoplasms and already used diagnosis distinction various tumour subtypes. Comparative studies about EpCAM expression cystic sellar lesions are lacking. Therefore, we analysed its distribution pattern adamantinomatous (aCP) papillary (pCP) craniopharyngiomas (CP) Rathke’s Cleft Cysts (RCC) using immunohistochemistry gene profiling. Whereas protein was...