A5011601884- Extracellular vesicles in disease
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Cancer Immunotherapy and Biomarkers
- Cytokine Signaling Pathways and Interactions
- Cancer, Hypoxia, and Metabolism
- Nanoplatforms for cancer theranostics
- Glutathione Transferases and Polymorphisms
- Astronomical and nuclear sciences
- Gold and Silver Nanoparticles Synthesis and Applications
- Cancer, Lipids, and Metabolism
- Chemical Synthesis and Characterization
- Maternal and fetal healthcare
- Pregnancy and preeclampsia studies
- Gestational Trophoblastic Disease Studies
- Click Chemistry and Applications
- Metal-Organic Frameworks: Synthesis and Applications
- Colorectal Cancer Screening and Detection
- T-cell and B-cell Immunology
- Ferroptosis and cancer prognosis
- Peroxisome Proliferator-Activated Receptors
- Microtubule and mitosis dynamics
- Genomics, phytochemicals, and oxidative stress
Southern Medical University
2021-2024
Nanfang Hospital
2021-2024
Key Laboratory of Guangdong Province
2023-2024
California NanoSystems Institute
2021-2023
University of California, Los Angeles
2021-2023
APLA Health
2021
Hunan Institute of Science and Technology
2021
Northeast Normal University
2013
Peking University
2006
The sensitivity of current surveillance methods for detecting early-stage hepatocellular carcinoma (HCC) is suboptimal. Extracellular vesicles (EVs) are promising circulating biomarkers early cancer detection. In this study, we aim to develop an HCC EV-based surface protein assay detection HCC.Tissue microarray was used evaluate four potential HCC-associated markers. An EV assay, composed covalent chemistry-mediated purification and real-time immuno-polymerase chain reaction readouts,...
Well-preserved molecular cargo in circulating extracellular vesicles (EVs) offers an ideal material for detecting oncogenic gene alterations cancer patients, providing a noninvasive diagnostic solution detection of disease status and monitoring treatment response. Therefore, technologies that conveniently isolate EVs with sufficient efficiency are desperately needed. Here, lipid labeling click chemistry-based EV capture platform ("Click Beads"), which is message ribonucleic acid (mRNA)...
Abstract Placenta accreta spectrum (PAS) is a high-risk obstetrical condition associated with significant morbidity and mortality. Current clinical screening modalities for PAS are not always conclusive. Here, we report nanostructure-embedded microchip that efficiently enriches both single clustered circulating trophoblasts (cTBs) from maternal blood detecting PAS. We discover uniquely high prevalence of cTB-clusters in subsequently optimize the device to preserve intactness these clusters....
Optimizing outcomes in prostate cancer (PCa) requires precision characterization of disease status. This effort was directed at developing a PCa extracellular vesicle (EV) Digital Scoring Assay (DSA) for detecting metastasis and monitoring progression PCa. EV DSA is comprised an purification device (i.e., Click Chip) reverse-transcription droplet digital PCR that quantifies 11 PCa-relevant mRNA purified PCa-derived EVs. A Met score computed each plasma sample based on the expression 11-gene...
The abnormal expression of glutathione S-transferase P1 (GSTP1) is associated with the progression several tumor types. However, its role and molecular mechanism in colorectal cancer (CRC) are largely unknown.To examine effect GSTP1 CRC determine possible mechanisms.In present study, immunohistochemistry (IHC) quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis were used to detect signal transducer activator 3 (STAT3) tissues. Western blotting was applied proteins...
It is well-established that the combined use of nanostructured substrates and immunoaffinity agents can enhance cell-capture performance substrates, thus offering a practical solution to effectively capture circulating tumor cells (CTCs) in peripheral blood. Developing along this strategy, study first demonstrated top-down approach for fabrication tetrahedral DNA nanostructure (TDN)-NanoGold through hierarchical integration three functional constituents at various length-scales: macroscale...
Waveband-dependent photochemical reduction of graphene oxide (GO) and Ag + in water has been studied. The microstructure GO is suggested to be responsible for the waveband-dependent photoreduction.
Abstract Background Dysfunction of CD8 + T cells in the tumor microenvironment (TME) contributes to immune escape and immunotherapy tolerance. The effects hormones such as leptin, steroid hormones, glucocorticoids on cell function have been reported previously. However, mechanism underlying thyroid‐stimulating hormone (TSH)/thyroid‐stimulating receptor (TSHR) signaling exhaustion evasion remain poorly understood. This study was aimed at investigating TSH/TSHR colorectal cancer (CRC). Methods...
Neoadjuvant chemoradiotherapy (nCRT) has become the standard treatment for patients with locally advanced rectal cancer (LARC). However, 20-40% of LARC show little to no response nCRT. Thus, comprehensively understanding tumor microenvironment (TME), which might influence therapeutic efficacy, and identifying robust predictive biomarkers is urgently needed. Pre-treatment biopsy specimens from were evaluated in detail through digital spatial profiling (DSP), public RNA sequencing datasets,...
Numerous studies in hepatocellular carcinoma (HCC) have proposed tissue‐based gene signatures for individualized prognostic assessments. Here, we develop a novel circulating tumor cell (CTC)–based transcriptomic profiling assay to translate messenger RNA (mRNA) into liquid biopsy setting noninvasive HCC prognostication. The HCC‐CTC mRNA scoring system combines the NanoVelcro CTC Assay enriching CTCs and NanoString nCounter platform quantifying Risk Score (RS) panel enriched CTCs. role of RS...
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR/Cas9) is an efficient and precise gene-editing technology that offers a versatile solution for establishing treatments directed at genetic diseases. Currently, CRISPR/Cas9 delivery into cells relies primarily on viral vectors, which suffer from limitations in packaging capacity safety concerns. These issues with nonviral strategy are addressed, where Cas9•sgRNA ribonucleoprotein (RNP)...
Transcriptomic profiling of tumor tissues introduces a large database, which has led to improvements in the ability cancer diagnosis, treatment, and prevention. However, performing transcriptomic clinical setting is very challenging since procurement inherently limited by invasive sampling procedures. Here, we demonstrated feasibility purifying hepatocellular carcinoma (HCC) circulating cells (CTCs) from patient samples with improved molecular integrity using Click Chips conjunction...
Abstract Abnormal expression of glutathione S-transferase Pi 1 (GSTP1) is associated with the progression several tumor types. However, its role and molecular mechanism in colorectal cancer (CRC) largely unknown. In present study, immunohistochemistry (IHC) quantitative-reverse transcription PCR (qRT-PCR) were used to detect GSTP1 signal transducer activator 3 (STAT3) CRC tissues. Western blotting was applied proteins Janus kinase (JAK)-STAT3 pathway. The interaction co-localization STAT3...
Extracellular Vesicle In article number 2105853 by Renjun Pei, Shaohua Lu, Hsian-Rong Tseng, Yazhen Zhu, and co-workers, an efficient, convenient, rapid extracellular vesicle (EV) enrichment platform called EV Click Beads is developed combining lipid labelling click chemistry-based capturing. provides a non-invasive diagnostic solution for detection of disease status monitoring treatment response cancer patients, including Ewing sarcoma, the second most common type bone in children.
Abstract Background Many solid tumors rely heavily on the regulation and organization of leader follower cells during collective invasion. However, cells’ specific biomarkers mechanisms in colorectal cancer (CRC) invasion are unclear. This study aimed to identify reveals their molecular CRC metastasis. Methods The 3D photoconvertible spheroid model vitro was constructed isolate cells. RNA-Seq, functional, animal experiments revealed that GLUT1, PLOD2, CCL5 were required for Results...
Abstract Abnormal metabolic process is involved in sequential steps of the pre-metastatic cascade and overt metastasis at distant organ. However, mechanisms underlying lipid reprogramming macrophages regulates formation pre-metastic niche (PMN) during liver remain unknown. Here, we found that fatty acid binding protein 7 (FABP7) was up-regulated leading to droplet accumulation PMN colorectal cancer (CRC) pancreatic ductal adenocarcinoma (PDAC). This partially mediated by hypoxia inducible...
Sensitivity of the direct nuclear reaction to exotic structure unstable nuclei is outlined. The transfer induced by 6 He on a 9 Be target at 25 MeV/u described. theoretical analysis using CRC model supports di-neutron configuration two valence neutrons in . Preliminary calculation for 17 Ne were introduced which indicates advantage clarify s-wave or d-wave protons.
Abstract NaY zeolite was synthesized from kaolin/dimethyl sulfoxide (DMSO) intercalation composites using an in situ crystallization technique. The effects of the ratios and amounts kaolin/DMSO composite on synthesis molecular sieve were studied. samples characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, differential thermal analysis, N 2 adsorption–desorption scanning electron microscopy. In system, when added, pure formed. By increasing amount crystallinity...
Abstract Background and Aims Numerous studies in hepatocellular carcinoma (HCC) have proposed tissue-based gene signatures for individualized prognostic assessments. In this study, we aim to develop the HCC circulating tumor cell (CTC) mRNA Scoring System HCC-CTC Risk Score (RS) panel translate into a liquid biopsy setting non-invasive prognostication. Methods We developed CTC by combining NanoVelcro Assay enriching CTCs NanoString nCounter platform quantifying RS enriched CTCs. The role of...